FastQCFastQC Report
Thu 26 May 2016
SRR1926825_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926825_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706067
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19550.27688590459545626No Hit
GTCCTACAGTGGACATTTCTAAATT18700.26484738700434945No Hit
CTGTAGGACGTGGAATATGGCAAGA17310.24516087000242187No Hit
GTCCTACAGTGTGCATTTCTCATTT13840.19601539230696238No Hit
GTCCTAAAGTGTGTATTTCTCATTT13630.1930411703138654No Hit
CTTTAGGACGTGAAATATGGCGAGG12800.18128591196019642No Hit
GGTATCAACGCAGAGTACTTTTTTT11770.16669806123214936No Hit
CTGTAGGACCTGGAATATGGCGAGA11060.15664235830310722No Hit
TATCAACGCAGAGTACTTTTTTTTT10160.1438956926184059No Hit
CTGAAGGACCTGGAATATGGCGAGA7560.10707199175149101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGTG250.00605962218.983287
TTCCCGT602.5926387E-514.23342612
TAGGACC4450.014.1164934
GATATAC3750.013.4730771
GTGCTAG500.001459667513.3459721
GACGTGG4200.013.3334957
GTAATCT655.3487136E-513.1786844
TGTAGGA10150.013.0520352
GTCCTAT957.039307E-813.0449351
GTAGGAC9850.013.044893
CTGTAGG10150.012.8669831
GGACCTG6400.012.7897296
TTATAGT604.022559E-412.6905844
AGGACCT6750.012.5495785
TCCAACG1155.3951226E-912.37689218
GTAGACT550.00302257112.113744
GTTCTAG1209.475116E-911.9160471
TTATACT802.806437E-511.8974234
TCAGCAC1052.6459566E-711.7841153
AGGACGT7800.011.7143855