##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926827_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 740597 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.914378535154746 31.0 30.0 33.0 26.0 34.0 2 30.18681820207211 31.0 30.0 34.0 26.0 34.0 3 30.259226002805846 31.0 30.0 34.0 26.0 34.0 4 33.84608768331495 37.0 35.0 37.0 30.0 37.0 5 33.79651956462151 37.0 35.0 37.0 30.0 37.0 6 33.706338264940314 37.0 35.0 37.0 29.0 37.0 7 33.68707002593853 37.0 35.0 37.0 29.0 37.0 8 33.72164078439421 37.0 35.0 37.0 29.0 37.0 9 35.279388115263764 39.0 35.0 39.0 29.0 39.0 10 35.15766604509605 39.0 35.0 39.0 29.0 39.0 11 35.20032892382767 39.0 35.0 39.0 29.0 39.0 12 35.10596721293767 39.0 35.0 39.0 29.0 39.0 13 35.10511519760409 39.0 35.0 39.0 29.0 39.0 14 36.20247989122289 40.0 36.0 41.0 29.0 41.0 15 36.19339532836347 40.0 36.0 41.0 29.0 41.0 16 36.17472525543582 40.0 36.0 41.0 29.0 41.0 17 36.159258004015676 40.0 36.0 41.0 29.0 41.0 18 36.122513323710464 40.0 36.0 41.0 28.0 41.0 19 36.20609994369407 40.0 37.0 41.0 29.0 41.0 20 36.16993587605675 40.0 37.0 41.0 28.0 41.0 21 36.11842608058094 40.0 37.0 41.0 28.0 41.0 22 36.032626381149264 40.0 37.0 41.0 27.0 41.0 23 35.959390869798284 40.0 37.0 41.0 27.0 41.0 24 35.90251378279955 40.0 36.0 41.0 27.0 41.0 25 35.786493869135306 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.291012438575855 1101 2 -0.2509974680252931 1101 3 0.06629414123150568 1101 4 -0.14093854036416786 1101 5 -0.18599666726619546 1101 6 -0.36144261462237637 1101 7 -0.19662312604500443 1101 8 -0.05126984026264125 1101 9 -0.020650252613265252 1101 10 0.0755535427069347 1101 11 -0.11602762349588858 1101 12 0.016013732913229717 1101 13 -0.09839293020993267 1101 14 0.019623449843031437 1101 15 -0.0476822444941547 1101 16 0.01391005885987795 1101 17 0.11011774782793537 1101 18 -0.04886737792837437 1101 19 0.0565417109225379 1101 20 -0.07599422314000037 1101 21 -0.21070346888035374 1101 22 -0.1786849022132131 1101 23 -0.13874628017160262 1101 24 -0.05264114443483692 1101 25 -0.047436103571101285 1102 1 -0.7025112750682254 1102 2 -0.6682997545995413 1102 3 -0.6038257564063407 1102 4 -0.7993065137393955 1102 5 -0.5637488103080202 1102 6 -0.7029710526051005 1102 7 -0.6478900565726562 1102 8 -0.5738380761729331 1102 9 -0.5012916071880724 1102 10 -0.46987712085704203 1102 11 -0.557281261528999 1102 12 -0.47434837983099243 1102 13 -0.5326456334591327 1102 14 -0.586934457036584 1102 15 -0.6975832966232787 1102 16 -0.6832663633707483 1102 17 -0.5474853270144422 1102 18 -0.4390219819048511 1102 19 -0.5271728286135158 1102 20 -0.704283369702182 1102 21 -0.7083308746229804 1102 22 -0.6867051492348324 1102 23 -0.6441743250147098 1102 24 -0.5899413426620654 1102 25 -0.6572931015225336 1103 1 -1.2311470019454944 1103 2 -0.9854987853851682 1103 3 -0.8366290715752847 1103 4 -0.976357380737177 1103 5 -0.9204679593297556 1103 6 -0.9803190179922154 1103 7 -0.6807485016739676 1103 8 -0.7860388831813054 1103 9 -0.839822905573385 1103 10 -0.7197640123331865 1103 11 -0.8654064751784318 1103 12 -0.7963356714599499 1103 13 -0.8484923890039653 1103 14 -0.8895500288127351 1103 15 -1.0204092670106277 1103 16 -0.8326031285004731 1103 17 -0.9320853082468048 1103 18 -0.8276209778362968 1103 19 -0.8479603320486646 1103 20 -0.9621758311447479 1103 21 -0.808462080510651 1103 22 -0.9407569479117086 1103 23 -0.9792218808651896 1103 24 -0.9205148280132534 1103 25 -0.8530998752987813 1104 1 -0.24602303500334344 1104 2 -0.18011402872768656 1104 3 0.07124237011434076 1104 4 -0.07290099873534217 1104 5 0.023495309724424374 1104 6 0.06870210230163565 1104 7 0.04108804561712276 1104 8 -0.12742363983878846 1104 9 -0.08273199648247243 1104 10 -0.012267226290944677 1104 11 -0.2483412777495957 1104 12 -0.046833375775840125 1104 13 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9 0.4136350186162474 1107 10 0.498589142272742 1107 11 0.4626931783092516 1107 12 0.4704620171780931 1107 13 0.5404964861268127 1107 14 0.5506358016169983 1107 15 0.36724859126807274 1107 16 0.3280131706967566 1107 17 0.3088023456120226 1107 18 0.4122204199873636 1107 19 0.29631581555342734 1107 20 0.49568356740201125 1107 21 0.5051123716447279 1107 22 0.5499107362149545 1107 23 0.5110114630266906 1107 24 0.39795255087540937 1107 25 0.4091760355358858 1108 1 0.6587057146340207 1108 2 0.5492529570570532 1108 3 0.7365582970072886 1108 4 0.6354622156539804 1108 5 0.7873340100435371 1108 6 0.7089016961513579 1108 7 0.558685956844279 1108 8 0.5855047036483043 1108 9 0.6175708755801921 1108 10 0.5989680507615631 1108 11 0.6222615653023311 1108 12 0.5596116488832834 1108 13 0.6356655924243668 1108 14 0.6047928780628098 1108 15 0.6531000967432163 1108 16 0.7322838135523781 1108 17 0.5695054869589953 1108 18 0.6789400034457884 1108 19 0.5900585944582772 1108 20 0.5795384874506553 1108 21 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0.432964863786534 2213 2 0.45450203287847657 2213 3 0.41664171004833506 2213 4 0.5916380551700513 2213 5 0.5365800181952665 2213 6 0.6274985819495953 2213 7 0.4134972865412152 2213 8 0.4425521577409697 2213 9 0.4372548021973941 2213 10 0.40161176160784606 2213 11 0.47013616999517893 2213 12 0.5015736649282303 2213 13 0.43682932498561655 2213 14 0.366225548745021 2213 15 0.30567788715933375 2213 16 0.395212834914048 2213 17 0.3781638984920548 2213 18 0.43490300183439956 2213 19 0.6755806584140132 2213 20 0.649442148835071 2213 21 0.5837453220477826 2213 22 0.4785281624175042 2213 23 0.575008542177116 2213 24 0.43709366010828177 2213 25 0.47331685728356376 2214 1 0.7891441710376483 2214 2 0.5894631018009022 2214 3 0.5850546678489685 2214 4 0.6005056895062992 2214 5 0.6752018900911949 2214 6 0.8080634830999074 2214 7 0.7013469452935013 2214 8 0.6144576978095415 2214 9 0.7488796540029625 2214 10 0.7409129247338697 2214 11 0.8407504158857861 2214 12 0.720540950006253 2214 13 0.44643392622243994 2214 14 0.6104499711872649 2214 15 0.7442853401376439 2214 16 0.7419397406143275 2214 17 0.5945758309474343 2214 18 0.5032807133919661 2214 19 0.6134030814330202 2214 20 0.6993556353293542 2214 21 0.6718128666663219 2214 22 0.7212626300390212 2214 23 0.57130768665467 2214 24 0.7190315626364026 2214 25 0.6676838668000542 2215 1 0.41875350624378527 2215 2 0.30179093505963195 2215 3 0.14687777022032833 2215 4 0.29891740790659327 2215 5 0.47641458193781006 2215 6 0.38426189739084293 2215 7 0.1613106211338149 2215 8 0.28535360799728693 2215 9 0.18823991660708828 2215 10 0.12648281787086546 2215 11 -0.1487150632998535 2215 12 0.14742700224924477 2215 13 0.21332903378002044 2215 14 0.23260153451529675 2215 15 0.25657432815478387 2215 16 0.05755732850489892 2215 17 0.13493114914398774 2215 18 -0.00535656466279022 2215 19 0.01503586784593125 2215 20 -0.19714366236926395 2215 21 0.04330972045900694 2215 22 -0.014287454597301519 2215 23 0.09877590301494621 2215 24 0.11084370601907523 2215 25 0.009052505570174674 2216 1 5.581615778531557E-4 2216 2 0.03159964648599001 2216 3 -1.645667886295918 2216 4 -1.2449480072823533 2216 5 -0.10724614326703374 2216 6 -0.022126957527426327 2216 7 -0.1412516979726206 2216 8 -0.04565868257382988 2216 9 0.027601336850857194 2216 10 -0.030628337402056616 2216 11 -0.06101862817694581 2216 12 -0.039720127912588055 2216 13 -0.16468755747186492 2216 14 -0.46406925958196865 2216 15 -0.19257674599629127 2216 16 -0.11293485485492738 2216 17 0.1147245737903475 2216 18 0.10218848038225303 2216 19 -0.04819917149205111 2216 20 -0.13097483517102404 2216 21 0.1705059211846205 2216 22 0.05868016800267384 2216 23 0.05188243148912619 2216 24 0.037023845434511315 2216 25 0.05693579610052524 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18826.0 3 15.0 4 321.0 5 2065.0 6 2825.0 7 2127.0 8 1497.0 9 1356.0 10 1191.0 11 1006.0 12 1004.0 13 928.0 14 950.0 15 922.0 16 958.0 17 1065.0 18 1088.0 19 1387.0 20 1744.0 21 2303.0 22 2796.0 23 3401.0 24 4162.0 25 4804.0 26 5818.0 27 6300.0 28 7208.0 29 8864.0 30 11509.0 31 14582.0 32 19244.0 33 25507.0 34 34007.0 35 48273.0 36 71582.0 37 124695.0 38 304267.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.36226367143672 20.094382727230943 10.367906087699424 28.17544751363291 2 13.436629276977966 21.721349706024302 41.60705812052936 23.23496289646837 3 18.027641753795542 25.817651936462322 29.68639006906387 26.46831624067827 4 11.152084061912214 18.354381667762627 39.70377951841555 30.78975475190961 5 11.808051809861265 39.627900193638816 36.336985554058174 12.22706244244174 6 29.678501643671524 39.83267884582551 18.655468561563833 11.833350948939133 7 27.03886484821964 32.45368794671075 22.617272545555718 17.890174659513892 8 23.624678940957548 39.570543862539466 20.490116377994863 16.314660818508127 9 25.95284909441525 15.84165635853918 20.41643934769809 37.78905519934748 10 13.313906698138176 31.059435781377438 35.513608546999315 20.113048973485075 11 33.35736932791038 23.39066863275701 25.24549122551164 18.006470813820965 12 22.3934366020697 26.002441290211074 33.02020545725582 18.583916650463415 13 28.509335137302877 23.37936426276923 26.149996826849982 21.961303773077915 14 19.420465976680912 23.93183858924243 28.708403378364693 27.939292055711963 15 21.410352142439518 33.32032552820016 23.790780481810902 21.478541847549423 16 20.129518531871256 29.947109665414963 29.595497637691082 20.327874165022706 17 19.963785923769507 30.327736039622145 28.96072892643223 20.747749110176127 18 19.585034114646277 29.237190298018746 31.38993047444541 19.78784511288957 19 21.973067528318524 28.11409235572035 28.616392539492004 21.296447576469127 20 22.005803473172104 29.052878039295642 29.53762603110219 19.403692456430065 21 21.511573733344317 28.007881023020527 28.118480026684207 22.362065216950953 22 21.285289145499032 30.172050618690893 28.633830050391847 19.908830185418232 23 20.608509081187204 29.498094105161105 29.03684459969457 20.85655221395712 24 21.501466386531927 28.15435136025579 30.15814290112288 20.1860393520894 25 21.06332758094528 29.10708353925802 29.354868329727964 20.47472055006873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 256.0 2 278.5 3 301.0 4 301.0 5 301.0 6 543.5 7 786.0 8 786.0 9 786.0 10 1354.0 11 1922.0 12 1922.0 13 1922.0 14 3115.5 15 4309.0 16 4309.0 17 4309.0 18 7475.0 19 10641.0 20 10641.0 21 10641.0 22 16731.0 23 22821.0 24 22821.0 25 22821.0 26 32218.0 27 41615.0 28 41615.0 29 41615.0 30 54410.5 31 67206.0 32 67206.0 33 67206.0 34 75794.0 35 84382.0 36 84382.0 37 84382.0 38 89583.0 39 94784.0 40 94784.0 41 94784.0 42 97336.0 43 99888.0 44 99888.0 45 99888.0 46 99085.5 47 98283.0 48 98283.0 49 98283.0 50 91839.0 51 85395.0 52 85395.0 53 85395.0 54 71710.0 55 58025.0 56 58025.0 57 58025.0 58 47049.5 59 36074.0 60 36074.0 61 36074.0 62 27862.5 63 19651.0 64 19651.0 65 19651.0 66 14383.5 67 9116.0 68 9116.0 69 9116.0 70 6280.5 71 3445.0 72 3445.0 73 3445.0 74 2304.5 75 1164.0 76 1164.0 77 1164.0 78 778.5 79 393.0 80 393.0 81 393.0 82 248.5 83 104.0 84 104.0 85 104.0 86 65.0 87 26.0 88 26.0 89 26.0 90 17.5 91 9.0 92 9.0 93 9.0 94 5.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11382708814645481 2 0.12287384366936405 3 0.016608222825639316 4 0.0 5 0.005806126678882037 6 0.35309351779712855 7 0.024979847339376204 8 0.010667069944922811 9 0.010937122348591745 10 0.004455864660537377 11 0.005536074275213105 12 6.751310091723298E-4 13 0.0010802096146757278 14 2.7005240366893195E-4 15 0.0021604192293514556 16 0.0010802096146757278 17 1.3502620183446597E-4 18 8.10157211006796E-4 19 5.401048073378639E-4 20 8.10157211006796E-4 21 0.011342200954095142 22 1.3502620183446597E-4 23 0.0 24 1.3502620183446597E-4 25 0.004725917064206309 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 740597.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.27479684314235 #Duplication Level Percentage of deduplicated Percentage of total 1 94.04636500446752 80.1978466959198 2 4.662238821927964 7.95142936748237 3 0.592381266280995 1.515455764073858 4 0.20948196642097913 0.7145412851540385 5 0.11469189232003059 0.4890163908573084 6 0.06788493020369495 0.347332417909859 7 0.05205130891327369 0.31070653550993405 8 0.0347257019403189 0.23689817425569665 9 0.028279549173445308 0.21703795295230827 >10 0.16032947964128086 2.6216655035457728 >50 0.017609900720639428 1.067262057210184 >100 0.011739240593610966 2.159586952642163 >500 9.518303126639023E-4 0.5677641201082048 >1k 0.0012691070835518699 1.603456782378497 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2109 0.28477025966888875 No Hit GTCCTACAGTGGACATTTCTAAATT 1765 0.23832124623783243 No Hit CTGTAGGACGTGGAATATGGCAAGA 1642 0.22171302341219312 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1379 0.1862011323297286 No Hit GGTATCAACGCAGAGTACTTTTTTT 1307 0.17647924579764704 No Hit GTCCTACAGTGTGCATTTCTCATTT 1289 0.17404877416462666 No Hit CTTTAGGACGTGAAATATGGCGAGG 1265 0.17080814532059946 No Hit TATCAACGCAGAGTACTTTTTTTTT 1097 0.14812374341240916 No Hit CTGTAGGACCTGGAATATGGCGAGA 938 0.1266545773207291 No Hit CACACACACACACACACACACACAC 759 0.10248488719235968 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 380 0.0 15.299038 1 TCTACAC 105 1.9324943E-8 12.691906 3 TTAATCA 60 4.021737E-4 12.691047 4 TGTAGGA 865 0.0 12.227466 2 CCAACGA 55 0.0030900987 12.078191 19 GTATTAT 95 9.978485E-7 12.038588 1 GTATAAG 135 3.5106495E-10 12.001431 1 TAGGACC 350 0.0 11.965844 4 CTGTAGG 840 0.0 11.7997265 1 TAGGACA 315 0.0 11.784544 4 GTCCTAA 455 0.0 11.729906 1 GTAGGAC 910 0.0 11.506398 3 GTGTAGG 100 1.8519404E-6 11.436659 1 GTCCATA 75 2.0101524E-4 11.436659 1 GGACATG 250 0.0 11.421169 6 GGACCTG 510 0.0 11.383845 6 GACGTGG 370 0.0 11.285414 7 GTCTTAA 120 1.2158125E-7 11.118974 1 GGTCTAC 120 1.2158125E-7 11.118974 1 TAACACT 130 3.162495E-8 10.982636 4 >>END_MODULE