##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926833_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 326511 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.58992193218605 31.0 30.0 31.0 23.0 34.0 2 28.70957486884056 31.0 30.0 33.0 23.0 34.0 3 28.499689137578827 31.0 28.0 33.0 16.0 34.0 4 32.14013616692853 35.0 33.0 37.0 22.0 37.0 5 31.917892505918637 35.0 32.0 37.0 19.0 37.0 6 31.67643969115895 35.0 32.0 37.0 17.0 37.0 7 31.448386118691253 35.0 32.0 37.0 17.0 37.0 8 31.269978653092853 35.0 32.0 37.0 17.0 37.0 9 33.501085721461145 37.0 34.0 39.0 19.0 39.0 10 33.73080539399898 38.0 34.0 39.0 22.0 39.0 11 33.887688316779524 38.0 34.0 39.0 24.0 39.0 12 33.81337535335716 38.0 35.0 39.0 23.0 39.0 13 33.89088575882589 38.0 35.0 39.0 23.0 39.0 14 34.96183895795241 39.0 35.0 41.0 24.0 41.0 15 34.52290121925448 39.0 34.0 41.0 18.0 41.0 16 34.5831166484437 39.0 34.0 41.0 18.0 41.0 17 34.834504809945145 39.0 35.0 41.0 23.0 41.0 18 34.8847205760296 39.0 35.0 41.0 24.0 41.0 19 34.243023971627295 39.0 34.0 41.0 16.0 41.0 20 34.530505863508424 39.0 34.0 41.0 18.0 41.0 21 34.534940629871585 39.0 34.0 41.0 18.0 41.0 22 34.567166190419314 39.0 35.0 41.0 18.0 41.0 23 34.56951220632689 39.0 35.0 41.0 18.0 41.0 24 33.22556361041435 39.0 33.0 41.0 2.0 41.0 25 33.823699660960884 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.1813243701360001 1101 2 -0.1898985570458258 1101 3 -1.2258781945604795 1101 4 -0.3757872646856768 1101 5 -0.2925008177015087 1101 6 -2.0987777468809306 1101 7 -0.6983626450839857 1101 8 -0.23986369900217497 1101 9 0.13423101744972854 1101 10 -9.199340119246315 1101 11 -3.456309713880465 1101 12 -1.1187447389660718 1101 13 -0.6450838930233687 1101 14 -0.33893343954075306 1101 15 -1.9054935293180648 1101 16 -0.4392083656030934 1101 17 -0.054979185098993355 1101 18 -0.026476054361538104 1101 19 -0.7122211145662476 1101 20 -0.04299753950854068 1101 21 -0.14822767783093127 1101 22 0.369508192980021 1101 23 0.14394383853569082 1101 24 0.18749317250178876 1101 25 -0.12348053009432647 1102 1 -1.300807654079403 1102 2 -1.0909503538653915 1102 3 -1.3232779190898825 1102 4 -0.9797648931342771 1102 5 -1.1427616800079505 1102 6 -2.1770322650160807 1102 7 -1.2573175263394667 1102 8 -0.7616050070701235 1102 9 -1.0729813035987306 1102 10 -0.8981881513010705 1102 11 -0.6817698749890226 1102 12 -0.9365459827575506 1102 13 -1.207695020365911 1102 14 -1.1858970022937925 1102 15 -0.5821160928641262 1102 16 -0.8483072428517318 1102 17 -0.9753097498137109 1102 18 -1.3348737337304541 1102 19 -1.125606481234307 1102 20 -0.777343714223683 1102 21 -1.0690298815956965 1102 22 -0.8551464253262466 1102 23 -1.1838661702292796 1102 24 -4.331151178264104 1102 25 -2.73667807590175 1103 1 -0.03879177307970849 1103 2 -0.13846554635860997 1103 3 0.20913135884410394 1103 4 0.08400235044859272 1103 5 -0.18527592425871475 1103 6 0.5425559503916197 1103 7 0.4175589960019508 1103 8 0.7197558445340526 1103 9 0.01900645995083039 1103 10 0.314263508947775 1103 11 0.06456290039294998 1103 12 -0.20223939535845403 1103 13 0.018283524617885405 1103 14 -0.17925930512119947 1103 15 0.007946326226054623 1103 16 -0.004849703499665736 1103 17 -0.27784856591239304 1103 18 -0.614864993408716 1103 19 -1.6018509463843387 1103 20 -0.5124707374844775 1103 21 -0.41486214987410364 1103 22 -0.2849105190950567 1103 23 -0.4466035400520809 1103 24 0.8939285977857665 1103 25 0.08029463945235449 1104 1 -0.22860527265357078 1104 2 -0.05431028005232719 1104 3 -1.551303021895258 1104 4 -0.6134556259357531 1104 5 -0.6390829577375641 1104 6 -1.594692518112172 1104 7 -0.1764174523673212 1104 8 0.18978813499616365 1104 9 0.11792250875063814 1104 10 0.4327178881666427 1104 11 -0.07911165016142974 1104 12 0.2938613412802127 1104 13 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1110 8 1.2203369032032505 1110 9 1.2153373206661442 1110 10 1.1932260336181528 1110 11 0.8531520899441389 1110 12 0.9522197867472997 1110 13 0.7439647420441275 1110 14 0.7883392877319721 1110 15 1.2904984146887912 1110 16 0.9149642001509548 1110 17 0.38482619486870817 1110 18 0.6019033749391483 1110 19 -1.9133377868827495 1110 20 0.2831266105243557 1110 21 0.2014303412721361 1110 22 0.5931617055126779 1110 23 0.5015581478938032 1110 24 0.42847105840180433 1110 25 0.49538325582363996 1111 1 -0.12121636817232684 1111 2 -0.3390590872372812 1111 3 0.28764168761919606 1111 4 0.13604385951658315 1111 5 0.042069629033886 1111 6 -0.2524939962672832 1111 7 -0.07591787336086497 1111 8 0.6994784708554604 1111 9 -0.038788128352926776 1111 10 -0.09230035488695876 1111 11 -0.2868055410773209 1111 12 0.12194254625729428 1111 13 -0.248088311285521 1111 14 -0.25893343954075476 1111 15 -1.3499510649096251 1111 16 -0.7170483263296532 1111 17 -0.5264568483292322 1111 18 -0.19725661400807581 1111 19 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-2.792342186784257 2214 25 -2.0442721903497514 2215 1 0.6036940730683895 2215 2 0.19640618143899502 2215 3 0.6032904425340071 2215 4 0.7958953230377688 2215 5 1.0716720755170641 2215 6 1.2992928101984198 2215 7 1.1092983782774084 2215 8 -0.07154817833632876 2215 9 0.25028133429452026 2215 10 -0.16485605606651887 2215 11 -0.17285184950895172 2215 12 -0.20674246903322313 2215 13 0.08543724572627553 2215 14 0.07207573477117535 2215 15 0.12384535274000541 2215 16 0.23730728747550245 2215 17 -0.4041326893078221 2215 18 -1.0649892483462153 2215 19 0.32982939143961687 2215 20 -3.867058827695434 2215 21 -2.315521728715261 2215 22 -1.1060805708523773 2215 23 -0.6956664896587625 2215 24 -0.5783821703526542 2215 25 -0.25277212188473897 2216 1 -0.6030099572032697 2216 2 -1.3149456909059865 2216 3 -0.5737702078783009 2216 4 -0.6024590124493265 2216 5 -0.5555438599414728 2216 6 -1.8575486562635781 2216 7 -10.800954004544856 2216 8 -11.771309003667426 2216 9 -6.483046049420185 2216 10 -3.7881202548313766 2216 11 -2.4794273680182144 2216 12 -2.2166307351953627 2216 13 -1.7408398399125318 2216 14 -1.9525521165835542 2216 15 -2.91117767228409 2216 16 -1.7028811964426822 2216 17 -2.1355359663577644 2216 18 -1.8390798491386988 2216 19 -1.4253985384500822 2216 20 -2.455717664663986 2216 21 -4.742134372844774 2216 22 -3.5345994727907666 2216 23 -3.511958925313639 2216 24 -3.5524763409163214 2216 25 -3.0244031503358144 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16355.0 3 0.0 4 124.0 5 719.0 6 1158.0 7 999.0 8 710.0 9 656.0 10 586.0 11 513.0 12 515.0 13 539.0 14 564.0 15 506.0 16 569.0 17 689.0 18 754.0 19 929.0 20 1237.0 21 1592.0 22 1951.0 23 2288.0 24 2669.0 25 3193.0 26 3595.0 27 3854.0 28 4339.0 29 5181.0 30 6464.0 31 7791.0 32 10242.0 33 13594.0 34 18655.0 35 26665.0 36 39114.0 37 62350.0 38 84852.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78763973896975 18.439098058234546 12.467386566641906 25.305875636153797 2 15.349822758104677 20.433414696971557 39.2701191016473 24.94664344327646 3 18.272002453235203 24.991720331186755 29.82888684452622 26.907390371051825 4 12.437746905350778 17.315324758113206 36.81674834272685 33.43017999380917 5 13.141546020990202 38.62632414118454 34.567360417598444 13.664769420226811 6 32.41983954123129 36.369122561948785 17.88236086987155 13.328677026948373 7 27.785746095609575 32.67034198928116 21.936147668115296 17.607764246993966 8 25.755840199489604 35.34645043008805 20.543805718742995 18.353903651679353 9 26.726073598019905 15.743041026316515 19.785326345003355 37.745559030660225 10 15.590618728840822 28.829179368556503 33.14572833529925 22.43447356730342 11 33.50548464269414 23.735744676940605 23.43156985452908 19.327200825836176 12 23.02491920785407 25.855324623608155 30.062029988819283 21.057726179718493 13 28.192207903595857 22.265276803518972 25.412685819658826 24.129829473226348 14 21.860593343646137 22.029376177421085 26.626334410561945 29.48369606837083 15 23.822899659681614 29.68734205520448 23.038421363785343 23.451336921328558 16 22.70329549144422 28.406229514222876 26.375572070112668 22.51490292422024 17 22.361565064496265 28.639038391437666 26.50641899446475 22.49297754960132 18 22.61648405169377 27.820168412153368 27.444013220568458 22.119334315584403 19 23.739576887494714 27.40122167149658 26.91292067712705 21.946280763881656 20 23.65916491299007 27.252900075660808 27.074009747072353 22.013925264276764 21 23.399823564007058 27.01308566947657 26.78396392864144 22.803126837874927 22 23.861728848863567 26.905593334558596 26.478282178521106 22.754395638056728 23 22.933258999681428 27.22081995736026 26.78002548582351 23.065895557134805 24 23.500203699676227 27.426859563623218 26.597664039502423 22.475272697198136 25 23.25401881983925 26.717187806312488 26.725764555969416 23.30302881787885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 486.0 1 486.0 2 530.5 3 575.0 4 575.0 5 575.0 6 649.5 7 724.0 8 724.0 9 724.0 10 861.0 11 998.0 12 998.0 13 998.0 14 1400.5 15 1803.0 16 1803.0 17 1803.0 18 2886.5 19 3970.0 20 3970.0 21 3970.0 22 5697.5 23 7425.0 24 7425.0 25 7425.0 26 10392.0 27 13359.0 28 13359.0 29 13359.0 30 16928.0 31 20497.0 32 20497.0 33 20497.0 34 23831.0 35 27165.0 36 27165.0 37 27165.0 38 30179.0 39 33193.0 40 33193.0 41 33193.0 42 35600.0 43 38007.0 44 38007.0 45 38007.0 46 40819.0 47 43631.0 48 43631.0 49 43631.0 50 42630.5 51 41630.0 52 41630.0 53 41630.0 54 37711.5 55 33793.0 56 33793.0 57 33793.0 58 29475.5 59 25158.0 60 25158.0 61 25158.0 62 20913.5 63 16669.0 64 16669.0 65 16669.0 66 13198.0 67 9727.0 68 9727.0 69 9727.0 70 7170.0 71 4613.0 72 4613.0 73 4613.0 74 3317.0 75 2021.0 76 2021.0 77 2021.0 78 1383.5 79 746.0 80 746.0 81 746.0 82 496.0 83 246.0 84 246.0 85 246.0 86 151.5 87 57.0 88 57.0 89 57.0 90 36.0 91 15.0 92 15.0 93 15.0 94 8.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22204458655297987 2 0.12342616328393223 3 0.12587631044589617 4 0.06799158374449865 5 0.02235759285292073 6 0.02205132445767524 7 0.2805418500448683 8 0.2695161878160307 9 0.018988640505220346 10 0.03552713384847677 11 0.016538493343256428 12 0.01715103013374741 13 0.01592595655276545 14 0.01715103013374741 15 0.01592595655276545 16 0.019907445690956812 17 0.017763566924238385 18 0.015313419762274473 19 0.02235759285292073 20 0.016538493343256428 21 0.014394614576538003 22 0.01561968815751996 23 0.01684476173850192 24 0.015313419762274473 25 0.014394614576538003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 326511.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.49475656776204 #Duplication Level Percentage of deduplicated Percentage of total 1 89.79460526754329 74.07584102644267 2 7.32418380669345 12.084135203814418 3 1.5227287976595798 3.7685142444494404 4 0.5533275070128587 1.825864719730897 5 0.2719780370434042 1.1218380978836697 6 0.13807003681331959 0.6834032445730045 7 0.0919809681944691 0.5311563306048854 8 0.06402334345736367 0.4225272106535549 9 0.04571637526576773 0.339422512383897 >10 0.17254010735575404 2.3583687667333773 >50 0.010050630892134102 0.5548991475035968 >100 0.00967838530289261 1.5498483659667777 >500 0.0011167367657183781 0.6841811292597544 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 788 0.24133949545344568 No Hit GTATCAACGCAGAGTACTTTTTTTT 746 0.2284762228531351 No Hit CCCATGTACTCTGCGTTGATACCAC 694 0.21255026630036966 No Hit GGTATCAACGCAGAGTACTTTTTTT 498 0.15252166083225374 No Hit TATCAACGCAGAGTACTTTTTTTTT 360 0.1102566222883762 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 3.0626839524548944E-4 13 0.0 3.0626839524548944E-4 0.0 0.0 3.0626839524548944E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACAAA 40 0.005118853 14.311489 1 CCTAGAT 40 0.005145094 14.30047 2 TCCAATA 40 0.005145094 14.30047 2 TATACTC 40 0.005203222 14.276288 5 CAATACT 50 0.0014710688 13.328634 4 AGTGTGC 115 3.6743586E-10 13.229571 8 CTGGACA 60 4.0032883E-4 12.693936 4 ATTTAGA 55 0.0029623585 12.143082 1 ACTGGGT 55 0.0030384623 12.102052 8 GTGTGCA 120 1.0139047E-8 11.854991 9 GTCCTAC 90 9.056837E-5 10.601104 1 TGTGCAT 120 1.5424102E-6 10.274325 10 CATATAC 75 0.002561682 10.169222 3 CATGGGT 95 1.599668E-4 10.021528 4 AACACTG 105 3.995415E-5 9.97074 5 GTGGTAT 215 1.0913936E-11 9.703063 15 GCTCTGG 80 0.0043421825 9.540993 1 GTGCATT 130 4.29845E-6 9.482539 11 GTATCAA 615 0.0 9.463424 1 TGGTATC 245 1.8189894E-12 9.289018 16 >>END_MODULE