FastQCFastQC Report
Thu 26 May 2016
SRR1926831_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926831_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41262
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA5391.3062866560031021No Hit
GTACATGGGAAGCAGTGGTATCAAC4391.0639329164849014No Hit
CCCATGTACTCTGCGTTGATACCAC4050.9815326450487131No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2010.4871310164315835No Hit
GAGTACATGGGAAGCAGTGGTATCA1850.4483544181086714No Hit
GTATCAACGCAGAGTACTTTTTTTT1730.4192719693664873No Hit
GTATCAACGCAGAGTACATGGGAAG1380.33444816053511706No Hit
CATGTACTCTGCGTTGATACCACTG1330.322330473559207No Hit
GCGTTGATACCACTGCTTCCCATGT1260.3053657117929329No Hit
ACGCAGAGTACATGGGAAGCAGTGG1220.2956715622122049No Hit
TATCAACGCAGAGTACATGGGAAGC1030.2496243517037468No Hit
GGTATCAACGCAGAGTACTTTTTTT970.23508312733265474No Hit
GGTATCAACGCAGAGTACATGGGAA920.2229654403567447No Hit
ACTCTGCGTTGATACCACTGCTTCC840.20357714119528864No Hit
GTGGTATCAACGCAGAGTACATGGG610.14783578110610246No Hit
CAGTGGTATCAACGCAGAGTACATG570.13814163152537445No Hit
GCTTCCCATGTACTCTGCGTTGATA570.13814163152537445No Hit
TATCAACGCAGAGTACTTTTTTTTT520.1260239445494644No Hit
GAACAAAAAAAAAAAAAAAAAAAAA470.11390625757355435No Hit
ATACCACTGCTTCCCATGTACTCTG470.11390625757355435No Hit
GCAGTGGTATCAACGCAGAGTACAT440.10663564538800833No Hit
CATGGGAAGCAGTGGTATCAACGCA420.10178857059764432No Hit
GTACTCTGCGTTGATACCACTGCTT420.10178857059764432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAGC1409.774434E-68.8333047
CTGCGTT1000.00284167688.53033911
TCTGCGT1000.00284167688.53033910
GGAAGCA1451.5464126E-58.51833258
GAAGCAG1451.5948604E-58.4976579
TGGTATC1502.4430781E-58.21440116
CCATGTA1050.0040876698.193742
TGCGTTG1050.00441618368.12413312
TTGATAC1050.00441618368.12413316
AGTGGTA1553.684654E-57.949420514
GTGGTAT1553.684654E-57.949420515
CGTTGAT1100.00670916267.754854714
CAGTGGT1605.4768396E-57.70100113
TCAACGC2001.715538E-67.61023144
TGGGAAG1657.6722834E-57.49492456
AGCAGTG1907.260991E-67.482753811
ATCAACG2052.3096782E-67.46096663
AACGCAG2052.4870515E-67.42461636
CAACGCA2052.4870515E-67.42461635
GTTGATA1150.0099842587.41768715
Produced by FastQC (version 0.11.5)