##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926832_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16949 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.222550002950026 31.0 30.0 33.0 25.0 34.0 2 29.365390288512597 31.0 30.0 33.0 25.0 34.0 3 29.361909257183314 31.0 30.0 34.0 24.0 34.0 4 32.93191338722048 35.0 33.0 37.0 27.0 37.0 5 33.07699569296123 35.0 35.0 37.0 27.0 37.0 6 32.9358074222668 36.0 35.0 37.0 26.0 37.0 7 32.944008496076464 37.0 35.0 37.0 26.0 37.0 8 32.92778335005015 37.0 35.0 37.0 26.0 37.0 9 34.32231990087911 39.0 35.0 39.0 26.0 39.0 10 34.14962534662812 39.0 35.0 39.0 25.0 39.0 11 34.16773850964659 39.0 35.0 39.0 25.0 39.0 12 34.09369284323559 39.0 35.0 39.0 25.0 39.0 13 34.026491238421144 39.0 35.0 39.0 23.0 39.0 14 34.94861053749484 39.0 35.0 41.0 23.0 41.0 15 34.813263319369874 39.0 35.0 41.0 22.0 41.0 16 34.59360434243908 39.0 34.0 41.0 19.0 41.0 17 34.82429641866777 39.0 36.0 41.0 22.0 41.0 18 34.81444332998997 39.0 36.0 41.0 22.0 41.0 19 34.807245265207385 39.0 35.0 41.0 21.0 41.0 20 34.90176411587704 39.0 36.0 41.0 22.0 41.0 21 34.777863000767006 39.0 35.0 41.0 19.0 41.0 22 34.6888902000118 39.0 35.0 41.0 18.0 41.0 23 34.57608118473066 39.0 35.0 41.0 16.0 41.0 24 34.513540621865594 39.0 35.0 41.0 16.0 41.0 25 34.385332467992214 39.0 35.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -1.464284205107198 1101 2 -1.4783003132702461 1101 3 -1.3970002671982442 1101 4 -0.20051222179991868 1101 5 -1.791979216123174 1101 6 -2.1371413347479375 1101 7 -1.8556664414274522 1101 8 -0.6866021744806119 1101 9 -1.0455780859731476 1101 10 -1.049101399479511 1101 11 -1.51095426569443 1101 12 -0.6387548348149039 1101 13 -0.9983326487552944 1101 14 -1.5036188684028104 1101 15 -2.1297103763354173 1101 16 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1103 3 2.210692040494063 1103 4 2.0241031628154715 1103 5 2.500328476184521 1103 6 1.9428586652520607 1103 7 2.2304874047263965 1103 8 1.7657055178270795 1103 9 1.9698065294114642 1103 10 2.318590908212798 1103 11 2.3644303496901813 1103 12 2.5889374728774044 1103 13 1.2924365820139414 1103 14 2.565611900827953 1103 15 2.1087511621261257 1103 16 2.6205427992384713 1103 17 2.790405691402327 1103 18 2.6254844211563864 1103 19 3.7724423059023877 1103 20 3.2370349147725506 1103 21 1.3444641179289007 1103 22 1.6229245253988438 1103 23 2.461255360873217 1103 24 1.566221581305193 1103 25 1.7643251349842046 1104 1 0.38186964104664867 1104 2 0.7909304559605204 1104 3 1.6029997328017558 1104 4 1.145641624353928 1104 5 1.6695592454152859 1104 6 0.05516635755975585 1104 7 1.682795097034088 1104 8 1.774936287057848 1104 9 2.223652683257619 1104 10 2.104744754366642 1104 11 1.1428918881517234 1104 12 2.938168242108169 1104 13 2.0401288897062457 1104 14 2.9194580546741093 1104 15 2.7164434698184294 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1.546861900827956 2215 15 0.5337511621261228 2215 16 1.7255427992384682 2215 17 1.6429056914023263 2215 18 0.816734421156383 2215 19 1.696192305902386 2215 20 2.564534914772551 2215 21 3.060714117928903 2215 22 1.830424525398847 2215 23 1.565005360873215 2215 24 0.7412215813051901 2215 25 2.2655751349842035 2216 1 1.4637554971260514 2216 2 0.6122704063327298 2216 3 -0.3957595724091618 2216 4 0.28087735636385247 2216 5 1.4487155729587116 2216 6 1.3789876975101265 2216 7 0.9904874047263945 2216 8 1.2960280984722417 2216 9 2.0859355616695296 2216 10 1.5973005856321478 2216 11 2.4753980916256637 2216 12 1.8302277954580504 2216 13 2.0550172271752274 2216 14 2.3785151266344045 2216 15 2.3603640653519307 2216 16 2.4553815089158846 2216 17 2.7981476268861982 2216 18 2.2048392598660627 2216 19 3.2072810155798024 2216 20 2.4899381405789995 2216 21 2.7754318598643835 2216 22 2.433247106044007 2216 23 3.0922231028086955 2216 24 2.140415129692286 2216 25 3.2907767478874277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 637.0 3 0.0 4 17.0 5 64.0 6 105.0 7 75.0 8 61.0 9 53.0 10 43.0 11 46.0 12 40.0 13 33.0 14 21.0 15 35.0 16 23.0 17 26.0 18 39.0 19 51.0 20 54.0 21 65.0 22 95.0 23 106.0 24 116.0 25 141.0 26 147.0 27 175.0 28 162.0 29 239.0 30 281.0 31 344.0 32 441.0 33 645.0 34 789.0 35 1136.0 36 1683.0 37 3080.0 38 5881.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.04043270083348 18.89814979015192 10.391913459833303 22.669504049181295 2 14.922640840911775 23.95771820007086 33.305775363174675 27.813865595842685 3 21.760679726221383 29.938635827236254 28.268822279915035 20.03186216662733 4 13.157118414065728 20.91568824119417 30.479674317068856 35.44751902767125 5 11.918107262965366 39.59525635730722 32.928196353767184 15.558440025960232 6 31.338862559241704 32.138625592417064 16.759478672985782 19.76303317535545 7 21.37841505871246 36.006372809346786 21.992092995810467 20.62311913613029 8 28.509056581509235 32.43849194642752 20.396483568352114 18.655967903711137 9 27.464747182724647 15.056935512419612 20.17818160363443 37.30013570122131 10 19.157472417251757 29.169862528762756 28.52085668771019 23.151808366275297 11 28.750958758628826 26.66233996105965 18.04236238126143 26.54433889905009 12 24.851023659212935 32.32639093751844 26.479438314944836 16.343147088323796 13 31.90158711428403 21.54109386984483 24.137117234055104 22.420201781816036 14 23.45861112750015 26.432237890141014 26.29063661572954 23.8185143666293 15 32.78069502625524 22.290400613605524 24.727122544102897 20.201781816036345 16 17.806360257242314 27.759749837748537 32.78659507935571 21.647294825653432 17 22.83320549884949 28.597557378016404 28.697858280724525 19.87137884240958 18 22.91580624225618 21.2283910555195 33.91350522154699 21.942297480677325 19 21.27559148032332 27.264145377308395 35.294117647058826 16.166145495309458 20 22.113399020591185 22.709304383739454 34.314708832379495 20.86258776328987 21 19.108881676010622 25.417527294187074 35.73915609324285 19.734434936559456 22 20.762286860581742 23.606112455012095 30.503274529470765 25.128326154935394 23 17.942061478553306 23.859814738332645 35.38851849666647 22.809605286447578 24 20.172281550533956 28.933860404743644 32.12578913210219 18.768068912620215 25 20.677446004956924 21.373775522247136 31.4410480349345 26.507730437861444 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 94.0 1 94.0 2 92.0 3 90.0 4 90.0 5 90.0 6 136.0 7 182.0 8 182.0 9 182.0 10 201.0 11 220.0 12 220.0 13 220.0 14 162.0 15 104.0 16 104.0 17 104.0 18 159.5 19 215.0 20 215.0 21 215.0 22 340.5 23 466.0 24 466.0 25 466.0 26 624.5 27 783.0 28 783.0 29 783.0 30 879.5 31 976.0 32 976.0 33 976.0 34 1037.5 35 1099.0 36 1099.0 37 1099.0 38 1039.5 39 980.0 40 980.0 41 980.0 42 1106.0 43 1232.0 44 1232.0 45 1232.0 46 2472.0 47 3712.0 48 3712.0 49 3712.0 50 3302.5 51 2893.0 52 2893.0 53 2893.0 54 2168.0 55 1443.0 56 1443.0 57 1443.0 58 1125.5 59 808.0 60 808.0 61 808.0 62 750.5 63 693.0 64 693.0 65 693.0 66 576.5 67 460.0 68 460.0 69 460.0 70 375.0 71 290.0 72 290.0 73 290.0 74 204.0 75 118.0 76 118.0 77 118.0 78 88.0 79 58.0 80 58.0 81 58.0 82 42.5 83 27.0 84 27.0 85 27.0 86 16.0 87 5.0 88 5.0 89 5.0 90 3.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18880169921529294 2 0.08850079650716856 3 0.005900053100477904 4 0.0 5 0.0 6 0.4071036639329754 7 0.011800106200955809 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.023600212401911617 22 0.0 23 0.0 24 0.0 25 0.017700159301433715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 16949.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.392707534367815 #Duplication Level Percentage of deduplicated Percentage of total 1 92.27871939736346 52.03846834621512 2 5.168445281439632 5.829252463272169 3 0.8160703075957313 1.3806124255118295 4 0.3243356350700984 0.7316065844592602 5 0.2197112366603892 0.6195055755501799 6 0.15693659761456372 0.5310047790430114 7 0.11508683825068006 0.4543040887367986 8 0.0732370788867964 0.33040297362676263 9 0.10462439840970915 0.5310047790430114 >10 0.39757271395689475 5.091745825712431 >50 0.17786147729650553 7.186264676382087 >100 0.1360117179326219 15.446339017051153 >500 0.031387319522912745 9.829488465396189 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 601 3.545931913387221 No Hit GTACATGGGAAGCAGTGGTATCAAC 550 3.2450292052628478 No Hit GTATCAACGCAGAGTACTTTTTTTT 515 3.0385273467461205 No Hit GGTATCAACGCAGAGTACTTTTTTT 423 2.4957224615021536 No Hit TATCAACGCAGAGTACTTTTTTTTT 345 2.035518319664877 No Hit GAGTACATGGGAAGCAGTGGTATCA 259 1.5281137530237772 No Hit CATGTACTCTGCGTTGATACCACTG 232 1.3688123193108737 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 183 1.0797097173874564 No Hit ACGCAGAGTACATGGGAAGCAGTGG 180 1.0620095580860227 No Hit ACTCTGCGTTGATACCACTGCTTCC 151 0.8909080181721636 No Hit TATCAACGCAGAGTACATGGGAAGC 150 0.8850079650716856 No Hit GCGTTGATACCACTGCTTCCCATGT 150 0.8850079650716856 No Hit GGTATCAACGCAGAGTACATGGGAA 148 0.8732078588707299 No Hit GTATCAACGCAGAGTACATGGGAAG 142 0.8378075402678623 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 132 0.7788070092630833 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 123 0.7257065313587823 No Hit CAGTGGTATCAACGCAGAGTACATG 91 0.5369048321434893 No Hit GTGGTATCAACGCAGAGTACATGGG 87 0.5133046197415777 No Hit GGGAAGCAGTGGTATCAACGCAGAG 87 0.5133046197415777 No Hit GCTTCCCATGTACTCTGCGTTGATA 85 0.5015045135406219 No Hit GTACTCTGCGTTGATACCACTGCTT 77 0.4543040887367986 No Hit CATGGGAAGCAGTGGTATCAACGCA 77 0.4543040887367986 No Hit GCAGTGGTATCAACGCAGAGTACAT 76 0.4484040356363207 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 70 0.41300371703345334 No Hit CTGCTTCCCATGTACTCTGCGTTGA 69 0.4071036639329754 No Hit CCACTGCTTCCCATGTACTCTGCGT 68 0.4012036108324975 No Hit GCAGAGTACATGGGAAGCAGTGGTA 68 0.4012036108324975 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 67 0.3953035577320196 No Hit GATACCACTGCTTCCCATGTACTCT 66 0.38940350463154166 No Hit TACCACTGCTTCCCATGTACTCTGC 62 0.3658032922296301 No Hit ATACCACTGCTTCCCATGTACTCTG 60 0.35400318602867425 No Hit GTGGTATCAACGCAGAGTACTTTTT 56 0.33040297362676263 No Hit GTTGATACCACTGCTTCCCATGTAC 52 0.306802761224851 No Hit ACATGGGAAGCAGTGGTATCAACGC 48 0.28320254882293944 No Hit ATGGGAAGCAGTGGTATCAACGCAG 47 0.2773024957224615 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 47 0.2773024957224615 No Hit CTTCCCATGTACTCTGCGTTGATAC 47 0.2773024957224615 No Hit ATCAACGCAGAGTACTTTTTTTTTT 45 0.2655023895215057 No Hit AAGCAGTGGTATCAACGCAGAGTAC 45 0.2655023895215057 No Hit CCATGTACTCTGCGTTGATACCACT 43 0.2537022833205499 No Hit AAAAAGTACTCTGCGTTGATACCAC 37 0.21830196471768248 No Hit ATCAACGCAGAGTACATGGGAAGCA 35 0.20650185851672667 No Hit CAACGCAGAGTACTTTTTTTTTTTT 29 0.1711015399138592 No Hit GTACATGGTAAGCAGTGGTATCAAC 28 0.16520148681338132 No Hit AACGCAGAGTACTTTTTTTTTTTTT 25 0.1475013275119476 No Hit TTCCCATGTACTCTGCGTTGATACC 25 0.1475013275119476 No Hit CTGCGTTGATACCACTGCTTCCCAT 23 0.1357012213109918 No Hit ACCATGTACTCTGCGTTGATACCAC 22 0.1298011682105139 No Hit GGAAGCAGTGGTATCAACGCAGAGT 19 0.11210100890908017 No Hit TGATACCACTGCTTCCCATGTACTC 18 0.10620095580860228 No Hit TCAACGCAGAGTACATGGGAAGCAG 18 0.10620095580860228 No Hit CCCCATGTACTCTGCGTTGATACCA 18 0.10620095580860228 No Hit TAAAAAAAAAAAAAAAAAAAAAAAA 17 0.10030090270812438 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGCAG 140 6.1118044E-9 10.838546 9 GGAAGCA 140 6.1118044E-9 10.838546 8 AGTGGTA 165 7.881863E-8 9.1963415 14 CAGTGGT 160 5.1853567E-7 8.890995 13 GGGAAGC 175 1.949793E-7 8.670836 7 GCAGTGG 165 8.09272E-7 8.621571 12 TGGTATC 180 2.8663635E-7 8.454995 16 GTGGTAT 180 2.8663635E-7 8.454995 15 AGCAGTG 170 1.2445889E-6 8.367995 11 AAGCAGT 170 1.2445889E-6 8.367995 10 TGGGAAG 195 1.0487747E-7 8.292398 6 CATGGGA 220 9.14406E-9 8.214797 4 ACATGGG 255 1.891749E-10 8.206319 3 TACATGG 270 6.1299943E-10 7.7504125 2 ATGGGAA 215 5.0355993E-7 7.5210133 5 GTACATG 295 3.7089194E-9 7.0935984 1 GGTATCA 225 8.330135E-6 6.763996 17 AACGCAG 260 1.0079109E-5 6.2192993 5 TGCGTTG 170 0.006290502 6.13653 12 TCAACGC 270 1.8092902E-5 5.988955 3 >>END_MODULE