FastQCFastQC Report
Thu 26 May 2016
SRR1926832_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926832_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16949
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA10085.947253525281727No Hit
GTACATGGGAAGCAGTGGTATCAAC5002.950026550238952No Hit
CCCATGTACTCTGCGTTGATACCAC4652.7435246917222256No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3131.846716620449584No Hit
GAGTACATGGGAAGCAGTGGTATCA2471.4573131158180423No Hit
CATGTACTCTGCGTTGATACCACTG1751.0325092925836332No Hit
GCGTTGATACCACTGCTTCCCATGT1590.9381084429759868No Hit
GTATCAACGCAGAGTACATGGGAAG1490.8791079119712077No Hit
GGTATCAACGCAGAGTACATGGGAA1420.8378075402678623No Hit
ACGCAGAGTACATGGGAAGCAGTGG1390.8201073809664287No Hit
ACTCTGCGTTGATACCACTGCTTCC1230.7257065313587823No Hit
TATCAACGCAGAGTACATGGGAAGC970.5723051507463568No Hit
GTACTCTGCGTTGATACCACTGCTT830.4897044073396661No Hit
AAACAAAAAAAAAAAAAAAAAAAAA700.41300371703345334No Hit
GTATCAACGCAGAGTACTTTTTTTT680.4012036108324975No Hit
GCTTCCCATGTACTCTGCGTTGATA670.3953035577320196No Hit
GCAGTGGTATCAACGCAGAGTACAT660.38940350463154166No Hit
ATACCACTGCTTCCCATGTACTCTG660.38940350463154166No Hit
GTGGTATCAACGCAGAGTACATGGG620.3658032922296301No Hit
CAGTGGTATCAACGCAGAGTACATG600.35400318602867425No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA580.3422030798277184No Hit
CATGGGAAGCAGTGGTATCAACGCA560.33040297362676263No Hit
GAACAAAAAAAAAAAAAAAAAAAAA550.32450292052628477No Hit
GGGAAGCAGTGGTATCAACGCAGAG510.3009027081243731No Hit
GTTGATACCACTGCTTCCCATGTAC500.2950026550238952No Hit
CTGCTTCCCATGTACTCTGCGTTGA490.2891026019234173No Hit
GGTATCAACGCAGAGTACTTTTTTT480.28320254882293944No Hit
GATACCACTGCTTCCCATGTACTCT470.2773024957224615No Hit
CCACTGCTTCCCATGTACTCTGCGT460.2714024426219836No Hit
ACAAAAAAAAAAAAAAAAAAAAAAA430.2537022833205499No Hit
CCATGTACTCTGCGTTGATACCACT420.24780223022007197No Hit
AAGCAGTGGTATCAACGCAGAGTAC420.24780223022007197No Hit
ACATGGGAAGCAGTGGTATCAACGC410.24190217711959408No Hit
CTTCCCATGTACTCTGCGTTGATAC380.22420201781816035No Hit
CAAAAAAAAAAAAAAAAAAAAAAAA370.21830196471768248No Hit
ATGGGAAGCAGTGGTATCAACGCAG350.20650185851672667No Hit
GCAGAGTACATGGGAAGCAGTGGTA350.20650185851672667No Hit
TACCACTGCTTCCCATGTACTCTGC340.20060180541624875No Hit
TATCAACGCAGAGTACTTTTTTTTT330.19470175231577083No Hit
TTCCCATGTACTCTGCGTTGATACC300.17700159301433713No Hit
ACACAAAAAAAAAAAAAAAAAAAAA290.1711015399138592No Hit
TGATACCACTGCTTCCCATGTACTC290.1711015399138592No Hit
AAAAAGTACTCTGCGTTGATACCAC290.1711015399138592No Hit
ACGCAGAGTACTTTTTTTTTTTTTT270.15930143371290342No Hit
GAAAACAAAAAAAAAAAAAAAAAAA220.1298011682105139No Hit
ACCATGTACTCTGCGTTGATACCAC210.12390111511003599No Hit
GCAAAAAAAAAAAAAAAAAAAAAAA210.12390111511003599No Hit
GGAAGCAGTGGTATCAACGCAGAGT190.11210100890908017No Hit
ATCAACGCAGAGTACATGGGAAGCA190.11210100890908017No Hit
GTACATGGTAAGCAGTGGTATCAAC190.11210100890908017No Hit
CTCTGCGTTGATACCACTGCTTCCC180.10620095580860228No Hit
TCAACGCAGAGTACATGGGAAGCAG170.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG2600.00269392045.1394231
Produced by FastQC (version 0.11.5)