##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926836_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4412551 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.880877977387684 31.0 28.0 31.0 16.0 34.0 2 27.99456346226933 31.0 28.0 31.0 16.0 34.0 3 27.473277249373435 31.0 27.0 31.0 12.0 34.0 4 31.166230373314665 35.0 32.0 37.0 17.0 37.0 5 31.491662079373132 35.0 32.0 37.0 17.0 37.0 6 30.644671529008956 35.0 30.0 37.0 11.0 37.0 7 30.7646780739758 35.0 32.0 37.0 11.0 37.0 8 30.591008013278486 35.0 31.0 37.0 10.0 37.0 9 32.69238701150423 37.0 32.0 39.0 17.0 39.0 10 32.88496518227211 37.0 33.0 39.0 17.0 39.0 11 33.05164926139097 37.0 33.0 39.0 17.0 39.0 12 32.91360439800016 37.0 33.0 39.0 15.0 39.0 13 33.00590814701065 38.0 33.0 39.0 16.0 39.0 14 34.009637735631834 39.0 33.0 40.0 16.0 41.0 15 33.54783072195653 39.0 33.0 40.0 10.0 41.0 16 33.62833222777482 39.0 33.0 40.0 10.0 41.0 17 33.852504820907455 39.0 33.0 40.0 10.0 41.0 18 33.89544778066021 39.0 33.0 41.0 10.0 41.0 19 33.23494708616399 39.0 32.0 40.0 8.0 41.0 20 33.510829903155795 39.0 33.0 40.0 8.0 41.0 21 33.50348290591995 39.0 33.0 40.0 2.0 41.0 22 33.51185742669037 39.0 33.0 40.0 2.0 41.0 23 33.485434389313575 39.0 33.0 40.0 2.0 41.0 24 32.13733461664239 38.0 31.0 40.0 2.0 41.0 25 32.70519570198736 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.445743263861214 1101 2 -0.5762635794842055 1101 3 -1.4250476311381242 1101 4 -0.9259725142821473 1101 5 -0.5979394562659515 1101 6 -2.6651344666245222 1101 7 -1.0200894922490527 1101 8 -0.5617979131838382 1101 9 -0.6222976332142025 1101 10 -9.144396748765004 1101 11 -4.20146960217264 1101 12 -1.8272178633202145 1101 13 -1.165669116694616 1101 14 -0.9192143120879166 1101 15 -2.2889881404526307 1101 16 -0.7886547260628021 1101 17 -0.6743313286691475 1101 18 -0.5671606226552228 1101 19 -1.1182876664132166 1101 20 -0.45558095224963324 1101 21 -0.5628609913757856 1101 22 -0.3218181344861435 1101 23 -0.46990959037775326 1101 24 -0.6565553632554604 1101 25 -0.49760572341639175 1102 1 -0.12869090707820874 1102 2 -0.19540965939920696 1102 3 -0.5174186450548639 1102 4 -0.5342272355297268 1102 5 -0.043952840710311136 1102 6 -1.0445408494620523 1102 7 -0.1288100659914022 1102 8 0.47644593725616247 1102 9 0.32267126618218356 1102 10 0.26549705063063556 1102 11 0.09249251027539884 1102 12 0.1662795819049876 1102 13 0.04451092587337513 1102 14 -0.20122149937619582 1102 15 0.322126622857283 1102 16 0.23228838382359385 1102 17 0.08414643040793379 1102 18 -0.14336857785156099 1102 19 0.321693233503062 1102 20 0.19158388086587053 1102 21 -0.01302894512589603 1102 22 0.06144026265771885 1102 23 -0.3215035553076149 1102 24 -3.6266271513053248 1102 25 -1.9459487168354777 1103 1 -0.17362125125461603 1103 2 -0.3244742759844499 1103 3 -0.08502323344633567 1103 4 -0.34806898170781153 1103 5 -0.41871707648183687 1103 6 0.1702382743572315 1103 7 0.08951680298859088 1103 8 0.22868197685495062 1103 9 0.04985302383979473 1103 10 0.053697216620939514 1103 11 -0.09739438050378624 1103 12 -0.27039098264064165 1103 13 -0.16328398872438044 1103 14 -0.054629677490481754 1103 15 0.15223372443494299 1103 16 0.14712756967224294 1103 17 -0.11358813027559478 1103 18 -0.21742442156872954 1103 19 -1.910431819761122 1103 20 -0.6566437011773019 1103 21 -0.44584780580330374 1103 22 -0.29556563629674315 1103 23 -0.31020866768999866 1103 24 1.2698941237398813 1103 25 0.5730048596469004 1104 1 -0.4998018060877243 1104 2 -0.329680104068089 1104 3 -1.5957818139905768 1104 4 -0.8364941961327546 1104 5 -0.5084559126242034 1104 6 -1.8849170743735257 1104 7 -0.4502435489435257 1104 8 0.11713769168514432 1104 9 0.036381585932474536 1104 10 0.155879319645166 1104 11 -0.011999345875189249 1104 12 0.020178693998317954 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0.41720555449142793 2215 4 0.7260947740813677 2215 5 0.6101109550148465 2215 6 1.3322844579539819 2215 7 0.8022219041412448 2215 8 -0.40288450645287455 2215 9 0.04921682306726183 2215 10 -0.21673754401546574 2215 11 -0.18052627751711015 2215 12 -0.469379333461049 2215 13 -0.16774242322563282 2215 14 -0.044573534816649385 2215 15 0.0160441140351395 2215 16 0.13940574412602302 2215 17 -0.35069795030437234 2215 18 -1.0547224523556125 2215 19 0.1763401187802458 2215 20 -3.8145065784860606 2215 21 -2.12853686455653 2215 22 -1.135031901464565 2215 23 -1.0309441681183316 2215 24 -1.0401407004838923 2215 25 -0.8228159145816392 2216 1 -0.3229222023097016 2216 2 -0.8530719141609531 2216 3 0.09981864993571676 2216 4 -0.08770947401342255 2216 5 -0.3884316179318361 2216 6 -1.4403724655214312 2216 7 -9.47884492890892 2216 8 -10.761426558767887 2216 9 -5.747354482598393 2216 10 -3.3968245168340516 2216 11 -2.3319915584563127 2216 12 -1.9093895151580824 2216 13 -1.6141698001978746 2216 14 -1.7085593583305752 2216 15 -2.774110372139326 2216 16 -1.787207293379204 2216 17 -2.0417771521145305 2216 18 -1.7505496168527444 2216 19 -1.152140886396893 2216 20 -2.1361802904197376 2216 21 -4.5937696009193765 2216 22 -3.301363437115505 2216 23 -3.1565979041937418 2216 24 -3.201519368673921 2216 25 -2.7278275888244536 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 287604.0 3 19.0 4 1965.0 5 12202.0 6 15680.0 7 12506.0 8 10299.0 9 9382.0 10 8967.0 11 8707.0 12 8596.0 13 8576.0 14 8889.0 15 9232.0 16 9794.0 17 10598.0 18 12095.0 19 15059.0 20 19460.0 21 25336.0 22 31139.0 23 37644.0 24 43124.0 25 49791.0 26 56569.0 27 62214.0 28 68981.0 29 81004.0 30 96582.0 31 117426.0 32 149882.0 33 194996.0 34 259983.0 35 356608.0 36 507361.0 37 783372.0 38 1020909.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.75093829359789 17.203782880019695 12.02987096220345 26.015407864178965 2 16.02846673408804 19.231450821842618 38.649758832831225 26.090323611238116 3 19.016796751882183 23.700318335695872 28.54297373055444 28.739911181867505 4 13.280425776264263 15.879751043893888 35.92101694269492 34.918806237146924 5 14.306173696968983 36.50318416183803 34.91779027736913 14.272851863823869 6 33.464382553709854 34.85588137023203 18.22893597613913 13.450800099918986 7 30.104245238195915 30.47975179012371 21.118799470226136 18.29720350145424 8 26.910519669897155 34.65586630402786 19.796350956473113 18.63726306960187 9 26.86208860740844 15.225331064545092 18.784596508462737 39.12798381958373 10 15.462955531947598 27.984974638070508 33.224853103940156 23.327216726041737 11 35.631717819236165 22.11127397992732 22.759774943351648 19.49723325748487 12 23.83067161158038 24.009396605381106 29.895829508369136 22.264102274669376 13 28.98067841442642 20.995012358606996 24.92231813694991 25.101991090016675 14 22.54596474685531 20.147785316894108 26.3061717369648 31.00007819928578 15 24.60352196747485 27.951777790521064 22.70787996760586 24.736820274398223 16 24.35125451440905 26.943312418526432 24.818420624941776 23.887012442122742 17 23.528710427763002 26.935301293683995 25.799332780566044 23.736655497986963 18 23.509175143701608 26.8690275435638 26.20446426952438 23.417333043210213 19 24.87586435921528 26.196094076737985 25.93389330851943 22.994148255527307 20 24.72590128842431 25.972370151749065 25.97397946381802 23.327749096008613 21 24.736173458343863 25.64489862066074 25.8276545566021 23.791273364393305 22 24.658508280029558 25.59753117760924 25.747922626036416 23.996037916324784 23 24.366281432629076 25.684933757984595 25.641753878312525 24.307030931073808 24 24.54977182031944 25.928732325537858 25.539079311875177 23.982416542267526 25 24.359316383526423 25.624488739403915 25.795594585448544 24.220600291621118 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 866.0 1 866.0 2 1121.0 3 1376.0 4 1376.0 5 1376.0 6 2080.5 7 2785.0 8 2785.0 9 2785.0 10 3996.5 11 5208.0 12 5208.0 13 5208.0 14 8826.0 15 12444.0 16 12444.0 17 12444.0 18 22785.5 19 33127.0 20 33127.0 21 33127.0 22 51877.0 23 70627.0 24 70627.0 25 70627.0 26 101485.0 27 132343.0 28 132343.0 29 132343.0 30 172401.0 31 212459.0 32 212459.0 33 212459.0 34 260668.5 35 308878.0 36 308878.0 37 308878.0 38 362691.0 39 416504.0 40 416504.0 41 416504.0 42 464175.5 43 511847.0 44 511847.0 45 511847.0 46 550298.5 47 588750.0 48 588750.0 49 588750.0 50 598659.0 51 608568.0 52 608568.0 53 608568.0 54 573977.5 55 539387.0 56 539387.0 57 539387.0 58 476989.5 59 414592.0 60 414592.0 61 414592.0 62 345763.5 63 276935.0 64 276935.0 65 276935.0 66 217106.0 67 157277.0 68 157277.0 69 157277.0 70 115646.5 71 74016.0 72 74016.0 73 74016.0 74 51940.5 75 29865.0 76 29865.0 77 29865.0 78 20150.5 79 10436.0 80 10436.0 81 10436.0 82 6787.0 83 3138.0 84 3138.0 85 3138.0 86 1999.5 87 861.0 88 861.0 89 861.0 90 527.0 91 193.0 92 193.0 93 193.0 94 116.0 95 39.0 96 39.0 97 39.0 98 34.5 99 30.0 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21178225475467594 2 0.12333001930175992 3 0.12618551037710385 4 0.07023148287691179 5 0.023319843781975554 6 0.02214138714770662 7 0.3103193594816241 8 0.30177554888317437 9 0.01808477681051165 10 0.03646416777959054 11 0.015501237266152846 12 0.016883657548660627 13 0.014640057418033241 14 0.01690632017624272 15 0.014549406907704864 16 0.019127257679288013 17 0.018334065713914695 18 0.01430011800430182 19 0.023705108450871162 20 0.016498392879765015 21 0.01486668369385419 22 0.0165663807625113 23 0.017540873748541377 24 0.015342598873078182 25 0.015002659459346758 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4412551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.3513569075429 #Duplication Level Percentage of deduplicated Percentage of total 1 88.90450683830632 70.54693252815531 2 7.239040790033139 11.488554187963617 3 1.8436569037376271 4.388900309506197 4 0.7565163381657939 2.401223918247253 5 0.3625457991748008 1.438425055282499 6 0.22452765919473888 1.0689944652226127 7 0.1432479298875163 0.795684232753969 8 0.10228767166098518 0.6493332432969916 9 0.07366049063454616 0.5260553894093933 >10 0.32931148558447093 4.465717151465891 >50 0.01450783919298099 0.7691649013107694 >100 0.005450755056307658 0.730941827091922 >500 3.4130755148034414E-4 0.1826988440284762 >1k 3.9819181940064136E-4 0.5473739462650541 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4435 0.10050875332659046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.2662627582094803E-5 0.0 5 0.0 4.5325255164189607E-5 0.0 2.2662627582094803E-5 0.0 6 0.0 4.5325255164189607E-5 0.0 4.5325255164189607E-5 0.0 7 0.0 4.5325255164189607E-5 0.0 4.5325255164189607E-5 0.0 8 0.0 4.5325255164189607E-5 0.0 4.5325255164189607E-5 0.0 9 0.0 4.5325255164189607E-5 0.0 6.79878827462844E-5 0.0 10 0.0 4.5325255164189607E-5 0.0 6.79878827462844E-5 0.0 11 0.0 4.5325255164189607E-5 0.0 1.13313137910474E-4 0.0 12 0.0 4.5325255164189607E-5 0.0 1.13313137910474E-4 4.5325255164189607E-5 13 0.0 4.5325255164189607E-5 0.0 1.13313137910474E-4 9.065051032837921E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1835 0.0 11.020008 1 TACGACT 100 2.3434173E-5 10.473057 4 GTCCTAG 570 0.0 10.207916 1 GTATCAA 3885 0.0 10.066418 1 GTATAAT 530 0.0 9.538546 1 CAATACG 120 1.6582575E-5 9.520962 4 CTTATAC 405 0.0 9.178674 3 TATTCCG 105 4.4126823E-4 9.0644865 5 TATACCG 105 4.4126823E-4 9.0644865 5 TTATACG 95 0.0017886072 9.019857 4 ACCGTGC 285 0.0 9.008888 8 GTTCGTA 85 0.0071962164 8.977454 1 TCTAACG 85 0.0072367047 8.971317 2 TAGACAG 685 0.0 8.892461 5 GTATAAA 655 0.0 8.8832245 1 GTCTAGG 420 0.0 8.857221 1 GACCGTG 350 0.0 8.70171 7 GTCCTAA 385 0.0 8.671405 1 TGGACCG 330 0.0 8.652466 5 TCAATAC 420 0.0 8.623918 3 >>END_MODULE