FastQCFastQC Report
Thu 26 May 2016
SRR1926826_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926826_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657794
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT10850.16494525641766267No Hit
GTACATGGGAAGCAGTGGTATCAAC8390.1275475300778055No Hit
CCCATGTACTCTGCGTTGATACCAC7180.10915271346348553No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATC400.005155540514.2991943
TAGGACG1251.3551471E-912.1870724
TAGGACC1251.3551471E-912.1870724
GTTCTAA550.002961503812.1465771
GTAGGAC1900.012.0414263
GTGTAAG657.709577E-411.7461411
TGTTAAA657.7841803E-411.7326722
AGGACTA752.0337618E-411.4218915
GTATTAA1104.6652895E-711.2789641
GCCTTAT600.005672163311.1343621
CCTTATG600.005720836611.1215942
CCAATAC600.005720836611.1215943
TAGACAG600.0057862911.1046165
CTAAATT1553.2014214E-1011.01225619
GTATAGC1303.0378942E-811.0120081
GTTGTAA700.001433395810.907131
GTGTAAT700.001433395810.907131
TAAAACC1053.3647684E-610.8813144
CAATACT700.001462029410.8813144
TAGACTG700.0014657610.8779925
Produced by FastQC (version 0.11.5)