##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926825_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 706067 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.67492320133925 31.0 30.0 33.0 23.0 34.0 2 28.83380047502574 31.0 30.0 33.0 23.0 34.0 3 28.609844391537912 31.0 30.0 33.0 16.0 34.0 4 32.25377336711672 35.0 33.0 37.0 23.0 37.0 5 32.06297560996336 35.0 32.0 37.0 19.0 37.0 6 31.812091487068507 35.0 32.0 37.0 17.0 37.0 7 31.61462722376205 35.0 32.0 37.0 17.0 37.0 8 31.44034206385513 35.0 32.0 37.0 17.0 37.0 9 33.64582114728489 37.0 34.0 39.0 21.0 39.0 10 33.8303008071472 38.0 34.0 39.0 23.0 39.0 11 33.98634973734787 38.0 35.0 39.0 25.0 39.0 12 33.88720900424464 38.0 35.0 39.0 23.0 39.0 13 33.99194693987965 38.0 35.0 39.0 25.0 39.0 14 35.05278252630416 39.0 36.0 41.0 25.0 41.0 15 34.670451954276295 39.0 34.0 41.0 19.0 41.0 16 34.7426207427907 39.0 34.0 41.0 21.0 41.0 17 34.97074356966124 39.0 35.0 41.0 24.0 41.0 18 35.018595968937795 39.0 36.0 41.0 24.0 41.0 19 34.41934972176861 39.0 34.0 41.0 18.0 41.0 20 34.72482214860629 39.0 34.0 41.0 18.0 41.0 21 34.698202861768074 39.0 35.0 41.0 18.0 41.0 22 34.735401881124595 39.0 35.0 41.0 18.0 41.0 23 34.75349789750831 39.0 35.0 41.0 18.0 41.0 24 33.496695072847196 39.0 33.0 41.0 2.0 41.0 25 34.07972331237687 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.002226005911776241 1101 2 -0.07726099242660567 1101 3 -0.9062747355078429 1101 4 -0.1840220387506477 1101 5 -0.15876198303919864 1101 6 -2.1276341168676467 1101 7 -0.2459047002631003 1101 8 0.25826793190864805 1101 9 -0.010616093420701134 1101 10 -9.344810854484592 1101 11 -3.861204923380196 1101 12 -1.260848965090105 1101 13 -0.4600168819507786 1101 14 -0.12522098593438358 1101 15 -1.6245831964477162 1101 16 -0.09766183113310234 1101 17 0.09324891255778311 1101 18 0.11648597585699605 1101 19 -0.4750110642635832 1101 20 0.09331807618158905 1101 21 0.06348890527107187 1101 22 0.22496622870070127 1101 23 0.14720710855097252 1101 24 0.013648279463055246 1101 25 0.0907080783869958 1102 1 -0.14974674112736963 1102 2 -0.19305116627168672 1102 3 -0.44330527156559185 1102 4 -0.15194042449863332 1102 5 -0.12035956927348934 1102 6 -0.929663387287377 1102 7 -0.01981278722813684 1102 8 0.3607652112488182 1102 9 0.359203336062933 1102 10 0.2299162942823827 1102 11 0.18318386583841573 1102 12 0.15348674123753625 1102 13 -0.01766530803289612 1102 14 -0.4611979938182955 1102 15 0.03319474967120328 1102 16 -0.040303485589902266 1102 17 -0.3178321640290207 1102 18 -0.4184576432025864 1102 19 0.027450001200385543 1102 20 -0.17534638932192337 1102 21 -0.16412824015166194 1102 22 -0.08437233224115204 1102 23 -0.6643393902508308 1102 24 -4.175212627842356 1102 25 -2.2266492694269644 1103 1 -0.1675394894028841 1103 2 -0.296304486585786 1103 3 -0.06554593365184402 1103 4 -0.10780741336701283 1103 5 -0.34961494995395626 1103 6 0.16967596561913112 1103 7 0.01592026871782437 1103 8 0.26093672627391484 1103 9 0.11348506338227793 1103 10 0.20542052386294074 1103 11 0.02893784424919943 1103 12 -0.23014673579921663 1103 13 -0.03848848906732627 1103 14 -0.09744404637676496 1103 15 0.3059136590433198 1103 16 0.2939258308184307 1103 17 0.025470233446803547 1103 18 -0.04576997634433155 1103 19 -1.7592993473066656 1103 20 -0.6842942270758172 1103 21 -0.4699469078724974 1103 22 -0.35413641027275133 1103 23 -0.24669256182141908 1103 24 1.1299356465780406 1103 25 0.5072583310659908 1104 1 -0.12926435093742938 1104 2 0.05122658695412596 1104 3 -1.3517520455732566 1104 4 -0.33562220922799924 1104 5 -0.17599805703768823 1104 6 -1.5692827602109851 1104 7 -0.34979243279049754 1104 8 0.3898122551365404 1104 9 0.2402455776464265 1104 10 0.3349499113194909 1104 11 0.19394821444650034 1104 12 0.11787691121478616 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-1.0256543742739197 2215 1 0.5693408720405024 2215 2 0.15167194794240402 2215 3 0.5875368838550692 2215 4 0.7638523203272385 2215 5 1.0360952982398217 2215 6 1.3948488255200147 2215 7 1.0875306423022053 2215 8 -0.10756435845430445 2215 9 0.19422705729188294 2215 10 0.03827445376361993 2215 11 0.08126752201525278 2215 12 -0.06939916897398746 2215 13 0.22289239981271436 2215 14 0.20640564412738627 2215 15 0.1759182367954466 2215 16 0.4174288063573073 2215 17 0.10714370039742249 2215 18 -0.6092243879041561 2215 19 0.22187375143345633 2215 20 -2.9457953317073056 2215 21 -1.5166750092995613 2215 22 -0.6213513456866409 2215 23 -0.5238583361581419 2215 24 -0.31567627946140675 2215 25 -0.14654816934202586 2216 1 -0.964157646677517 2216 2 -1.7021066677322523 2216 3 -0.8595337688067488 2216 4 -1.1912783018111988 2216 5 -0.8100057880336315 2216 6 -2.2279431665798164 2216 7 -10.833769294855529 2216 8 -11.806388310335084 2216 9 -6.460855086527133 2216 10 -4.100430995558579 2216 11 -3.0937392110846638 2216 12 -2.4694278968669785 2216 13 -2.335549111992652 2216 14 -2.4637945533768786 2216 15 -3.409064257144763 2216 16 -2.607392093184842 2216 17 -2.770866893013121 2216 18 -2.6199506649487745 2216 19 -1.8509011834799125 2216 20 -2.696670634696801 2216 21 -5.365361605941988 2216 22 -4.118322348469654 2216 23 -4.067325433743203 2216 24 -4.35463489334057 2216 25 -3.7539456017865866 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34756.0 3 1.0 4 282.0 5 1668.0 6 2272.0 7 1798.0 8 1374.0 9 1265.0 10 1122.0 11 1156.0 12 1096.0 13 1116.0 14 1108.0 15 1166.0 16 1272.0 17 1338.0 18 1578.0 19 1885.0 20 2653.0 21 3249.0 22 4139.0 23 4998.0 24 5866.0 25 6648.0 26 7674.0 27 8451.0 28 9299.0 29 11318.0 30 13303.0 31 16468.0 32 21326.0 33 28738.0 34 39073.0 35 55101.0 36 81474.0 37 132131.0 38 197905.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69242453925719 19.18041583972879 12.766927027878808 25.36023259313521 2 13.385374487826256 20.356752325612728 43.65698790071523 22.600885285845777 3 18.881176407181215 24.848311212347284 29.96062757601459 26.309884804456914 4 11.019096725182335 17.467820161402337 39.82561517986978 31.68746793354555 5 11.917484530474399 39.27517056143612 36.52040504091208 12.286939867177407 6 30.726046472753954 38.54238234131678 18.888711027752475 11.842860158176787 7 26.722801773554938 32.27517662385264 22.93332689299713 18.068694709595295 8 23.36087637732976 39.153168586440984 21.16794438549466 16.3180106507346 9 25.687281937520982 15.988486159451393 20.762593617241297 37.56163828578633 10 13.449130810535115 30.730062479633908 35.8322825609566 19.988524148874376 11 33.629008040161146 23.244224852540384 25.19802933893897 17.9287377683595 12 22.396330597524454 25.540905395030215 33.28484048354973 18.777923523895595 13 28.525290528021575 23.02438112290284 26.128965544594845 22.32136280448073 14 19.250028684872944 23.700079467722084 28.611496330461065 28.438395516943903 15 21.91883664632082 33.14016819673664 23.539185703419037 21.401809453523505 16 20.266627380373947 29.602835412626483 29.79322283119124 20.337314375808333 17 20.152335245196852 29.888957040440943 29.29344153848115 20.665266175881055 18 19.86497933328234 29.062306116725168 31.57418345926008 19.498531090732417 19 22.666228452916577 27.747288552421317 28.117031404059524 21.46945159060258 20 22.096401550833423 28.914374106691334 29.503373501826637 19.48585084064861 21 21.687156583191328 28.036126846252674 27.86685308028676 22.409863490269238 22 21.169727824665483 30.039861631692123 28.653062149581693 20.1373483940607 23 20.93200819335409 29.244636214511754 28.800262912607057 21.023092679527096 24 22.066358149292377 28.119276386522124 29.655488932045436 20.15887653214006 25 21.39070990260016 28.57132739150882 29.405373706010007 20.632588999881012 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 247.0 1 247.0 2 299.5 3 352.0 4 352.0 5 352.0 6 583.5 7 815.0 8 815.0 9 815.0 10 1284.5 11 1754.0 12 1754.0 13 1754.0 14 3024.0 15 4294.0 16 4294.0 17 4294.0 18 7181.0 19 10068.0 20 10068.0 21 10068.0 22 15849.0 23 21630.0 24 21630.0 25 21630.0 26 30847.0 27 40064.0 28 40064.0 29 40064.0 30 52508.0 31 64952.0 32 64952.0 33 64952.0 34 72339.0 35 79726.0 36 79726.0 37 79726.0 38 84367.5 39 89009.0 40 89009.0 41 89009.0 42 90942.0 43 92875.0 44 92875.0 45 92875.0 46 92637.5 47 92400.0 48 92400.0 49 92400.0 50 86039.5 51 79679.0 52 79679.0 53 79679.0 54 67568.0 55 55457.0 56 55457.0 57 55457.0 58 45650.0 59 35843.0 60 35843.0 61 35843.0 62 28099.0 63 20355.0 64 20355.0 65 20355.0 66 15230.0 67 10105.0 68 10105.0 69 10105.0 70 7156.5 71 4208.0 72 4208.0 73 4208.0 74 2845.5 75 1483.0 76 1483.0 77 1483.0 78 1008.5 79 534.0 80 534.0 81 534.0 82 345.0 83 156.0 84 156.0 85 156.0 86 99.0 87 42.0 88 42.0 89 42.0 90 26.0 91 10.0 92 10.0 93 10.0 94 7.5 95 5.0 96 5.0 97 5.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23524679669209864 2 0.139363544819401 3 0.14233776681249796 4 0.07095643897817062 5 0.023085627851181262 6 0.02096118357039771 7 0.30733627262002045 8 0.2943063476978814 9 0.01897836890833306 10 0.03356621963638012 11 0.015720887677798282 12 0.017137183864987318 13 0.015720887677798282 14 0.01699555424626841 15 0.015295998821641572 16 0.019544887383208673 17 0.017562072721144028 18 0.01430459149060925 19 0.024218664800932488 20 0.017562072721144028 21 0.015579258059079379 22 0.01770370233986293 23 0.018270220814738544 24 0.015579258059079379 25 0.015720887677798282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 706067.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.55028686705467 #Duplication Level Percentage of deduplicated Percentage of total 1 95.22608247223188 82.41844755197475 2 3.5963014773744466 6.225218490543417 3 0.5207135506455383 1.3520372155180183 4 0.1893364821211247 0.6554850736792924 5 0.10905219510095891 0.4719249384735004 6 0.07253581663463228 0.37667974427180995 7 0.046933412109177404 0.2843470197189301 8 0.03480902110227434 0.24101846095705629 9 0.021652725451654392 0.16866446393648374 >10 0.14983835132250936 2.476143082881518 >50 0.01751869804443979 1.027355343017855 >100 0.01293374227187951 2.4284309608271606 >500 0.0013097460511637096 0.7043838428237131 >1k 9.823095383727821E-4 1.1698638113764988 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1914 0.2710790902279812 No Hit CTGTAGGACGTGGAATATGGCAAGA 1425 0.2018222066744374 No Hit GTCCTACAGTGTGCATTTCTCATTT 1376 0.19488235535721116 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1324 0.18751761518382817 No Hit GTATCAACGCAGAGTACTTTTTTTT 1144 0.16202428381442555 No Hit CTTTAGGACGTGAAATATGGCGAGG 1073 0.1519685808853834 No Hit CTGTAGGACCTGGAATATGGCGAGA 920 0.1302992492213912 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.832592374378069E-4 0.0 10 0.0 0.0 0.0 4.2488885615671036E-4 0.0 11 0.0 0.0 0.0 4.2488885615671036E-4 0.0 12 0.0 0.0 0.0 5.665184748756138E-4 0.0 13 0.0 0.0 0.0 7.081480935945173E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 370 0.0 15.700161 4 TAATCTC 50 8.550991E-5 15.231461 5 AGGACGA 40 0.0052034305 14.279494 5 TTAGTAC 70 6.9871057E-6 13.619858 3 GTCTAGG 85 2.5442932E-7 13.474019 1 TGTAGGA 870 0.0 13.37033 2 CTGTAGG 855 0.0 13.283597 1 GTCCTAG 125 9.458745E-11 12.979973 1 GTAGGAC 910 0.0 12.572177 3 TAATACT 100 1.3933641E-7 12.379964 4 GGACCTG 625 0.0 12.185169 6 GTCCTAC 920 0.0 12.137602 1 CCAACGA 55 0.0031113334 12.067021 19 ACAGGTC 95 1.0228523E-6 12.013731 8 TCCAACG 95 1.0621588E-6 11.976292 18 TAGGACG 815 0.0 11.918417 4 TTAGGAC 475 0.0 11.842109 3 AGGACGT 780 0.0 11.838555 5 AGGACCT 685 0.0 11.812721 5 GGCAAGA 605 0.0 11.7535925 19 >>END_MODULE