FastQCFastQC Report
Thu 26 May 2016
SRR1926827_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926827_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740597
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21090.28477025966888875No Hit
GTCCTACAGTGGACATTTCTAAATT17650.23832124623783243No Hit
CTGTAGGACGTGGAATATGGCAAGA16420.22171302341219312No Hit
GTCCTAAAGTGTGTATTTCTCATTT13790.1862011323297286No Hit
GGTATCAACGCAGAGTACTTTTTTT13070.17647924579764704No Hit
GTCCTACAGTGTGCATTTCTCATTT12890.17404877416462666No Hit
CTTTAGGACGTGAAATATGGCGAGG12650.17080814532059946No Hit
TATCAACGCAGAGTACTTTTTTTTT10970.14812374341240916No Hit
CTGTAGGACCTGGAATATGGCGAGA9380.1266545773207291No Hit
CACACACACACACACACACACACAC7590.10248488719235968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3800.015.2990381
TCTACAC1051.9324943E-812.6919063
TTAATCA604.021737E-412.6910474
TGTAGGA8650.012.2274662
CCAACGA550.003090098712.07819119
GTATTAT959.978485E-712.0385881
GTATAAG1353.5106495E-1012.0014311
TAGGACC3500.011.9658444
CTGTAGG8400.011.79972651
TAGGACA3150.011.7845444
GTCCTAA4550.011.7299061
GTAGGAC9100.011.5063983
GTGTAGG1001.8519404E-611.4366591
GTCCATA752.0101524E-411.4366591
GGACATG2500.011.4211696
GGACCTG5100.011.3838456
GACGTGG3700.011.2854147
GTCTTAA1201.2158125E-711.1189741
GGTCTAC1201.2158125E-711.1189741
TAACACT1303.162495E-810.9826364