##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926828_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85798 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.300426583370243 31.0 28.0 33.0 16.0 34.0 2 28.46974288444952 31.0 29.0 33.0 16.0 34.0 3 28.275507587589455 31.0 28.0 33.0 16.0 34.0 4 31.869309307909276 35.0 32.0 37.0 19.0 37.0 5 31.76448168954987 35.0 32.0 37.0 19.0 37.0 6 31.409263619198583 35.0 32.0 37.0 17.0 37.0 7 31.275309447772674 35.0 32.0 37.0 16.0 37.0 8 31.059418634467004 35.0 32.0 37.0 11.0 37.0 9 33.13475838597636 37.0 33.0 39.0 17.0 39.0 10 33.3468728874799 38.0 34.0 39.0 17.0 39.0 11 33.50079255926712 38.0 34.0 39.0 18.0 39.0 12 33.420534278188306 38.0 34.0 39.0 17.0 39.0 13 33.49321662509615 38.0 34.0 39.0 17.0 39.0 14 34.582041539429824 39.0 34.0 41.0 18.0 41.0 15 34.10745005711089 39.0 34.0 41.0 12.0 41.0 16 34.17479428424905 39.0 34.0 41.0 17.0 41.0 17 34.40886733956503 39.0 34.0 41.0 17.0 41.0 18 34.47213221753421 39.0 35.0 41.0 17.0 41.0 19 33.79933098673629 39.0 33.0 41.0 10.0 41.0 20 34.03653931327071 39.0 34.0 41.0 10.0 41.0 21 34.02275111307956 39.0 34.0 41.0 10.0 41.0 22 34.08154036224621 39.0 34.0 41.0 8.0 41.0 23 34.104501270425885 39.0 34.0 41.0 2.0 41.0 24 32.770367607636544 39.0 32.0 41.0 2.0 41.0 25 33.367153080491384 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.483227956498201 1101 2 -0.8903331095696494 1101 3 -1.9063584785177667 1101 4 -1.6430956872646867 1101 5 -1.6100997253084195 1101 6 -3.3170202550663888 1101 7 -1.759679225610224 1101 8 -1.7180126298438871 1101 9 -1.164899010369659 1101 10 -11.769676625496764 1101 11 -5.215899053855214 1101 12 -2.852654963428108 1101 13 -1.7951025718817135 1101 14 -2.220793506991612 1101 15 -2.312463395250113 1101 16 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23 1.4098166645467316 2210 24 0.9111544937719884 2210 25 0.7147836250252908 2211 1 -0.1722170844186266 2211 2 0.08185055917890693 2211 3 -0.04653890877453293 2211 4 -0.11510586538173584 2211 5 0.39568329574177596 2211 6 -1.7044248352190614 2211 7 -0.4535839214233164 2211 8 -0.4026644919766653 2211 9 -0.3096480272560811 2211 10 0.27814895858836053 2211 11 0.5539945385574612 2211 12 0.392140317626712 2211 13 0.729243946739615 2211 14 0.5940919891915968 2211 15 1.2563545552472277 2211 16 -0.35442759848905325 2211 17 -0.6013967230750978 2211 18 -0.5190535412124149 2211 19 -0.4382207469064028 2211 20 -0.7858728744610275 2211 21 -0.4902658689419752 2211 22 -2.9332898060351766 2211 23 -1.593487049283297 2211 24 0.4702226186295917 2211 25 -0.4125802391806772 2212 1 -0.5876875788364906 2212 2 0.12964048854778198 2212 3 -3.898897076922175 2212 4 -1.8356572438278498 2212 5 -0.786590570742618 2212 6 0.18467290914184176 2212 7 0.2163294984901043 2212 8 0.35906938817826983 2212 9 0.6079300087430113 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2213 22 1.4950318722865035 2213 23 1.3074453516491005 2213 24 2.0372389356459024 2213 25 1.8065339799335405 2214 1 -0.9399969236270387 2214 2 -1.3375990388421215 2214 3 -1.4373861567646387 2214 4 -1.4042215116402375 2214 5 -1.3637477494035544 2214 6 -1.1755132705932105 2214 7 -1.7836705015098957 2214 8 -5.459405370146115 2214 9 -3.5778916883568854 2214 10 -3.1097607507499205 2214 11 -2.9300809098036993 2214 12 -3.1043037145316035 2214 13 -3.043947147880054 2214 14 -2.3361801196519423 2214 15 -4.078834065655677 2214 16 -3.1198883282354934 2214 17 -2.9223614726112714 2214 18 -2.5886268250714117 2214 19 -2.1809542039255767 2214 20 -3.114722596167894 2214 21 -2.788812560345807 2214 22 -2.911180962497763 2214 23 -3.0792631779165696 2214 24 -4.960667919403807 2214 25 -4.514002626317353 2215 1 0.9123124211635094 2215 2 0.8655427441868824 2215 3 0.8003510433785763 2215 4 1.150245011811247 2215 5 1.1335222112122736 2215 6 1.762680427938836 2215 7 0.5725325060089013 2215 8 -0.217058431370603 2215 9 0.5703360237806052 2215 10 0.747247842160931 2215 11 0.5350551446180702 2215 12 -0.21730944313883782 2215 13 0.5436378861335527 2215 14 0.17303935761265166 2215 15 0.7057165967145309 2215 16 1.1914256710484281 2215 17 0.21060487182123921 2215 18 0.39342652258343946 2215 19 0.7202720760601053 2215 20 -3.5169334805216366 2215 21 -0.7809357254012994 2215 22 0.014078615017453444 2215 23 0.8195911006369556 2215 24 0.8181093809900375 2215 25 0.9347084370553631 2216 1 -0.5774438487057942 2216 2 -1.3406123246933745 2216 3 -0.5108464133504924 2216 4 -1.0356875208627194 2216 5 -0.7393583963468942 2216 6 -1.9510691305210734 2216 7 -9.885300415220346 2216 8 -11.394071854189395 2216 9 -6.11740682326991 2216 10 -3.416431069884286 2216 11 -2.8114213654490428 2216 12 -2.210818803788783 2216 13 -1.8967983554771841 2216 14 -2.159263715506391 2216 15 -2.557352990003942 2216 16 -1.9069494614135962 2216 17 -2.547298695822704 2216 18 -2.050983579853863 2216 19 -1.7219303259179952 2216 20 -2.2157589838773397 2216 21 -3.8943232209859033 2216 22 -3.351075040934507 2216 23 -3.4024152575403654 2216 24 -2.7381869807196075 2216 25 -3.1711728769679475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4828.0 3 0.0 4 39.0 5 239.0 6 379.0 7 305.0 8 217.0 9 202.0 10 187.0 11 173.0 12 178.0 13 169.0 14 173.0 15 161.0 16 205.0 17 213.0 18 209.0 19 252.0 20 323.0 21 444.0 22 556.0 23 627.0 24 768.0 25 809.0 26 992.0 27 1048.0 28 1122.0 29 1350.0 30 1644.0 31 2015.0 32 2642.0 33 3482.0 34 4555.0 35 6663.0 36 9913.0 37 15722.0 38 22994.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.031123676893245 19.119330794214548 11.871159193401407 24.978386335490804 2 15.437769192160891 22.10848225228485 37.43419747178224 25.01955108377202 3 18.840901132251663 26.71296836698961 28.837399322983543 25.608731177775184 4 12.751936176168705 19.172809554912757 34.1711766352524 33.904077633666134 5 13.050270471926877 39.82465957843686 33.15496176086551 13.970108188770752 6 31.644049617608655 36.53003171050177 17.451268420070882 14.374650251818691 7 26.566061555365934 34.13833007983729 21.360857520251553 17.93475084454523 8 25.762890213960016 35.509178066175615 20.293464281538643 18.434467438325733 9 26.568274286247217 17.379545110108534 19.255295585166532 36.79688501847772 10 16.113838010516375 30.36690722971634 31.39755861538282 22.121696144384465 11 32.05954698592928 26.057052260990194 21.80086499341346 20.08253575966706 12 22.872197806040965 28.213706998053183 28.469008288548746 20.44508690735711 13 28.429875383234442 23.95696116946248 24.1271580616206 23.48600538568248 14 21.535071869061913 24.69777689698185 25.753954838484045 28.01319639547219 15 24.974354191923904 30.32150517579036 22.103189405949827 22.600951226335912 16 21.79179295873164 29.473070645838188 26.14712054091863 22.588015854511543 17 22.41224542137353 29.921076254094825 25.837326152088508 21.82935217244314 18 22.711959246004454 28.56626604338855 26.992527656994973 21.72924705361202 19 23.188034088391994 29.301561024517063 26.626018629702603 20.884386257388343 20 23.515422815975377 28.44303000629503 26.414632440372106 21.626914737357488 21 22.732041687067216 28.373085262642512 26.49040591266233 22.40446713762794 22 23.1449854862965 28.50164954943402 25.053333488767908 23.300031475501566 23 22.118209372814178 28.717649801818606 25.990906971321987 23.173233854045233 24 23.143549928889513 29.670560257396655 25.63125131146394 21.55463850224989 25 23.097816583705395 27.87148968909924 25.461921359709965 23.568772367485398 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 788.0 1 788.0 2 731.0 3 674.0 4 674.0 5 674.0 6 600.0 7 526.0 8 526.0 9 526.0 10 480.5 11 435.0 12 435.0 13 435.0 14 503.0 15 571.0 16 571.0 17 571.0 18 815.0 19 1059.0 20 1059.0 21 1059.0 22 1508.5 23 1958.0 24 1958.0 25 1958.0 26 2553.5 27 3149.0 28 3149.0 29 3149.0 30 4032.0 31 4915.0 32 4915.0 33 4915.0 34 5694.0 35 6473.0 36 6473.0 37 6473.0 38 7126.5 39 7780.0 40 7780.0 41 7780.0 42 8425.5 43 9071.0 44 9071.0 45 9071.0 46 10809.0 47 12547.0 48 12547.0 49 12547.0 50 12038.0 51 11529.0 52 11529.0 53 11529.0 54 10172.5 55 8816.0 56 8816.0 57 8816.0 58 7563.5 59 6311.0 60 6311.0 61 6311.0 62 5311.0 63 4311.0 64 4311.0 65 4311.0 66 3485.5 67 2660.0 68 2660.0 69 2660.0 70 1988.5 71 1317.0 72 1317.0 73 1317.0 74 949.0 75 581.0 76 581.0 77 581.0 78 415.0 79 249.0 80 249.0 81 249.0 82 152.5 83 56.0 84 56.0 85 56.0 86 37.5 87 19.0 88 19.0 89 19.0 90 11.0 91 3.0 92 3.0 93 3.0 94 1.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23776778013473507 2 0.14569104174922493 3 0.1491876267512063 4 0.07226275670761556 5 0.025641623347863587 6 0.025641623347863587 7 0.29021655516445605 8 0.31236159351033826 9 0.02214503834588219 10 0.031469265017832586 11 0.01981398167789459 12 0.01981398167789459 13 0.017482925009906992 14 0.01981398167789459 15 0.01631739667591319 16 0.02097951001188839 17 0.02214503834588219 18 0.017482925009906992 19 0.024476095013869787 20 0.01864845334390079 21 0.01864845334390079 22 0.01981398167789459 23 0.02097951001188839 24 0.01864845334390079 25 0.017482925009906992 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 85798.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.86556796195715 #Duplication Level Percentage of deduplicated Percentage of total 1 92.1586002222316 76.36774749994171 2 6.043855577591178 10.016550502342712 3 1.0169205451706824 2.5280309564325507 4 0.3052168164620167 1.011678593906618 5 0.14065291081198925 0.5827641669968997 6 0.06329380986539516 0.3146926501783258 7 0.06048075164915538 0.3508240285321336 8 0.03516322770299731 0.23310566679875985 9 0.01125223286495914 0.08391804004755356 >10 0.1153353868658312 1.9184596377537937 >50 0.028130582162397853 1.5618079675516912 >100 0.01687834929743871 2.7389915848854285 >500 0.0042195873243596775 2.2914287046318096 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 673 0.7844005687778269 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 662 0.7715797571038953 No Hit CCCATGTACTCTGCGTTGATACCAC 631 0.7354483787500874 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 280 0.32634793351826386 No Hit GAGTACATGGGAAGCAGTGGTATCA 276 0.32168582018228864 No Hit GTATCAACGCAGAGTACTTTTTTTT 261 0.30420289517238164 No Hit CATGTACTCTGCGTTGATACCACTG 229 0.26690598848458 No Hit GGTATCAACGCAGAGTACTTTTTTT 205 0.2389333084687289 No Hit GCGTTGATACCACTGCTTCCCATGT 193 0.22494696846080325 No Hit GTATCAACGCAGAGTACATGGGAAG 168 0.1958087601109583 No Hit GGTATCAACGCAGAGTACATGGGAA 162 0.1888155901069955 No Hit TATCAACGCAGAGTACATGGGAAGC 156 0.1818224201030327 No Hit TATCAACGCAGAGTACTTTTTTTTT 156 0.1818224201030327 No Hit ACGCAGAGTACATGGGAAGCAGTGG 145 0.16900160842910092 No Hit ACTCTGCGTTGATACCACTGCTTCC 119 0.13869787174526213 No Hit GTACTCTGCGTTGATACCACTGCTT 97 0.11305624839739853 No Hit GTGGTATCAACGCAGAGTACATGGG 96 0.11189072006340475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCATGT 185 4.9112714E-11 10.317882 1 AGTGGTA 125 2.5418594E-6 9.85635 14 GGAAGCA 155 3.718742E-8 9.8230715 8 CCATGTA 170 1.4253601E-8 9.549647 2 GGGAAGC 150 2.4253313E-7 9.521675 7 CAGTGGT 130 4.192945E-6 9.47726 13 CATGGGA 195 1.4642865E-9 9.2829685 4 GAAAAAA 85 0.007042767 8.982626 1 TTGATAC 225 2.4738256E-10 8.845442 16 TGATACC 230 3.8198777E-10 8.653151 17 GAAGCAG 165 1.0301701E-6 8.61569 9 CTCTGCG 245 1.4551915E-10 8.510192 9 AAGCAGT 145 1.6718403E-5 8.496853 10 TCTGCGT 235 5.838956E-10 8.469041 10 TGGTATC 160 6.358521E-6 8.292603 16 TGCGTTG 240 8.8220986E-10 8.292602 12 GTGGTAT 150 2.5600024E-5 8.213625 15 CTGCGTT 245 1.3224053E-9 8.123365 11 GATACCA 250 1.9626896E-9 7.960898 18 ATACCAC 250 1.9626896E-9 7.960898 19 >>END_MODULE