##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926834_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 342208 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.474442444361323 31.0 29.0 31.0 22.0 34.0 2 28.58849296334393 31.0 30.0 31.0 22.0 34.0 3 28.3848653450533 31.0 28.0 33.0 16.0 34.0 4 32.00236405928558 35.0 32.0 37.0 19.0 37.0 5 31.812608121376474 35.0 32.0 37.0 19.0 37.0 6 31.558230666729006 35.0 32.0 37.0 17.0 37.0 7 31.324349518421545 35.0 32.0 37.0 17.0 37.0 8 31.12708645034599 35.0 32.0 37.0 16.0 37.0 9 33.33884654946699 37.0 33.0 39.0 17.0 39.0 10 33.55902258275668 38.0 34.0 39.0 19.0 39.0 11 33.67688949410885 38.0 34.0 39.0 22.0 39.0 12 33.60882270432018 38.0 34.0 39.0 19.0 39.0 13 33.700658663736675 38.0 34.0 39.0 22.0 39.0 14 34.742688657190946 39.0 35.0 41.0 22.0 41.0 15 34.35095029923321 39.0 34.0 41.0 17.0 41.0 16 34.40953455208528 39.0 34.0 41.0 17.0 41.0 17 34.65145759304283 39.0 34.0 41.0 19.0 41.0 18 34.70579881241818 39.0 35.0 41.0 19.0 41.0 19 34.09044499251917 39.0 34.0 41.0 11.0 41.0 20 34.38769403403778 39.0 34.0 41.0 18.0 41.0 21 34.37851832803441 39.0 34.0 41.0 18.0 41.0 22 34.400148447727695 39.0 34.0 41.0 17.0 41.0 23 34.40952578548719 39.0 35.0 41.0 16.0 41.0 24 33.10055288011969 39.0 32.0 41.0 2.0 41.0 25 33.706064732560314 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.03927348772739947 1101 2 0.0801597353143002 1101 3 -0.6154841750863937 1101 4 0.10298001994903316 1101 5 -0.12289347297168263 1101 6 -1.6021961916763239 1101 7 -0.509823957344647 1101 8 0.13359514356003643 1101 9 0.2247255951180449 1101 10 -8.840663512293244 1101 11 -3.4851995679846404 1101 12 -1.184398692298629 1101 13 -0.3607409727900617 1101 14 -0.17434640716828653 1101 15 -1.5096392212520442 1101 16 -0.030784731556565248 1101 17 0.05933376836407689 1101 18 0.06844002238647562 1101 19 -0.44185624404215673 1101 20 0.1416476411003842 1101 21 0.058242979469618206 1101 22 0.34042351501787493 1101 23 0.5027726232174246 1101 24 0.2934895048888677 1101 25 0.060623142140023845 1102 1 -0.5686043053570771 1102 2 -0.5571462267474345 1102 3 -0.9577861998639925 1102 4 -0.5578482910730216 1102 5 -0.7834021233018689 1102 6 -1.7543702742111549 1102 7 -0.8431754827498743 1102 8 -0.21185281022170344 1102 9 -0.4758419975250803 1102 10 -0.6180705183200175 1102 11 -0.9659731468589712 1102 12 -1.124614346316605 1102 13 -1.0677212707761967 1102 14 -1.2678705493471298 1102 15 -0.7541913454363254 1102 16 -1.143227329072566 1102 17 -1.3477307329262658 1102 18 -1.2262286610013078 1102 19 -0.77553912504262 1102 20 -1.2773144752093302 1102 21 -1.2766054935658318 1102 22 -1.200072037020547 1102 23 -1.8858646086769184 1102 24 -4.782683299003196 1102 25 -3.444963123365465 1103 1 -0.49578534497812043 1103 2 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-0.5799451635702013 2215 1 0.3918732289501392 2215 2 -0.14489938079789866 2215 3 0.17527876250873575 2215 4 0.33057025695707765 2215 5 0.8698529121120337 2215 6 0.9221943008220421 2215 7 0.5820472374803138 2215 8 -0.45051621216946813 2215 9 0.2331207625457452 2215 10 0.03005226831123764 2215 11 -0.04024550188331233 2215 12 -0.4513283237402419 2215 13 0.11818787043672785 2215 14 0.07347241214025502 2215 15 -0.007954072306624482 2215 16 0.24622181878046234 2215 17 -0.23030460410820552 2215 18 -0.9452484153173728 2215 19 0.07474722953905655 2215 20 -3.37499320473567 2215 21 -1.8323616356641068 2215 22 -1.0015190268390555 2215 23 -1.047336775564922 2215 24 -1.0515107669093524 2215 25 -0.9941068263313824 2216 1 -0.8818370885101778 2216 2 -1.8727267617502825 2216 3 -0.8557728247928509 2216 4 -1.2299456160587923 2216 5 -0.705990341856225 2216 6 -2.2047402229874784 2216 7 -10.32990712759905 2216 8 -11.690744386772643 2216 9 -6.49758360253362 2216 10 -4.071485430101461 2216 11 -2.716535184422998 2216 12 -2.5208223713592908 2216 13 -2.2971891136902585 2216 14 -2.5994442545264107 2216 15 -3.357755659608209 2216 16 -2.2788277843941387 2216 17 -2.7509891279177268 2216 18 -2.263849605793567 2216 19 -1.7569491990323698 2216 20 -2.3334257444182107 2216 21 -5.446796159473628 2216 22 -4.045070614140641 2216 23 -3.646493521596664 2216 24 -4.059348068496654 2216 25 -3.4738191279186807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17968.0 3 1.0 4 139.0 5 913.0 6 1220.0 7 971.0 8 753.0 9 684.0 10 594.0 11 539.0 12 610.0 13 593.0 14 633.0 15 635.0 16 594.0 17 698.0 18 778.0 19 999.0 20 1217.0 21 1734.0 22 2057.0 23 2581.0 24 2924.0 25 3412.0 26 3913.0 27 4218.0 28 4779.0 29 5685.0 30 6818.0 31 8417.0 32 11077.0 33 14152.0 34 19805.0 35 27430.0 36 40274.0 37 64200.0 38 88193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.41760011713888 18.332528003514167 12.254777070063694 24.995094809283255 2 13.98595669982446 19.87829139847864 43.17524868344061 22.96050321825629 3 19.741650452770017 24.27842064455725 29.24353028950949 26.73639861316325 4 11.075304349351823 16.94589117408812 39.342435789085826 32.63636868747423 5 12.054119462663532 39.39502881928612 35.957174423906565 12.593677294143783 6 31.370692199999418 37.74847212114304 18.65955848612122 12.221277192736329 7 27.80464074927177 32.51015420322473 21.518412369079645 18.166792678423857 8 24.232407944629053 38.40509637756328 20.391663882881776 16.970831794925893 9 26.1157517550987 16.235466730577006 20.05488884862018 37.59389266570412 10 13.501647184002291 30.813887208088886 35.246229892516496 20.438235715392327 11 34.28259961012278 23.610952802644388 23.465698695635655 18.64074889159718 12 22.2602489500434 26.101175776032644 32.15892119699436 19.4796540769296 13 28.5444151532333 22.61323715944096 25.719989010924778 23.12235867640096 14 19.839312122611457 23.247134331324492 28.45677677303203 28.45677677303203 15 22.740172024280966 31.54392230558129 23.487481039627546 22.228424630510197 16 20.967011255396073 30.105188705439474 28.381952786375447 20.545847252789006 17 20.691328148518355 29.703378313210756 28.234152557481597 21.371140980789296 18 20.1759410801964 29.34387421089549 30.01548982931962 20.464694879588496 19 22.445042659347788 28.080636251238566 28.441903492542288 21.032417596871355 20 22.157046701329524 28.327970235537904 29.47657919118055 20.03840387195202 21 22.230145810256698 27.96873949678072 27.938636356994014 21.86247833596858 22 21.512243025070582 29.230697288355532 28.590050094401935 20.667009592171944 23 21.262604120999562 28.711968434896974 28.343124360660525 21.682303083442932 24 21.63714318280789 28.27590842800235 29.352899399983052 20.73404898920671 25 21.55969335714307 28.403627574476044 28.85838956970046 21.17828949868043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 169.0 1 169.0 2 220.5 3 272.0 4 272.0 5 272.0 6 366.0 7 460.0 8 460.0 9 460.0 10 721.0 11 982.0 12 982.0 13 982.0 14 1486.5 15 1991.0 16 1991.0 17 1991.0 18 3350.0 19 4709.0 20 4709.0 21 4709.0 22 7157.0 23 9605.0 24 9605.0 25 9605.0 26 13245.5 27 16886.0 28 16886.0 29 16886.0 30 21944.0 31 27002.0 32 27002.0 33 27002.0 34 31099.5 35 35197.0 36 35197.0 37 35197.0 38 38074.0 39 40951.0 40 40951.0 41 40951.0 42 43103.5 43 45256.0 44 45256.0 45 45256.0 46 46258.0 47 47260.0 48 47260.0 49 47260.0 50 44760.5 51 42261.0 52 42261.0 53 42261.0 54 36351.0 55 30441.0 56 30441.0 57 30441.0 58 25046.5 59 19652.0 60 19652.0 61 19652.0 62 15217.5 63 10783.0 64 10783.0 65 10783.0 66 7963.0 67 5143.0 68 5143.0 69 5143.0 70 3624.5 71 2106.0 72 2106.0 73 2106.0 74 1428.5 75 751.0 76 751.0 77 751.0 78 497.5 79 244.0 80 244.0 81 244.0 82 153.5 83 63.0 84 63.0 85 63.0 86 40.5 87 18.0 88 18.0 89 18.0 90 11.5 91 5.0 92 5.0 93 5.0 94 2.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21419721339068637 2 0.11922573405648026 3 0.1236090331026744 4 0.07276276416682252 5 0.02220871516738358 6 0.019578735739667105 7 0.2834533383205536 8 0.276440059846643 9 0.018117636057602395 10 0.03185197306901066 11 0.01431877688423415 12 0.014903216757060033 13 0.014610996820647093 14 0.01577987656629886 15 0.013734337011408266 16 0.017825416121189454 17 0.016072096502711804 18 0.014026556947821208 19 0.023085374976622407 20 0.016072096502711804 21 0.014903216757060033 22 0.01577987656629886 23 0.016948756311950627 24 0.014903216757060033 25 0.014903216757060033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 342208.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.47069401789815 #Duplication Level Percentage of deduplicated Percentage of total 1 95.90462906663963 86.76558351187975 2 3.25647779140599 5.892316116847442 3 0.3733588688741167 1.013341079543363 4 0.14373393701393072 0.5201483614230069 5 0.07339480894444257 0.33200396512573793 6 0.047028467340115 0.2552818847314956 7 0.03244684794477515 0.2054842196579914 8 0.025882868571764667 0.18733128662892753 9 0.016418270946790332 0.13368351304170134 >10 0.10591998391175964 1.8715968969349457 >50 0.011325282723793753 0.7359599875408132 >100 0.008413067163974787 1.5711604026722268 >500 9.707385189201676E-4 0.5161087739726357 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 748 0.2185805124368805 No Hit GGTATCAACGCAGAGTACTTTTTTT 512 0.14961660744342622 No Hit GTCCTACAGTGGACATTTCTAAATT 503 0.14698662801570975 No Hit GTCCTACAGTGTGCATTTCTCATTT 420 0.12273237329343557 No Hit TATCAACGCAGAGTACTTTTTTTTT 391 0.11425799513746025 No Hit CTGTAGGACGTGGAATATGGCAAGA 386 0.11279689545539555 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 347 0.10140031793529082 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.9221993641294185E-4 0.0 0.0 0.0 6 0.0 2.9221993641294185E-4 0.0 0.0 0.0 7 0.0 2.9221993641294185E-4 0.0 0.0 0.0 8 0.0 2.9221993641294185E-4 0.0 0.0 0.0 9 0.0 2.9221993641294185E-4 0.0 0.0 0.0 10 0.0 2.9221993641294185E-4 0.0 0.0 0.0 11 0.0 2.9221993641294185E-4 0.0 0.0 0.0 12 0.0 2.9221993641294185E-4 0.0 0.0 0.0 13 0.0 2.9221993641294185E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 125 0.0 15.233803 4 GCTAGAC 40 0.0051757055 14.287978 3 TGTAGGA 285 0.0 14.037313 2 TAGGACT 75 9.371379E-7 13.964318 4 GCCCTGA 55 1.8881795E-4 13.873336 1 GTAGGAC 295 0.0 13.561471 3 GGAGTTA 50 0.0014737941 13.325666 6 CTGTAGG 295 0.0 12.932772 1 GACGTGA 75 1.441719E-5 12.691112 7 AGGACGT 180 0.0 12.691112 5 ACGTGAA 75 1.4526349E-5 12.681811 8 GGCGAGG 90 5.47765E-7 12.644747 19 TAGGACG 175 0.0 12.51348 4 GGACGTG 170 0.0 12.317844 6 TCAGTAC 70 1.0594315E-4 12.24684 3 TAATACA 55 0.0030095116 12.117798 4 TGGCGAG 240 0.0 11.85445 18 ATTTCTC 305 0.0 11.815583 15 GGTCTAC 65 7.732392E-4 11.7389765 1 TTTAGGA 140 6.4028427E-10 11.56646 2 >>END_MODULE