FastQCFastQC Report
Thu 26 May 2016
SRR1926828_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926828_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85798
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC6730.7844005687778269No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6620.7715797571038953No Hit
CCCATGTACTCTGCGTTGATACCAC6310.7354483787500874No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA2800.32634793351826386No Hit
GAGTACATGGGAAGCAGTGGTATCA2760.32168582018228864No Hit
GTATCAACGCAGAGTACTTTTTTTT2610.30420289517238164No Hit
CATGTACTCTGCGTTGATACCACTG2290.26690598848458No Hit
GGTATCAACGCAGAGTACTTTTTTT2050.2389333084687289No Hit
GCGTTGATACCACTGCTTCCCATGT1930.22494696846080325No Hit
GTATCAACGCAGAGTACATGGGAAG1680.1958087601109583No Hit
GGTATCAACGCAGAGTACATGGGAA1620.1888155901069955No Hit
TATCAACGCAGAGTACATGGGAAGC1560.1818224201030327No Hit
TATCAACGCAGAGTACTTTTTTTTT1560.1818224201030327No Hit
ACGCAGAGTACATGGGAAGCAGTGG1450.16900160842910092No Hit
ACTCTGCGTTGATACCACTGCTTCC1190.13869787174526213No Hit
GTACTCTGCGTTGATACCACTGCTT970.11305624839739853No Hit
GTGGTATCAACGCAGAGTACATGGG960.11189072006340475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCATGT1854.9112714E-1110.3178821
AGTGGTA1252.5418594E-69.8563514
GGAAGCA1553.718742E-89.82307158
CCATGTA1701.4253601E-89.5496472
GGGAAGC1502.4253313E-79.5216757
CAGTGGT1304.192945E-69.4772613
CATGGGA1951.4642865E-99.28296854
GAAAAAA850.0070427678.9826261
TTGATAC2252.4738256E-108.84544216
TGATACC2303.8198777E-108.65315117
GAAGCAG1651.0301701E-68.615699
CTCTGCG2451.4551915E-108.5101929
AAGCAGT1451.6718403E-58.49685310
TCTGCGT2355.838956E-108.46904110
TGGTATC1606.358521E-68.29260316
TGCGTTG2408.8220986E-108.29260212
GTGGTAT1502.5600024E-58.21362515
CTGCGTT2451.3224053E-98.12336511
GATACCA2501.9626896E-97.96089818
ATACCAC2501.9626896E-97.96089819
Produced by FastQC (version 0.11.5)