FastQCFastQC Report
Thu 26 May 2016
SRR1926830_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926830_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681978
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22240.32611022642959153No Hit
GTCCTACAGTGGACATTTCTAAATT18250.2676039403030596No Hit
CTGTAGGACGTGGAATATGGCAAGA16370.24003706864444305No Hit
GTCCTAAAGTGTGTATTTCTCATTT14780.2167225335714642No Hit
GTCCTACAGTGTGCATTTCTCATTT14320.2099774479528665No Hit
CTTTAGGACGTGAAATATGGCGAGG14040.20587174366328534No Hit
GGTATCAACGCAGAGTACTTTTTTT12760.18710280976805702No Hit
TATCAACGCAGAGTACTTTTTTTTT11180.1639349069911346No Hit
CTGTAGGACCTGGAATATGGCGAGA10910.1599758349976099No Hit
CTGAAGGACCTGGAATATGGCGAGA7490.1098275897462968No Hit
CACACACACACACACACACACACAC7250.1063084146409415No Hit
ACGCAGAGTACTTTTTTTTTTTTTT6860.10058975509473912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA603.765308E-918.9766819
TCCAACG855.820766E-1116.74413118
ACCGTGC350.002187452516.2645348
TATGTCG456.8186363E-414.75964116
ATGTCGA500.00151196913.28367717
GACGTGA4300.012.7982267
TTAGGAC5150.012.5743983
TAGGACC3300.012.4072664
AGGACGA550.003013082812.1187265
GGACGAG550.003013082812.1187266
TGTCGAG550.003093731112.07607118
GGACGTG8550.012.0276086
AGGACGT8500.011.9863365
CTAGAGT1209.635187E-911.902324
ACGTGAA4950.011.8835148
CTAATAC657.850522E-411.7209333
CTCTTAG907.131286E-611.6558361
CTTTAGG5650.011.6464591
CCTACAC1406.493792E-1011.5639553
GCATTAA751.9978976E-411.4439121
Produced by FastQC (version 0.11.5)