FastQCFastQC Report
Thu 26 May 2016
SRR1926834_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926834_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342208
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT7480.2185805124368805No Hit
GGTATCAACGCAGAGTACTTTTTTT5120.14961660744342622No Hit
GTCCTACAGTGGACATTTCTAAATT5030.14698662801570975No Hit
GTCCTACAGTGTGCATTTCTCATTT4200.12273237329343557No Hit
TATCAACGCAGAGTACTTTTTTTTT3910.11425799513746025No Hit
CTGTAGGACGTGGAATATGGCAAGA3860.11279689545539555No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG3470.10140031793529082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1250.015.2338034
GCTAGAC400.005175705514.2879783
TGTAGGA2850.014.0373132
TAGGACT759.371379E-713.9643184
GCCCTGA551.8881795E-413.8733361
GTAGGAC2950.013.5614713
GGAGTTA500.001473794113.3256666
CTGTAGG2950.012.9327721
GACGTGA751.441719E-512.6911127
AGGACGT1800.012.6911125
ACGTGAA751.4526349E-512.6818118
GGCGAGG905.47765E-712.64474719
TAGGACG1750.012.513484
GGACGTG1700.012.3178446
TCAGTAC701.0594315E-412.246843
TAATACA550.003009511612.1177984
TGGCGAG2400.011.8544518
ATTTCTC3050.011.81558315
GGTCTAC657.732392E-411.73897651
TTTAGGA1406.4028427E-1011.566462
Produced by FastQC (version 0.11.5)