##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926826_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 657794 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.291779797322565 31.0 28.0 31.0 18.0 34.0 2 28.417579059705623 31.0 28.0 31.0 18.0 34.0 3 28.200158408255472 31.0 28.0 33.0 16.0 34.0 4 31.880432171774142 35.0 32.0 37.0 19.0 37.0 5 31.621066777745007 35.0 32.0 37.0 19.0 37.0 6 31.368870801497124 35.0 32.0 37.0 17.0 37.0 7 31.106373727945222 35.0 32.0 37.0 16.0 37.0 8 30.920196900549413 35.0 32.0 37.0 11.0 37.0 9 33.17376108629753 37.0 33.0 39.0 17.0 39.0 10 33.401169667099424 37.0 34.0 39.0 17.0 39.0 11 33.546456793464216 38.0 34.0 39.0 19.0 39.0 12 33.44202744324211 38.0 34.0 39.0 18.0 39.0 13 33.54124543550108 38.0 34.0 39.0 19.0 39.0 14 34.58328139204675 39.0 34.0 41.0 19.0 41.0 15 34.12488560248345 39.0 33.0 41.0 16.0 41.0 16 34.236254815337325 39.0 34.0 41.0 17.0 41.0 17 34.45950099879293 39.0 34.0 41.0 18.0 41.0 18 34.517075254562975 39.0 34.0 41.0 18.0 41.0 19 33.84736558861893 39.0 33.0 41.0 10.0 41.0 20 34.13182546511522 39.0 34.0 41.0 16.0 41.0 21 34.11258387884353 39.0 34.0 41.0 11.0 41.0 22 34.148227256557526 39.0 34.0 41.0 10.0 41.0 23 34.13959841530935 39.0 34.0 41.0 2.0 41.0 24 32.792705619084394 39.0 32.0 40.0 2.0 41.0 25 33.393816301152036 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25459348950859706 1101 2 0.04921555171951297 1101 3 -0.7947023510993994 1101 4 -0.03155678196903722 1101 5 -0.01605336348632136 1101 6 -1.760147706255065 1101 7 -0.3853783518727347 1101 8 0.230409046926507 1101 9 0.24325300638515301 1101 10 -8.561073032675345 1101 11 -3.250972217896816 1101 12 -0.9621606525115425 1101 13 -0.1644240735153204 1101 14 -0.098879924698835 1101 15 -1.2676927854402962 1101 16 0.10254728359409881 1101 17 0.28190611450707337 1101 18 0.34872798771547764 1101 19 -0.38947989591229515 1101 20 0.36816166819427565 1101 21 0.3007893981859766 1101 22 0.3493651260909303 1101 23 0.4146227660084634 1101 24 0.5634991490701893 1101 25 0.6374713504461837 1102 1 0.46087190251314425 1102 2 0.5647135541991943 1102 3 0.22295676172605638 1102 4 0.22556368917024017 1102 5 0.3949881022803119 1102 6 -0.5988221104429705 1102 7 0.2608539115244284 1102 8 0.6079834373384116 1102 9 0.43718467741421563 1102 10 0.6352780487408296 1102 11 0.47496282825279224 1102 12 0.3512778376386194 1102 13 0.014715615147025574 1102 14 -0.0462814262830733 1102 15 0.5996270933308807 1102 16 0.5398320335596622 1102 17 0.08412577286222245 1102 18 -0.01481210045093917 1102 19 0.42716151475033115 1102 20 0.3555910661829742 1102 21 0.009538881585896775 1102 22 0.16373349638986667 1102 23 -0.15466191507709226 1102 24 -3.7702589443311076 1102 25 -2.016346324164644 1103 1 0.07656204360025143 1103 2 -0.2871643135123101 1103 3 0.19321418985068206 1103 4 0.151744284107604 1103 5 -0.10418487979595525 1103 6 0.6833950709081016 1103 7 0.4792081243752122 1103 8 0.4498662027144995 1103 9 0.3609572539294845 1103 10 0.4197614740127733 1103 11 0.3158230987413546 1103 12 0.2586647187714419 1103 13 0.05459936005603083 1103 14 0.36634896329606903 1103 15 0.5719724732006242 1103 16 0.708094737414207 1103 17 0.5901275600801767 1103 18 0.4409306260875354 1103 19 -1.1533212918692541 1103 20 -0.14930874650283243 1103 21 0.5370087652448774 1103 22 0.4535506502452762 1103 23 0.7150623549990414 1103 24 2.151752191411532 1103 25 1.4421266355713982 1104 1 -0.2690066917085794 1104 2 -0.27881899699938373 1104 3 -1.2168198654038136 1104 4 -0.7837223691630228 1104 5 -0.8365204171970788 1104 6 -1.7638402159451516 1104 7 -0.5099609650915227 1104 8 -0.2447040040659907 1104 9 -0.01410894136390084 1104 10 -0.13514869923574224 1104 11 0.0465333192946531 1104 12 -0.01079565091750112 1104 13 -0.14231306121605058 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-1.9452142966515282 2215 1 0.43156348862880733 2215 2 -0.04876752696314668 2215 3 0.23208545792725488 2215 4 0.35132284793213486 2215 5 0.9099965206745004 2215 6 1.107225603812676 2215 7 0.7873906442855088 2215 8 -0.35844069940036505 2215 9 0.029610377569056823 2215 10 -0.26484183821216334 2215 11 -0.07196220294036948 2215 12 -0.4892875477284093 2215 13 -0.17019403744388484 2215 14 0.05725918495247839 2215 15 0.18550598871730273 2215 16 0.21883001915819733 2215 17 -0.23483741169604144 2215 18 -1.03555930809965 2215 19 0.1868896457164908 2215 20 -4.012080421016556 2215 21 -2.2970635447155736 2215 22 -0.9454162457968138 2215 23 -1.0568225109791385 2215 24 -0.9605484769599002 2215 25 -0.7224090712670588 2216 1 -0.8930613374495096 2216 2 -1.533420491085586 2216 3 -0.792055493175905 2216 4 -1.0414326927158477 2216 5 -0.9639273388961591 2216 6 -2.372938006801512 2216 7 -10.188528966626833 2216 8 -11.56528482830118 2216 9 -6.407703277767855 2216 10 -3.901749262183543 2216 11 -2.9587221040534644 2216 12 -2.4872346506900485 2216 13 -2.3258539460107706 2216 14 -2.3042106396350803 2216 15 -3.490825754474905 2216 16 -2.6875255761508896 2216 17 -2.9500639446914647 2216 18 -2.6258137927945384 2216 19 -2.049415574418674 2216 20 -2.80073917424653 2216 21 -5.413634554455186 2216 22 -4.0485316179892195 2216 23 -3.7945501620425404 2216 24 -4.04812425712289 2216 25 -3.623941445546528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36480.0 3 2.0 4 268.0 5 1790.0 6 2330.0 7 1809.0 8 1491.0 9 1298.0 10 1193.0 11 1149.0 12 1172.0 13 1193.0 14 1186.0 15 1224.0 16 1355.0 17 1376.0 18 1628.0 19 2066.0 20 2667.0 21 3425.0 22 4259.0 23 5112.0 24 5958.0 25 6843.0 26 7728.0 27 8581.0 28 9609.0 29 11291.0 30 13590.0 31 16652.0 32 21145.0 33 28060.0 34 38016.0 35 52879.0 36 76852.0 37 122229.0 38 163888.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.75330746889105 17.528442251486496 11.769591095967646 25.94865918365481 2 15.695794358374746 19.738228344538634 39.06189101627204 25.504086280814576 3 18.861705188891108 24.054707090515173 29.41533814347289 27.66824957712083 4 12.52162113234643 16.426431862817434 36.4214725037005 34.63047450113563 5 13.435504741862351 38.21361721150819 34.473498942451414 13.87737910417804 6 33.56040032721976 35.71926004543367 17.40529329655659 13.315046330789981 7 28.615669872327576 31.861077522914094 21.609620947243066 17.913631657515257 8 26.433887832482196 34.961656757453255 19.980725448964375 18.623729961100175 9 26.89457927043436 15.319327513878351 18.98129634669801 38.80479686898928 10 15.333412413088308 28.419286943932455 33.42640412188015 22.820896521099087 11 35.0773375743689 22.65056613871653 22.959521119779353 19.31257516713522 12 23.5337316973042 24.715139351366144 30.238562240569266 21.51256671076039 13 28.930883591657015 21.542431070606444 25.081571897303036 24.44511344043351 14 22.10842411756209 20.97020655471001 26.455119774363496 30.4662495533644 15 24.184435551555104 29.06018512887256 22.728309391458772 24.02706992811356 16 23.549097809411002 27.608081170544203 25.549319802881964 23.29350121716283 17 22.87064039678509 27.632487235550094 26.332322719352398 23.164549648312416 18 23.046128443212556 27.260010916715196 27.066003554774394 22.62785708529786 19 24.388923608418448 26.70215661498272 26.325513076084427 22.58340670051441 20 24.327689886649587 26.554710009959027 26.398254510069258 22.71934559332213 21 23.99855861773265 26.239695181100252 26.240911537444013 23.520834663723083 22 24.21349843619572 26.322695612377238 26.114087010465404 23.349718940961637 23 23.63940458270614 26.394958110964133 26.355273761194482 23.610363545135247 24 23.925838415445362 26.64273474644176 26.177476322755545 23.25395051535733 25 23.771772977180948 26.130157397946775 26.380577044713664 23.717492580158613 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 423.0 1 423.0 2 475.5 3 528.0 4 528.0 5 528.0 6 660.5 7 793.0 8 793.0 9 793.0 10 1052.5 11 1312.0 12 1312.0 13 1312.0 14 1993.5 15 2675.0 16 2675.0 17 2675.0 18 4529.0 19 6383.0 20 6383.0 21 6383.0 22 9561.0 23 12739.0 24 12739.0 25 12739.0 26 18023.5 27 23308.0 28 23308.0 29 23308.0 30 30116.0 31 36924.0 32 36924.0 33 36924.0 34 44049.5 35 51175.0 36 51175.0 37 51175.0 38 57662.5 39 64150.0 40 64150.0 41 64150.0 42 70187.5 43 76225.0 44 76225.0 45 76225.0 46 81706.5 47 87188.0 48 87188.0 49 87188.0 50 86485.5 51 85783.0 52 85783.0 53 85783.0 54 80167.0 55 74551.0 56 74551.0 57 74551.0 58 65558.5 59 56566.0 60 56566.0 61 56566.0 62 47192.5 63 37819.0 64 37819.0 65 37819.0 66 29867.5 67 21916.0 68 21916.0 69 21916.0 70 16211.0 71 10506.0 72 10506.0 73 10506.0 74 7429.0 75 4352.0 76 4352.0 77 4352.0 78 3025.5 79 1699.0 80 1699.0 81 1699.0 82 1139.5 83 580.0 84 580.0 85 580.0 86 363.5 87 147.0 88 147.0 89 147.0 90 95.0 91 43.0 92 43.0 93 43.0 94 24.0 95 5.0 96 5.0 97 5.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23594012715226956 2 0.1462463932477341 3 0.15004697519284152 4 0.06856249828973812 5 0.021435282170405932 6 0.020067072670167257 7 0.3002459736634874 8 0.30511071855322486 9 0.0174826769474942 10 0.0349653538949884 11 0.014138164835799657 12 0.01581042089164693 13 0.01398614155799536 14 0.015050304502625442 15 0.013682095002386766 16 0.017786723503102796 17 0.016418514002864117 18 0.013226025168973872 19 0.02173932872601453 20 0.015050304502625442 21 0.01398614155799536 22 0.01565839761384263 23 0.01581042089164693 24 0.014442211391408251 25 0.014898281224821145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 657794.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.44622773103526 #Duplication Level Percentage of deduplicated Percentage of total 1 90.77035018212518 76.65213662705561 2 6.655442833431516 11.240540823256888 3 1.3666555745772924 3.4622672364192866 4 0.48017575631042425 1.621961250732487 5 0.24010203045969478 1.0137855371441675 6 0.13614474318377937 0.6898145992368445 7 0.0842677157732746 0.4981263501594869 8 0.05522882145224634 0.37310925069384643 9 0.03529963083837189 0.2682828598138765 >10 0.16209738472190371 2.2270845510853903 >50 0.0073880654469295395 0.41465913591652087 >100 0.0059463061960460875 0.9645712974609418 >500 7.207643865907217E-4 0.4085619075732129 >1k 1.8019109664768043E-4 0.16509857345137283 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1085 0.16494525641766267 No Hit GTACATGGGAAGCAGTGGTATCAAC 839 0.1275475300778055 No Hit CCCATGTACTCTGCGTTGATACCAC 718 0.10915271346348553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.520232778042974E-4 7 0.0 0.0 0.0 0.0 1.520232778042974E-4 8 0.0 0.0 0.0 0.0 1.520232778042974E-4 9 0.0 0.0 0.0 0.0 1.520232778042974E-4 10 0.0 0.0 0.0 0.0 1.520232778042974E-4 11 0.0 0.0 0.0 0.0 1.520232778042974E-4 12 0.0 0.0 0.0 0.0 1.520232778042974E-4 13 1.520232778042974E-4 0.0 0.0 0.0 1.520232778042974E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAATC 40 0.0051555405 14.299194 3 TAGGACG 125 1.3551471E-9 12.187072 4 TAGGACC 125 1.3551471E-9 12.187072 4 GTTCTAA 55 0.0029615038 12.146577 1 GTAGGAC 190 0.0 12.041426 3 GTGTAAG 65 7.709577E-4 11.746141 1 TGTTAAA 65 7.7841803E-4 11.732672 2 AGGACTA 75 2.0337618E-4 11.421891 5 GTATTAA 110 4.6652895E-7 11.278964 1 GCCTTAT 60 0.0056721633 11.134362 1 CCTTATG 60 0.0057208366 11.121594 2 CCAATAC 60 0.0057208366 11.121594 3 TAGACAG 60 0.00578629 11.104616 5 CTAAATT 155 3.2014214E-10 11.012256 19 GTATAGC 130 3.0378942E-8 11.012008 1 GTTGTAA 70 0.0014333958 10.90713 1 GTGTAAT 70 0.0014333958 10.90713 1 TAAAACC 105 3.3647684E-6 10.881314 4 CAATACT 70 0.0014620294 10.881314 4 TAGACTG 70 0.00146576 10.877992 5 >>END_MODULE