FastQCFastQC Report
Thu 26 May 2016
SRR1926835_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926835_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423882
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29280.20563501750847335No Hit
GGTATCAACGCAGAGTACTTTTTTT18570.1304181104894928No Hit
TATCAACGCAGAGTACTTTTTTTTT17370.1219904458375062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGC400.005343677514.2237919
GTATCAA13450.010.9880231
TAGGACT1158.5423744E-710.7651274
GTTCTAG1605.0931703E-1010.7266141
GGTATCA6550.010.6265351
TATGTAC1553.4779077E-910.4515833
CCTAGAC1201.463708E-610.3234993
GTGTAAG2600.010.2682121
CTAGGAC1605.860784E-910.124973
GTCCTAA1701.4624675E-910.0956371
GTCCTAG2002.3646862E-1110.0115071
ATTAGAC1252.4689944E-69.910563
CAATACT1252.4902474E-69.9039174
GTATTAG1401.0063486E-69.5347681
GTCCTAC2002.4738256E-109.5347681
GTCTAGG1502.5090048E-79.5347681
GTGTACG800.0043809889.5347681
CCAATAC1304.072448E-69.5293843
TAGAACC1002.6883697E-49.5229984
TTAAACC1502.5544432E-79.5229984
Produced by FastQC (version 0.11.5)