##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926828_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 85798 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.95692207277559 31.0 30.0 33.0 26.0 34.0 2 30.218664770740578 31.0 30.0 34.0 26.0 34.0 3 30.24227837478729 31.0 30.0 34.0 26.0 34.0 4 33.869146133942515 37.0 35.0 37.0 30.0 37.0 5 33.926082193058114 37.0 35.0 37.0 30.0 37.0 6 33.86492692137346 37.0 35.0 37.0 30.0 37.0 7 33.83668616984079 37.0 35.0 37.0 30.0 37.0 8 33.847047716729996 37.0 35.0 37.0 30.0 37.0 9 35.38556842816849 39.0 35.0 39.0 30.0 39.0 10 35.27440033567216 39.0 35.0 39.0 29.0 39.0 11 35.32405184270029 39.0 35.0 39.0 30.0 39.0 12 35.225226695260965 39.0 35.0 39.0 29.0 39.0 13 35.22579780414462 39.0 35.0 39.0 30.0 39.0 14 36.334984498473155 40.0 37.0 41.0 30.0 41.0 15 36.27993659525863 40.0 36.0 41.0 29.0 41.0 16 36.22435254901047 40.0 36.0 41.0 29.0 41.0 17 36.24436467050514 40.0 37.0 41.0 29.0 41.0 18 36.25250005827642 40.0 37.0 41.0 29.0 41.0 19 36.288153570013286 40.0 37.0 41.0 29.0 41.0 20 36.2896454462808 40.0 37.0 41.0 29.0 41.0 21 36.21918925849087 40.0 37.0 41.0 29.0 41.0 22 36.127520455022264 40.0 37.0 41.0 29.0 41.0 23 36.08896477773375 40.0 37.0 41.0 29.0 41.0 24 35.98672463227581 40.0 37.0 41.0 28.0 41.0 25 35.8909415137882 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.414477094102665 1101 2 -0.7051323555755644 1101 3 0.10276165307409713 1101 4 -0.315228881623149 1101 5 -0.8075537384095739 1101 6 -0.11178521152640286 1101 7 -0.047706973979025236 1101 8 -0.11001669920320012 1101 9 -0.8203717371121968 1101 10 -0.6340573507851985 1101 11 -0.7633231245202055 1101 12 -0.6709641455670194 1101 13 -0.9590086542486986 1101 14 -1.1657630437265922 1101 15 -1.2108666177540997 1101 16 -0.7385947597075102 1101 17 -1.2215545387893485 1101 18 -0.6008387286101851 1101 19 -1.0518594397803227 1101 20 -0.8825100361410989 1101 21 -0.27119084480558087 1101 22 -0.6005290863718784 1101 23 -0.4965499325531795 1101 24 -0.8652510402719287 1101 25 -0.6964883966594826 1102 1 -1.3586603002095359 1102 2 -1.3326132716060997 1102 3 -1.203681095017508 1102 4 -1.3699464388750542 1102 5 -2.0431567918446873 1102 6 -1.2220752878622818 1102 7 -1.771798577032456 1102 8 -1.4402762411879309 1102 9 -1.6267228821503608 1102 10 -1.5792787248309992 1102 11 -1.5064070939858496 1102 12 -1.3096817028189278 1102 13 -1.6283827000502242 1102 14 -1.662068387238044 1102 15 -1.8148360834029589 1102 16 -1.8275107902418597 1102 17 -2.1706232410794257 1102 18 -1.7384265148697224 1102 19 -2.794332722223068 1102 20 -1.8641894254540787 1102 21 -1.984290081446801 1102 22 -2.3992466436237834 1102 23 -3.006901077591344 1102 24 -2.1099533303482687 1102 25 -2.248793740170939 1103 1 -0.5217714113206462 1103 2 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2213 7 1.14825736702349 2213 8 0.9288985839868928 2213 9 0.8690533416258646 2213 10 0.500945051392776 2213 11 0.6896208780421205 2213 12 0.3757448706076474 2213 13 0.37206485239732956 2213 14 0.6179875568179014 2213 15 0.5093597207928511 2213 16 0.5427269719958971 2213 17 0.8222718638156792 2213 18 0.6816574012141885 2213 19 0.8265903547000093 2213 20 1.0435028822382293 2213 21 1.2901714570147362 2213 22 0.9007253843482417 2213 23 0.9830849363946683 2213 24 0.9611515647566264 2213 25 0.8634859801087842 2214 1 0.22539412155917304 2214 2 0.08507380322383185 2214 3 -0.5810416392351954 2214 4 0.26689709853990706 2214 5 0.2437003510124569 2214 6 0.30837369172955675 2214 7 0.07494972228727192 2214 8 0.022091105750838835 2214 9 0.3391546688700444 2214 10 0.5002314792506368 2214 11 0.07813712370122516 2214 12 -0.1840762606420583 2214 13 0.3094132183171254 2214 14 0.014067667183724097 2214 15 -0.2880333623145219 2214 16 -0.015429157588798148 2214 17 -0.2077933091066413 2214 18 -0.01673944003979244 2214 19 -0.48162523922987077 2214 20 -0.05330507171257892 2214 21 -0.3979499453923836 2214 22 -0.6510561674333104 2214 23 -0.07171740412195504 2214 24 -0.6142254391918058 2214 25 -0.36699782180358653 2215 1 0.8459186471588858 2215 2 0.7752814652360058 2215 3 0.728213641824599 2215 4 1.3620009295459994 2215 5 0.7718958397342561 2215 6 1.0017141858219318 2215 7 0.07920142296754307 2215 8 0.406355337759436 2215 9 0.4479086967970076 2215 10 0.8431423278005852 2215 11 0.368908695487832 2215 12 0.6575814550758139 2215 13 0.7472488788971461 2215 14 0.6805105601303723 2215 15 0.38937444291283185 2215 16 0.7081207887055072 2215 17 0.9147451799732025 2215 18 0.9300471693408028 2215 19 0.8483514883032441 2215 20 1.0318632061248678 2215 21 0.6859204448689837 2215 22 0.9319638826920027 2215 23 1.469098922408655 2215 24 1.1055203538622607 2215 25 1.0839957335132766 2216 1 0.2973933910656328 2216 2 0.10228605725296092 2216 3 -1.9917616319302596 2216 4 -1.3587249623649882 2216 5 -1.127344711307778 2216 6 -0.43823636168778535 2216 7 -0.4937851542136684 2216 8 -0.21171248279867427 2216 9 -0.9184812714121051 2216 10 -0.4497753691263 2216 11 -0.3830782349254491 2216 12 -0.4325810316779837 2216 13 -0.28105384098982 2216 14 0.03634772014450505 2216 15 0.07429143337557065 2216 16 0.19659659230847382 2216 17 -0.08799236859620407 2216 18 -0.30154060882945544 2216 19 -0.5657689638338113 2216 20 -0.7608337207560893 2216 21 -0.02681357137969087 2216 22 -0.22791778456338108 2216 23 -0.802713158128256 2216 24 -1.148718431019411 2216 25 -0.50780045158033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2063.0 3 0.0 4 25.0 5 228.0 6 321.0 7 240.0 8 189.0 9 157.0 10 131.0 11 130.0 12 105.0 13 107.0 14 125.0 15 104.0 16 121.0 17 132.0 18 123.0 19 136.0 20 204.0 21 241.0 22 290.0 23 401.0 24 495.0 25 515.0 26 614.0 27 709.0 28 828.0 29 988.0 30 1255.0 31 1687.0 32 2158.0 33 2877.0 34 4046.0 35 5583.0 36 8433.0 37 14539.0 38 35498.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51479082793628 18.99760779508723 10.684403990897952 26.803197386078537 2 15.545105543821983 21.67936615363073 36.384321870733615 26.391206431813675 3 18.749417140725544 25.60733936398396 29.377273151170378 26.265970344120117 4 12.971164829016994 17.9701158535164 34.9238909997902 34.1348283176764 5 13.654641878897372 38.44279969695204 33.48097208462031 14.421586339530275 6 32.36721756318936 35.4515363112156 17.325754120027604 14.855492005567447 7 26.869360442071393 33.182167921844766 21.399426426356406 18.549045209727435 8 27.146736913267983 34.376930518806894 19.792990104087746 18.683342463837377 9 27.250474980476262 15.29961651883021 19.70557038453021 37.74433811616332 10 16.895892582404773 28.536295398386873 31.714765257121545 22.85304676208681 11 32.40981409173029 24.030537910134623 22.585232239641005 20.974415758494086 12 23.788433296813448 26.57521154339262 29.289727033264178 20.346628126529755 13 29.22096086155854 21.966712510781136 25.02039674584489 23.791929881815427 14 22.408447749364786 22.57744935779389 26.717406000139864 28.296696892701462 15 25.741593332944813 27.523748470190572 23.758960312372515 22.975697884492103 16 22.07510664584256 27.567076155621344 27.46800624723187 22.889810951304227 17 22.534324809436118 28.021632205878927 27.075223198675957 22.368819786009 18 22.942259726333948 25.592671157835845 28.927247721392106 22.537821394438097 19 23.08795076808317 26.691764376791998 29.010000233105664 21.210284622019163 20 23.578638196694563 25.78381780461083 28.689479941257375 21.948064057437236 21 22.742638658988646 25.726808570162962 29.066514349660782 22.464038421187606 22 23.52968600666682 25.577519289493928 27.396909018858253 23.495885684981 23 22.128720949206276 26.048392736427424 28.68015571458542 23.14273059978088 24 22.892142007972215 26.805986153523392 28.521643861162264 21.78022797734213 25 23.086157862837428 24.982516667443704 27.719240990255955 24.212084479462913 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 108.0 1 108.0 2 104.0 3 100.0 4 100.0 5 100.0 6 196.0 7 292.0 8 292.0 9 292.0 10 324.0 11 356.0 12 356.0 13 356.0 14 368.0 15 380.0 16 380.0 17 380.0 18 680.5 19 981.0 20 981.0 21 981.0 22 1417.0 23 1853.0 24 1853.0 25 1853.0 26 2673.0 27 3493.0 28 3493.0 29 3493.0 30 4435.0 31 5377.0 32 5377.0 33 5377.0 34 6006.0 35 6635.0 36 6635.0 37 6635.0 38 7214.0 39 7793.0 40 7793.0 41 7793.0 42 8448.5 43 9104.0 44 9104.0 45 9104.0 46 10887.0 47 12670.0 48 12670.0 49 12670.0 50 12249.5 51 11829.0 52 11829.0 53 11829.0 54 10333.5 55 8838.0 56 8838.0 57 8838.0 58 7646.5 59 6455.0 60 6455.0 61 6455.0 62 5448.0 63 4441.0 64 4441.0 65 4441.0 66 3586.5 67 2732.0 68 2732.0 69 2732.0 70 2066.0 71 1400.0 72 1400.0 73 1400.0 74 1007.5 75 615.0 76 615.0 77 615.0 78 433.5 79 252.0 80 252.0 81 252.0 82 158.5 83 65.0 84 65.0 85 65.0 86 44.0 87 23.0 88 23.0 89 23.0 90 14.5 91 6.0 92 6.0 93 6.0 94 3.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12004941840136135 2 0.11538730506538615 3 0.01631739667591319 4 0.0 5 0.0034965850019813985 6 0.3508240285321336 7 0.023310566679875987 8 0.005827641669968997 9 0.005827641669968997 10 0.0023310566679875986 11 0.0034965850019813985 12 0.0 13 0.0 14 0.0 15 0.0034965850019813985 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.013986340007925594 22 0.0 23 0.0 24 0.0 25 0.0023310566679875986 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 85798.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.35732767663582 #Duplication Level Percentage of deduplicated Percentage of total 1 90.56501241059233 71.86997365905965 2 7.270110300057279 11.538730506538613 3 1.1999353767973329 2.8567099466188024 4 0.3877392160030549 1.2307979206974522 5 0.18065122563778696 0.7167999254061866 6 0.10280964060687063 0.4895219002773957 7 0.06462320266717582 0.3589827268700902 8 0.03524901963664135 0.2237814401268095 9 0.027905473879007738 0.1993053451129397 >10 0.1086844772129775 1.7214853493088416 >50 0.029374183030534465 1.678360800951071 >100 0.020561928121374123 3.121284878435395 >500 0.007343545757633616 3.9942656005967505 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 846 0.9860369705587543 No Hit GTACATGGGAAGCAGTGGTATCAAC 736 0.8578288538194364 No Hit CCCATGTACTCTGCGTTGATACCAC 712 0.8298561738035852 No Hit GGTATCAACGCAGAGTACTTTTTTT 629 0.7331173220820999 No Hit TATCAACGCAGAGTACTTTTTTTTT 504 0.5874262803328749 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 309 0.360148255204084 No Hit GAGTACATGGGAAGCAGTGGTATCA 297 0.3461619151961584 No Hit CATGTACTCTGCGTTGATACCACTG 239 0.27856127182451806 No Hit GCGTTGATACCACTGCTTCCCATGT 223 0.25991281848061726 No Hit ACGCAGAGTACATGGGAAGCAGTGG 201 0.23427119513275366 No Hit TATCAACGCAGAGTACATGGGAAGC 188 0.2191193267908343 No Hit GTATCAACGCAGAGTACATGGGAAG 188 0.2191193267908343 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 175 0.2039674584489149 No Hit ACTCTGCGTTGATACCACTGCTTCC 173 0.2016364017809273 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 171 0.1993053451129397 No Hit GGTATCAACGCAGAGTACATGGGAA 162 0.1888155901069955 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 142 0.1655050234271195 No Hit GTGGTATCAACGCAGAGTACATGGG 107 0.12471153173733653 No Hit GCTTCCCATGTACTCTGCGTTGATA 103 0.12004941840136135 No Hit ATCAACGCAGAGTACTTTTTTTTTT 97 0.11305624839739853 No Hit GGGAAGCAGTGGTATCAACGCAGAG 97 0.11305624839739853 No Hit GCAGAGTACATGGGAAGCAGTGGTA 94 0.10955966339541715 No Hit GTACTCTGCGTTGATACCACTGCTT 93 0.10839413506142333 No Hit CAGTGGTATCAACGCAGAGTACATG 90 0.10489755005944194 No Hit CATGGGAAGCAGTGGTATCAACGCA 89 0.10373202172544815 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0011655283339937993 0.0 0.0 0.0 0.0 7 0.0011655283339937993 0.0 0.0 0.0 0.0 8 0.0011655283339937993 0.0 0.0 0.0 0.0 9 0.0011655283339937993 0.0 0.0 0.0 0.0 10 0.0011655283339937993 0.0 0.0 0.0 0.0 11 0.0011655283339937993 0.0 0.0 0.0011655283339937993 0.0 12 0.0011655283339937993 0.0 0.0 0.0011655283339937993 0.0 13 0.0011655283339937993 0.0 0.0 0.0011655283339937993 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCAAGA 25 0.0060133357 18.985424 19 TGTAGGA 35 0.0021170669 16.320803 2 ATATGGC 45 6.709572E-4 14.766439 15 AATATGG 55 0.0030454588 12.081633 14 CCATGTA 105 0.0043389746 8.160401 2 ATGTACT 115 0.009911608 7.4420967 4 GTATCAA 520 0.0 7.3234367 1 GTACATG 500 0.0 6.8547373 1 AACGCAG 565 0.0 6.73228 6 TACATGG 500 0.0 6.4739184 2 TCAACGC 600 0.0 6.339564 4 CATGGGG 165 0.005032232 6.339564 4 CAACGCA 600 0.0 6.339564 5 ACATGGG 500 0.0 6.2761683 3 ATCAACG 610 0.0 6.2356367 3 AGTGGTA 255 6.8747766E-5 5.956211 14 GTGGTAT 255 6.8747766E-5 5.956211 15 CAGTGGT 255 6.8747766E-5 5.956211 13 CGCAGAG 640 0.0 5.936406 8 ACGCAGA 645 0.0 5.8903875 7 >>END_MODULE