FastQCFastQC Report
Thu 26 May 2016
SRR1926836_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926836_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4412551
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75210.170445622044935No Hit
GGTATCAACGCAGAGTACTTTTTTT46160.10461068891894962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.011.0362261
GTATCAA42600.010.4239391
TACGGGT750.002603469210.1546344
GCGCTTA856.6624105E-410.0482099
TAACGCA951.6108788E-410.0210214
ATACCGT1356.2696563E-79.8724496
TATACCG1201.6603024E-59.519975
ATACCGC800.0044410469.5198626
GTCTAAT2950.09.3676671
GTATTAA3800.09.2784111
ATCGCTC1252.7926086E-59.1099638
TCTATAC4100.08.8240893
TAATCCC5150.08.6881285
GTCCTAA4000.08.57626151
TAGACTG5000.08.5679735
ACAACGC1451.7143175E-58.5078698
CTAGGAC5400.08.4628693
GCACCGT3150.08.46216
GTCTAAA4000.08.3380321
AGCACCG3550.08.3132135
Produced by FastQC (version 0.11.5)