##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926829_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 700333 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.634151182366104 31.0 30.0 33.0 23.0 34.0 2 28.782513461453338 31.0 30.0 33.0 23.0 34.0 3 28.57103692100758 31.0 29.0 33.0 16.0 34.0 4 32.21964265570807 35.0 33.0 37.0 22.0 37.0 5 32.00919419761742 35.0 32.0 37.0 19.0 37.0 6 31.747888504468587 35.0 32.0 37.0 17.0 37.0 7 31.542750377320502 35.0 32.0 37.0 17.0 37.0 8 31.33814057027157 35.0 32.0 37.0 17.0 37.0 9 33.55690078862484 37.0 34.0 39.0 19.0 39.0 10 33.75044014775828 38.0 34.0 39.0 22.0 39.0 11 33.90426125857271 38.0 34.0 39.0 24.0 39.0 12 33.80339352850715 38.0 34.0 39.0 23.0 39.0 13 33.90440690357301 38.0 35.0 39.0 24.0 39.0 14 34.96857209356121 39.0 35.0 41.0 24.0 41.0 15 34.57044720154555 39.0 34.0 41.0 18.0 41.0 16 34.64716499151118 39.0 34.0 41.0 19.0 41.0 17 34.87327885448779 39.0 35.0 41.0 24.0 41.0 18 34.92776150773989 39.0 35.0 41.0 24.0 41.0 19 34.32800681961296 39.0 34.0 41.0 18.0 41.0 20 34.60952860996126 39.0 34.0 41.0 18.0 41.0 21 34.60222636945567 39.0 34.0 41.0 18.0 41.0 22 34.616299674583374 39.0 35.0 41.0 18.0 41.0 23 34.617075020026185 39.0 35.0 41.0 18.0 41.0 24 33.35005633034571 39.0 33.0 41.0 2.0 41.0 25 33.93009611142128 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6967934140782219 1101 2 -0.8809597968089697 1101 3 -1.4806115221514489 1101 4 -1.0595038486164583 1101 5 -0.8207894259585196 1101 6 -2.6440575766426555 1101 7 -1.3788635687331237 1101 8 -0.8122105771904273 1101 9 -0.8213209386158553 1101 10 -9.335572758976895 1101 11 -4.598257070969751 1101 12 -2.0684252992702348 1101 13 -1.4697419347879048 1101 14 -1.340172242773285 1101 15 -2.5038692364262403 1101 16 -0.9968413839577366 1101 17 -1.0316260686581273 1101 18 -1.0278064664765196 1101 19 -1.6041680221933845 1101 20 -0.8578051422845689 1101 21 -1.0222988154755868 1101 22 -0.8237585279850634 1101 23 -0.7810286099920418 1101 24 -0.7303337932582821 1101 25 -0.5996160478085812 1102 1 0.23077972923973888 1102 2 0.27970462224804393 1102 3 0.2189079489335093 1102 4 0.296799725311395 1102 5 0.37929702544921895 1102 6 -0.1741713249871779 1102 7 0.3368217443342729 1102 8 1.1106239245381282 1102 9 0.9291058438017572 1102 10 0.8889390714412428 1102 11 0.6249151434716325 1102 12 0.8128449270744014 1102 13 0.5251709520892831 1102 14 0.5324652202016154 1102 15 0.8484945145365757 1102 16 0.7957361723808773 1102 17 0.7942086206357004 1102 18 0.4504973721373631 1102 19 0.6599734001226949 1102 20 0.6949725852430646 1102 21 0.5588260239752003 1102 22 0.4068963033442401 1102 23 0.1663350505205159 1102 24 -3.1203410388002766 1102 25 -1.2572084903560068 1103 1 -0.18837465163261413 1103 2 -0.3514895187648648 1103 3 -0.03426407764964168 1103 4 -0.18411941761122108 1103 5 -0.7156221938617584 1103 6 0.27735071305422565 1103 7 0.06678507792223343 1103 8 0.15087390544103485 1103 9 -0.22599403397686757 1103 10 -0.09686415214748223 1103 11 -0.1320123257820498 1103 12 -0.24515293405156058 1103 13 -0.18577740938042808 1103 14 -0.31719580645792433 1103 15 -0.19402706517467294 1103 16 -0.06806529427560548 1103 17 -0.49551905486654846 1103 18 -0.5251901230883647 1103 19 -1.9511239186459264 1103 20 -0.5805898240457594 1103 21 -0.34560068139489886 1103 22 -0.3425670684378943 1103 23 -0.32469886591061936 1103 24 0.9166423849259502 1103 25 0.24363751071343387 1104 1 -0.1773206293190519 1104 2 -0.21437119815417915 1104 3 -1.5076492802333163 1104 4 -0.6679641034597275 1104 5 0.029760953525208578 1104 6 -1.5223410074390245 1104 7 -0.2603435186720091 1104 8 0.22244613092547283 1104 9 0.10657349391508575 1104 10 0.30937483787013065 1104 11 -0.1359392194990221 1104 12 0.09887231550803932 1104 13 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-0.8513097308680173 2215 1 0.5372022714443077 2215 2 0.03684381456846708 2215 3 0.5306077172221535 2215 4 0.7180600695682671 2215 5 0.9229034471638826 2215 6 1.3127353284388406 2215 7 0.9637722574094134 2215 8 -0.2656645560974269 2215 9 0.1445187865359543 2215 10 -0.2520564598397925 2215 11 -0.004768736038464283 2215 12 -0.3753452417438652 2215 13 -0.06661074271376322 2215 14 0.06299650123438028 2215 15 0.20167806303044955 2215 16 0.3357808595705478 2215 17 0.04544248359498937 2215 18 -0.6473055077037486 2215 19 0.5880427480207402 2215 20 -3.3124487984047377 2215 21 -1.8051519634461854 2215 22 -0.9009645043353345 2215 23 -0.8195065582183148 2215 24 -0.7329088971253341 2215 25 -0.33501633544041454 2216 1 -0.4752133403700398 2216 2 -1.1673650461910263 2216 3 -0.3308479789803762 2216 4 -0.4906635591237176 2216 5 -0.46694887351122105 2216 6 -1.8883828150210782 2216 7 -10.014698206725608 2216 8 -11.29246835356578 2216 9 -6.338867289413415 2216 10 -3.8364129999241783 2216 11 -2.5774480609329764 2216 12 -2.184279840899986 2216 13 -1.8407352152876015 2216 14 -1.8825520219723657 2216 15 -3.2508430340159613 2216 16 -2.0501896045644727 2216 17 -2.348724183071681 2216 18 -2.0040038199400385 2216 19 -1.4982758173801045 2216 20 -2.3321112456621194 2216 21 -4.813516942349139 2216 22 -3.1237176688922936 2216 23 -3.1348335624377235 2216 24 -3.489670500500864 2216 25 -2.8625374324868282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34967.0 3 7.0 4 284.0 5 1718.0 6 2294.0 7 1817.0 8 1435.0 9 1244.0 10 1176.0 11 1072.0 12 1099.0 13 1085.0 14 1135.0 15 1202.0 16 1268.0 17 1361.0 18 1550.0 19 1999.0 20 2602.0 21 3303.0 22 4294.0 23 4907.0 24 5839.0 25 6822.0 26 7802.0 27 8561.0 28 9510.0 29 11279.0 30 13758.0 31 17046.0 32 21725.0 33 29145.0 34 39322.0 35 54792.0 36 81056.0 37 129967.0 38 191890.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62203570324398 19.070690819983742 12.503864607069653 25.803408869702626 2 13.985601018080946 20.670188959827268 42.444252203816376 22.89995781827541 3 18.132095194107496 25.384904672907886 30.39585087991489 26.08714925306973 4 11.260382685830965 17.81199497601605 39.763775589667965 31.163846748485017 5 12.018086829349109 39.16047062318439 36.63097098166466 12.190471565801838 6 30.457664760027708 38.65007105265037 19.013117961681555 11.879146225640365 7 26.559790104131608 32.50808090500407 23.516328655454842 17.41580033540947 8 23.632038859977488 38.108296983253666 21.51017754495906 16.74948661180979 9 26.016474963368342 15.672637222334886 20.718951634785938 37.59193617951084 10 13.600965590138411 29.96671856475596 36.00051421960034 20.43180162550529 11 33.39902603430301 23.019722091312854 25.460491552775515 18.120760321608614 12 22.388202514363403 25.457393922169842 33.00776759204646 19.146635971420288 13 28.434881210120132 22.856126861536527 26.108781711099816 22.60021021724353 14 19.79066549179614 23.03793527453554 28.66374662784072 28.507652605827605 15 22.136789731344646 32.23464357323614 23.64072844844822 21.987838246970988 16 21.045160018794657 28.97233500761926 28.932060742731707 21.05044423085437 17 20.742919981148514 29.487296668142417 28.653261164507793 21.116522186201284 18 20.496807482102337 28.48731347226844 30.571625649250034 20.444253396379196 19 22.876925977342452 27.686513445421358 27.896321433264205 21.540239143971984 20 22.633158910964614 28.386812069418582 28.72727843979572 20.252750579821083 21 22.169176083824006 27.68822770836398 27.656809415205597 22.48578679260642 22 22.005446914010452 29.21668120986043 28.01504685002992 20.7628250260992 23 21.33437492859265 28.763738774764068 28.374140255466944 21.527746041176336 24 22.41327356155717 27.941258377736105 28.794848468836175 20.850619591870544 25 21.834807619254352 28.27472844481639 28.61076281086392 21.279701125065337 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 277.0 1 277.0 2 336.5 3 396.0 4 396.0 5 396.0 6 619.0 7 842.0 8 842.0 9 842.0 10 1288.0 11 1734.0 12 1734.0 13 1734.0 14 3023.0 15 4312.0 16 4312.0 17 4312.0 18 7144.0 19 9976.0 20 9976.0 21 9976.0 22 15533.0 23 21090.0 24 21090.0 25 21090.0 26 29855.5 27 38621.0 28 38621.0 29 38621.0 30 49016.5 31 59412.0 32 59412.0 33 59412.0 34 67426.0 35 75440.0 36 75440.0 37 75440.0 38 80520.5 39 85601.0 40 85601.0 41 85601.0 42 88211.5 43 90822.0 44 90822.0 45 90822.0 46 90932.5 47 91043.0 48 91043.0 49 91043.0 50 85451.0 51 79859.0 52 79859.0 53 79859.0 54 69440.5 55 59022.0 56 59022.0 57 59022.0 58 49147.0 59 39272.0 60 39272.0 61 39272.0 62 31171.5 63 23071.0 64 23071.0 65 23071.0 66 17416.0 67 11761.0 68 11761.0 69 11761.0 70 8385.5 71 5010.0 72 5010.0 73 5010.0 74 3448.0 75 1886.0 76 1886.0 77 1886.0 78 1254.0 79 622.0 80 622.0 81 622.0 82 403.5 83 185.0 84 185.0 85 185.0 86 121.0 87 57.0 88 57.0 89 57.0 90 35.5 91 14.0 92 14.0 93 14.0 94 8.5 95 3.0 96 3.0 97 3.0 98 4.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.24059982893851922 2 0.13964785323553225 3 0.14336037285120076 4 0.06996671583375337 5 0.022132328478024026 6 0.021132803966113264 7 0.2972871476854582 8 0.29000489767010834 9 0.01699191670248296 10 0.034697779484902186 11 0.014707289246686933 12 0.016563549054521207 13 0.014992867678661438 14 0.0162779706225467 15 0.014992867678661438 16 0.019133754942291737 17 0.01756307356643197 18 0.014564500030699682 19 0.02413137750184555 20 0.01670633827050846 21 0.014850078462674185 22 0.017134705918470213 23 0.017848651998406472 24 0.0162779706225467 25 0.01527844611063594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 700333.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.77306566425199 #Duplication Level Percentage of deduplicated Percentage of total 1 94.36389861948166 82.82608669862582 2 4.366612714514835 7.665419690429365 3 0.6139009295654702 1.6165189980628603 4 0.21662401769123124 0.760550165170661 5 0.11602213832723679 0.5091809382951742 6 0.07346213307524112 0.3868797978149494 7 0.04913113333480047 0.3018673134648193 8 0.032833587875243805 0.23055237316534105 9 0.0212314509158923 0.1677194581849162 >10 0.12301252138327953 2.024527310470237 >50 0.011717000178913688 0.7127579598999616 >100 0.010089193335863634 1.7042734033617957 >500 0.0011391024713536962 0.7764356529505232 >1k 3.254578489581989E-4 0.31723024010358936 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1155 0.1649215444652758 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1066 0.1522133042424104 No Hit GTCCTACAGTGTGCATTTCTCATTT 962 0.1373632257797362 No Hit CTGTAGGACGTGGAATATGGCAAGA 944 0.13479301989196568 No Hit GTATCAACGCAGAGTACTTTTTTTT 935 0.1335079169480804 No Hit CTTTAGGACGTGAAATATGGCGAGG 828 0.11822947083744448 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 1.4278921598725178E-4 0.0 11 0.0 0.0 0.0 1.4278921598725178E-4 0.0 12 0.0 0.0 0.0 1.4278921598725178E-4 0.0 13 0.0 0.0 0.0 1.4278921598725178E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGAAG 25 0.006095745 18.963728 11 CAATACT 55 1.920882E-4 13.849156 4 GTAGCAC 85 3.7761474E-6 12.33937 3 TAACAGG 70 1.0695679E-4 12.238155 6 GTCCTAT 95 9.817304E-7 12.054557 1 CCTACAC 80 2.755458E-5 11.91871 3 TAACACT 80 2.7962422E-5 11.901618 4 TACTGTG 120 9.686119E-9 11.898207 7 TAGGACC 395 0.0 11.811227 4 GTAGGAC 675 0.0 11.300703 3 TAGGACA 240 0.0 11.108178 4 GGACCTT 60 0.0057854685 11.104993 6 ACTTAGT 60 0.005822623 11.095449 8 CAGTGCG 60 0.0059542777 11.062175 9 GTGTAGA 130 3.041896E-8 11.011374 1 GTCCTAC 565 0.0 10.978892 1 GGTATCA 340 0.0 10.665918 1 TGTAGGA 745 0.0 10.62285 2 GGACGTG 485 0.0 10.59799 6 AGGACGT 505 0.0 10.55524 5 >>END_MODULE