##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926831_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41262 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.9227376278416 31.0 28.0 33.0 15.0 34.0 2 28.126193592167127 31.0 28.0 33.0 16.0 34.0 3 27.985531481750762 31.0 28.0 33.0 16.0 34.0 4 31.497843051718288 35.0 32.0 37.0 17.0 37.0 5 31.46352576220251 35.0 32.0 37.0 17.0 37.0 6 31.086908050991227 35.0 32.0 37.0 11.0 37.0 7 30.963428820706703 35.0 32.0 37.0 11.0 37.0 8 30.69521593718191 35.0 31.0 37.0 10.0 37.0 9 32.74451068779991 37.0 32.0 39.0 15.0 39.0 10 32.977703455964324 37.0 33.0 39.0 15.0 39.0 11 33.100067859047066 38.0 34.0 39.0 15.0 39.0 12 33.01228733459357 38.0 33.0 39.0 12.0 39.0 13 33.071155057922546 38.0 33.0 39.0 13.0 39.0 14 34.15946876060298 39.0 34.0 41.0 15.0 41.0 15 33.689714507294845 39.0 33.0 41.0 10.0 41.0 16 33.760942271339246 39.0 33.0 41.0 10.0 41.0 17 33.991178323881535 39.0 34.0 41.0 10.0 41.0 18 34.0266104405991 39.0 34.0 41.0 10.0 41.0 19 33.32077940962629 39.0 33.0 40.0 2.0 41.0 20 33.53390528815859 39.0 33.0 41.0 2.0 41.0 21 33.5438660268528 39.0 33.0 41.0 2.0 41.0 22 33.54565944452523 39.0 33.0 41.0 2.0 41.0 23 33.56555668653967 39.0 34.0 41.0 2.0 41.0 24 32.204304202413844 38.0 31.0 40.0 2.0 41.0 25 32.8235907130047 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.1564394202382964 1101 2 2.2679359686317504 1101 3 0.9302993848759442 1101 4 2.3690265735346614 1101 5 1.7264990271832268 1101 6 -0.17937097944798452 1101 7 1.8753257702386854 1101 8 2.319105937660108 1101 9 3.3635503742909307 1101 10 -5.891921471417721 1101 11 -0.5519339837771113 1101 12 1.4071938248689975 1101 13 2.000889984287525 1101 14 2.616940042427771 1101 15 1.962820077834273 1101 16 2.316274308698759 1101 17 2.0837427385917167 1101 18 2.50670839008221 1101 19 2.053189224074579 1101 20 3.180759751506571 1101 21 2.588784370699102 1101 22 3.7637478563135645 1101 23 3.243591834669708 1101 24 2.8695598340645105 1101 25 3.2755615857567335 1102 1 -1.9961452499934325 1102 2 -1.5955221418851835 1102 3 -0.529593663252399 1102 4 -1.6545028382300409 1102 5 -1.42893769295938 1102 6 -1.1176954001253456 1102 7 -1.3817152279787805 1102 8 -0.6263486077944371 1102 9 -0.6478578253525598 1102 10 -0.4473581915603262 1102 11 -0.5751068893029583 1102 12 -1.2341608988386703 1102 13 -1.1349388927178232 1102 14 -2.1085501536506612 1102 15 -2.252153184197809 1102 16 -2.0908558160784274 1102 17 -1.3822109155972342 1102 18 -1.1499761018963994 1102 19 -0.9906610433050957 1102 20 -0.7447304445718643 1102 21 -0.8686131337572291 1102 22 -0.49400615438162276 1102 23 -2.6455347250450885 1102 24 -4.9065542479319255 1102 25 -4.0517111415159945 1103 1 0.9512573474091646 1103 2 0.28824409188105093 1103 3 1.1781985445398107 1103 4 1.0276400189128125 1103 5 -0.7143273033489876 1103 6 0.38295395052400494 1103 7 0.6445834733199192 1103 8 1.229820223374393 1103 9 1.0281162006214615 1103 10 -0.24346208766421995 1103 11 0.6115814223853491 1103 12 0.034994945317173176 1103 13 1.0975286397497115 1103 14 0.12359270349219287 1103 15 1.1855091534645297 1103 16 0.09323509301248123 1103 17 0.4983085649222474 1103 18 0.15586805394775638 1103 19 -0.5140376666817232 1103 20 -0.4018733017147156 1103 21 0.7102829701388771 1103 22 0.6124873521118843 1103 23 0.7323873528769909 1103 24 1.8229912066135299 1103 25 0.8648473000424488 1104 1 -0.006115924480237567 1104 2 -0.5239678896857676 1104 3 -0.5383913172113424 1104 4 -1.8577286446816537 1104 5 -0.1365623263904645 1104 6 -0.32326724763267833 1104 7 -0.6509234391224759 1104 8 0.028783357014948052 1104 9 -0.309441403065172 1104 10 0.11598491694407898 1104 11 -1.2979807895962132 1104 12 -0.3711110454662361 1104 13 -1.423208687439228 1104 14 -0.4311307988119495 1104 15 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1110 13 1.8519929254639962 1110 14 1.5320748463493388 1110 15 2.413187724893099 1110 16 1.8432350930124812 1110 17 1.786254993493678 1110 18 1.7429216253763258 1110 19 -1.737251952396008 1110 20 0.9240195554281385 1110 21 0.9558186844245924 1110 22 1.7196302092547384 1110 23 1.4243516385912756 1110 24 0.5417412066135299 1110 25 1.6505615857567335 1111 1 0.7001912493281424 1111 2 1.1043421729045058 1111 3 0.524680420872432 1111 4 1.4260941766953295 1111 5 1.5743187520881143 1111 6 0.4261309228054522 1111 7 1.2701165223604356 1111 8 1.542986534675034 1111 9 0.28131449486453164 1111 10 0.2842564624423929 1111 11 0.3719758786753289 1111 12 0.2117007022468158 1111 13 -0.31078692528227236 1111 14 -0.033923287979021666 1111 15 -0.8624652601815299 1111 16 -0.7817649069875188 1111 17 -0.22237373784960823 1111 18 -0.23165859850426784 1111 19 0.565919689395038 1111 20 0.768702391249036 1111 21 0.4898671918872779 1111 22 0.7382869256726465 1111 23 0.57780313112859 1111 24 0.6345583707926323 1111 25 0.46959143650300206 1112 1 0.6613413810226092 1112 2 0.2630340078474376 1112 3 -1.4520535563005268 1112 4 -0.14567930881827706 1112 5 0.8686558899283234 1112 6 1.0216094127088766 1112 7 0.8116002800426081 1112 8 1.8926353494248112 1112 9 0.5008052762517181 1112 10 1.0296471560332634 1112 11 1.085320918183669 1112 12 0.8679781385944878 1112 13 0.10383116075811216 1112 14 1.5826263169375707 1112 15 1.448114195481331 1112 16 1.8799997988948363 1112 17 0.750409405258381 1112 18 -0.037409256976616234 1112 19 1.303189224074579 1112 20 0.6905636730751965 1112 21 0.6378039785422374 1112 22 0.4696302092547384 1112 23 -0.2907218908204925 1112 24 0.8303441477900009 1112 25 0.4961498210508495 1113 1 0.6584002045520236 1113 2 0.680002333639294 1113 3 -4.57015310381184 1113 4 0.6224202386930386 1113 5 1.1430902790685913 1113 6 1.610975928545983 1113 7 2.3371658909023374 1113 8 1.3652597838139577 1113 9 0.4374568599621256 1113 10 1.0785159343138062 1113 11 1.0332847190886483 1113 12 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14 2.6619416496280266 2209 15 2.4536590363685065 2209 16 2.349382633996086 2209 17 3.1236013049690854 2209 18 3.469099904064848 2209 19 1.2740185394072725 2209 20 2.9306793914937117 2209 21 3.3856342581950827 2209 22 2.879466274828509 2209 23 1.731216392689639 2209 24 -0.0028284655176165074 2209 25 0.9149058480518164 2210 1 0.1760472633755512 2210 2 0.05715165490626006 2210 3 0.9155935025230022 2210 4 0.7219677500052555 2210 5 1.2804205958106714 2210 6 0.5510211774147606 2210 7 1.3557179271014341 2210 8 0.8926353494248147 2210 9 0.6919817468399501 2210 10 1.2061177442685533 2210 11 0.20296797700719793 2210 12 1.7209193150650748 2210 13 0.5744193960522281 2210 14 -0.10855015365066123 2210 15 0.595173019010744 2210 16 0.21823509301248123 2210 17 0.162174111140736 2210 18 0.19788486067044175 2210 19 -0.579163717101892 2210 20 -0.4123775033953905 2210 21 -0.3180783743989366 2210 22 -0.26566390839231957 2210 23 0.01810163859127556 2210 24 -0.03730291103352812 2210 25 -0.13620312012562152 2211 1 -1.7228497954479778 2211 2 -2.1202380509760914 2211 3 -1.6901050268887623 2211 4 -1.7826278382300416 2211 5 -1.8170058747775606 2211 6 -2.7688317637617104 2211 7 -1.6902379552515079 2211 8 -0.8058940623398918 2211 9 -0.8089373708071079 2211 10 -1.4666763733785046 2211 11 -1.151811434757505 2211 12 -0.9739336261113962 2211 13 -1.1480070745360038 2211 14 -0.7804251536506612 2211 15 0.06943772489309907 2211 16 -2.4848899069875188 2211 17 -2.901245006506322 2211 18 -2.257078374623674 2211 19 -2.096626952396008 2211 20 -4.0759804445718615 2211 21 -3.6535563155754076 2211 22 -6.795994790745262 2211 23 -6.263148361408724 2211 24 -2.95825879338647 2211 25 -3.0369384142432665 2212 1 0.13192961631672873 2212 2 0.2777398902003796 2212 3 -2.1432300268887623 2212 4 -1.998620485288864 2212 5 -0.2636970512481476 2212 6 0.7421976480029961 2212 7 1.0321885153367276 2212 8 0.9220471141306952 2212 9 1.309628805663479 2212 10 -0.1027057851432076 2212 11 0.041203271124850005 2212 12 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-1.9671924790557824 2213 24 -0.8755382051511766 2213 25 -0.797967826007973 2214 1 1.8330480918759662 2214 2 1.7404573715591205 2214 3 1.5574741984633498 2214 4 2.0525394152910863 2214 5 2.335722998461879 2214 6 1.7919076728580094 2214 7 1.7670683827766602 2214 8 0.192345374279828 2214 9 1.1080960798971162 2214 10 1.7736405280299437 2214 11 2.4428188469326386 2214 12 1.1765945429026914 2214 13 1.9254964465907562 2214 14 2.6801822407155385 2214 15 2.172430682639579 2214 16 3.0773900225899453 2214 17 2.29307717659227 2214 18 2.83645155495379 2214 19 3.981938188449064 2214 20 1.1031568793718023 2214 21 1.479146149213328 2214 22 1.8639964064378347 2214 23 1.7293692442250759 2214 24 1.6099630375994423 2214 25 1.7121813040665899 2215 1 -1.4956538495020304 2215 2 -0.8613698077328493 2215 3 -0.9472840809428149 2215 4 -0.8869352706624731 2215 5 -0.8702153342370202 2215 6 0.48623580380585807 2215 7 -0.0846636309271851 2215 8 -0.951164332610162 2215 9 -1.1716907491854869 2215 10 -1.475122319324452 2215 11 -1.9448857590818278 2215 12 -1.9181903828681541 2215 13 -1.1708111285900564 2215 14 -1.5274690725695805 2215 15 -2.12144065348528 2215 16 -1.930413555636168 2215 17 -2.10014703353335 2215 18 -2.5362168881371865 2215 19 -2.2186708713149272 2215 20 -4.801487201328619 2215 21 -4.261242126386218 2215 22 -3.746586006961479 2215 23 -2.9818983614087244 2215 24 -2.687143928521607 2215 25 -1.7379519277567788 2216 1 0.8305521032861982 2216 2 -0.15524596236849675 2216 3 1.1067699731112377 2216 4 0.8366364022762909 2216 5 0.9436745049692767 2216 6 0.18449102104841586 2216 7 -7.871013271707206 2216 8 -10.807476340820905 2216 9 -5.32495794042736 2216 10 -2.1810751075557206 2216 11 -0.9939791562764952 2216 12 -0.8829526134531704 2216 13 -0.1800482137765087 2216 14 -0.08323369795446212 2216 15 -0.8190116421955054 2216 16 -0.33872693230397743 2216 17 -0.6300820318227807 2216 18 0.10387890385733556 2216 19 0.4091088070976596 2216 20 -0.4434646217870508 2216 21 -2.4504313155754076 2216 22 -0.821509031251594 2216 23 -0.4312654500163191 2216 24 -0.5456796794624204 2216 25 -0.30671689525592427 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2574.0 3 1.0 4 20.0 5 130.0 6 196.0 7 165.0 8 125.0 9 106.0 10 102.0 11 106.0 12 87.0 13 77.0 14 92.0 15 95.0 16 99.0 17 105.0 18 112.0 19 160.0 20 187.0 21 254.0 22 276.0 23 332.0 24 391.0 25 433.0 26 480.0 27 518.0 28 579.0 29 612.0 30 796.0 31 973.0 32 1275.0 33 1662.0 34 2256.0 35 3168.0 36 4637.0 37 7619.0 38 10462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.711439401302364 18.169890173972203 11.541452036155118 24.57721838857032 2 15.902140672782874 22.120285423037718 35.09295665258968 26.884617251589727 3 19.65776699029126 26.684466019417474 27.25485436893204 26.402912621359224 4 13.444889050563841 18.828664969079664 32.1741239238511 35.552322056505396 5 13.517248066713533 39.875396960073694 31.720443140772346 14.886911832440425 6 33.41656162125473 34.6601376902938 16.876757490545913 15.046543197905557 7 27.694028399144134 34.47043376774946 19.628963236724374 18.206574596382026 8 26.994357427765348 34.782079968868565 18.990174141453448 19.233388461912636 9 27.004096671757203 17.82415824303687 17.61084042372676 37.560904661479164 10 16.833555582494846 30.704327797308768 29.3781064371439 23.08401018305249 11 32.73621951810733 25.992631017598296 19.847772337227905 21.423377127066466 12 23.336484619300414 28.650037573024996 26.754417860519236 21.25905994715536 13 28.93225063628651 23.59229184341292 22.82874803054175 24.646709489758816 14 22.67713863234189 24.79334836254333 23.55222650473905 28.977286500375733 15 26.937340928372315 29.162525754454006 20.72476063507454 23.17537268209914 16 22.382487697267944 29.958061622748538 24.578798089743277 23.080652590240234 17 23.586735188596915 29.639775041210125 24.19276641132551 22.58072335886745 18 23.832840451834976 28.438454452901535 25.418141271149462 22.310563824114023 19 24.428498703061745 29.504739279047783 25.10969431044096 20.957067707449518 20 24.436418286711593 28.142725553885683 24.705483104668637 22.715373054734087 21 23.565628408677735 28.289904254029814 25.177554235850202 22.96691310144225 22 23.99767295292578 27.999709119115725 23.701944053909923 24.300673874048577 23 23.09351820429534 28.196053716003295 24.509138507781064 24.2012895719203 24 23.485638104472184 29.76851290752636 23.563204460065446 23.18264452793601 25 23.660162404557024 27.3542600896861 23.580172100351472 25.405405405405407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 608.0 1 608.0 2 518.0 3 428.0 4 428.0 5 428.0 6 377.5 7 327.0 8 327.0 9 327.0 10 293.5 11 260.0 12 260.0 13 260.0 14 247.5 15 235.0 16 235.0 17 235.0 18 297.5 19 360.0 20 360.0 21 360.0 22 490.0 23 620.0 24 620.0 25 620.0 26 823.5 27 1027.0 28 1027.0 29 1027.0 30 1347.5 31 1668.0 32 1668.0 33 1668.0 34 1937.5 35 2207.0 36 2207.0 37 2207.0 38 2649.5 39 3092.0 40 3092.0 41 3092.0 42 3541.0 43 3990.0 44 3990.0 45 3990.0 46 5126.0 47 6262.0 48 6262.0 49 6262.0 50 6110.0 51 5958.0 52 5958.0 53 5958.0 54 5370.0 55 4782.0 56 4782.0 57 4782.0 58 4151.0 59 3520.0 60 3520.0 61 3520.0 62 3103.0 63 2686.0 64 2686.0 65 2686.0 66 2173.0 67 1660.0 68 1660.0 69 1660.0 70 1282.5 71 905.0 72 905.0 73 905.0 74 663.0 75 421.0 76 421.0 77 421.0 78 292.5 79 164.0 80 164.0 81 164.0 82 109.0 83 54.0 84 54.0 85 54.0 86 38.0 87 22.0 88 22.0 89 22.0 90 14.0 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25689496388929284 2 0.14541224371092046 3 0.15025931850128446 4 0.06543550966991421 5 0.026658911347002084 6 0.02423537395182008 7 0.324754010954389 8 0.3538364596965731 9 0.02181183655663807 10 0.04120013571809413 11 0.01938829916145606 12 0.02181183655663807 13 0.016964761766274053 14 0.02181183655663807 15 0.016964761766274053 16 0.026658911347002084 17 0.02423537395182008 18 0.01938829916145606 19 0.026658911347002084 20 0.01938829916145606 21 0.016964761766274053 22 0.01938829916145606 23 0.01938829916145606 24 0.016964761766274053 25 0.016964761766274053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 41262.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.46813048325336 #Duplication Level Percentage of deduplicated Percentage of total 1 93.31139061948983 76.9521593718191 2 5.295638885623604 8.734428772235955 3 0.7376278358998472 1.8249236585720519 4 0.20571294228282588 0.6785904706509621 5 0.11167274009639121 0.4604721050845815 6 0.05289761372986952 0.26174203867965684 7 0.023510050546608676 0.13571809413019242 8 0.011755025273304338 0.07755319664582425 9 0.014693781591630423 0.10905918278319034 >10 0.18220289173621723 3.3396345305608066 >50 0.02057129422828259 1.2117686975910038 >100 0.029387563183260846 4.9076632252435655 >500 0.0029387563183260845 1.3062866560031021 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 539 1.3062866560031021 No Hit GTACATGGGAAGCAGTGGTATCAAC 439 1.0639329164849014 No Hit CCCATGTACTCTGCGTTGATACCAC 405 0.9815326450487131 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 201 0.4871310164315835 No Hit GAGTACATGGGAAGCAGTGGTATCA 185 0.4483544181086714 No Hit GTATCAACGCAGAGTACTTTTTTTT 173 0.4192719693664873 No Hit GTATCAACGCAGAGTACATGGGAAG 138 0.33444816053511706 No Hit CATGTACTCTGCGTTGATACCACTG 133 0.322330473559207 No Hit GCGTTGATACCACTGCTTCCCATGT 126 0.3053657117929329 No Hit ACGCAGAGTACATGGGAAGCAGTGG 122 0.2956715622122049 No Hit TATCAACGCAGAGTACATGGGAAGC 103 0.2496243517037468 No Hit GGTATCAACGCAGAGTACTTTTTTT 97 0.23508312733265474 No Hit GGTATCAACGCAGAGTACATGGGAA 92 0.2229654403567447 No Hit ACTCTGCGTTGATACCACTGCTTCC 84 0.20357714119528864 No Hit GTGGTATCAACGCAGAGTACATGGG 61 0.14783578110610246 No Hit CAGTGGTATCAACGCAGAGTACATG 57 0.13814163152537445 No Hit GCTTCCCATGTACTCTGCGTTGATA 57 0.13814163152537445 No Hit TATCAACGCAGAGTACTTTTTTTTT 52 0.1260239445494644 No Hit GAACAAAAAAAAAAAAAAAAAAAAA 47 0.11390625757355435 No Hit ATACCACTGCTTCCCATGTACTCTG 47 0.11390625757355435 No Hit GCAGTGGTATCAACGCAGAGTACAT 44 0.10663564538800833 No Hit CATGGGAAGCAGTGGTATCAACGCA 42 0.10178857059764432 No Hit GTACTCTGCGTTGATACCACTGCTT 42 0.10178857059764432 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAAGC 140 9.774434E-6 8.833304 7 CTGCGTT 100 0.0028416768 8.530339 11 TCTGCGT 100 0.0028416768 8.530339 10 GGAAGCA 145 1.5464126E-5 8.5183325 8 GAAGCAG 145 1.5948604E-5 8.497657 9 TGGTATC 150 2.4430781E-5 8.214401 16 CCATGTA 105 0.004087669 8.19374 2 TGCGTTG 105 0.0044161836 8.124133 12 TTGATAC 105 0.0044161836 8.124133 16 AGTGGTA 155 3.684654E-5 7.9494205 14 GTGGTAT 155 3.684654E-5 7.9494205 15 CGTTGAT 110 0.0067091626 7.7548547 14 CAGTGGT 160 5.4768396E-5 7.701001 13 TCAACGC 200 1.715538E-6 7.6102314 4 TGGGAAG 165 7.6722834E-5 7.4949245 6 AGCAGTG 190 7.260991E-6 7.4827538 11 ATCAACG 205 2.3096782E-6 7.4609666 3 AACGCAG 205 2.4870515E-6 7.4246163 6 CAACGCA 205 2.4870515E-6 7.4246163 5 GTTGATA 115 0.009984258 7.417687 15 >>END_MODULE