FastQCFastQC Report
Thu 26 May 2016
SRR1926828_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926828_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85798
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8460.9860369705587543No Hit
GTACATGGGAAGCAGTGGTATCAAC7360.8578288538194364No Hit
CCCATGTACTCTGCGTTGATACCAC7120.8298561738035852No Hit
GGTATCAACGCAGAGTACTTTTTTT6290.7331173220820999No Hit
TATCAACGCAGAGTACTTTTTTTTT5040.5874262803328749No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3090.360148255204084No Hit
GAGTACATGGGAAGCAGTGGTATCA2970.3461619151961584No Hit
CATGTACTCTGCGTTGATACCACTG2390.27856127182451806No Hit
GCGTTGATACCACTGCTTCCCATGT2230.25991281848061726No Hit
ACGCAGAGTACATGGGAAGCAGTGG2010.23427119513275366No Hit
TATCAACGCAGAGTACATGGGAAGC1880.2191193267908343No Hit
GTATCAACGCAGAGTACATGGGAAG1880.2191193267908343No Hit
GAGTACTTTTTTTTTTTTTTTTTTT1750.2039674584489149No Hit
ACTCTGCGTTGATACCACTGCTTCC1730.2016364017809273No Hit
GTACTTTTTTTTTTTTTTTTTTTTT1710.1993053451129397No Hit
GGTATCAACGCAGAGTACATGGGAA1620.1888155901069955No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1420.1655050234271195No Hit
GTGGTATCAACGCAGAGTACATGGG1070.12471153173733653No Hit
GCTTCCCATGTACTCTGCGTTGATA1030.12004941840136135No Hit
ATCAACGCAGAGTACTTTTTTTTTT970.11305624839739853No Hit
GGGAAGCAGTGGTATCAACGCAGAG970.11305624839739853No Hit
GCAGAGTACATGGGAAGCAGTGGTA940.10955966339541715No Hit
GTACTCTGCGTTGATACCACTGCTT930.10839413506142333No Hit
CAGTGGTATCAACGCAGAGTACATG900.10489755005944194No Hit
CATGGGAAGCAGTGGTATCAACGCA890.10373202172544815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAAGA250.006013335718.98542419
TGTAGGA350.002117066916.3208032
ATATGGC456.709572E-414.76643915
AATATGG550.003045458812.08163314
CCATGTA1050.00433897468.1604012
ATGTACT1150.0099116087.44209674
GTATCAA5200.07.32343671
GTACATG5000.06.85473731
AACGCAG5650.06.732286
TACATGG5000.06.47391842
TCAACGC6000.06.3395644
CATGGGG1650.0050322326.3395644
CAACGCA6000.06.3395645
ACATGGG5000.06.27616833
ATCAACG6100.06.23563673
AGTGGTA2556.8747766E-55.95621114
GTGGTAT2556.8747766E-55.95621115
CAGTGGT2556.8747766E-55.95621113
CGCAGAG6400.05.9364068
ACGCAGA6450.05.89038757
Produced by FastQC (version 0.11.5)