FastQCFastQC Report
Thu 26 May 2016
SRR1926827_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926827_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740597
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18000.24304716330203874No Hit
CTGTAGGACGTGGAATATGGCAAGA14320.19335752102695528No Hit
GTCCTAAAGTGTGTATTTCTCATTT13740.18552600132055624No Hit
GTATCAACGCAGAGTACTTTTTTTT12730.17188835493527518No Hit
GTCCTACAGTGTGCATTTCTCATTT12180.16446191383437955No Hit
CTTTAGGACGTGAAATATGGCGAGG10550.1424526429353616No Hit
CTGTAGGACCTGGAATATGGCGAGA8570.11571745497213734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGG250.00609505518.96427314
TCGAACT400.0053419314.22320519
TAGGACC3700.013.8988694
TCCCTAT852.54935E-713.4720721
CCCTATA603.9810344E-412.706352
GGCGAGG3800.012.47649619
GTAGGAC8600.012.0784213
AGGACGT7650.012.0695635
TGTAGGA9800.012.0580682
CCTATAC959.98838E-712.0375963
AGGACCT6650.012.0237225
CTGTAGG8900.012.0088131
GGACCTG6300.011.9362486
TAGGACG7900.011.9342614
GTCCTAC8900.011.9015911
TGGCGAG8900.011.826035518
GGACGTG7400.011.7055266
GCCTCGA658.142253E-411.67032216
TCCTACA9500.011.6363422
ACGTGGA3850.011.6085228
Produced by FastQC (version 0.11.5)