FastQCFastQC Report
Thu 26 May 2016
SRR1926830_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926830_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681978
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18380.26951016015179374No Hit
GTCCTAAAGTGTGTATTTCTCATTT14990.21980181178865008No Hit
CTGTAGGACGTGGAATATGGCAAGA14530.2130567261700524No Hit
GTCCTACAGTGTGCATTTCTCATTT13540.19854012886046177No Hit
GTATCAACGCAGAGTACTTTTTTTT12170.17845150430072523No Hit
CTTTAGGACGTGAAATATGGCGAGG11140.16334837780690872No Hit
CTGTAGGACCTGGAATATGGCGAGA9020.13226233104293686No Hit
GGTATCAACGCAGAGTACTTTTTTT7070.10366903331192501No Hit
TATCAACGCAGAGTACTTTTTTTTT6880.10088301968685207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4100.015.3307544
TGGCGAA456.858536E-414.74828418
TAGGACT1301.2732926E-1113.1865924
GGCGAGG4000.012.32535219
TGTAGGA8450.012.2994442
AGGACCT6550.012.2090385
TCCAACG701.10966954E-412.18990818
GTAGGAC8250.012.1353143
CTGTAGG8200.011.982941
GGACCTG6250.011.8811386
AGAACAG2650.011.8552725
GCAGCAC907.1454615E-611.6537533
GTCCTAG1655.456968E-1211.5634131
GTATAGG751.9916301E-411.4477791
GCTACAC1001.8410974E-611.4418683
TGTAGAA3600.011.3888952
TTAGGAC4950.011.3648183
GTAGAAC3950.011.3453123
GTCCTAC8500.011.2233121
CCAAGAC855.1176452E-511.2175173
Produced by FastQC (version 0.11.5)