FastQCFastQC Report
Thu 26 May 2016
SRR1926831_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926831_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41262
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5561.3474867917211963No Hit
GTACATGGGAAGCAGTGGTATCAAC4921.1923803984295478No Hit
CCCATGTACTCTGCGTTGATACCAC4841.1729920992680916No Hit
GGTATCAACGCAGAGTACTTTTTTT3880.940332509330619No Hit
TATCAACGCAGAGTACTTTTTTTTT3480.8433910135233387No Hit
GAGTACATGGGAAGCAGTGGTATCA1930.4677427172701275No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1910.46289564247976345No Hit
ACGCAGAGTACATGGGAAGCAGTGG1740.42169550676166934No Hit
CATGTACTCTGCGTTGATACCACTG1740.42169550676166934No Hit
GCGTTGATACCACTGCTTCCCATGT1330.322330473559207No Hit
GGTATCAACGCAGAGTACATGGGAA1150.2787068004459309No Hit
GTATCAACGCAGAGTACATGGGAAG1140.2762832630507489No Hit
TATCAACGCAGAGTACATGGGAAGC1110.2690126508652029No Hit
ACTCTGCGTTGATACCACTGCTTCC1050.2544714264941108No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1050.2544714264941108No Hit
GAGTACTTTTTTTTTTTTTTTTTTT830.20115360380010663No Hit
GCAGAGTACTTTTTTTTTTTTTTTT770.1866123794290146No Hit
GTGGTATCAACGCAGAGTACATGGG700.16964761766274053No Hit
CATGGGAAGCAGTGGTATCAACGCA680.16480054287237653No Hit
ATACCACTGCTTCCCATGTACTCTG620.15025931850128446No Hit
GCTTCCCATGTACTCTGCGTTGATA610.14783578110610246No Hit
CAGTGGTATCAACGCAGAGTACATG590.14298870631573846No Hit
GATACCACTGCTTCCCATGTACTCT590.14298870631573846No Hit
GTACTCTGCGTTGATACCACTGCTT550.13329455673501042No Hit
TACCACTGCTTCCCATGTACTCTGC540.13087101933982842No Hit
ACATGGGAAGCAGTGGTATCAACGC530.12844748194464642No Hit
GTGGTATCAACGCAGAGTACTTTTT530.12844748194464642No Hit
GGGAAGCAGTGGTATCAACGCAGAG510.12360040715428239No Hit
ATCAACGCAGAGTACTTTTTTTTTT500.12117686975910039No Hit
GCAGTGGTATCAACGCAGAGTACAT470.11390625757355435No Hit
GCAGAGTACATGGGAAGCAGTGGTA470.11390625757355435No Hit
ATGGGAAGCAGTGGTATCAACGCAG460.11148272017837235No Hit
CTGCTTCCCATGTACTCTGCGTTGA440.10663564538800833No Hit
CTTCCCATGTACTCTGCGTTGATAC420.10178857059764432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG1407.590643E-910.82943712
TACTCTG1501.8353603E-910.7652897
CTGCGTT1451.31294655E-810.45600811
GTACTCT1501.9846993E-810.1815216
ACTCTGC1605.5388227E-910.0679928
ATGTACT1451.3407225E-79.8743214
TGTACTC1451.3407225E-79.8743215
CTCTGCG1553.7014615E-89.7814279
TCTGCGT1553.7014615E-89.78142710
AGTGGTA1002.6798408E-49.47575814
CGTTGAT1606.0485036E-89.47575814
GGTATCA2251.8189894E-119.3444721
CCATGTA1553.4766344E-79.2485612
CATGTAC1553.539335E-79.23726753
CCCATGT1451.4205161E-69.22731
TTGATAC1659.721043E-89.18861416
GCGTTGA1659.721043E-89.18861413
TGATACC1701.5381192E-78.91836117
TGGTATC1201.702696E-48.68611116
GTTGATA1752.398192E-78.6635515
Produced by FastQC (version 0.11.5)