FastQCFastQC Report
Thu 26 May 2016
SRR1926832_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926832_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16949
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC6013.545931913387221No Hit
GTACATGGGAAGCAGTGGTATCAAC5503.2450292052628478No Hit
GTATCAACGCAGAGTACTTTTTTTT5153.0385273467461205No Hit
GGTATCAACGCAGAGTACTTTTTTT4232.4957224615021536No Hit
TATCAACGCAGAGTACTTTTTTTTT3452.035518319664877No Hit
GAGTACATGGGAAGCAGTGGTATCA2591.5281137530237772No Hit
CATGTACTCTGCGTTGATACCACTG2321.3688123193108737No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1831.0797097173874564No Hit
ACGCAGAGTACATGGGAAGCAGTGG1801.0620095580860227No Hit
ACTCTGCGTTGATACCACTGCTTCC1510.8909080181721636No Hit
TATCAACGCAGAGTACATGGGAAGC1500.8850079650716856No Hit
GCGTTGATACCACTGCTTCCCATGT1500.8850079650716856No Hit
GGTATCAACGCAGAGTACATGGGAA1480.8732078588707299No Hit
GTATCAACGCAGAGTACATGGGAAG1420.8378075402678623No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1320.7788070092630833No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1230.7257065313587823No Hit
CAGTGGTATCAACGCAGAGTACATG910.5369048321434893No Hit
GTGGTATCAACGCAGAGTACATGGG870.5133046197415777No Hit
GGGAAGCAGTGGTATCAACGCAGAG870.5133046197415777No Hit
GCTTCCCATGTACTCTGCGTTGATA850.5015045135406219No Hit
GTACTCTGCGTTGATACCACTGCTT770.4543040887367986No Hit
CATGGGAAGCAGTGGTATCAACGCA770.4543040887367986No Hit
GCAGTGGTATCAACGCAGAGTACAT760.4484040356363207No Hit
GCAGAGTACTTTTTTTTTTTTTTTT700.41300371703345334No Hit
CTGCTTCCCATGTACTCTGCGTTGA690.4071036639329754No Hit
CCACTGCTTCCCATGTACTCTGCGT680.4012036108324975No Hit
GCAGAGTACATGGGAAGCAGTGGTA680.4012036108324975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA670.3953035577320196No Hit
GATACCACTGCTTCCCATGTACTCT660.38940350463154166No Hit
TACCACTGCTTCCCATGTACTCTGC620.3658032922296301No Hit
ATACCACTGCTTCCCATGTACTCTG600.35400318602867425No Hit
GTGGTATCAACGCAGAGTACTTTTT560.33040297362676263No Hit
GTTGATACCACTGCTTCCCATGTAC520.306802761224851No Hit
ACATGGGAAGCAGTGGTATCAACGC480.28320254882293944No Hit
ATGGGAAGCAGTGGTATCAACGCAG470.2773024957224615No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA470.2773024957224615No Hit
CTTCCCATGTACTCTGCGTTGATAC470.2773024957224615No Hit
ATCAACGCAGAGTACTTTTTTTTTT450.2655023895215057No Hit
AAGCAGTGGTATCAACGCAGAGTAC450.2655023895215057No Hit
CCATGTACTCTGCGTTGATACCACT430.2537022833205499No Hit
AAAAAGTACTCTGCGTTGATACCAC370.21830196471768248No Hit
ATCAACGCAGAGTACATGGGAAGCA350.20650185851672667No Hit
CAACGCAGAGTACTTTTTTTTTTTT290.1711015399138592No Hit
GTACATGGTAAGCAGTGGTATCAAC280.16520148681338132No Hit
AACGCAGAGTACTTTTTTTTTTTTT250.1475013275119476No Hit
TTCCCATGTACTCTGCGTTGATACC250.1475013275119476No Hit
CTGCGTTGATACCACTGCTTCCCAT230.1357012213109918No Hit
ACCATGTACTCTGCGTTGATACCAC220.1298011682105139No Hit
GGAAGCAGTGGTATCAACGCAGAGT190.11210100890908017No Hit
TGATACCACTGCTTCCCATGTACTC180.10620095580860228No Hit
TCAACGCAGAGTACATGGGAAGCAG180.10620095580860228No Hit
CCCCATGTACTCTGCGTTGATACCA180.10620095580860228No Hit
TAAAAAAAAAAAAAAAAAAAAAAAA170.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAG1406.1118044E-910.8385469
GGAAGCA1406.1118044E-910.8385468
AGTGGTA1657.881863E-89.196341514
CAGTGGT1605.1853567E-78.89099513
GGGAAGC1751.949793E-78.6708367
GCAGTGG1658.09272E-78.62157112
TGGTATC1802.8663635E-78.45499516
GTGGTAT1802.8663635E-78.45499515
AGCAGTG1701.2445889E-68.36799511
AAGCAGT1701.2445889E-68.36799510
TGGGAAG1951.0487747E-78.2923986
CATGGGA2209.14406E-98.2147974
ACATGGG2551.891749E-108.2063193
TACATGG2706.1299943E-107.75041252
ATGGGAA2155.0355993E-77.52101335
GTACATG2953.7089194E-97.09359841
GGTATCA2258.330135E-66.76399617
AACGCAG2601.0079109E-56.21929935
TGCGTTG1700.0062905026.1365312
TCAACGC2701.8092902E-55.9889553
Produced by FastQC (version 0.11.5)