##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926830_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 681978 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.13525802885137 31.0 30.0 33.0 26.0 34.0 2 30.446061309895626 31.0 30.0 34.0 27.0 34.0 3 30.488744504954706 31.0 31.0 34.0 26.0 34.0 4 34.06484666660801 37.0 35.0 37.0 30.0 37.0 5 34.057278973808536 37.0 35.0 37.0 30.0 37.0 6 34.04591497086416 37.0 35.0 37.0 30.0 37.0 7 34.04169929235254 37.0 35.0 37.0 30.0 37.0 8 34.08284431462599 37.0 35.0 37.0 30.0 37.0 9 35.639170178510156 39.0 37.0 39.0 30.0 39.0 10 35.541741815718396 39.0 35.0 39.0 30.0 39.0 11 35.577523028602094 39.0 37.0 39.0 30.0 39.0 12 35.475420028212056 39.0 35.0 39.0 30.0 39.0 13 35.503720061350954 39.0 35.0 39.0 30.0 39.0 14 36.62335295273454 40.0 37.0 41.0 30.0 41.0 15 36.638407690570666 40.0 37.0 41.0 30.0 41.0 16 36.62083820885718 40.0 37.0 41.0 30.0 41.0 17 36.61016337770427 40.0 37.0 41.0 30.0 41.0 18 36.5742692579526 40.0 37.0 41.0 30.0 41.0 19 36.642247990404385 40.0 37.0 41.0 30.0 41.0 20 36.60552833082592 40.0 37.0 41.0 30.0 41.0 21 36.56548598341882 40.0 37.0 41.0 30.0 41.0 22 36.488251820439956 40.0 37.0 41.0 30.0 41.0 23 36.420821492775424 40.0 37.0 41.0 30.0 41.0 24 36.370096982600614 40.0 37.0 41.0 30.0 41.0 25 36.2652153002003 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.2758436658421779 1101 2 -0.1325708013071072 1101 3 0.008953313254039585 1101 4 -0.2955713688662982 1101 5 -0.14903857228527784 1101 6 -0.1404030011907338 1101 7 -0.09302927898907853 1101 8 -0.15479652548206957 1101 9 -0.27841550141000226 1101 10 0.05043965512765425 1101 11 -0.020493101763669586 1101 12 0.12066712436712379 1101 13 0.024692023192947943 1101 14 -0.022080402493564577 1101 15 -0.1401908166764798 1101 16 -0.22813450101470778 1101 17 -0.1459873234348663 1101 18 -0.15139600105347029 1101 19 -0.277456881337784 1101 20 -0.2859715626766004 1101 21 -0.1591739045466838 1101 22 -0.23251718885414618 1101 23 -0.3273383326589112 1101 24 -0.202008263214978 1101 25 -0.17494098266919877 1102 1 -0.667418183081157 1102 2 -0.5639301414201263 1102 3 -0.424127914542094 1102 4 -0.5042090478087644 1102 5 -0.6631799180836708 1102 6 -0.5037870007749703 1102 7 -0.6259113522359883 1102 8 -0.5049957290599849 1102 9 -0.46870370187068033 1102 10 -0.4361691380702979 1102 11 -0.4705745384220279 1102 12 -0.409985123941631 1102 13 -0.522426021229009 1102 14 -0.4672334749746483 1102 15 -0.3952910264212264 1102 16 -0.45437642231188846 1102 17 -0.37567105895056585 1102 18 -0.5459289096022104 1102 19 -0.28046724306167903 1102 20 -0.5055838241642618 1102 21 -0.4899891242295453 1102 22 -0.6265686356418314 1102 23 -0.576873785796792 1102 24 -0.7376996805462284 1102 25 -0.6697964019576688 1103 1 -0.3379073821223315 1103 2 -0.3253810219297719 1103 3 -0.3195660012699122 1103 4 -0.29450165947068285 1103 5 -0.4573264690916261 1103 6 -0.3363057791045634 1103 7 -0.3365791860262419 1103 8 -0.23695647642433215 1103 9 -0.35421009369594714 1103 10 -0.15155713574415586 1103 11 -0.2664606304019088 1103 12 -0.5448537516190797 1103 13 -0.3996632613869764 1103 14 -0.36777874052565807 1103 15 -0.3462323237520408 1103 16 -0.4414651176257607 1103 17 -0.38898964113855783 1103 18 -0.3045339132393394 1103 19 -0.22048701932246217 1103 20 -0.14753725674362528 1103 21 -0.3160411058068249 1103 22 -0.25017229181857203 1103 23 -0.10086263328251732 1103 24 -0.20212875246008366 1103 25 -0.1191035236404403 1104 1 0.13573783233056247 1104 2 0.1900794894175668 1104 3 0.31670689668814234 1104 4 0.2212378117717222 1104 5 0.11837355207767075 1104 6 0.19083642515342802 1104 7 0.1757682515985266 1104 8 0.11677851958911134 1104 9 0.28091234199983717 1104 10 0.05223197057517126 1104 11 0.07941699523391321 1104 12 0.16973367460987987 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0.5876418639299601 2214 6 0.72406775663638 2214 7 0.7547970470455212 2214 8 0.731408011109032 2214 9 0.6932860036526947 2214 10 0.6002202531766798 2214 11 0.702750815510754 2214 12 0.6843782870071209 2214 13 0.6080144294321386 2214 14 0.4394259122388888 2214 15 0.6114573416076254 2214 16 0.6053509656680944 2214 17 0.530749471207109 2214 18 0.3876653291306056 2214 19 0.3963477862092475 2214 20 0.5473523240076048 2214 21 0.4543064355665223 2214 22 0.6226047702060526 2214 23 0.6277339680456961 2214 24 0.507726381820838 2214 25 0.5981600928106587 2215 1 0.40589243807545117 2215 2 0.3079371851852599 2215 3 0.34275430353765657 2215 4 0.21472219429018224 2215 5 0.4104675289391082 2215 6 0.14531329705451412 2215 7 0.1447299453578097 2215 8 0.315808624821436 2215 9 0.08347473870269795 2215 10 0.15241649326393514 2215 11 -0.11241379620523873 2215 12 -0.06089039702342092 2215 13 0.21354449579440882 2215 14 -0.010078496508363344 2215 15 0.028633894836083584 2215 16 -0.13287998983776106 2215 17 0.0341688844832575 2215 18 0.07474002828212889 2215 19 0.006258132439171504 2215 20 -0.18516814847700402 2215 21 0.1820007624238329 2215 22 0.11735457148893147 2215 23 0.24374372829118585 2215 24 0.1661858158005458 2215 25 0.10190336877790429 2216 1 -0.125135021462647 2216 2 -0.08884041074127325 2216 3 -1.734030566438701 2216 4 -1.2302787149660475 2216 5 -0.36280033794577804 2216 6 -0.11634154928118079 2216 7 -0.3493962594066957 2216 8 -0.11158544682795224 2216 9 -0.2800058941396415 2216 10 -0.19166788571406101 2216 11 -0.029213214281249122 2216 12 -0.06854633446658909 2216 13 -0.05949060149600882 2216 14 -0.11093683438797797 2216 15 -0.009573051881496042 2216 16 -0.0666934104596919 2216 17 -0.2271622666351263 2216 18 -0.2693879949948297 2216 19 -0.3772188633782534 2216 20 -0.30815050890617357 2216 21 -0.14634257272800966 2216 22 -0.10655159326829988 2216 23 -0.22948049429474082 2216 24 -0.18019716292285892 2216 25 -0.3716736452246465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14551.0 3 11.0 4 235.0 5 1456.0 6 2029.0 7 1511.0 8 1225.0 9 991.0 10 898.0 11 831.0 12 770.0 13 758.0 14 713.0 15 764.0 16 801.0 17 809.0 18 937.0 19 1159.0 20 1383.0 21 1888.0 22 2283.0 23 2847.0 24 3483.0 25 4035.0 26 4830.0 27 5456.0 28 6410.0 29 7529.0 30 9961.0 31 12881.0 32 16761.0 33 22538.0 34 31270.0 35 43910.0 36 65333.0 37 115768.0 38 292963.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.691707635012044 19.762571985138365 10.782173668596057 27.763546711253532 2 13.336729630293013 21.35071806722319 41.96017230089821 23.35238000158559 3 18.201256847029835 25.671357233469966 29.618032895074535 26.509353024425657 4 11.250216282636684 18.128150761461516 39.315931012437346 31.305701943464452 5 11.89121495984937 39.66524521506543 36.13996703483632 12.303572790248882 6 30.240822380618848 39.35310805958904 18.56065733944684 11.845412220345269 7 27.295500812540702 32.37916023771949 22.327709104562548 17.99762984517726 8 23.796102124913844 39.86919094895221 20.214691088266783 16.120015837867165 9 26.135197123365618 15.660470327375538 20.255664987065707 37.94866756219314 10 13.548654827210513 30.913972581849002 35.394167080915665 20.143205510024824 11 33.71068426138688 23.022170344616224 25.268825876497736 17.998319517499162 12 22.917995527695297 25.69918252135342 32.892261446533965 18.490560504417317 13 28.928375568570065 23.158947408552233 25.91242481384921 22.0002522090285 14 19.477607782069217 23.914407796145916 28.510597116036003 28.097387305748867 15 21.698046806264298 33.3218957123585 23.60754296439674 21.372514516980466 16 20.194405668234662 29.555878799253932 29.86732670944432 20.38238882306709 17 20.134080571514037 30.156398006973834 28.95240022405415 20.757121197457984 18 19.759961875435316 28.928333150042157 31.59763920964845 19.71406576487408 19 22.390344542411256 27.83539620837653 28.37353752399274 21.40072172521947 20 22.296630511765127 28.76096267740805 29.493836266245143 19.448570544581678 21 21.60960029095346 27.686040099956593 28.15238687424475 22.551972734845197 22 21.680904662613752 29.9392355765142 28.30604506303722 20.073814697834827 23 20.72330991119922 29.44751721468204 28.92125236078689 20.90792051333185 24 21.838974277762627 27.919229066040252 30.06665904178727 20.17513761440985 25 21.288089861278117 28.680089157403877 29.40024049036572 20.631580490952285 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 253.0 1 253.0 2 261.5 3 270.0 4 270.0 5 270.0 6 532.0 7 794.0 8 794.0 9 794.0 10 1268.0 11 1742.0 12 1742.0 13 1742.0 14 2775.0 15 3808.0 16 3808.0 17 3808.0 18 6654.5 19 9501.0 20 9501.0 21 9501.0 22 14986.0 23 20471.0 24 20471.0 25 20471.0 26 29110.0 27 37749.0 28 37749.0 29 37749.0 30 49886.5 31 62024.0 32 62024.0 33 62024.0 34 69234.5 35 76445.0 36 76445.0 37 76445.0 38 80514.0 39 84583.0 40 84583.0 41 84583.0 42 87077.5 43 89572.0 44 89572.0 45 89572.0 46 90285.5 47 90999.0 48 90999.0 49 90999.0 50 85059.5 51 79120.0 52 79120.0 53 79120.0 54 66765.5 55 54411.0 56 54411.0 57 54411.0 58 44659.5 59 34908.0 60 34908.0 61 34908.0 62 27336.0 63 19764.0 64 19764.0 65 19764.0 66 14721.0 67 9678.0 68 9678.0 69 9678.0 70 6751.0 71 3824.0 72 3824.0 73 3824.0 74 2630.5 75 1437.0 76 1437.0 77 1437.0 78 939.0 79 441.0 80 441.0 81 441.0 82 293.5 83 146.0 84 146.0 85 146.0 86 86.0 87 26.0 88 26.0 89 26.0 90 18.0 91 10.0 92 10.0 93 10.0 94 5.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11158717729897445 2 0.12375765787166154 3 0.016569449454381227 4 0.0 5 0.0067450856185976675 6 0.3504511875749658 7 0.02434096114537419 8 0.00997099613184003 9 0.010264260723952971 10 0.0030792782171858916 11 0.004545601177750602 12 4.398968881694131E-4 13 5.86529184225884E-4 14 0.0 15 0.0026393813290164787 16 2.93264592112942E-4 17 0.0 18 4.398968881694131E-4 19 1.46632296056471E-4 20 7.331614802823552E-4 21 0.012023848276630625 22 0.0 23 2.93264592112942E-4 24 0.0 25 0.005572027250145899 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 681978.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.64419550798387 #Duplication Level Percentage of deduplicated Percentage of total 1 93.88663915014351 78.53072400662137 2 4.694170257773582 7.852801895779531 3 0.619232726510872 1.5538566972365189 4 0.24808493084143568 0.8300345783154282 5 0.13174555687444117 0.5509875558256982 6 0.08412674645574826 0.4222028416797118 7 0.059795640518626236 0.35010907722455664 8 0.03671049228603503 0.2456495675173958 9 0.029418884849129325 0.22146470603526938 >10 0.17078941088711286 2.7744579737199677 >50 0.01981873614925261 1.1542378802942783 >100 0.0168367279690351 2.9591789545345755 >500 0.0010522954965040733 0.5760804950323039 >1k 0.0015784432447561098 1.9782137701835032 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2224 0.32611022642959153 No Hit GTCCTACAGTGGACATTTCTAAATT 1825 0.2676039403030596 No Hit CTGTAGGACGTGGAATATGGCAAGA 1637 0.24003706864444305 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1478 0.2167225335714642 No Hit GTCCTACAGTGTGCATTTCTCATTT 1432 0.2099774479528665 No Hit CTTTAGGACGTGAAATATGGCGAGG 1404 0.20587174366328534 No Hit GGTATCAACGCAGAGTACTTTTTTT 1276 0.18710280976805702 No Hit TATCAACGCAGAGTACTTTTTTTTT 1118 0.1639349069911346 No Hit CTGTAGGACCTGGAATATGGCGAGA 1091 0.1599758349976099 No Hit CTGAAGGACCTGGAATATGGCGAGA 749 0.1098275897462968 No Hit CACACACACACACACACACACACAC 725 0.1063084146409415 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 686 0.10058975509473912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.4663229605647103E-4 0.0 5 0.0 0.0 0.0 1.4663229605647103E-4 0.0 6 0.0 0.0 0.0 1.4663229605647103E-4 0.0 7 0.0 0.0 0.0 1.4663229605647103E-4 0.0 8 0.0 0.0 0.0 1.4663229605647103E-4 0.0 9 0.0 0.0 0.0 1.4663229605647103E-4 0.0 10 0.0 0.0 0.0 1.4663229605647103E-4 0.0 11 0.0 0.0 0.0 1.4663229605647103E-4 0.0 12 0.0 0.0 0.0 1.4663229605647103E-4 0.0 13 0.0 0.0 0.0 1.4663229605647103E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACGA 60 3.765308E-9 18.97668 19 TCCAACG 85 5.820766E-11 16.744131 18 ACCGTGC 35 0.0021874525 16.264534 8 TATGTCG 45 6.8186363E-4 14.759641 16 ATGTCGA 50 0.001511969 13.283677 17 GACGTGA 430 0.0 12.798226 7 TTAGGAC 515 0.0 12.574398 3 TAGGACC 330 0.0 12.407266 4 AGGACGA 55 0.0030130828 12.118726 5 GGACGAG 55 0.0030130828 12.118726 6 TGTCGAG 55 0.0030937311 12.076071 18 GGACGTG 855 0.0 12.027608 6 AGGACGT 850 0.0 11.986336 5 CTAGAGT 120 9.635187E-9 11.90232 4 ACGTGAA 495 0.0 11.883514 8 CTAATAC 65 7.850522E-4 11.720933 3 CTCTTAG 90 7.131286E-6 11.655836 1 CTTTAGG 565 0.0 11.646459 1 CCTACAC 140 6.493792E-10 11.563955 3 GCATTAA 75 1.9978976E-4 11.443912 1 >>END_MODULE