FastQCFastQC Report
Thu 26 May 2016
SRR1926836_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926836_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412551
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44350.10050875332659046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18350.011.0200081
TACGACT1002.3434173E-510.4730574
GTCCTAG5700.010.2079161
GTATCAA38850.010.0664181
GTATAAT5300.09.5385461
CAATACG1201.6582575E-59.5209624
CTTATAC4050.09.1786743
TATTCCG1054.4126823E-49.06448655
TATACCG1054.4126823E-49.06448655
TTATACG950.00178860729.0198574
ACCGTGC2850.09.0088888
GTTCGTA850.00719621648.9774541
TCTAACG850.00723670478.9713172
TAGACAG6850.08.8924615
GTATAAA6550.08.88322451
GTCTAGG4200.08.8572211
GACCGTG3500.08.701717
GTCCTAA3850.08.6714051
TGGACCG3300.08.6524665
TCAATAC4200.08.6239183
Produced by FastQC (version 0.11.5)