FastQCFastQC Report
Thu 26 May 2016
SRR1926833_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926833_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326511
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC7880.24133949545344568No Hit
GTATCAACGCAGAGTACTTTTTTTT7460.2284762228531351No Hit
CCCATGTACTCTGCGTTGATACCAC6940.21255026630036966No Hit
GGTATCAACGCAGAGTACTTTTTTT4980.15252166083225374No Hit
TATCAACGCAGAGTACTTTTTTTTT3600.1102566222883762No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACAAA400.00511885314.3114891
CCTAGAT400.00514509414.300472
TCCAATA400.00514509414.300472
TATACTC400.00520322214.2762885
CAATACT500.001471068813.3286344
AGTGTGC1153.6743586E-1013.2295718
CTGGACA604.0032883E-412.6939364
ATTTAGA550.002962358512.1430821
ACTGGGT550.003038462312.1020528
GTGTGCA1201.0139047E-811.8549919
GTCCTAC909.056837E-510.6011041
TGTGCAT1201.5424102E-610.27432510
CATATAC750.00256168210.1692223
CATGGGT951.599668E-410.0215284
AACACTG1053.995415E-59.970745
GTGGTAT2151.0913936E-119.70306315
GCTCTGG800.00434218259.5409931
GTGCATT1304.29845E-69.48253911
GTATCAA6150.09.4634241
TGGTATC2451.8189894E-129.28901816
Produced by FastQC (version 0.11.5)