FastQCFastQC Report
Thu 26 May 2016
SRR1926834_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926834_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342208
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13320.3892369553020385No Hit
GGTATCAACGCAGAGTACTTTTTTT10470.3059542734243501No Hit
TATCAACGCAGAGTACTTTTTTTTT6710.19607957733308398No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5050.14757106788853563No Hit
CACACACACACACACACACACACAC4710.1376355900504956No Hit
GTCCTACAGTGGACATTTCTAAATT4520.1320834112586497No Hit
CTGTAGGACGTGGAATATGGCAAGA4420.1291612118945203No Hit
GTACATGGGAAGCAGTGGTATCAAC4020.11747241443800262No Hit
GTCCTACAGTGTGCATTTCTCATTT3930.11484243501028615No Hit
GTGTGTGTGTGTGTGTGTGTGTGTG3450.10081587806246495No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA250.005921036519.0560441
GGCGAGG1101.2732926E-1114.66488319
GTGTTAT400.00516597614.2920341
GCCCGGC400.005303324714.23564211
GTTTTAA551.904899E-413.8589421
TGCTACA551.904899E-413.8589422
ATTTCTC2900.013.7427515
GTCCTAA1257.2759576E-1213.7203521
TCAAACT500.001508445813.28465819
TTTCTCA3200.012.75089916
GTCCTAC2700.012.7040291
CTAGGAC604.007061E-412.6928573
TAAAGTG1501.8189894E-1212.6928565
TAGGACC751.4396886E-512.6928564
TAGGACA1051.916851E-812.6928564
CTAAAGT1301.8735591E-1012.4487644
ACGTGAA1155.340553E-912.3788198
GGACGTG1850.012.3498076
CTCAAAC550.003086612312.07696218
AGGACGT1900.012.0248115
Produced by FastQC (version 0.11.5)