##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926827_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 740597 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.533709966418982 31.0 30.0 33.0 22.0 34.0 2 28.68128820397598 31.0 30.0 33.0 22.0 34.0 3 28.470460992955683 31.0 28.0 33.0 16.0 34.0 4 32.09980596734796 35.0 33.0 37.0 20.0 37.0 5 31.900520796060476 35.0 32.0 37.0 19.0 37.0 6 31.64061156067335 35.0 32.0 37.0 17.0 37.0 7 31.444350976306954 35.0 32.0 37.0 17.0 37.0 8 31.233569674195277 35.0 32.0 37.0 17.0 37.0 9 33.43678275769413 37.0 33.0 39.0 18.0 39.0 10 33.63318646983447 38.0 34.0 39.0 19.0 39.0 11 33.78097669852835 38.0 34.0 39.0 22.0 39.0 12 33.68835142459394 38.0 34.0 39.0 19.0 39.0 13 33.786585686952556 38.0 35.0 39.0 22.0 39.0 14 34.84125374528927 39.0 35.0 41.0 22.0 41.0 15 34.45955222610948 39.0 34.0 41.0 17.0 41.0 16 34.537428588017505 39.0 34.0 41.0 18.0 41.0 17 34.76157883437281 39.0 35.0 41.0 22.0 41.0 18 34.80730680788607 39.0 35.0 41.0 22.0 41.0 19 34.224281221771086 39.0 34.0 41.0 16.0 41.0 20 34.51626863192803 39.0 34.0 41.0 18.0 41.0 21 34.486098377390135 39.0 34.0 41.0 18.0 41.0 22 34.50887594737759 39.0 35.0 41.0 18.0 41.0 23 34.50743926859007 39.0 35.0 41.0 16.0 41.0 24 33.27154849398526 39.0 33.0 41.0 2.0 41.0 25 33.83326019414067 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.4342913028439135 1101 2 -0.45282291907143346 1101 3 -1.158888726880427 1101 4 -0.6563367520627814 1101 5 -0.7106534479716906 1101 6 -2.608529975719261 1101 7 -1.1758093468133346 1101 8 -0.5601606066595082 1101 9 -0.47973384307869793 1101 10 -9.230812942993932 1101 11 -4.238545389860391 1101 12 -1.9502394581977853 1101 13 -1.1464028849409544 1101 14 -1.1540564257131791 1101 15 -2.254336855063414 1101 16 -0.7511049941201691 1101 17 -0.8918110538630444 1101 18 -0.6232740588544701 1101 19 -1.2676443434592315 1101 20 -0.5374572887231466 1101 21 -0.8592374107053473 1101 22 -0.49647959801053076 1101 23 -0.6576725390417977 1101 24 -0.7895181120127788 1101 25 -0.7261423046427709 1102 1 -0.2202489922702462 1102 2 -0.3248710571277442 1102 3 -0.555178264593323 1102 4 -0.41517244887457494 1102 5 -0.39753647401967385 1102 6 -1.2105084359113896 1102 7 -0.13622025395150317 1102 8 0.40195495517213686 1102 9 0.18091639525805192 1102 10 0.08785638217164404 1102 11 -0.2696526447116554 1102 12 -0.092611687823144 1102 13 -0.09948303105895206 1102 14 -0.49748177890901246 1102 15 0.10988329649091355 1102 16 0.039199274726335886 1102 17 -0.09112637437459625 1102 18 -0.5018043597753348 1102 19 0.025193289217099846 1102 20 -0.009977293761700423 1102 21 -0.28677839865923005 1102 22 -0.23437795849238086 1102 23 -0.941999328887789 1102 24 -3.857444913792179 1102 25 -2.0257488335448315 1103 1 0.09593541316432308 1103 2 -0.11033937668697291 1103 3 0.19005525842719706 1103 4 0.10726157953158832 1103 5 -0.243414799410278 1103 6 0.47003371418948703 1103 7 0.36260676876403863 1103 8 0.3611881296508308 1103 9 0.3441177518900673 1103 10 0.42339072359516905 1103 11 0.28943943735653477 1103 12 0.22928679266856733 1103 13 0.04020683374704248 1103 14 0.2781326936198596 1103 15 0.625226677212531 1103 16 0.5041900919898836 1103 17 0.32299241806205004 1103 18 0.015581677103071456 1103 19 -1.4082503039809708 1103 20 -0.43159560025060983 1103 21 -0.1712702984132406 1103 22 -0.19195053227264935 1103 23 -0.12369015888651802 1103 24 1.3566024945982917 1103 25 0.6168466635629457 1104 1 -0.37547689170252596 1104 2 -0.39587105712774573 1104 3 -1.7571109491026498 1104 4 -0.5977742331405906 1104 5 -0.6383385826976955 1104 6 -1.4681848349381532 1104 7 -0.6724051687933539 1104 8 -0.11544731571188649 1104 9 0.040845024617340187 1104 10 0.11864875848957013 1104 11 -0.03494623757459436 1104 12 -0.18580227987504827 1104 13 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24 -4.767354466315187 1105 25 -2.5679708794561797 1106 1 -0.2142491886631639 1106 2 -0.7536827163205686 1106 3 -0.3621772171644331 1106 4 -0.686368154416126 1106 5 -0.07862956426221857 1106 6 0.26532787059248975 1106 7 -2.180195901229826 1106 8 -0.15364960768996383 1106 9 -0.2637728129511743 1106 10 0.15902344707950533 1106 11 -0.38754813094778484 1106 12 0.0030447555011363647 1106 13 -0.043790909688127044 1106 14 -0.32364949845992896 1106 15 0.4234041305061709 1106 16 0.25007371418821833 1106 17 -0.1615340533376397 1106 18 -0.4323917382572162 1106 19 0.20512684294692463 1106 20 0.12951077536860822 1106 21 -0.12610935345612262 1106 22 -0.525840594739968 1106 23 -0.41306083260289483 1106 24 -2.9717120408911306 1106 25 -1.3070996894906273 1107 1 0.16540425425178995 1107 2 -0.20858185852147315 1107 3 0.0896594728872877 1107 4 0.033758093611247375 1107 5 0.06542990355859502 1107 6 -0.13481665839924517 1107 7 -2.749905749513445 1107 8 -1.8464840946511032 1107 9 -0.8139524976435979 1107 10 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1108 22 0.9147226558152042 1108 23 0.8540530937202533 1108 24 -0.1285097241953892 1108 25 0.7637968202448846 1109 1 0.6135916662170935 1109 2 0.8397034109573625 1109 3 -0.2692433368095024 1109 4 0.7195969252518424 1109 5 1.124744159619091 1109 6 1.2540846686079483 1109 7 1.2926444765967169 1109 8 1.5909167504819663 1109 9 0.9210530624423967 1109 10 1.1185731083713648 1109 11 0.9112287033237507 1109 12 0.3394554033400823 1109 13 0.5483396122086361 1109 14 0.615844824600849 1109 15 -0.6582025864101553 1109 16 0.4805822534497395 1109 17 0.37512847886348766 1109 18 0.6212062008707306 1109 19 -0.8448874686645595 1109 20 0.33680015615288283 1109 21 0.5096620424030505 1109 22 0.7138197430140636 1109 23 0.5212278803061849 1109 24 -0.3603886938822569 1109 25 0.01399180941214695 1110 1 0.3231634319403369 1110 2 0.4491551918138299 1110 3 0.5348736214119825 1110 4 0.30551149458543847 1110 5 0.6273488761413262 1110 6 0.590703113128459 1110 7 0.721536688389822 1110 8 0.9837356742213146 1110 9 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0.14211816236175778 2215 4 0.6491284342326651 2215 5 0.8052982305861747 2215 6 1.1758321231964537 2215 7 0.977104105186509 2215 8 -0.32425424028351557 2215 9 0.18140958918190364 2215 10 -0.09966320052827626 2215 11 -0.2840640612806524 2215 12 -0.46323241589342246 2215 13 0.18148374507742915 2215 14 0.14226907112522014 2215 15 0.07299384155723487 2215 16 0.1550577802907256 2215 17 -0.22558119200888882 2215 18 -0.8494320970016105 2215 19 0.1628553179267911 2215 20 -3.669180292380755 2215 21 -2.0009173981138133 2215 22 -0.9919252760976605 2215 23 -0.8225883765013151 2215 24 -0.5543351810452606 2215 25 -0.37941819633827834 2216 1 -0.633295267770901 2216 2 -1.3513133648200508 2216 3 -0.44094000893170815 2216 4 -0.6564109852773434 2216 5 -0.3361184972276092 2216 6 -1.8776079118612294 2216 7 -9.968161579049763 2216 8 -11.307173811438382 2216 9 -6.26590070187839 2216 10 -3.552520044929235 2216 11 -2.320636408514762 2216 12 -2.0202915961143653 2216 13 -1.663766907158518 2216 14 -1.9160843898017745 2216 15 -2.9978043321821133 2216 16 -2.087403289376226 2216 17 -2.1464440797835422 2216 18 -2.081803891873406 2216 19 -1.4940316312395723 2216 20 -2.234149107349566 2216 21 -4.696900287111408 2216 22 -3.2727166726978822 2216 23 -3.0588812304412265 2216 24 -3.3472388029930045 2216 25 -2.8467821949522794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39433.0 3 3.0 4 293.0 5 1864.0 6 2588.0 7 2004.0 8 1524.0 9 1347.0 10 1247.0 11 1209.0 12 1233.0 13 1183.0 14 1258.0 15 1324.0 16 1417.0 17 1460.0 18 1661.0 19 2086.0 20 2744.0 21 3547.0 22 4283.0 23 5357.0 24 6229.0 25 7028.0 26 8076.0 27 8736.0 28 9800.0 29 11994.0 30 14316.0 31 17484.0 32 22530.0 33 29897.0 34 40960.0 35 57991.0 36 84335.0 37 137264.0 38 204892.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68834886412083 19.317186728113096 12.510709137787524 25.48375526997855 2 13.514682935667674 20.53420519602037 43.58583277219342 22.365279096118535 3 18.34244476686404 25.380672222319877 30.32221075874826 25.954672252067816 4 10.776683286716121 17.810612509627468 40.51805910251733 30.89464510113908 5 11.549905255580331 39.71567219916452 36.78068861567801 11.953733929577142 6 30.065003734372027 39.0331690569799 19.342426382899415 11.559400825748664 7 26.54880919169129 32.52126878709003 23.453222211087123 17.476699810131553 8 23.17567100938824 38.924841261136095 21.532830406363367 16.366657323112296 9 25.977381261074378 15.721833268507716 20.918552011755047 37.38223345866286 10 13.037505504391355 30.585640224876336 36.5483481422848 19.828506128447504 11 33.399192404823964 23.08506759220495 25.610220535605766 17.905519467365323 12 22.215214575784543 25.774753835786086 33.519658699107715 18.49037288932165 13 28.3776921871877 23.20358796233774 26.284173963774656 22.134545886699907 14 19.19528183907798 23.66872935399442 29.223804726264014 27.91218408066359 15 21.794599967318366 33.10919853013666 23.80181963788207 21.294381864662913 16 20.156502385081225 29.74193583240145 29.93398484136374 20.16757694115359 17 20.109825254676554 29.96573700551962 29.22470014815335 20.69973759165047 18 19.517527867134472 29.104713441857825 31.693716761335246 19.684041929672453 19 22.277206995611873 28.023911173179076 28.479741547554248 21.2191402836548 20 21.977152318327978 28.97366398863423 29.656879626290905 19.392304066746885 21 21.581618459327842 28.081303799417135 28.17138026312591 22.16569747812911 22 21.215231448219235 30.015746797971012 28.92670581308038 19.842315940729378 23 20.7470190948096 29.377835219315486 29.06114147499605 20.81400421087887 24 21.879064114250987 28.18245045410041 29.90107701137783 20.037408420270772 25 21.17399683178051 28.77947922898556 29.555193327562296 20.491330611671636 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 214.0 1 214.0 2 308.5 3 403.0 4 403.0 5 403.0 6 667.5 7 932.0 8 932.0 9 932.0 10 1445.0 11 1958.0 12 1958.0 13 1958.0 14 3310.5 15 4663.0 16 4663.0 17 4663.0 18 8046.5 19 11430.0 20 11430.0 21 11430.0 22 17565.5 23 23701.0 24 23701.0 25 23701.0 26 33611.0 27 43521.0 28 43521.0 29 43521.0 30 56024.0 31 68527.0 32 68527.0 33 68527.0 34 76895.5 35 85264.0 36 85264.0 37 85264.0 38 90112.0 39 94960.0 40 94960.0 41 94960.0 42 97149.5 43 99339.0 44 99339.0 45 99339.0 46 98294.0 47 97249.0 48 97249.0 49 97249.0 50 90153.5 51 83058.0 52 83058.0 53 83058.0 54 69932.5 55 56807.0 56 56807.0 57 56807.0 58 46127.5 59 35448.0 60 35448.0 61 35448.0 62 27285.5 63 19123.0 64 19123.0 65 19123.0 66 13988.0 67 8853.0 68 8853.0 69 8853.0 70 6144.0 71 3435.0 72 3435.0 73 3435.0 74 2316.0 75 1197.0 76 1197.0 77 1197.0 78 778.0 79 359.0 80 359.0 81 359.0 82 236.0 83 113.0 84 113.0 85 113.0 86 70.0 87 27.0 88 27.0 89 27.0 90 19.5 91 12.0 92 12.0 93 12.0 94 6.0 95 0.0 96 0.0 97 0.0 98 2.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23521564359563976 2 0.13921201409133444 3 0.14177751192618926 4 0.07115880836676357 5 0.02484482113754174 6 0.024034663926534944 7 0.29584240821931496 8 0.2862555478890679 9 0.02011890407333543 10 0.03767231031181601 11 0.01714832763297718 12 0.018363563449487374 13 0.016338170421970385 14 0.01876864205499077 15 0.016203144220135916 16 0.021739218495349023 17 0.019983877871500962 18 0.01606811801830145 19 0.02565497834854854 20 0.017283353834811645 21 0.015528013210963589 22 0.01741838003664611 23 0.018093511045818442 24 0.01647319662380485 25 0.015933091816466984 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 740597.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.26483905982965 #Duplication Level Percentage of deduplicated Percentage of total 1 95.07532632152711 82.9673305000885 2 3.8327252796678444 6.689243093815101 3 0.46050705918219165 1.205582232163482 4 0.17823715818096342 0.622153476925727 5 0.10996880705330746 0.4798205124554168 6 0.07043694859339016 0.36880013897206126 7 0.0461506617854274 0.28191310512469664 8 0.03654991943180035 0.25516182694926387 9 0.02474374105623041 0.19433327229090416 >10 0.1371255487844709 2.3520214563797395 >50 0.015200417665006481 0.9327081212243524 >100 0.011011877784928436 1.939494327994644 >500 0.001085678077809383 0.6081052308993983 >1k 9.305812095508996E-4 1.103332704716647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1800 0.24304716330203874 No Hit CTGTAGGACGTGGAATATGGCAAGA 1432 0.19335752102695528 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1374 0.18552600132055624 No Hit GTATCAACGCAGAGTACTTTTTTTT 1273 0.17188835493527518 No Hit GTCCTACAGTGTGCATTTCTCATTT 1218 0.16446191383437955 No Hit CTTTAGGACGTGAAATATGGCGAGG 1055 0.1424526429353616 No Hit CTGTAGGACCTGGAATATGGCGAGA 857 0.11571745497213734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 1.3502620183446597E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTGG 25 0.006095055 18.964273 14 TCGAACT 40 0.00534193 14.223205 19 TAGGACC 370 0.0 13.898869 4 TCCCTAT 85 2.54935E-7 13.472072 1 CCCTATA 60 3.9810344E-4 12.70635 2 GGCGAGG 380 0.0 12.476496 19 GTAGGAC 860 0.0 12.078421 3 AGGACGT 765 0.0 12.069563 5 TGTAGGA 980 0.0 12.058068 2 CCTATAC 95 9.98838E-7 12.037596 3 AGGACCT 665 0.0 12.023722 5 CTGTAGG 890 0.0 12.008813 1 GGACCTG 630 0.0 11.936248 6 TAGGACG 790 0.0 11.934261 4 GTCCTAC 890 0.0 11.901591 1 TGGCGAG 890 0.0 11.8260355 18 GGACGTG 740 0.0 11.705526 6 GCCTCGA 65 8.142253E-4 11.670322 16 TCCTACA 950 0.0 11.636342 2 ACGTGGA 385 0.0 11.608522 8 >>END_MODULE