##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926829_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 700333 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.00973965242249 31.0 30.0 33.0 26.0 34.0 2 30.29580213983919 31.0 30.0 34.0 26.0 34.0 3 30.374116313239558 31.0 30.0 34.0 26.0 34.0 4 33.96428127762079 37.0 35.0 37.0 30.0 37.0 5 33.9379238162417 37.0 35.0 37.0 30.0 37.0 6 33.92953637769461 37.0 35.0 37.0 30.0 37.0 7 33.93263490368153 37.0 35.0 37.0 30.0 37.0 8 33.97088813464452 37.0 35.0 37.0 30.0 37.0 9 35.51466659432013 39.0 35.0 39.0 30.0 39.0 10 35.42215774495847 39.0 35.0 39.0 30.0 39.0 11 35.454802215517475 39.0 35.0 39.0 30.0 39.0 12 35.34727194063395 39.0 35.0 39.0 30.0 39.0 13 35.35382025407913 39.0 35.0 39.0 30.0 39.0 14 36.49150332770268 40.0 37.0 41.0 30.0 41.0 15 36.48414111572637 40.0 37.0 41.0 30.0 41.0 16 36.474327212911575 40.0 37.0 41.0 30.0 41.0 17 36.45459088747781 40.0 37.0 41.0 30.0 41.0 18 36.43237002968588 40.0 37.0 41.0 30.0 41.0 19 36.50291218606006 40.0 37.0 41.0 30.0 41.0 20 36.470210599814656 40.0 37.0 41.0 30.0 41.0 21 36.415797913278396 40.0 37.0 41.0 30.0 41.0 22 36.32267221450367 40.0 37.0 41.0 30.0 41.0 23 36.254871611076446 40.0 37.0 41.0 30.0 41.0 24 36.19354364280992 40.0 37.0 41.0 29.0 41.0 25 36.07753311638892 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.549034314839318 1101 2 -0.3999725583406928 1101 3 -0.20921750912322423 1101 4 -0.3684607147998662 1101 5 -0.12643273755519147 1101 6 -0.2692068846186615 1101 7 -0.1644281879661662 1101 8 -0.2169086734595993 1101 9 -0.1424331475219489 1101 10 -0.14049399012642283 1101 11 -0.07746859500338132 1101 12 -0.037835472298951345 1101 13 0.09379229000047928 1101 14 0.030660934324856726 1101 15 0.11996979067943414 1101 16 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2214 7 0.3702301081785322 2214 8 0.37192799663569787 2214 9 0.3420913182321712 2214 10 0.24675197771044566 2214 11 0.3121219416414718 2214 12 0.46045846421422 2214 13 0.20252816312098787 2214 14 0.20702641428358248 2214 15 0.1369395004416205 2214 16 0.2112564522983149 2214 17 0.14361923843267732 2214 18 0.02678024582541383 2214 19 0.13412727385868806 2214 20 0.24736538580695822 2214 21 0.15064386797131135 2214 22 0.39207436700017695 2214 23 0.4485469836122107 2214 24 0.3273219203172957 2214 25 0.35681620887129384 2215 1 0.459249481900855 2215 2 0.4339424304736248 2215 3 0.20187708979016605 2215 4 0.4259264696041001 2215 5 0.3263612860945386 2215 6 0.33965217578402473 2215 7 0.026089549330094997 2215 8 -0.012009344600542704 2215 9 -0.06019840798855114 2215 10 0.15144432882532044 2215 11 -0.28724088647030044 2215 12 0.02568002786084378 2215 13 0.11195943605353165 2215 14 0.12933942585569724 2215 15 -0.013680736645589775 2215 16 0.008017671603987253 2215 17 0.07794537447557559 2215 18 -0.05346883687286663 2215 19 -0.09635677920368835 2215 20 0.12632036746092723 2215 21 0.029485245335123977 2215 22 0.04937608870494614 2215 23 0.11700379986962162 2215 24 -0.01011669102901891 2215 25 0.18859577421140017 2216 1 -0.21062393582066363 2216 2 -0.257871915517935 2216 3 -1.9175110958638406 2216 4 -1.451657918581553 2216 5 -0.619640823610105 2216 6 -0.22136048244382778 2216 7 -0.16416361522686884 2216 8 -0.22420343742754767 2216 9 -0.29970262739783493 2216 10 -0.3125852070396604 2216 11 -0.2222303379470958 2216 12 -0.3128009847973843 2216 13 -0.26856799010680277 2216 14 -0.475491797772996 2216 15 -0.5360330573206937 2216 16 -0.3370245224888393 2216 17 -0.3285208702501592 2216 18 -0.3571502714720225 2216 19 -0.2900487623260517 2216 20 -0.2800614890791593 2216 21 -0.26235019770285106 2216 22 -0.3471639956832391 2216 23 -0.4096101241810146 2216 24 -0.22549643786446438 2216 25 -0.2126911456198215 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15711.0 3 12.0 4 236.0 5 1757.0 6 2164.0 7 1692.0 8 1297.0 9 1170.0 10 993.0 11 905.0 12 847.0 13 825.0 14 805.0 15 817.0 16 814.0 17 892.0 18 1007.0 19 1159.0 20 1578.0 21 2032.0 22 2570.0 23 3014.0 24 3678.0 25 4311.0 26 5310.0 27 5811.0 28 6544.0 29 8051.0 30 10588.0 31 13550.0 32 17739.0 33 23657.0 34 32262.0 35 45654.0 36 67422.0 37 119251.0 38 294208.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22863195935533 19.915771536726297 10.52012362656999 28.335472877348376 2 13.821084745568864 21.758100994736104 40.47709590811486 23.94371835158017 3 17.638977156689826 25.86299726508665 29.85668482801465 26.64134075020887 4 11.61490319605102 18.30386401897383 39.04985199897763 31.03138078599752 5 12.235461446629614 39.05374452358784 36.12932615140316 12.581467878379382 6 30.22270234336048 39.298711123082306 18.39629669958107 12.082289833976148 7 26.936513604227663 32.371920302792255 22.983075055345285 17.708491037634793 8 24.201962444787497 38.56928035598766 20.59595943728588 16.632797761938967 9 26.04652956188066 15.77380994883205 20.368072226355707 37.811588262931586 10 13.840088305138234 30.241225533664906 35.169549006924186 20.749137154272677 11 33.47132457310299 23.205233290398493 25.22555070356968 18.09789143292883 12 22.678341179645567 25.609249367083187 32.514289712406594 19.198119740864648 13 28.463376080397417 22.891788194270912 25.9690913166962 22.675744408635467 14 20.006339841189835 23.168549818443513 28.355368089180434 28.46974225118622 15 22.038511241369953 32.134111078586066 23.716756031214526 22.110621648829458 16 21.01078640518844 29.127863309373065 28.670076507226632 21.191273778211862 17 20.749759756458015 29.61542470631744 28.33046088206862 21.30435465515592 18 20.58563987160302 28.45937903382415 30.50427799545356 20.45070309911927 19 22.602201819142405 27.69237359530507 28.119029600331274 21.58639498522125 20 22.602823835824562 28.317036114712618 28.764971641697272 20.315168407765547 21 22.05973917662986 27.418585521618176 27.86128835091768 22.66038695083428 22 22.037547905850367 29.212287886316773 27.858215817640776 20.89194839019208 23 21.246321392823127 28.85398803140792 28.39392117749699 21.505769398271966 24 22.14295811551966 27.76872650218254 29.12894045815479 20.959374924143013 25 21.676367437397186 28.31677823980991 28.702476695302504 21.304377627490403 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 244.0 1 244.0 2 239.5 3 235.0 4 235.0 5 235.0 6 492.5 7 750.0 8 750.0 9 750.0 10 1175.5 11 1601.0 12 1601.0 13 1601.0 14 2718.0 15 3835.0 16 3835.0 17 3835.0 18 6732.5 19 9630.0 20 9630.0 21 9630.0 22 14977.5 23 20325.0 24 20325.0 25 20325.0 26 28746.5 27 37168.0 28 37168.0 29 37168.0 30 48080.0 31 58992.0 32 58992.0 33 58992.0 34 66517.0 35 74042.0 36 74042.0 37 74042.0 38 79398.0 39 84754.0 40 84754.0 41 84754.0 42 88066.5 43 91379.0 44 91379.0 45 91379.0 46 91525.5 47 91672.0 48 91672.0 49 91672.0 50 86776.5 51 81881.0 52 81881.0 53 81881.0 54 70743.5 55 59606.0 56 59606.0 57 59606.0 58 50085.0 59 40564.0 60 40564.0 61 40564.0 62 32090.5 63 23617.0 64 23617.0 65 23617.0 66 17882.5 67 12148.0 68 12148.0 69 12148.0 70 8584.5 71 5021.0 72 5021.0 73 5021.0 74 3471.5 75 1922.0 76 1922.0 77 1922.0 78 1296.5 79 671.0 80 671.0 81 671.0 82 430.0 83 189.0 84 189.0 85 189.0 86 131.0 87 73.0 88 73.0 89 73.0 90 42.5 91 12.0 92 12.0 93 12.0 94 7.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11523089730171218 2 0.12151362280515127 3 0.01827701964636823 4 0.0 5 0.00813898531127335 6 0.3371253389459014 7 0.026130426525667078 8 0.01199429414292915 9 0.01285102943885266 10 0.00485483334356656 11 0.006711093151400833 12 5.711568639490071E-4 13 0.0017134705918470212 14 0.0 15 0.002570205887770532 16 9.995245119107626E-4 17 2.8557843197450357E-4 18 7.139460799362589E-4 19 4.283676479617553E-4 20 0.001285102943885266 21 0.012708240222865408 22 1.4278921598725178E-4 23 0.0 24 2.8557843197450357E-4 25 0.00642551471942633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 700333.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.48857194187173 #Duplication Level Percentage of deduplicated Percentage of total 1 92.97054059562491 79.47918748183785 2 5.518694869660751 9.435706867804631 3 0.7656995412494798 1.9637568095389297 4 0.25924693359135603 0.8865060053213712 5 0.12513347838056835 0.5348741184436934 6 0.07007358740875269 0.35942945510509144 7 0.05079821263531627 0.3039866658774913 8 0.03620020920800927 0.2475763351351766 9 0.030246920834809468 0.2327189461026037 >10 0.1466417744967085 2.3246891868385773 >50 0.013696236051474563 0.8293133874605242 >100 0.011023388423855572 1.786369359465498 >500 0.001169147253106938 0.7390494594336524 >1k 8.351051807906698E-4 0.8768359216348646 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1728 0.24673976522597107 No Hit GTCCTACAGTGGACATTTCTAAATT 1253 0.17891488763202648 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1074 0.15335561797030842 No Hit CTGTAGGACGTGGAATATGGCAAGA 1062 0.1516421473784614 No Hit CTTTAGGACGTGAAATATGGCGAGG 1024 0.14621615717094583 No Hit GGTATCAACGCAGAGTACTTTTTTT 960 0.1370776473477617 No Hit GTCCTACAGTGTGCATTTCTCATTT 906 0.1293670296844501 No Hit TATCAACGCAGAGTACTTTTTTTTT 883 0.1260828777167433 No Hit CTGTAGGACCTGGAATATGGCGAGA 837 0.11951457378132974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.4278921598725178E-4 0.0 10 0.0 0.0 0.0 1.4278921598725178E-4 0.0 11 0.0 0.0 0.0 1.4278921598725178E-4 0.0 12 0.0 0.0 0.0 1.4278921598725178E-4 0.0 13 0.0 0.0 0.0 1.4278921598725178E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 50 8.802703E-5 15.179723 10 GTGCGCC 45 6.82449E-4 14.758062 11 AAGGCGT 60 2.5089332E-5 14.283001 6 AAAGGCG 70 7.0776878E-6 13.602859 5 GGCGTGC 70 7.3387273E-6 13.555259 8 AGGCGTG 75 1.4918443E-5 12.653381 7 GCGTGCG 60 4.1258585E-4 12.652478 9 GCGCCAC 60 4.1333225E-4 12.649768 13 CTAGATT 70 1.0659705E-4 12.242573 4 GGACTGC 70 1.0659705E-4 12.242573 6 GTCTAAT 55 0.0029903052 12.131082 1 CCCTTAT 55 0.0029903052 12.131082 1 GACGTGG 250 0.0 11.767646 7 TAGCACC 65 7.859685E-4 11.719385 4 TAGGACC 195 0.0 11.719385 4 CGCCACC 65 8.1040093E-4 11.676709 14 GGACGTG 520 0.0 11.53627 6 CTTATAC 75 2.0253305E-4 11.42722 3 AGGACGT 530 0.0 11.318604 5 GTAGGAC 590 0.0 11.136698 3 >>END_MODULE