##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926835_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1423882 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.564850879497037 31.0 28.0 31.0 16.0 34.0 2 27.50986106994821 31.0 28.0 31.0 16.0 34.0 3 26.94870853062262 31.0 27.0 31.0 10.0 34.0 4 31.134506932456482 35.0 32.0 37.0 17.0 37.0 5 30.62319279266119 35.0 31.0 37.0 10.0 37.0 6 30.128278185973276 35.0 30.0 37.0 10.0 37.0 7 30.198956093271775 35.0 30.0 37.0 10.0 37.0 8 30.19967314707258 35.0 30.0 37.0 10.0 37.0 9 32.30588349315463 37.0 32.0 39.0 11.0 39.0 10 32.54492928487051 37.0 32.0 39.0 10.0 39.0 11 32.719831418614746 37.0 33.0 39.0 10.0 39.0 12 32.61899441105373 37.0 33.0 39.0 10.0 39.0 13 32.68721003566307 37.0 33.0 39.0 10.0 39.0 14 33.64460959545805 39.0 33.0 40.0 10.0 41.0 15 33.20803479501813 38.0 32.0 40.0 8.0 41.0 16 33.268544022608616 38.0 33.0 40.0 8.0 41.0 17 33.471743445032665 38.0 33.0 40.0 2.0 41.0 18 33.50862009632821 39.0 33.0 40.0 2.0 41.0 19 32.87024978193418 39.0 32.0 40.0 2.0 41.0 20 33.17522659883333 39.0 33.0 40.0 2.0 41.0 21 33.16268693613656 39.0 33.0 40.0 2.0 41.0 22 33.178754981100965 39.0 33.0 40.0 2.0 41.0 23 33.15235672618939 39.0 33.0 40.0 2.0 41.0 24 31.83749004482113 38.0 31.0 40.0 2.0 41.0 25 32.393482044158155 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.688171161362721 1101 2 -0.7593124922259662 1101 3 -1.7221467992724833 1101 4 -0.9314277409097791 1101 5 -1.113125057513784 1101 6 -2.985039470042949 1101 7 -1.481244951198505 1101 8 -0.6253616675695213 1101 9 -0.7773154369440682 1101 10 -9.45330940715315 1101 11 -4.34892858142366 1101 12 -1.8834486613056036 1101 13 -1.220440191173573 1101 14 -1.0894907417751014 1101 15 -2.5473430253824496 1101 16 -0.8109730066429819 1101 17 -0.7244749143562004 1101 18 -0.7190455885455975 1101 19 -1.4129995101319999 1101 20 -0.6523336673641609 1101 21 -0.5646603333281774 1101 22 -0.35727131436493664 1101 23 -0.47844191895279664 1101 24 -0.6537772030158635 1101 25 -0.4181571200737544 1102 1 -0.6857540138230576 1102 2 -0.7583742751867497 1102 3 -1.2852435353993386 1102 4 -0.9144914188563149 1102 5 -1.1105755596080868 1102 6 -1.814143926381579 1102 7 -0.9897850565267738 1102 8 -0.20320608055153144 1102 9 -0.501058821122566 1102 10 -0.4798665827375146 1102 11 -0.7301157347957918 1102 12 -0.5703291880645835 1102 13 -0.6549982518085926 1102 14 -1.1407368035033087 1102 15 -0.45946600217521194 1102 16 -0.686648050069742 1102 17 -0.865770581454079 1102 18 -1.0876544798658117 1102 19 -0.654364723388035 1102 20 -0.8818221886282274 1102 21 -0.850494364611734 1102 22 -0.9053163071546706 1102 23 -1.3716381146829235 1102 24 -4.44145056502737 1102 25 -2.6184586526683695 1103 1 -0.11220014472111117 1103 2 -0.548423707207121 1103 3 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0.7872368675497867 2215 4 0.6974162885011772 2215 5 1.3498355976214107 2215 6 1.6518860245929936 2215 7 1.2280166276232372 2215 8 -0.08177857485657114 2215 9 0.2437352380251454 2215 10 4.840866970354796E-4 2215 11 -0.12375437367551001 2215 12 -0.22131136088674452 2215 13 -0.004741533085457661 2215 14 0.04155953186677408 2215 15 0.02665011825682484 2215 16 0.24095241406423895 2215 17 -0.30013039807181485 2215 18 -0.9905456445202034 2215 19 0.2512183556937089 2215 20 -4.0076836776085685 2215 21 -2.0866182193047464 2215 22 -0.9999210041811537 2215 23 -0.972636402180477 2215 24 -1.0091821317380152 2215 25 -0.7429344362077472 2216 1 -0.1221262729554411 2216 2 -0.6757303460651976 2216 3 0.4243578075081089 2216 4 -0.2496498812319743 2216 5 0.16380184754452642 2216 6 -1.0741726627883352 2216 7 -8.804733105588056 2216 8 -10.465984816079317 2216 9 -5.354566432402898 2216 10 -3.1594449044860475 2216 11 -2.037318314353172 2216 12 -1.5459760990375884 2216 13 -1.4272567591483707 2216 14 -1.446870948144003 2216 15 -2.530645253520717 2216 16 -1.4743476882082192 2216 17 -1.6490757682661155 2216 18 -1.4484508877368398 2216 19 -0.7998361414843913 2216 20 -1.6953498247618768 2216 21 -4.401181542103579 2216 22 -2.9934612111498353 2216 23 -2.7680977862456366 2216 24 -2.9655688718295323 2216 25 -2.4894796115721043 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102047.0 3 5.0 4 779.0 5 4574.0 6 5428.0 7 4579.0 8 3617.0 9 3409.0 10 3103.0 11 2939.0 12 2920.0 13 2869.0 14 2949.0 15 3170.0 16 3322.0 17 3520.0 18 3978.0 19 4919.0 20 6284.0 21 8288.0 22 10413.0 23 12229.0 24 14178.0 25 16032.0 26 18386.0 27 19938.0 28 22242.0 29 26663.0 30 31391.0 31 38585.0 32 49693.0 33 64475.0 34 87054.0 35 120350.0 36 171599.0 37 262271.0 38 285684.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8210129819137 18.156032061787407 12.38415037037402 25.638804585924873 2 15.987764075285668 20.152224914913077 38.75604712982356 25.103963879977698 3 19.013979047248096 24.492685660209947 29.02879263300062 27.464542659541337 4 12.500070276834506 16.730735714125082 37.185651719252476 33.583542289787935 5 14.463123408069482 37.23336700417116 34.63506946542043 13.668440122338938 6 32.31913683012665 36.114471160938194 17.600011239191062 13.966380769744097 7 28.939995420842212 31.504957818911922 21.73951637048909 17.815530389756777 8 25.908028520428793 35.6796284060315 20.224159525053995 18.188183548485714 9 26.485258572912695 15.753620470329247 19.415920165266385 38.34520079149167 10 15.188791124175548 28.843830003681397 33.536840685358435 22.43053818678462 11 34.108090984504145 23.218707131071714 23.342190716815896 19.331011167608246 12 22.82865078172084 25.30811559550284 30.49821094170353 21.36502268107279 13 28.114575906889517 22.323617935147173 25.31817080323246 24.24363535473086 14 22.020613111163716 21.45720493068204 26.860332228900642 29.661849729253603 15 23.86228423964273 29.19456958908074 23.278517875430396 23.664628295846136 16 23.473010989474126 28.108890270329123 25.380452152412662 23.03764658778409 17 22.666232100485935 28.12266005077119 26.42335643371016 22.787751415032712 18 22.65647059815156 27.83942526652647 27.002723915245358 22.501380220076616 19 23.87144405567142 27.248383813336496 26.69336954284554 22.186802588146538 20 23.773988330087008 26.933729307749527 26.957541156216262 22.334741205947203 21 23.680532142054396 26.718012474713422 26.55266632951225 23.048789053719936 22 23.87062061253475 26.608286540728184 26.621070509911792 22.900022336825277 23 23.446645757896103 26.72386725623312 26.518478974744408 23.31100801112637 24 23.60378850776231 27.03231217259007 26.3678342092361 22.99606511041151 25 23.54557542356988 26.60674185715399 26.71891649276203 23.128766226514095 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1852.0 1 1852.0 2 1918.5 3 1985.0 4 1985.0 5 1985.0 6 2335.0 7 2685.0 8 2685.0 9 2685.0 10 3355.0 11 4025.0 12 4025.0 13 4025.0 14 5647.5 15 7270.0 16 7270.0 17 7270.0 18 11867.5 19 16465.0 20 16465.0 21 16465.0 22 23856.5 23 31248.0 24 31248.0 25 31248.0 26 42709.5 27 54171.0 28 54171.0 29 54171.0 30 68290.0 31 82409.0 32 82409.0 33 82409.0 34 97540.5 35 112672.0 36 112672.0 37 112672.0 38 127285.0 39 141898.0 40 141898.0 41 141898.0 42 154441.0 43 166984.0 44 166984.0 45 166984.0 46 175812.5 47 184641.0 48 184641.0 49 184641.0 50 184654.5 51 184668.0 52 184668.0 53 184668.0 54 171470.5 55 158273.0 56 158273.0 57 158273.0 58 138140.0 59 118007.0 60 118007.0 61 118007.0 62 97879.5 63 77752.0 64 77752.0 65 77752.0 66 60749.0 67 43746.0 68 43746.0 69 43746.0 70 31965.0 71 20184.0 72 20184.0 73 20184.0 74 14291.5 75 8399.0 76 8399.0 77 8399.0 78 5742.5 79 3086.0 80 3086.0 81 3086.0 82 2033.5 83 981.0 84 981.0 85 981.0 86 656.0 87 331.0 88 331.0 89 331.0 90 217.5 91 104.0 92 104.0 93 104.0 94 69.0 95 34.0 96 34.0 97 34.0 98 23.0 99 12.0 100 12.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19320421214679306 2 0.10625880515379785 3 0.10808479916172829 4 0.06587624536302868 5 0.020788239474900307 6 0.020507317319834087 7 0.3095059843442083 8 0.29974393945565714 9 0.016925559842739777 10 0.0358175747709431 11 0.014818643679743126 12 0.01601256283877456 13 0.013835416137011355 14 0.015801871222474895 15 0.013765185598244798 16 0.01734694307533911 17 0.016855329303973224 18 0.013765185598244798 19 0.022122619711464855 20 0.015520949067408677 21 0.01418656883084413 22 0.01573164068370834 23 0.016644637687673557 24 0.01460795206344346 25 0.014678182602210016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1423882.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.68176672984421 #Duplication Level Percentage of deduplicated Percentage of total 1 92.08701865264787 78.90178451042988 2 5.538898279646441 9.491651806740036 3 1.1933328876576272 3.067406103339967 4 0.4413302594300752 1.5125582535723732 5 0.2462094779292834 1.0547831527306795 6 0.13049223663486942 0.6708483227642709 7 0.08736135386330184 0.5239692600043162 8 0.054786127304169305 0.3755337743765907 9 0.03861265121392054 0.2977560156718778 >10 0.17288380711766038 2.471785479789823 >50 0.005135291506649899 0.29464007378687607 >100 0.003200458407097108 0.582875896349652 >500 4.102870228307224E-4 0.21521350416664597 >1k 3.282296182645779E-4 0.5391938462770363 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2928 0.20563501750847335 No Hit GGTATCAACGCAGAGTACTTTTTTT 1857 0.1304181104894928 No Hit TATCAACGCAGAGTACTTTTTTTTT 1737 0.1219904458375062 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.023053876655509E-5 0.0 10 0.0 0.0 0.0 7.023053876655509E-5 0.0 11 0.0 0.0 0.0 7.023053876655509E-5 0.0 12 7.023053876655509E-5 0.0 0.0 7.023053876655509E-5 0.0 13 7.023053876655509E-5 0.0 0.0 7.023053876655509E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGCCGC 40 0.0053436775 14.22379 19 GTATCAA 1345 0.0 10.988023 1 TAGGACT 115 8.5423744E-7 10.765127 4 GTTCTAG 160 5.0931703E-10 10.726614 1 GGTATCA 655 0.0 10.626535 1 TATGTAC 155 3.4779077E-9 10.451583 3 CCTAGAC 120 1.463708E-6 10.323499 3 GTGTAAG 260 0.0 10.268212 1 CTAGGAC 160 5.860784E-9 10.12497 3 GTCCTAA 170 1.4624675E-9 10.095637 1 GTCCTAG 200 2.3646862E-11 10.011507 1 ATTAGAC 125 2.4689944E-6 9.91056 3 CAATACT 125 2.4902474E-6 9.903917 4 GTATTAG 140 1.0063486E-6 9.534768 1 GTCCTAC 200 2.4738256E-10 9.534768 1 GTCTAGG 150 2.5090048E-7 9.534768 1 GTGTACG 80 0.004380988 9.534768 1 CCAATAC 130 4.072448E-6 9.529384 3 TAGAACC 100 2.6883697E-4 9.522998 4 TTAAACC 150 2.5544432E-7 9.522998 4 >>END_MODULE