FastQCFastQC Report
Thu 26 May 2016
SRR1926829_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926829_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700333
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17280.24673976522597107No Hit
GTCCTACAGTGGACATTTCTAAATT12530.17891488763202648No Hit
GTCCTAAAGTGTGTATTTCTCATTT10740.15335561797030842No Hit
CTGTAGGACGTGGAATATGGCAAGA10620.1516421473784614No Hit
CTTTAGGACGTGAAATATGGCGAGG10240.14621615717094583No Hit
GGTATCAACGCAGAGTACTTTTTTT9600.1370776473477617No Hit
GTCCTACAGTGTGCATTTCTCATTT9060.1293670296844501No Hit
TATCAACGCAGAGTACTTTTTTTTT8830.1260828777167433No Hit
CTGTAGGACCTGGAATATGGCGAGA8370.11951457378132974No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC508.802703E-515.17972310
GTGCGCC456.82449E-414.75806211
AAGGCGT602.5089332E-514.2830016
AAAGGCG707.0776878E-613.6028595
GGCGTGC707.3387273E-613.5552598
AGGCGTG751.4918443E-512.6533817
GCGTGCG604.1258585E-412.6524789
GCGCCAC604.1333225E-412.64976813
CTAGATT701.0659705E-412.2425734
GGACTGC701.0659705E-412.2425736
GTCTAAT550.002990305212.1310821
CCCTTAT550.002990305212.1310821
GACGTGG2500.011.7676467
TAGCACC657.859685E-411.7193854
TAGGACC1950.011.7193854
CGCCACC658.1040093E-411.67670914
GGACGTG5200.011.536276
CTTATAC752.0253305E-411.427223
AGGACGT5300.011.3186045
GTAGGAC5900.011.1366983
Produced by FastQC (version 0.11.5)