##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926831_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 41262 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.78588047113567 31.0 30.0 33.0 26.0 34.0 2 29.96308952547138 31.0 30.0 33.0 26.0 34.0 3 29.970214725413214 31.0 30.0 34.0 26.0 34.0 4 33.70922398332606 35.0 35.0 37.0 28.0 37.0 5 33.79203625611943 35.0 35.0 37.0 30.0 37.0 6 33.76028791624255 37.0 35.0 37.0 30.0 37.0 7 33.787576947312296 37.0 35.0 37.0 30.0 37.0 8 33.78275410789588 37.0 35.0 37.0 29.0 37.0 9 35.29121225340507 39.0 35.0 39.0 30.0 39.0 10 35.164364306141245 39.0 35.0 39.0 29.0 39.0 11 35.225122388638454 39.0 35.0 39.0 29.0 39.0 12 35.12740536086472 39.0 35.0 39.0 29.0 39.0 13 35.106756822257765 39.0 35.0 39.0 29.0 39.0 14 36.140662110416365 40.0 36.0 41.0 28.0 41.0 15 36.11516649701905 40.0 36.0 41.0 28.0 41.0 16 36.02178760118269 40.0 36.0 41.0 28.0 41.0 17 36.0731908293345 40.0 36.0 41.0 28.0 41.0 18 36.04582909214289 40.0 36.0 41.0 28.0 41.0 19 36.105302699820655 40.0 36.0 41.0 28.0 41.0 20 36.126702535020115 40.0 37.0 41.0 29.0 41.0 21 36.07813484562067 40.0 36.0 41.0 28.0 41.0 22 35.96694294992972 40.0 36.0 41.0 28.0 41.0 23 35.906766516407345 40.0 36.0 41.0 27.0 41.0 24 35.829601085744756 40.0 36.0 41.0 27.0 41.0 25 35.72868498860937 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.16073068411723668 1101 2 0.3875639515956024 1101 3 0.5882181654630649 1101 4 0.9828816663675539 1101 5 0.6078414005280521 1101 6 0.6715536888705032 1101 7 0.5218991357492158 1101 8 0.5645158987432026 1101 9 0.9303326589794239 1101 10 1.4501773614412201 1101 11 0.5920941457624949 1101 12 0.7239880663188174 1101 13 1.1378813260763039 1101 14 1.2618627113543255 1101 15 0.9002425426141016 1101 16 0.8033012449836292 1101 17 1.188378432931117 1101 18 1.181450434519192 1101 19 1.6239105778875285 1101 20 1.6624939514180284 1101 21 1.37233851449502 1101 22 0.9289997078705596 1101 23 1.4896708294992038 1101 24 1.437602426195916 1101 25 1.520491505676766 1102 1 -1.118770207148362 1102 2 -2.523309488689602 1102 3 -1.303403937923747 1102 4 -1.3201486366627435 1102 5 -1.4723725032152615 1102 6 -1.5163250990082844 1102 7 -1.6071561227178037 1102 8 -1.4386926574065342 1102 9 -1.9538028133913414 1102 10 -2.5170240645837367 1102 11 -2.0649468524549732 1102 12 -2.446421915855872 1102 13 -2.238589262158996 1102 14 -2.1245900515690224 1102 15 -1.619900059881438 1102 16 -1.2886773646420409 1102 17 -1.3531545438959753 1102 18 -2.5864640039834867 1102 19 -2.9489949479591715 1102 20 -4.167809078885 1102 21 -3.7078753892482936 1102 22 -3.5405190087069798 1102 23 -3.726015445010603 1102 24 -3.3135562190803753 1102 25 -3.248848957781341 1103 1 -1.5574065707847247 1103 2 -2.0298029951831076 1103 3 -1.9394428989627066 1103 4 -1.3802135717276798 1103 5 -1.8343854902282501 1103 6 -1.4653510730342632 1103 7 -2.074039239600925 1103 8 -1.961744605458481 1103 9 -1.1015300861186148 1103 10 -1.0631279606876305 1103 11 -0.5315052940134137 1103 12 -0.7977206171545745 1103 13 -2.0921606907304238 1103 14 -2.8732913502703212 1103 15 -2.5195753845567666 1103 16 -3.211404637369313 1103 17 -1.936271427012862 1103 18 -3.270230237749722 1103 19 -2.4321118310760568 1103 20 -2.6827441438200665 1103 21 -2.1948883762612823 1103 22 -1.330454073642045 1103 23 -2.065301159296318 1103 24 -1.8551146606388187 1103 25 -1.218654152586538 1104 1 -0.18240657078472466 1104 2 0.032408986383710925 1104 3 0.12680134066862792 1104 4 0.5904085480879857 1104 5 1.0774808692187534 1104 6 0.8866074523113596 1104 7 0.5215828802147442 1104 8 0.625236961361793 1104 9 0.28959894613944925 1104 10 1.1108351729529247 1104 11 0.46907074285294925 1104 12 1.2195604888362084 1104 13 1.3323784797764873 1104 14 1.0287823824485685 1104 15 0.5305398228165075 1104 16 1.0708534271468153 1104 17 1.7826225822037287 1104 18 1.7677881954300076 1104 19 1.9319434684631176 1104 20 1.0119563170094281 1104 21 0.5839134670566892 1104 22 1.561827032348738 1104 23 1.725597458215205 1104 24 1.2785258923565763 1104 25 1.764640778289035 1105 1 0.9304966550217273 1105 2 0.8872476960611309 1105 3 0.5622852116363717 1105 4 0.6226666126041138 1105 5 0.948448611154241 1105 6 0.8382203555371674 1105 7 -0.5913203455917042 1105 8 -0.24573078057368747 1105 9 0.25734088162332114 1105 10 0.3366416245658286 1105 11 0.19487719446585317 1105 12 -0.45785886600249626 1105 13 -0.00633119764286505 1105 14 0.22233076954534425 1105 15 1.0144107905584434 1105 16 0.08698245940487936 1105 17 0.7826225822037287 1105 18 1.0419817438171037 1105 19 0.6577499200760215 1105 20 1.0119563170094281 1105 21 0.6484295960889526 1105 22 0.7715044517035778 1105 23 1.741726490473269 1105 24 1.8430420213888326 1105 25 1.3614149718374264 1106 1 -0.38081926919742415 1106 2 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14 1.0250602980813284 1107 15 0.8905894506080685 1107 16 1.7309030549383806 1107 17 1.576915386173951 1107 18 1.6918576743381948 1107 19 1.5503057513415257 1107 20 1.7650580539821306 1107 21 2.019397338024433 1107 22 0.7461942780063069 1107 23 0.41244609345093863 1107 24 0.005324899800740468 1107 25 0.5343678254354387 1108 1 -0.05163734001549258 1108 2 -0.456176621556736 1108 3 0.33995270543289635 1108 4 -0.17609269260680094 1108 5 0.05490022405746231 1108 6 -0.6002411829243712 1108 7 -0.552610668172349 1108 8 -0.6526786713925432 1108 9 -0.13422239381092282 1108 10 -0.483457631017302 1108 11 -1.1152965028046182 1108 12 -0.9653030347369906 1108 13 -1.6385892621589946 1108 14 -0.6364781634571344 1108 15 -0.44787208785346877 1108 16 -0.9614046373693128 1108 17 -1.0846230753645116 1108 18 -0.5081423256618081 1108 19 -0.018925017889245055 1108 20 -0.3272496383255614 1108 21 -0.04214112351402832 1108 22 -0.5614980296859997 1108 23 0.15090763191247447 1108 24 0.5284018228776688 1108 25 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25 0.0899474570714105 2215 1 -0.601325489703644 2215 2 -1.0626215280016424 2215 3 -0.9215857561055643 2215 4 -0.7513525678666753 2215 5 -0.299153829996591 2215 6 -0.21583369851688872 2215 7 0.19333527777359194 2215 8 -1.1214936402075182 2215 9 -1.213016572605099 2215 10 -0.8555990031586731 2215 11 -1.9219493094574247 2215 12 -1.1461762156101685 2215 13 -1.4439946675643967 2215 14 -1.6329438599228325 2215 15 -2.1368533768347575 2215 16 -1.3803235562882321 2215 17 -1.6428351335765683 2215 18 -1.4854812030006812 2215 19 -1.2467836457478683 2215 20 -1.2609294720053938 2215 21 -0.7914134727863811 2215 22 -2.1417475099354775 2215 23 -1.3206100396051994 2215 24 -1.5732613787855385 2215 25 -2.3417236506560357 2216 1 0.31759342921527534 2216 2 0.2776111096993503 2216 3 -0.9469022118017669 2216 4 -1.5951774053623993 2216 5 -0.26408711771468774 2216 6 0.08505579857514078 2216 7 0.3992880660048641 2216 8 -0.00808276097384919 2216 9 -0.9227326177641828 2216 10 -0.9089688286803934 2216 11 0.32676776204445446 2216 12 0.4124963810371085 2216 13 0.37913225682834906 2216 14 -0.8113564497278247 2216 15 -0.2295663429654411 2216 16 -0.12596159939462837 2216 17 -0.45073797312497277 2216 18 -0.2064870189432071 2216 19 -0.2946796430109586 2216 20 0.29962475310579606 2216 21 -0.10718494045657678 2216 22 0.020585709359764337 2216 23 -0.3336103817194669 2216 24 -0.09652514888475139 2216 25 -0.31743353775832617 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1018.0 3 1.0 4 14.0 5 123.0 6 145.0 7 118.0 8 101.0 9 82.0 10 53.0 11 70.0 12 48.0 13 45.0 14 52.0 15 57.0 16 59.0 17 63.0 18 69.0 19 88.0 20 89.0 21 148.0 22 166.0 23 215.0 24 216.0 25 290.0 26 316.0 27 339.0 28 402.0 29 494.0 30 602.0 31 835.0 32 1121.0 33 1472.0 34 1965.0 35 2859.0 36 4079.0 37 7356.0 38 16092.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.914493632504545 18.39660400242571 10.2874469375379 26.40145542753184 2 16.405377857593553 21.02121050332476 34.88326942678251 27.69014221229918 3 20.060598715307236 24.96424675796873 28.626833111138044 26.34832141558599 4 14.008046144152003 17.110174009984974 32.300906402985795 36.58087344287722 5 14.474417702804237 37.422138200150265 32.615914103589525 15.487529993455972 6 33.53754892665257 33.73447110592468 16.39073250188413 16.337247465538617 7 27.762829369985216 32.78064625602987 19.71492982328558 19.741594550699343 8 28.68049832759707 32.447040573949295 18.515197052692812 20.35726404576082 9 27.65524261961317 14.678365407920888 18.680013573125212 38.98637839934073 10 17.838532199035363 27.894519983518745 30.43941927821809 23.827528539227806 11 33.931168201648084 23.133785748909354 20.43868153174988 22.49636451769268 12 24.894576123309584 26.53288740245262 27.521690659686882 21.050845814550918 13 30.669865736028306 20.84726867335563 23.857302118171685 24.62556347244438 14 23.566477630749844 22.1850613154961 25.185400610731424 29.063060443022636 15 28.052445349231736 26.02879162425476 22.75216906596869 23.166593960544812 16 23.06722892734235 26.561969851194807 26.48684019194416 23.883961029518687 17 23.53012456982211 27.23086617226504 26.31719257428142 22.92181668363143 18 24.08026755852843 24.162667829964615 28.41839949590422 23.338665115602733 19 23.67068973874267 25.49318985991954 28.968542484610538 21.867577916727253 20 24.2184091900538 24.005137899277788 28.714071058116424 23.062381852551987 21 23.785054657391473 24.74973943815595 28.87752381414063 22.587682090311947 22 24.109350007270614 23.934855314817508 26.852794338616643 25.103000339295235 23 23.09388783868935 24.400174494692454 28.35538752362949 24.150550142988706 24 23.22718239542436 26.082109446948763 27.853715282826812 22.836992874800057 25 23.320972393901936 23.400954943163914 27.480064955524856 25.798007707409294 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 66.0 1 66.0 2 57.0 3 48.0 4 48.0 5 48.0 6 104.0 7 160.0 8 160.0 9 160.0 10 160.5 11 161.0 12 161.0 13 161.0 14 156.0 15 151.0 16 151.0 17 151.0 18 245.5 19 340.0 20 340.0 21 340.0 22 540.0 23 740.0 24 740.0 25 740.0 26 1002.0 27 1264.0 28 1264.0 29 1264.0 30 1630.0 31 1996.0 32 1996.0 33 1996.0 34 2231.0 35 2466.0 36 2466.0 37 2466.0 38 2756.0 39 3046.0 40 3046.0 41 3046.0 42 3425.0 43 3804.0 44 3804.0 45 3804.0 46 5094.5 47 6385.0 48 6385.0 49 6385.0 50 6265.5 51 6146.0 52 6146.0 53 6146.0 54 5536.0 55 4926.0 56 4926.0 57 4926.0 58 4258.5 59 3591.0 60 3591.0 61 3591.0 62 3144.5 63 2698.0 64 2698.0 65 2698.0 66 2227.5 67 1757.0 68 1757.0 69 1757.0 70 1331.0 71 905.0 72 905.0 73 905.0 74 653.5 75 402.0 76 402.0 77 402.0 78 278.0 79 154.0 80 154.0 81 154.0 82 97.0 83 40.0 84 40.0 85 40.0 86 25.5 87 11.0 88 11.0 89 11.0 90 8.0 91 5.0 92 5.0 93 5.0 94 2.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08967088362173428 2 0.13571809413019242 3 0.016964761766274053 4 0.0 5 0.007270612185546023 6 0.31263632397847896 7 0.02181183655663807 8 0.00969414958072803 9 0.00969414958072803 10 0.007270612185546023 11 0.004847074790364015 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.01211768697591004 22 0.0 23 0.0 24 0.0 25 0.007270612185546023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 41262.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.79647132955262 #Duplication Level Percentage of deduplicated Percentage of total 1 92.00319872051179 72.49527410207939 2 6.415895180389383 10.110998012699337 3 0.8519669055454742 2.0139595753962483 4 0.2614338879832682 0.8240027143618825 5 0.11380063359271676 0.4483544181086714 6 0.04921108479685049 0.23265958993747274 7 0.03383262079783471 0.1866123794290146 8 0.012302771199212623 0.07755319664582425 9 0.03690831359763787 0.26174203867965684 >10 0.13533048319133884 2.4720081430856475 >50 0.03998400639744103 1.950947603121516 >100 0.04305969919724418 7.578401434734137 >500 0.0030756927998031557 1.3474867917211963 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 556 1.3474867917211963 No Hit GTACATGGGAAGCAGTGGTATCAAC 492 1.1923803984295478 No Hit CCCATGTACTCTGCGTTGATACCAC 484 1.1729920992680916 No Hit GGTATCAACGCAGAGTACTTTTTTT 388 0.940332509330619 No Hit TATCAACGCAGAGTACTTTTTTTTT 348 0.8433910135233387 No Hit GAGTACATGGGAAGCAGTGGTATCA 193 0.4677427172701275 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 191 0.46289564247976345 No Hit ACGCAGAGTACATGGGAAGCAGTGG 174 0.42169550676166934 No Hit CATGTACTCTGCGTTGATACCACTG 174 0.42169550676166934 No Hit GCGTTGATACCACTGCTTCCCATGT 133 0.322330473559207 No Hit GGTATCAACGCAGAGTACATGGGAA 115 0.2787068004459309 No Hit GTATCAACGCAGAGTACATGGGAAG 114 0.2762832630507489 No Hit TATCAACGCAGAGTACATGGGAAGC 111 0.2690126508652029 No Hit ACTCTGCGTTGATACCACTGCTTCC 105 0.2544714264941108 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 105 0.2544714264941108 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 83 0.20115360380010663 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 77 0.1866123794290146 No Hit GTGGTATCAACGCAGAGTACATGGG 70 0.16964761766274053 No Hit CATGGGAAGCAGTGGTATCAACGCA 68 0.16480054287237653 No Hit ATACCACTGCTTCCCATGTACTCTG 62 0.15025931850128446 No Hit GCTTCCCATGTACTCTGCGTTGATA 61 0.14783578110610246 No Hit CAGTGGTATCAACGCAGAGTACATG 59 0.14298870631573846 No Hit GATACCACTGCTTCCCATGTACTCT 59 0.14298870631573846 No Hit GTACTCTGCGTTGATACCACTGCTT 55 0.13329455673501042 No Hit TACCACTGCTTCCCATGTACTCTGC 54 0.13087101933982842 No Hit ACATGGGAAGCAGTGGTATCAACGC 53 0.12844748194464642 No Hit GTGGTATCAACGCAGAGTACTTTTT 53 0.12844748194464642 No Hit GGGAAGCAGTGGTATCAACGCAGAG 51 0.12360040715428239 No Hit ATCAACGCAGAGTACTTTTTTTTTT 50 0.12117686975910039 No Hit GCAGTGGTATCAACGCAGAGTACAT 47 0.11390625757355435 No Hit GCAGAGTACATGGGAAGCAGTGGTA 47 0.11390625757355435 No Hit ATGGGAAGCAGTGGTATCAACGCAG 46 0.11148272017837235 No Hit CTGCTTCCCATGTACTCTGCGTTGA 44 0.10663564538800833 No Hit CTTCCCATGTACTCTGCGTTGATAC 42 0.10178857059764432 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0024235373951820077 2 0.0 0.0 0.0 0.0 0.0024235373951820077 3 0.0 0.0 0.0 0.0 0.0024235373951820077 4 0.0 0.0 0.0 0.0 0.0024235373951820077 5 0.0 0.0 0.0 0.0 0.0024235373951820077 6 0.0 0.0 0.0 0.0 0.0024235373951820077 7 0.0 0.0 0.0 0.0 0.0024235373951820077 8 0.0 0.0 0.0 0.0 0.0024235373951820077 9 0.0 0.0 0.0 0.0 0.0024235373951820077 10 0.0 0.0 0.0 0.0 0.0024235373951820077 11 0.0 0.0 0.0 0.0 0.0024235373951820077 12 0.0 0.0 0.0 0.0 0.0024235373951820077 13 0.0 0.0 0.0 0.0 0.0024235373951820077 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTTG 140 7.590643E-9 10.829437 12 TACTCTG 150 1.8353603E-9 10.765289 7 CTGCGTT 145 1.31294655E-8 10.456008 11 GTACTCT 150 1.9846993E-8 10.181521 6 ACTCTGC 160 5.5388227E-9 10.067992 8 ATGTACT 145 1.3407225E-7 9.874321 4 TGTACTC 145 1.3407225E-7 9.874321 5 CTCTGCG 155 3.7014615E-8 9.781427 9 TCTGCGT 155 3.7014615E-8 9.781427 10 AGTGGTA 100 2.6798408E-4 9.475758 14 CGTTGAT 160 6.0485036E-8 9.475758 14 GGTATCA 225 1.8189894E-11 9.344472 1 CCATGTA 155 3.4766344E-7 9.248561 2 CATGTAC 155 3.539335E-7 9.2372675 3 CCCATGT 145 1.4205161E-6 9.2273 1 TTGATAC 165 9.721043E-8 9.188614 16 GCGTTGA 165 9.721043E-8 9.188614 13 TGATACC 170 1.5381192E-7 8.918361 17 TGGTATC 120 1.702696E-4 8.686111 16 GTTGATA 175 2.398192E-7 8.66355 15 >>END_MODULE