##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926830_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 681978 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.663942238605937 31.0 30.0 33.0 23.0 34.0 2 28.810587731569054 31.0 30.0 33.0 23.0 34.0 3 28.60775714172598 31.0 29.0 33.0 16.0 34.0 4 32.22713049394555 35.0 33.0 37.0 22.0 37.0 5 32.038260765010016 35.0 32.0 37.0 19.0 37.0 6 31.78796676725642 35.0 32.0 37.0 17.0 37.0 7 31.578449744713172 35.0 32.0 37.0 17.0 37.0 8 31.397427776262578 35.0 32.0 37.0 17.0 37.0 9 33.61368255280962 37.0 34.0 39.0 20.0 39.0 10 33.80245550442976 38.0 34.0 39.0 23.0 39.0 11 33.95500734627803 38.0 35.0 39.0 25.0 39.0 12 33.85071659203083 38.0 34.0 39.0 23.0 39.0 13 33.958277246480094 38.0 35.0 39.0 25.0 39.0 14 34.99822721554068 39.0 35.0 41.0 25.0 41.0 15 34.61210332298109 39.0 34.0 41.0 18.0 41.0 16 34.69418221702166 39.0 34.0 41.0 20.0 41.0 17 34.922510696825995 39.0 35.0 41.0 24.0 41.0 18 34.97683209722308 39.0 36.0 41.0 24.0 41.0 19 34.37717638985422 39.0 34.0 41.0 18.0 41.0 20 34.674263685925354 39.0 34.0 41.0 18.0 41.0 21 34.65389499368015 39.0 34.0 41.0 18.0 41.0 22 34.67405106909607 39.0 35.0 41.0 18.0 41.0 23 34.67501004431228 39.0 35.0 41.0 18.0 41.0 24 33.40802635862154 39.0 33.0 41.0 2.0 41.0 25 33.986612471370044 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.20790073503110662 1101 2 -0.13061173955773242 1101 3 -0.860703204004512 1101 4 -0.4909694484150009 1101 5 -0.4649205950088948 1101 6 -2.63417546850334 1101 7 -0.8837800471125838 1101 8 -0.29930356091992394 1101 9 -0.48087298616567153 1101 10 -9.047659774543327 1101 11 -3.8399925674937876 1101 12 -1.5100416663666891 1101 13 -0.8339011418301325 1101 14 -0.526992932080887 1101 15 -2.02608300791519 1101 16 -0.5965705078988037 1101 17 -0.4680376219229885 1101 18 -0.3309524963452475 1101 19 -1.0427962114064684 1101 20 -0.21525482212995684 1101 21 -0.23454351788593186 1101 22 -0.01877210618295777 1101 23 -0.16797609764707033 1101 24 -0.19380484199287196 1101 25 -0.21718669757483866 1102 1 -0.3861916144195412 1102 2 -0.3756895039670205 1102 3 -0.5845834131805248 1102 4 -0.5170909552432263 1102 5 -0.6996268297916153 1102 6 -1.3707364587260358 1102 7 -0.5357390865816605 1102 8 -0.04733530405664155 1102 9 -0.09776180183838079 1102 10 0.03463292479018065 1102 11 -0.3677540840885243 1102 12 -0.1198693697352553 1102 13 -0.4338701556964253 1102 14 -0.6186177527584462 1102 15 -0.004372122270694945 1102 16 -0.02969586151824899 1102 17 -0.34820855555676644 1102 18 -0.46897611052473565 1102 19 -0.07078276970010933 1102 20 -0.19543056057560904 1102 21 -0.3468358936300646 1102 22 -0.4562963140999159 1102 23 -0.6335564846011223 1102 24 -3.795201571386851 1102 25 -1.9564462270366718 1103 1 0.2533546257607142 1103 2 0.1540387142850932 1103 3 0.4807623370509724 1103 4 0.31519176423578443 1103 5 0.28495842595231835 1103 6 0.9085110302359318 1103 7 0.620153975719635 1103 8 0.632367282054183 1103 9 0.5737243152670715 1103 10 0.7299366707300692 1103 11 0.41029690057068535 1103 12 0.2737065875188449 1103 13 0.4176852998971583 1103 14 0.43730894539624643 1103 15 0.819947410231471 1103 16 0.6406444076901536 1103 17 0.3815800451309457 1103 18 0.18541346965523786 1103 19 -1.1647068377742187 1103 20 -0.04805689258579804 1103 21 -0.02424374456244749 1103 22 0.20038901438661583 1103 23 0.3875155670440975 1103 24 1.6196591944164282 1103 25 0.7658350943255385 1104 1 -0.4314078329641049 1104 2 -0.5111896272488714 1104 3 -1.4313009967374022 1104 4 -0.613515187405234 1104 5 -0.8153929448091262 1104 6 -1.926559372514891 1104 7 -0.6930794068768265 1104 8 -0.2961210520804194 1104 9 -0.15776887197832679 1104 10 -0.07434823239567123 1104 11 -0.2131972600666856 1104 12 -0.22001879328119855 1104 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7 1.2285068969855537 1110 8 1.3349792153361193 1110 9 0.8734572925701443 1110 10 1.003939566610022 1110 11 0.8298779381983223 1110 12 1.0348190567266116 1110 13 0.96271999404334 1110 14 0.7678473158056178 1110 15 1.126252530673753 1110 16 0.9735784606057791 1110 17 0.6541838925143111 1110 18 0.7858553734142362 1110 19 -1.479904566200851 1110 20 0.5781075708302126 1110 21 0.6938724291151317 1110 22 0.7963968370233658 1110 23 0.8662684582229403 1110 24 -0.4151891479743668 1110 25 0.2192245901791665 1111 1 0.15748949963925796 1111 2 -0.11392920402811058 1111 3 0.5389907472164914 1111 4 0.36380662979107115 1111 5 0.4859659484650436 1111 6 0.37670616596257034 1111 7 0.40719106436415586 1111 8 0.6721414766213982 1111 9 0.30311357789732796 1111 10 0.11854161526338203 1111 11 0.035238609921897535 1111 12 -0.06049251290085067 1111 13 0.03626009121198592 1111 14 0.22829121946540454 1111 15 -0.5578112934216577 1111 16 -0.12051720650099185 1111 17 -0.20334408877684496 1111 18 -0.03620432575991828 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-6.126031484488728 2216 10 -3.6223324998489623 2216 11 -2.513280461034846 2216 12 -2.33699179078517 2216 13 -1.7860378939668848 2216 14 -1.9608131203314514 2216 15 -3.2068251911890187 2216 16 -2.1430921999901145 2216 17 -2.3195360531682567 2216 18 -2.025391531290609 2216 19 -1.482588486641781 2216 20 -2.3948934675913875 2216 21 -5.098786728964587 2216 22 -3.5033874907983424 2216 23 -3.421270482587026 2216 24 -3.5971046364319115 2216 25 -3.1344538186394324 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33359.0 3 5.0 4 236.0 5 1592.0 6 2220.0 7 1757.0 8 1378.0 9 1194.0 10 1136.0 11 1139.0 12 1102.0 13 1024.0 14 1131.0 15 1159.0 16 1223.0 17 1344.0 18 1540.0 19 1891.0 20 2506.0 21 3303.0 22 4017.0 23 4923.0 24 5641.0 25 6582.0 26 7528.0 27 8197.0 28 9173.0 29 10993.0 30 13457.0 31 16286.0 32 21131.0 33 28085.0 34 38584.0 35 54100.0 36 79487.0 37 127579.0 38 185976.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.952452905623105 18.973712988876084 12.522047929258484 25.551786176242324 2 13.372805046740499 20.377565734793553 43.90934428203479 22.340284936431157 3 18.64783106699169 25.30911333666187 30.04229199095421 26.00076360539223 4 10.84285374707638 17.612740969424138 40.227844312255876 31.316560971243607 5 11.612899440914717 39.66733545703608 36.61891268765729 12.1008524143919 6 30.453421487215437 38.80628536397209 19.06889906400054 11.671394084811935 7 26.754547874569994 32.465257606264146 23.00594323264204 17.77425128652382 8 23.19960587655701 39.19454697863204 21.25869498080854 16.347152164002413 9 26.099837061176125 15.707390617598275 20.610807933111484 37.581964388114116 10 13.36198676856728 30.538058705314576 36.200730515908525 19.899224010209622 11 33.72841635356264 22.8724324736538 25.49825336348518 17.90089780929838 12 22.674670135553498 25.465080638419877 33.435356541046616 18.424892684980005 13 28.75788621558442 23.004578477927396 26.081485761549583 22.156049544938597 14 19.256541231647812 23.6776380144515 28.799164646477983 28.266656107422712 15 21.927501290534515 33.30253648693041 23.558707588342955 21.211254634192127 16 20.225562807069004 29.37229595952189 30.104421793649628 20.29771943975948 17 20.185639008536928 30.01814152737875 29.09625275532697 20.699966708757355 18 19.675020897798763 29.100001466512193 31.668890876827643 19.556086758861397 19 22.787245900677597 27.647179607521043 28.2329647120941 21.332609779707255 20 22.389359895464406 28.69463961764141 29.55007750710176 19.365922979792423 21 21.844267223553498 27.8756273042708 27.85230962360277 22.427795848572927 22 21.647662694775434 30.060054995417048 28.383061411549036 19.90922089825848 23 20.907473701037294 29.31727555505367 28.791369628566105 20.983881115342932 24 22.121699189591098 28.11250692939206 29.65398502371392 20.11180885730292 25 21.47224238691275 28.688561854271615 29.408624621453765 20.430571137361873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 253.0 2 324.0 3 395.0 4 395.0 5 395.0 6 639.5 7 884.0 8 884.0 9 884.0 10 1354.5 11 1825.0 12 1825.0 13 1825.0 14 2973.5 15 4122.0 16 4122.0 17 4122.0 18 7080.0 19 10038.0 20 10038.0 21 10038.0 22 15429.0 23 20820.0 24 20820.0 25 20820.0 26 29935.5 27 39051.0 28 39051.0 29 39051.0 30 50929.0 31 62807.0 32 62807.0 33 62807.0 34 69837.5 35 76868.0 36 76868.0 37 76868.0 38 81299.5 39 85731.0 40 85731.0 41 85731.0 42 87862.0 43 89993.0 44 89993.0 45 89993.0 46 90122.5 47 90252.0 48 90252.0 49 90252.0 50 83642.5 51 77033.0 52 77033.0 53 77033.0 54 65074.0 55 53115.0 56 53115.0 57 53115.0 58 43701.0 59 34287.0 60 34287.0 61 34287.0 62 26741.0 63 19195.0 64 19195.0 65 19195.0 66 14319.0 67 9443.0 68 9443.0 69 9443.0 70 6660.0 71 3877.0 72 3877.0 73 3877.0 74 2612.0 75 1347.0 76 1347.0 77 1347.0 78 903.5 79 460.0 80 460.0 81 460.0 82 292.0 83 124.0 84 124.0 85 124.0 86 82.5 87 41.0 88 41.0 89 41.0 90 24.0 91 7.0 92 7.0 93 7.0 94 5.0 95 3.0 96 3.0 97 3.0 98 5.0 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24077023012472545 2 0.1442861793195675 3 0.1463390314643581 4 0.06745085618597667 5 0.021994844408470653 6 0.020528521447905942 7 0.29986304543548326 8 0.2915050045602644 9 0.01862230159917182 10 0.03929745534313424 11 0.014076700421421218 12 0.0156896556780424 13 0.013490171237195335 14 0.016276184862268284 15 0.013196906645082393 16 0.01876893389522829 17 0.01715597863860711 18 0.01290364205296945 19 0.023167902776922423 20 0.015396391085929459 21 0.013490171237195335 22 0.015103126493816517 23 0.0156896556780424 24 0.014663229605647103 25 0.013636803533251805 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 681978.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.95139802009818 #Duplication Level Percentage of deduplicated Percentage of total 1 95.04648255311768 81.69378052333336 2 3.7310912582491724 6.413850195741672 3 0.5391669381667111 1.3902645630493398 4 0.1968898490025169 0.6769183111092945 5 0.11381284403762938 0.48911865288388207 6 0.07296925142458444 0.3763085503453844 7 0.04687403025778582 0.2820221902045152 8 0.03193799151026398 0.21960920162089728 9 0.024749910561450577 0.1914560472266163 >10 0.16135949435050004 2.684205057973169 >50 0.017760133583862647 1.0408079462971427 >100 0.01468579933122508 2.6340241134074636 >500 0.0011953557574194854 0.6637165401909947 >1k 0.0010245906492167018 1.2439181066162495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1838 0.26951016015179374 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1499 0.21980181178865008 No Hit CTGTAGGACGTGGAATATGGCAAGA 1453 0.2130567261700524 No Hit GTCCTACAGTGTGCATTTCTCATTT 1354 0.19854012886046177 No Hit GTATCAACGCAGAGTACTTTTTTTT 1217 0.17845150430072523 No Hit CTTTAGGACGTGAAATATGGCGAGG 1114 0.16334837780690872 No Hit CTGTAGGACCTGGAATATGGCGAGA 902 0.13226233104293686 No Hit GGTATCAACGCAGAGTACTTTTTTT 707 0.10366903331192501 No Hit TATCAACGCAGAGTACTTTTTTTTT 688 0.10088301968685207 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 410 0.0 15.330754 4 TGGCGAA 45 6.858536E-4 14.748284 18 TAGGACT 130 1.2732926E-11 13.186592 4 GGCGAGG 400 0.0 12.325352 19 TGTAGGA 845 0.0 12.299444 2 AGGACCT 655 0.0 12.209038 5 TCCAACG 70 1.10966954E-4 12.189908 18 GTAGGAC 825 0.0 12.135314 3 CTGTAGG 820 0.0 11.98294 1 GGACCTG 625 0.0 11.881138 6 AGAACAG 265 0.0 11.855272 5 GCAGCAC 90 7.1454615E-6 11.653753 3 GTCCTAG 165 5.456968E-12 11.563413 1 GTATAGG 75 1.9916301E-4 11.447779 1 GCTACAC 100 1.8410974E-6 11.441868 3 TGTAGAA 360 0.0 11.388895 2 TTAGGAC 495 0.0 11.364818 3 GTAGAAC 395 0.0 11.345312 3 GTCCTAC 850 0.0 11.223312 1 CCAAGAC 85 5.1176452E-5 11.217517 3 >>END_MODULE