##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926834_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 342208 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.760727393865718 31.0 30.0 33.0 25.0 34.0 2 30.040583504769028 31.0 30.0 33.0 26.0 34.0 3 30.059253436506452 31.0 30.0 34.0 26.0 34.0 4 33.6966815504021 35.0 35.0 37.0 28.0 37.0 5 33.68728960164578 35.0 35.0 37.0 28.0 37.0 6 33.6725266504582 37.0 35.0 37.0 29.0 37.0 7 33.642658850757435 37.0 35.0 37.0 28.0 37.0 8 33.701959042453716 37.0 35.0 37.0 29.0 37.0 9 35.206938470170186 39.0 35.0 39.0 29.0 39.0 10 35.07400469889658 39.0 35.0 39.0 29.0 39.0 11 35.14237831961848 39.0 35.0 39.0 29.0 39.0 12 35.022477557508886 39.0 35.0 39.0 28.0 39.0 13 35.0516323405648 39.0 35.0 39.0 29.0 39.0 14 36.09386688797456 40.0 36.0 41.0 28.0 41.0 15 36.09455360482514 40.0 36.0 41.0 28.0 41.0 16 36.0519830044885 40.0 36.0 41.0 28.0 41.0 17 36.049902398541235 40.0 36.0 41.0 28.0 41.0 18 36.01956412474285 40.0 36.0 41.0 27.0 41.0 19 36.109918529081725 40.0 36.0 41.0 28.0 41.0 20 36.068709673648776 40.0 36.0 41.0 28.0 41.0 21 36.034312464933606 40.0 36.0 41.0 28.0 41.0 22 35.93650937441556 40.0 36.0 41.0 27.0 41.0 23 35.885762460258086 40.0 36.0 41.0 27.0 41.0 24 35.805553932111465 40.0 36.0 41.0 27.0 41.0 25 35.68701199270619 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.02954278267451116 1101 2 0.08313420033262986 1101 3 0.2208162454586926 1101 4 0.1024238088933771 1101 5 0.0654604963750387 1101 6 0.030392027917841347 1101 7 0.06558211020282556 1101 8 0.09954002477509505 1101 9 0.03509009730125712 1101 10 0.015185023770925454 1101 11 0.2279804955558049 1101 12 0.131311647401688 1101 13 0.31301562354640566 1101 14 0.08116816184040232 1101 15 0.14197487061269243 1101 16 0.263741162104175 1101 17 0.4179082778345631 1101 18 0.4464051048876456 1101 19 0.10290110704212196 1101 20 0.12319557178081197 1101 21 0.06145111877795273 1101 22 -0.061546979519782496 1101 23 0.08055019041148626 1101 24 0.32638936636531923 1101 25 0.21797529342306632 1102 1 -0.8906775001646281 1102 2 -0.7109068565747698 1102 3 -0.4731944046999672 1102 4 -0.47464950255749017 1102 5 -0.6499752167411827 1102 6 -0.70961467716009 1102 7 -0.5506028563887355 1102 8 -0.5233149350334187 1102 9 -0.4621304139999154 1102 10 -0.4993127580957335 1102 11 -0.9845470849246212 1102 12 -0.7942736825115375 1102 13 -0.9326397198549401 1102 14 -0.9031957523657894 1102 15 -0.7000074934730378 1102 16 -0.8738610708427075 1102 17 -0.6671214662029854 1102 18 -0.4992876622044591 1102 19 -0.4768698287840465 1102 20 -0.8655734226913978 1102 21 -0.8974802789189269 1102 22 -1.0223807481634068 1102 23 -0.9998327786905818 1102 24 -0.9196675020514249 1102 25 -1.2258696789379826 1103 1 -0.9897065806929106 1103 2 -1.0025669877037977 1103 3 -0.8995205959977675 1103 4 -1.154591153277444 1103 5 -1.046185650596314 1103 6 -1.0795051938335476 1103 7 -0.9320063761678838 1103 8 -0.9448649554242934 1103 9 -1.3019208585210436 1103 10 -1.1792230382350226 1103 11 -0.8682720276733065 1103 12 -1.2083496620579197 1103 13 -1.034962711353515 1103 14 -1.0613191642301416 1103 15 -1.0494130209693537 1103 16 -0.7441829330003742 1103 17 -1.0320162179048396 1103 18 -0.755522565112507 1103 19 -0.7737688468848702 1103 20 -0.8737611442461244 1103 21 -0.9893254964739029 1103 22 -0.8734489164352013 1103 23 -1.264169134056516 1103 24 -0.8970493133887558 1103 25 -0.92822560954626 1104 1 -0.42928984918750857 1104 2 -0.3837175899259506 1104 3 -0.13205304129981243 1104 4 -0.11880017260457265 1104 5 -0.304487601982828 1104 6 0.16402908770141522 1104 7 -0.12274118056239303 1104 8 -0.37067647700781237 1104 9 -0.35951321481248044 1104 10 -0.3753264897304618 1104 11 -0.14783070932031706 1104 12 -0.4561321773857969 1104 13 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11 0.11294799223799856 1107 12 -0.2626683032622523 1107 13 -0.18602464757700687 1107 14 -0.055345772973012686 1107 15 -0.014809587777811828 1107 16 -0.057280649234122905 1107 17 -0.2148004819407987 1107 18 -0.5578274494157611 1107 19 -0.5932472310523664 1107 20 -0.47834074420992323 1107 21 -0.4284979706042975 1107 22 -0.47986880360447515 1107 23 -0.4550235100181439 1107 24 -0.34374132171529936 1107 25 -0.6249553286761937 1108 1 0.9647127469649348 1108 2 0.8971700608477917 1108 3 0.8481622351612508 1108 4 0.9342021756007242 1108 5 1.108636094471528 1108 6 1.0148086626908395 1108 7 0.8405043016948 1108 8 1.00869723767795 1108 9 0.9460670544289513 1108 10 0.9700055406649426 1108 11 0.9394102223775391 1108 12 0.8422503321310018 1108 13 0.9593642201047743 1108 14 0.958714540043907 1108 15 0.7766467750616783 1108 16 0.7599211434734627 1108 17 1.0196370348481594 1108 18 0.8750379137206252 1108 19 0.9963810790238341 1108 20 1.1496865705459811 1108 21 0.917312211635462 1108 22 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0.4495629418331859 2214 16 0.7156893391167571 2214 17 0.7082277520382831 2214 18 0.4069187949195978 2214 19 0.5728504465294648 2214 20 0.35947716581573275 2214 21 0.6045572651053277 2214 22 0.7620491946313237 2214 23 0.35519001239463677 2214 24 0.06358752704134218 2214 25 -0.015379478054342144 2215 1 0.19534645955205932 2215 2 0.050063152278706724 2215 3 -0.044506379024888076 2215 4 0.12188795870855529 2215 5 0.1531663835244217 2215 6 0.11708546670887898 2215 7 -0.023323086579082997 2215 8 -0.021841661461039052 2215 9 -0.06612416123320486 2215 10 0.10856130122255081 2215 11 -0.34999501542483813 2215 12 0.01882116033927872 2215 13 0.07762477771035492 2215 14 0.047563709785606534 2215 15 -0.015647710383177582 2215 16 -0.06979262241419804 2215 17 -0.1639743325155152 2215 18 -0.16085666397515297 2215 19 -0.15598669465389747 2215 20 -0.32268940321375084 2215 21 0.051985745870801736 2215 22 -0.1333051062864712 2215 23 -0.06433864411776113 2215 24 -0.04806363972296168 2215 25 0.04757819622802373 2216 1 -0.45371108013047845 2216 2 -0.22146462549907042 2216 3 -2.3330976488661612 2216 4 -1.8936378349422363 2216 5 -1.0332919498089126 2216 6 -0.7097498507514359 2216 7 -0.5136008643568601 2216 8 -0.8788357090800858 2216 9 -0.5935547167887592 2216 10 -0.988264095602851 2216 11 -0.9131398566946842 2216 12 -0.8943732841051641 2216 13 -1.0388434762579024 2216 14 -1.1760970045001073 2216 15 -1.1324135833990496 2216 16 -0.9236120668586381 2216 17 -1.0883294912456734 2216 18 -0.9771761084195987 2216 19 -0.933962885130093 2216 20 -1.2248719428962929 2216 21 -1.085613460478406 2216 22 -0.8842475666039249 2216 23 -1.1749537234828367 2216 24 -1.4040656238499452 2216 25 -1.3134337085338856 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8595.0 3 6.0 4 101.0 5 854.0 6 1315.0 7 929.0 8 733.0 9 610.0 10 592.0 11 526.0 12 478.0 13 443.0 14 419.0 15 445.0 16 451.0 17 481.0 18 518.0 19 640.0 20 771.0 21 1032.0 22 1368.0 23 1643.0 24 2003.0 25 2360.0 26 2817.0 27 2989.0 28 3369.0 29 4356.0 30 5575.0 31 7271.0 32 9335.0 33 12392.0 34 16838.0 35 23271.0 36 35155.0 37 60644.0 38 130883.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.696129622966595 19.25974029773031 10.07158047464846 27.972549604654635 2 14.214421529910778 20.791282726341965 41.01126224952464 23.98303349422261 3 19.530094635748814 24.306598783004144 28.858058067419933 27.305248513827106 4 11.62772348980737 17.428289227604264 38.33282681877688 32.61116046381149 5 12.267489603698541 39.076650622901376 35.478638997746856 13.17722077565322 6 31.166984313150564 38.43307432927723 17.833162292918928 12.566779064653277 7 28.271842730586105 32.156228901119135 20.73718576368084 18.834742604613915 8 24.836267034511785 38.93235293258089 19.21893311278213 17.0124469201252 9 25.95901500406226 16.306323683272254 19.5990928591886 38.135568453476886 10 13.975657154546383 31.07789418314119 34.1834334224638 20.763015239848627 11 34.25659129032069 23.77774011233394 23.139797892423598 18.82587070492177 12 22.599705442304096 26.254207967084348 31.52205676080045 19.62402982981111 13 28.680177086833915 22.573019096740257 25.521544103680544 23.225259712745284 14 19.957861638895867 23.36078268645202 28.15584764732354 28.525508027328566 15 22.78275709906398 31.484279339437098 23.460444836685728 22.27251872481319 16 21.053631281651164 30.183485434254703 27.945658621828308 20.817224662265822 17 20.73884888722648 29.62847157284458 27.93739480082289 21.69528473910604 18 20.338160863110893 29.225257448773245 29.722037147432523 20.714544540683335 19 21.981397007039682 28.063459408596653 28.738205100481295 21.216938483882373 20 22.020718575123098 28.361070118788444 29.472100056983386 20.14611124910507 21 22.149286883329435 27.519873743278 28.363046527940146 21.96779284545242 22 21.451573312137647 29.039648400972506 28.63433934916776 20.874438937722086 23 21.17250327286329 28.447026369927066 28.589337478960164 21.791132878249485 24 21.137436880493734 28.042301757995137 29.72315083224238 21.097110529268747 25 21.403167311058702 28.143128092278108 29.230557917839285 21.223146678823905 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 224.0 1 224.0 2 210.5 3 197.0 4 197.0 5 197.0 6 281.5 7 366.0 8 366.0 9 366.0 10 586.0 11 806.0 12 806.0 13 806.0 14 1249.0 15 1692.0 16 1692.0 17 1692.0 18 2837.0 19 3982.0 20 3982.0 21 3982.0 22 6403.0 23 8824.0 24 8824.0 25 8824.0 26 12668.0 27 16512.0 28 16512.0 29 16512.0 30 21331.5 31 26151.0 32 26151.0 33 26151.0 34 30248.0 35 34345.0 36 34345.0 37 34345.0 38 37475.5 39 40606.0 40 40606.0 41 40606.0 42 42826.0 43 45046.0 44 45046.0 45 45046.0 46 46730.0 47 48414.0 48 48414.0 49 48414.0 50 45862.0 51 43310.0 52 43310.0 53 43310.0 54 37378.5 55 31447.0 56 31447.0 57 31447.0 58 25814.5 59 20182.0 60 20182.0 61 20182.0 62 15740.5 63 11299.0 64 11299.0 65 11299.0 66 8396.0 67 5493.0 68 5493.0 69 5493.0 70 3853.0 71 2213.0 72 2213.0 73 2213.0 74 1490.5 75 768.0 76 768.0 77 768.0 78 507.0 79 246.0 80 246.0 81 246.0 82 154.5 83 63.0 84 63.0 85 63.0 86 41.0 87 19.0 88 19.0 89 19.0 90 10.5 91 2.0 92 2.0 93 2.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10373807742659436 2 0.10461473723583317 3 0.01577987656629886 4 0.0 5 0.005552178791845895 6 0.33839068636618663 7 0.020163175612492985 8 0.010227697774452964 9 0.009935477838040023 10 0.003798859173368244 11 0.004091079109781186 12 0.0 13 8.766598092388255E-4 14 5.844398728258837E-4 15 0.004383299046194128 16 2.9221993641294185E-4 17 0.0 18 0.0011688797456517674 19 0.0014610996820647092 20 8.766598092388255E-4 21 0.014026556947821208 22 0.0 23 0.0 24 0.0 25 0.0073054984103235465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 342208.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 88.54002432151287 #Duplication Level Percentage of deduplicated Percentage of total 1 95.08582674693669 84.18901412804932 2 3.9595765874031956 7.011620147031437 3 0.43290059796522384 1.1498708841791514 4 0.15920155313382114 0.5638283754596458 5 0.07918073879398595 0.3505332269307436 6 0.05775127825535751 0.3067979748796687 7 0.035871805239199965 0.2223263355834736 8 0.023596364022424692 0.16713781155558027 9 0.021865452472519295 0.17423709243459676 >10 0.11876693556649623 2.043571048785886 >50 0.012565632834678238 0.7465872798754769 >100 0.011573609183972109 2.0336451937056146 >500 6.613490962269777E-4 0.3443084865818893 >1k 6.613490962269777E-4 0.6965220149475464 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1332 0.3892369553020385 No Hit GGTATCAACGCAGAGTACTTTTTTT 1047 0.3059542734243501 No Hit TATCAACGCAGAGTACTTTTTTTTT 671 0.19607957733308398 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 505 0.14757106788853563 No Hit CACACACACACACACACACACACAC 471 0.1376355900504956 No Hit GTCCTACAGTGGACATTTCTAAATT 452 0.1320834112586497 No Hit CTGTAGGACGTGGAATATGGCAAGA 442 0.1291612118945203 No Hit GTACATGGGAAGCAGTGGTATCAAC 402 0.11747241443800262 No Hit GTCCTACAGTGTGCATTTCTCATTT 393 0.11484243501028615 No Hit GTGTGTGTGTGTGTGTGTGTGTGTG 345 0.10081587806246495 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCTAA 25 0.0059210365 19.056044 1 GGCGAGG 110 1.2732926E-11 14.664883 19 GTGTTAT 40 0.005165976 14.292034 1 GCCCGGC 40 0.0053033247 14.235642 11 GTTTTAA 55 1.904899E-4 13.858942 1 TGCTACA 55 1.904899E-4 13.858942 2 ATTTCTC 290 0.0 13.74275 15 GTCCTAA 125 7.2759576E-12 13.720352 1 TCAAACT 50 0.0015084458 13.284658 19 TTTCTCA 320 0.0 12.750899 16 GTCCTAC 270 0.0 12.704029 1 CTAGGAC 60 4.007061E-4 12.692857 3 TAAAGTG 150 1.8189894E-12 12.692856 5 TAGGACC 75 1.4396886E-5 12.692856 4 TAGGACA 105 1.916851E-8 12.692856 4 CTAAAGT 130 1.8735591E-10 12.448764 4 ACGTGAA 115 5.340553E-9 12.378819 8 GGACGTG 185 0.0 12.349807 6 CTCAAAC 55 0.0030866123 12.076962 18 AGGACGT 190 0.0 12.024811 5 >>END_MODULE