FastQCFastQC Report
Thu 26 May 2016
SRR1926829_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926829_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700333
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11550.1649215444652758No Hit
GTCCTAAAGTGTGTATTTCTCATTT10660.1522133042424104No Hit
GTCCTACAGTGTGCATTTCTCATTT9620.1373632257797362No Hit
CTGTAGGACGTGGAATATGGCAAGA9440.13479301989196568No Hit
GTATCAACGCAGAGTACTTTTTTTT9350.1335079169480804No Hit
CTTTAGGACGTGAAATATGGCGAGG8280.11822947083744448No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAG250.00609574518.96372811
CAATACT551.920882E-413.8491564
GTAGCAC853.7761474E-612.339373
TAACAGG701.0695679E-412.2381556
GTCCTAT959.817304E-712.0545571
CCTACAC802.755458E-511.918713
TAACACT802.7962422E-511.9016184
TACTGTG1209.686119E-911.8982077
TAGGACC3950.011.8112274
GTAGGAC6750.011.3007033
TAGGACA2400.011.1081784
GGACCTT600.005785468511.1049936
ACTTAGT600.00582262311.0954498
CAGTGCG600.005954277711.0621759
GTGTAGA1303.041896E-811.0113741
GTCCTAC5650.010.9788921
GGTATCA3400.010.6659181
TGTAGGA7450.010.622852
GGACGTG4850.010.597996
AGGACGT5050.010.555245
Produced by FastQC (version 0.11.5)