FastQCFastQC Report
Thu 26 May 2016
SRR1926826_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926826_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences657794
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20770.3157523479995257No Hit
GGTATCAACGCAGAGTACTTTTTTT13790.20964010009212614No Hit
TATCAACGCAGAGTACTTTTTTTTT11770.17893139797565805No Hit
GTACATGGGAAGCAGTGGTATCAAC10070.15308744074892747No Hit
CCCATGTACTCTGCGTTGATACCAC8640.13134811202291294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC807.4469426E-915.4745133
CTATACT1051.3169483E-913.602934
GTCCTAA1204.9112714E-1113.5081241
CTAAAGT2000.012.3786664
GTAGGAC2600.012.0866023
GTCCTAC2100.011.805421
TAGGACG2350.011.7506174
TGTAGGA2600.011.7337712
AAAGTGT2050.011.6122576
GTTCTAG752.0005085E-411.4421771
CCATAAC600.005710349411.1243381
TTCTATA1201.2093733E-711.1226392
CTTAGAT600.00571685111.1226392
TCTAATC600.005765813411.1099063
TCTATCC600.005765813411.1099063
GCACCGT600.005769090311.1090596
GTCTTAG700.001444215810.897311
CTAGACA700.001460877210.8823444
TAAAGTG1951.8189894E-1210.7428275
TTAGGAC1909.094947E-1210.5251753
Produced by FastQC (version 0.11.5)