FastQCFastQC Report
Thu 26 May 2016
SRR1926833_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926833_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326511
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16030.4909482375785195No Hit
GGTATCAACGCAGAGTACTTTTTTT11170.34210179748921166No Hit
TATCAACGCAGAGTACTTTTTTTTT9280.28421707078781416No Hit
GTACATGGGAAGCAGTGGTATCAAC8760.26829111423504876No Hit
CCCATGTACTCTGCGTTGATACCAC8710.2667597722588213No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4840.1482339032988169No Hit
GAGTACATGGGAAGCAGTGGTATCA3630.11117542747411267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACT508.459176E-515.2460314
CTAGAAT456.581493E-414.824813
GTCTTAG400.00512581114.3085641
GGACTGA400.00516260614.2931556
GTCCTAC1101.72804E-1013.87496951
TAAGCAG551.9032051E-413.8600285
CTCAGGG500.001449590913.3546572
CACTGTC500.001511300313.2810237
TTAGGAC801.9159652E-613.10407453
TAGGACC957.0329406E-813.0393684
TCCTACA1102.5502231E-913.0077842
GTCCTAA751.4093654E-512.7187221
TAAGACA751.4250805E-512.7050264
CGTGAAA701.1019633E-412.194999
CTAAGCA550.002990964612.1275244
TGCACTG550.002990964612.1275245
TCAATAC802.7588498E-511.9127953
GTAGGAC1459.094947E-1111.8306373
TATGGCG1251.8517312E-811.3819916
ACGTGAA752.0948004E-411.381998
Produced by FastQC (version 0.11.5)