FastQCFastQC Report
Thu 26 May 2016
SRR1926837_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926837_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503245
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT15060.2992578167691681No Hit
GGTATCAACGCAGAGTACTTTTTTT10660.21182525410088524No Hit
CTGTAGGACGTGGAATATGGCAAGA8360.1661218690697374No Hit
TATCAACGCAGAGTACTTTTTTTTT7720.15340440540889627No Hit
GTCCTACAGTGGACATTTCTAAATT7650.15201343282099175No Hit
CTTTAGGACGTGAAATATGGCGAGG6620.13154626474182557No Hit
GTCCTAAAGTGTGTATTTCTCATTT6480.12876431956601656No Hit
GTCCTACAGTGTGCATTTCTCATTT5410.10750231000804777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG607.724884E-817.40272918
CCAACGA555.9396734E-717.25890419
ACCGTCT400.00530761314.2357668
CTTGGAC551.9282008E-413.8415143
AATGCCG551.9693367E-413.80712218
TAGGACT707.121409E-613.5929894
GTATAAA1009.626092E-913.3370741
TAGAACT655.357757E-513.1747454
GGCGAGG1600.013.05204619
TAATACT905.2779797E-712.68678954
GTATAAT1001.3837598E-712.3844261
GTGTAAC550.003000254312.1246131
TAATAGT550.003027265212.1101184
CTAATAC802.808402E-511.8950513
TTGGACA802.8112681E-511.8938664
TATAGTG802.8112681E-511.8938665
TCAAGGC657.8942976E-411.7120513
AACTATC658.060837E-411.6829517
ATGCCGG658.060837E-411.68295119
AGGACCT3600.011.6295585
Produced by FastQC (version 0.11.5)