FastQCFastQC Report
Thu 26 May 2016
SRR1926825_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926825_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706067
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT19140.2710790902279812No Hit
CTGTAGGACGTGGAATATGGCAAGA14250.2018222066744374No Hit
GTCCTACAGTGTGCATTTCTCATTT13760.19488235535721116No Hit
GTCCTAAAGTGTGTATTTCTCATTT13240.18751761518382817No Hit
GTATCAACGCAGAGTACTTTTTTTT11440.16202428381442555No Hit
CTTTAGGACGTGAAATATGGCGAGG10730.1519685808853834No Hit
CTGTAGGACCTGGAATATGGCGAGA9200.1302992492213912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3700.015.7001614
TAATCTC508.550991E-515.2314615
AGGACGA400.005203430514.2794945
TTAGTAC706.9871057E-613.6198583
GTCTAGG852.5442932E-713.4740191
TGTAGGA8700.013.370332
CTGTAGG8550.013.2835971
GTCCTAG1259.458745E-1112.9799731
GTAGGAC9100.012.5721773
TAATACT1001.3933641E-712.3799644
GGACCTG6250.012.1851696
GTCCTAC9200.012.1376021
CCAACGA550.003111333412.06702119
ACAGGTC951.0228523E-612.0137318
TCCAACG951.0621588E-611.97629218
TAGGACG8150.011.9184174
TTAGGAC4750.011.8421093
AGGACGT7800.011.8385555
AGGACCT6850.011.8127215
GGCAAGA6050.011.753592519
Produced by FastQC (version 0.11.5)