FastQCFastQC Report
Thu 26 May 2016
SRR1926835_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926835_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1423882
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65650.4610634870024342No Hit
GGTATCAACGCAGAGTACTTTTTTT43910.30838229572394343No Hit
TATCAACGCAGAGTACTTTTTTTTT39820.27965800536842234No Hit
ACGCAGAGTACTTTTTTTTTTTTTT20330.1427786853124065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9650.012.3432731
AGGACCG657.878113E-411.7176775
GTATCAA21050.010.5475471
TAGGACG1409.378891E-810.2006574
ACACCGT750.002600913910.1549636
GTCTTAC1201.6392654E-59.5290071
TCTTAGG1201.6426913E-59.5273272
CTAGCAC1701.6039849E-89.5209493
TTAGCCT1106.673419E-59.5206144
TAGGCTC900.00109153239.5206135
ATAGGAC1554.122594E-79.2138213
CTTATAC1252.6819065E-59.140113
TAGTACT1151.084204E-49.1066734
GTGTAAA2002.5102054E-99.0525571
ATTAGCC850.00730098238.9608943
CTAATGC850.00730098238.9608943
CTAGACA1701.627941E-78.9605774
TTAAGCA1606.5264067E-78.9255744
GTTCTAA1502.5901645E-68.893741
GAGTAAA1502.5901645E-68.893741
Produced by FastQC (version 0.11.5)