##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926833_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 326511 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.9643197319539 31.0 30.0 33.0 26.0 34.0 2 30.255969936694324 31.0 30.0 34.0 26.0 34.0 3 30.344264052359645 31.0 30.0 34.0 26.0 34.0 4 33.96172257596222 37.0 35.0 37.0 30.0 37.0 5 33.953346135352255 37.0 35.0 37.0 30.0 37.0 6 33.8923282829675 37.0 35.0 37.0 30.0 37.0 7 33.86081939046464 37.0 35.0 37.0 30.0 37.0 8 33.88140675199304 37.0 35.0 37.0 30.0 37.0 9 35.42419091546686 39.0 35.0 39.0 30.0 39.0 10 35.32017604307359 39.0 35.0 39.0 30.0 39.0 11 35.35600025726545 39.0 35.0 39.0 30.0 39.0 12 35.27734747068246 39.0 35.0 39.0 30.0 39.0 13 35.27909626321931 39.0 35.0 39.0 30.0 39.0 14 36.39998346150666 40.0 37.0 41.0 30.0 41.0 15 36.34739411535905 40.0 37.0 41.0 29.0 41.0 16 36.329290590516095 40.0 37.0 41.0 29.0 41.0 17 36.31230494531578 40.0 37.0 41.0 29.0 41.0 18 36.28275617054249 40.0 37.0 41.0 29.0 41.0 19 36.334083690901686 40.0 37.0 41.0 29.0 41.0 20 36.29981225747371 40.0 37.0 41.0 29.0 41.0 21 36.254135389006805 40.0 37.0 41.0 29.0 41.0 22 36.16478771006184 40.0 37.0 41.0 29.0 41.0 23 36.104388519835474 40.0 37.0 41.0 29.0 41.0 24 36.02980910290924 40.0 37.0 41.0 29.0 41.0 25 35.91823858920527 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27724329681836224 1101 2 0.4169636630325826 1101 3 0.5652181978493509 1101 4 0.44942231238019303 1101 5 0.22249751313818678 1101 6 0.12987645028744055 1101 7 0.2775092613582828 1101 8 0.2520486254898415 1101 9 0.3023479549988295 1101 10 0.5524827944205413 1101 11 0.3306099612063349 1101 12 0.4359317507228866 1101 13 0.28355307003315033 1101 14 0.2879660999812046 1101 15 0.4565138329678575 1101 16 0.4628947477801262 1101 17 0.5772907145644339 1101 18 0.49777266924066765 1101 19 0.4879519653762969 1101 20 0.34116932492955243 1101 21 0.6867545456338959 1101 22 0.43756228230181904 1101 23 0.4493385314763927 1101 24 0.532116023903697 1101 25 0.41452631117063277 1102 1 -1.051301300201544 1102 2 -0.9640413872616698 1102 3 -0.7201651653961925 1102 4 -0.6711878967270337 1102 5 -0.7659243742527764 1102 6 -0.7813928013507763 1102 7 -0.6601550818947786 1102 8 -0.6608358855498508 1102 9 -0.46533433558090564 1102 10 -0.7641998718009191 1102 11 -0.838892104823131 1102 12 -0.6870491750252654 1102 13 -0.5947473598679309 1102 14 -0.7988029638361454 1102 15 -0.47843754229821656 1102 16 -0.9251222813112321 1102 17 -0.5777867093681124 1102 18 -0.6107418764415371 1102 19 -0.7142810159668258 1102 20 -0.726028805209431 1102 21 -0.9410721583186898 1102 22 -1.0648209557298216 1102 23 -0.9339829760990526 1102 24 -0.7499909087729577 1102 25 -0.838818987047155 1103 1 -0.8032740567835859 1103 2 -0.9947355831933002 1103 3 -0.561705605875428 1103 4 -0.5696970946788298 1103 5 -0.5796483285062592 1103 6 -0.5791079493178302 1103 7 -0.6996885466077885 1103 8 -0.7146383377872922 1103 9 -0.8629406418055012 1103 10 -0.4229975003702293 1103 11 -0.8971348054176431 1103 12 -1.0199441180070323 1103 13 -0.7475133418760649 1103 14 -0.6894458722194372 1103 15 -0.9418106468440115 1103 16 -0.9799842914203225 1103 17 -1.0969906804425946 1103 18 -1.2667978474830335 1103 19 -1.0794656758776142 1103 20 -1.0406309144304728 1103 21 -1.2122901139079616 1103 22 -0.8301598081090873 1103 23 -0.921144597841014 1103 24 -0.8994669017477221 1103 25 -0.9672151380073544 1104 1 0.10212329195740821 1104 2 -0.1440919746641356 1104 3 0.012608471885766193 1104 4 0.02790852954341716 1104 5 0.11362829311914169 1104 6 0.39422741133902406 1104 7 0.14152400625933836 1104 8 0.02891857465233727 1104 9 -0.1586084381715267 1104 10 -0.02677712496859641 1104 11 -0.06681778270228023 1104 12 0.07377024544672395 1104 13 0.07330860443454412 1104 14 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7411.0 3 4.0 4 121.0 5 808.0 6 1136.0 7 855.0 8 691.0 9 589.0 10 526.0 11 439.0 12 418.0 13 384.0 14 398.0 15 386.0 16 433.0 17 438.0 18 468.0 19 580.0 20 752.0 21 902.0 22 1225.0 23 1541.0 24 1756.0 25 2098.0 26 2473.0 27 2788.0 28 3096.0 29 3879.0 30 5040.0 31 6463.0 32 8544.0 33 11111.0 34 15558.0 35 21250.0 36 31843.0 37 56227.0 38 133880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57663143628944 19.260192739905378 10.585363909253417 27.57781191455177 2 15.213052023511302 21.080452932933916 37.607967106251586 26.09852793730319 3 17.9543414801496 25.24634955447258 29.571748446244182 27.227560519133633 4 13.013344113980846 17.407070512172638 36.182241945906874 33.39734342793964 5 13.601906331261812 38.17287459684953 34.31580043554034 13.909418636348322 6 31.93956548864855 36.86953902703633 17.482182207101303 13.708713277213816 7 28.223877076523905 32.33204777498338 21.448198329284693 17.995876819208018 8 26.487723296331687 35.56415251537651 19.67182484255924 18.276299345732557 9 26.88355167840329 15.467223735033128 19.543849223973954 38.10537536258963 10 15.847715891514419 28.81544893489946 32.78273786734866 22.554097306237463 11 33.780613307523616 23.42691398257844 23.319407519939233 19.473065189958714 12 23.249353767748417 25.55281953275264 30.155463265831155 21.04236343366778 13 28.723515034945756 21.909245159353887 25.40106460524462 23.966175200455734 14 22.126972751300876 21.686252530542617 26.705991528616185 29.480783189540322 15 24.00329554886509 29.071886456703044 23.350311331358863 23.57450666307301 16 22.794541021965774 28.082313450206424 26.23182280372916 22.891322724098647 17 22.333840721082726 28.144400307495353 26.88440441151699 22.637354559904935 18 22.514609136682715 26.98923150428167 28.18215786443211 22.314001494603502 19 23.468193929741815 26.899023000826926 27.794554531254782 21.83822853817647 20 23.69773481813616 26.48112756808409 27.62750070442378 22.193636909355973 21 23.364434451789183 26.31148495077528 27.631087232204663 22.69299336523087 22 23.702183700346087 26.43961900094943 26.952007595479465 22.906189703225017 23 22.86606842056905 26.569170928914886 27.429175216685554 23.13558543383051 24 23.092472183002613 26.727594032630037 27.477343656683278 22.70259012768408 25 23.11207487993727 26.226048711163386 27.412280701754387 23.24959570714496 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 209.0 1 209.0 2 192.5 3 176.0 4 176.0 5 176.0 6 324.5 7 473.0 8 473.0 9 473.0 10 651.5 11 830.0 12 830.0 13 830.0 14 1143.0 15 1456.0 16 1456.0 17 1456.0 18 2540.0 19 3624.0 20 3624.0 21 3624.0 22 5503.5 23 7383.0 24 7383.0 25 7383.0 26 10342.0 27 13301.0 28 13301.0 29 13301.0 30 17109.0 31 20917.0 32 20917.0 33 20917.0 34 24028.0 35 27139.0 36 27139.0 37 27139.0 38 30076.5 39 33014.0 40 33014.0 41 33014.0 42 35077.5 43 37141.0 44 37141.0 45 37141.0 46 40332.0 47 43523.0 48 43523.0 49 43523.0 50 42856.5 51 42190.0 52 42190.0 53 42190.0 54 38400.0 55 34610.0 56 34610.0 57 34610.0 58 30081.0 59 25552.0 60 25552.0 61 25552.0 62 21338.5 63 17125.0 64 17125.0 65 17125.0 66 13525.0 67 9925.0 68 9925.0 69 9925.0 70 7411.0 71 4897.0 72 4897.0 73 4897.0 74 3432.5 75 1968.0 76 1968.0 77 1968.0 78 1356.5 79 745.0 80 745.0 81 745.0 82 490.0 83 235.0 84 235.0 85 235.0 86 146.0 87 57.0 88 57.0 89 57.0 90 37.5 91 18.0 92 18.0 93 18.0 94 10.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11393184303132206 2 0.1133193062408311 3 0.013475809390801535 4 0.0 5 0.006125367904909788 6 0.347308360208385 7 0.019601177295711324 8 0.011944467414574087 9 0.012250735809819576 10 0.004900294323927831 11 0.0058190995096643 12 9.188051857364683E-4 13 0.001531341976227447 14 0.0 15 0.003062683952454894 16 9.188051857364683E-4 17 6.125367904909789E-4 18 9.188051857364683E-4 19 3.0626839524548944E-4 20 9.188051857364683E-4 21 0.015007151367028982 22 3.0626839524548944E-4 23 3.0626839524548944E-4 24 6.125367904909789E-4 25 0.004594025928682341 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 326511.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.73912349420554 #Duplication Level Percentage of deduplicated Percentage of total 1 87.36740765405608 68.7923310064132 2 9.109114119263893 14.344873231190622 3 1.8625792417978941 4.399735708130044 4 0.6619087040290298 2.084724447537253 5 0.3291535514812688 1.2958631069319981 6 0.17448715152855007 0.8243379223415195 7 0.12024652060500009 0.6627673944965945 8 0.0819912749336173 0.5164736897956361 9 0.0572345963539437 0.4055941755408761 >10 0.21016758771199875 2.680419875037003 >50 0.014023417221280506 0.7907238595125534 >100 0.009738484181204174 1.5474018119350288 >500 0.0011686181017445008 0.8204756881915168 >1k 7.790787344963339E-4 0.8342780829461404 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1603 0.4909482375785195 No Hit GGTATCAACGCAGAGTACTTTTTTT 1117 0.34210179748921166 No Hit TATCAACGCAGAGTACTTTTTTTTT 928 0.28421707078781416 No Hit GTACATGGGAAGCAGTGGTATCAAC 876 0.26829111423504876 No Hit CCCATGTACTCTGCGTTGATACCAC 871 0.2667597722588213 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 484 0.1482339032988169 No Hit GAGTACATGGGAAGCAGTGGTATCA 363 0.11117542747411267 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 6.125367904909789E-4 0.0 0.0 0.0 6 0.0 6.125367904909789E-4 0.0 0.0 0.0 7 0.0 6.125367904909789E-4 0.0 0.0 0.0 8 0.0 6.125367904909789E-4 0.0 0.0 0.0 9 0.0 6.125367904909789E-4 0.0 0.0 0.0 10 0.0 6.125367904909789E-4 0.0 0.0 0.0 11 0.0 6.125367904909789E-4 0.0 0.0 0.0 12 0.0 6.125367904909789E-4 0.0 0.0 0.0 13 0.0 6.125367904909789E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATACT 50 8.459176E-5 15.246031 4 CTAGAAT 45 6.581493E-4 14.82481 3 GTCTTAG 40 0.005125811 14.308564 1 GGACTGA 40 0.005162606 14.293155 6 GTCCTAC 110 1.72804E-10 13.8749695 1 TAAGCAG 55 1.9032051E-4 13.860028 5 CTCAGGG 50 0.0014495909 13.354657 2 CACTGTC 50 0.0015113003 13.281023 7 TTAGGAC 80 1.9159652E-6 13.1040745 3 TAGGACC 95 7.0329406E-8 13.039368 4 TCCTACA 110 2.5502231E-9 13.007784 2 GTCCTAA 75 1.4093654E-5 12.718722 1 TAAGACA 75 1.4250805E-5 12.705026 4 CGTGAAA 70 1.1019633E-4 12.19499 9 CTAAGCA 55 0.0029909646 12.127524 4 TGCACTG 55 0.0029909646 12.127524 5 TCAATAC 80 2.7588498E-5 11.912795 3 GTAGGAC 145 9.094947E-11 11.830637 3 TATGGCG 125 1.8517312E-8 11.38199 16 ACGTGAA 75 2.0948004E-4 11.38199 8 >>END_MODULE