##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926825_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 706067 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.842298252148876 31.0 30.0 33.0 26.0 34.0 2 30.18272061999782 31.0 30.0 34.0 26.0 34.0 3 30.25235707092953 31.0 30.0 34.0 26.0 34.0 4 33.82998497309745 35.0 35.0 37.0 29.0 37.0 5 33.803688601789915 37.0 35.0 37.0 30.0 37.0 6 33.72949734232021 37.0 35.0 37.0 29.0 37.0 7 33.744929305575816 37.0 35.0 37.0 29.0 37.0 8 33.77754802306297 37.0 35.0 37.0 29.0 37.0 9 35.33740140808167 39.0 35.0 39.0 30.0 39.0 10 35.22566696928195 39.0 35.0 39.0 29.0 39.0 11 35.26992480883542 39.0 35.0 39.0 30.0 39.0 12 35.16982524321346 39.0 35.0 39.0 29.0 39.0 13 35.17914588842135 39.0 35.0 39.0 29.0 39.0 14 36.27371481743234 40.0 36.0 41.0 29.0 41.0 15 36.267657318639735 40.0 36.0 41.0 29.0 41.0 16 36.253985811544794 40.0 36.0 41.0 29.0 41.0 17 36.23377526495361 40.0 36.0 41.0 29.0 41.0 18 36.21708138179521 40.0 36.0 41.0 29.0 41.0 19 36.2897161317552 40.0 37.0 41.0 29.0 41.0 20 36.255821331403396 40.0 37.0 41.0 29.0 41.0 21 36.20272721993805 40.0 37.0 41.0 29.0 41.0 22 36.114169051945495 40.0 37.0 41.0 29.0 41.0 23 36.044682728409626 40.0 37.0 41.0 29.0 41.0 24 35.98188273917348 40.0 37.0 41.0 28.0 41.0 25 35.87045138775782 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.392476133374835 1101 2 -0.1424981912044636 1101 3 0.05245727465636563 1101 4 -0.1164315564024676 1101 5 -0.0863309284869942 1101 6 -0.09655017107303365 1101 7 -0.11474489447480352 1101 8 -0.16141239020980436 1101 9 -0.09695693458349552 1101 10 -0.06525164205412182 1101 11 -0.07364857490193089 1101 12 -0.12032146638741636 1101 13 0.12667906708832533 1101 14 -0.03499946008482624 1101 15 0.019653815515688677 1101 16 0.023789867843717616 1101 17 -0.10230163348234811 1101 18 -0.11196911517141217 1101 19 -0.22592450584826906 1101 20 -0.09593878941142009 1101 21 0.02813510553404086 1101 22 -0.22621367858560149 1101 23 -0.10531377161126443 1101 24 -0.030690718876762446 1101 25 0.0010585165260010854 1102 1 -0.5540731653151028 1102 2 -0.56628830592485 1102 3 -0.30984592786973764 1102 4 -0.38304698166608375 1102 5 -0.33494473151676374 1102 6 -0.37696936516648094 1102 7 -0.2866582620142424 1102 8 -0.255628516840126 1102 9 -0.20507013620424885 1102 10 -0.2363298732649426 1102 11 -0.09115595163156343 1102 12 -0.26672899443931186 1102 13 -0.2182470178036553 1102 14 -0.25858411648719937 1102 15 -0.3849928312826165 1102 16 -0.3236074445693262 1102 17 -0.30221516361469014 1102 18 -0.15481511935817593 1102 19 -0.34516885528260843 1102 20 -0.1697458100995206 1102 21 -0.26620610678089207 1102 22 -0.27527147112812855 1102 23 -0.2962098618059912 1102 24 -0.2933420180495645 1102 25 -0.38045836449848736 1103 1 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0.8222498646472829 2208 15 0.7817534160075539 2208 16 0.695404440759603 2208 17 0.6510851415286538 2208 18 0.49379879442878405 2208 19 0.599487655409007 2208 20 0.6207191519650621 2208 21 0.7098973938442938 2208 22 0.6640381141571581 2208 23 0.6916058241150225 2208 24 0.6292708086809569 2208 25 0.5738004780987751 2209 1 0.3245599298942956 2209 2 0.35303424174401243 2209 3 0.4079498982705978 2209 4 0.3175966379333204 2209 5 0.3043395614077582 2209 6 0.19421680069714142 2209 7 0.13050747068290036 2209 8 0.07005878097201901 2209 9 0.1587808645145401 2209 10 0.16399926320270453 2209 11 0.11478931425711636 2209 12 0.08609905603405821 2209 13 0.21894176138270893 2209 14 0.14193664052667998 2209 15 0.20083106336596757 2209 16 0.1048101425551593 2209 17 0.024655672865350198 2209 18 0.00587648341329583 2209 19 -0.03133948774313211 2209 20 0.041826683594770486 2209 21 0.036904649027768244 2209 22 -0.1157165857146154 2209 23 -0.012986023863213347 2209 24 0.04401852597936795 2209 25 0.03151367104062075 2210 1 -0.035366133949843004 2210 2 -0.11972779272248957 2210 3 0.37112785139145643 2210 4 0.21437173265508846 2210 5 0.11193254112372841 2210 6 0.013219249891832874 2210 7 0.0644569915582025 2210 8 0.14570048426549675 2210 9 0.18062917317339355 2210 10 0.254396451787521 2210 11 0.2791488490529872 2210 12 0.253003816805041 2210 13 0.08651461421039386 2210 14 0.046939473846521196 2210 15 -0.15969182303298624 2210 16 0.1256529004467879 2210 17 0.10866955851351179 2210 18 0.18433357012558105 2210 19 -0.05069018924773161 2210 20 0.09648832806313123 2210 21 0.33570798805948243 2210 22 0.35934781874060207 2210 23 0.2695927892535508 2210 24 0.4442388423905612 2210 25 0.3424994917430624 2211 1 -0.19044634785358738 2211 2 -0.29085078737489667 2211 3 0.02175173730946156 2211 4 -0.009335932229049604 2211 5 -0.15099081003491932 2211 6 -0.21137968058945233 2211 7 -0.24124710826354345 2211 8 -0.29458472072558806 2211 9 -0.22846173591752006 2211 10 -0.3169047959843141 2211 11 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2212 22 0.13128568479611147 2212 23 0.1491700747131901 2212 24 -0.049929734130955694 2212 25 0.08810682558569027 2213 1 0.14608632164054214 2213 2 0.22651967178055088 2213 3 0.28090718632292067 2213 4 0.27042311872676095 2213 5 0.13329218215882577 2213 6 0.09846880425903493 2213 7 0.04948987618540457 2213 8 -0.07148403844640683 2213 9 0.05015096308057565 2213 10 -0.005611846202519644 2213 11 -0.19316045084251954 2213 12 -0.025870115212910605 2213 13 -0.01159221472807559 2213 14 -0.0695182064042612 2213 15 -0.0933524659735454 2213 16 0.02095992607835484 2213 17 -0.2906481623052173 2213 18 -0.1678023939779294 2213 19 -0.1250248133795111 2213 20 -0.18336599611167514 2213 21 -0.35320036525049403 2213 22 -0.42073700515761203 2213 23 -0.29572468569881494 2213 24 -0.19886614255012347 2213 25 -0.2635426743396678 2214 1 0.3992604667855417 2214 2 0.4296938169255533 2214 3 0.40931578537067637 2214 4 0.4539319919052289 2214 5 0.6343887050270993 2214 6 0.4843474653469002 2214 7 0.42324546700923804 2214 8 0.25529781121453254 2214 9 0.34623377941299793 2214 10 0.26806078400957034 2214 11 0.29837035279332014 2214 12 0.43987797308603405 2214 13 0.5561757841176913 2214 14 0.5044898732379224 2214 15 0.4639949586405052 2214 16 0.4489429011180306 2214 17 0.3527077747889962 2214 18 0.44815489934193664 2214 19 0.430775215476352 2214 20 0.410206359969699 2214 21 0.4916921466525537 2214 22 0.344866803816565 2214 23 0.5670584624760551 2214 24 0.2838528013252244 2214 25 0.3891048512699129 2215 1 0.47053973323262 2215 2 0.5213986152875201 2215 3 0.3895205392209391 2215 4 0.41764248776564017 2215 5 0.5126758945579439 2215 6 0.5253836978760518 2215 7 0.35829398476941066 2215 8 0.45453577079790364 2215 9 0.33008493226619606 2215 10 0.35111962848566947 2215 11 0.19530616691243097 2215 12 0.3083191731216459 2215 13 0.2370945057414744 2215 14 0.2674810599200228 2215 15 0.3892117306515459 2215 16 0.30902302218987643 2215 17 0.367033422434325 2215 18 0.28340817095237014 2215 19 0.15844547275401766 2215 20 0.17358924966968914 2215 21 0.18721782990724734 2215 22 0.26712799851076596 2215 23 0.19571331870324116 2215 24 0.28788734240952607 2215 25 0.23393348119958546 2216 1 -0.16413483360462067 2216 2 -0.0526888252367641 2216 3 -1.748599579281617 2216 4 -1.3897980365184068 2216 5 -0.5027473929903081 2216 6 -0.43961136451734006 2216 7 -0.36085495140080326 2216 8 -0.21005889658695764 2216 9 -0.2866670203327786 2216 10 -0.25067586482612114 2216 11 -0.3278323553965734 2216 12 -0.2713378876558039 2216 13 -0.5083476344865474 2216 14 -0.4619596439133602 2216 15 -0.6460768948983286 2216 16 -0.4065026084771475 2216 17 -0.2566455433615289 2216 18 -0.359683668530522 2216 19 -0.2995992350294472 2216 20 -0.2286227980904343 2216 21 -0.13774132261990957 2216 22 -0.16751424944686022 2216 23 -0.4925746784239706 2216 24 -0.2546532806266555 2216 25 -0.33086407692951525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17636.0 3 17.0 4 283.0 5 1830.0 6 2509.0 7 1812.0 8 1356.0 9 1213.0 10 1030.0 11 1019.0 12 889.0 13 866.0 14 864.0 15 929.0 16 943.0 17 955.0 18 1027.0 19 1290.0 20 1653.0 21 2115.0 22 2697.0 23 3309.0 24 3848.0 25 4485.0 26 5465.0 27 5863.0 28 6948.0 29 8476.0 30 10791.0 31 14064.0 32 18231.0 33 24151.0 34 33055.0 35 46420.0 36 68414.0 37 120241.0 38 289373.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.327566647759504 19.965967101531483 10.797646057855928 27.90882019285309 2 13.417928288547351 21.20999428565757 41.88499566815977 23.487081757635313 3 18.610752249495704 25.212767163028943 29.41328958999116 26.763190997484195 4 11.469733042331677 17.914022323660504 39.08141861891294 31.534826015094886 5 12.165892612563614 39.24801316670987 35.981688981803714 12.604405238922812 6 30.546475270039796 39.056424104604886 18.222001137009663 12.17509948834565 7 27.47376667606396 32.118485231846336 22.021258661211515 18.386489430878182 8 23.773255105712106 39.85699190055158 20.199469102261556 16.170283891474757 9 25.867309936881703 16.104088524255783 20.351116751844256 37.67748478701826 10 13.779017888758268 31.03395039870827 34.980666543914566 20.2063651686189 11 33.63313683220541 23.31073712001677 24.966927188825213 18.089198858952603 12 22.713367135937457 25.649944763901082 32.78106676486418 18.855621335297286 13 28.784544002175465 22.98840318614954 26.00941001846879 22.217642793206206 14 19.4868468386808 23.904705792376348 28.314633476190608 28.293813892752233 15 21.731716264122564 33.21530597648045 23.46827637097037 21.584701388426616 16 20.32226586012863 29.75244418699181 29.51025616694261 20.41503378593695 17 20.047134403865925 30.293060422113516 28.875062671195046 20.784742502825516 18 19.787441365088718 29.15089850211879 31.472076185728206 19.589583947064288 19 22.410259678641484 27.952383987309947 28.244991608421323 21.392364725627246 20 22.191425386758368 28.994095411019728 29.25767037125687 19.556808830965032 21 21.593265569757953 27.82612637113589 27.99567695585169 22.584931103254466 22 21.475440715966048 29.952539914767296 28.37889322118156 20.193126148085096 23 20.897934326159774 29.276058153286172 28.718885655003433 21.10712186555062 24 21.82345559763535 28.091424880960137 29.8031628771248 20.281956644279713 25 21.32927304273928 28.767111646188166 29.263836266421162 20.639779044651394 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 286.0 1 286.0 2 289.0 3 292.0 4 292.0 5 292.0 6 503.5 7 715.0 8 715.0 9 715.0 10 1193.0 11 1671.0 12 1671.0 13 1671.0 14 2686.0 15 3701.0 16 3701.0 17 3701.0 18 6571.0 19 9441.0 20 9441.0 21 9441.0 22 15060.0 23 20679.0 24 20679.0 25 20679.0 26 29556.5 27 38434.0 28 38434.0 29 38434.0 30 50991.5 31 63549.0 32 63549.0 33 63549.0 34 71102.0 35 78655.0 36 78655.0 37 78655.0 38 83268.0 39 87881.0 40 87881.0 41 87881.0 42 90571.5 43 93262.0 44 93262.0 45 93262.0 46 93478.5 47 93695.0 48 93695.0 49 93695.0 50 87852.5 51 82010.0 52 82010.0 53 82010.0 54 69371.5 55 56733.0 56 56733.0 57 56733.0 58 46837.0 59 36941.0 60 36941.0 61 36941.0 62 28997.0 63 21053.0 64 21053.0 65 21053.0 66 15815.5 67 10578.0 68 10578.0 69 10578.0 70 7403.5 71 4229.0 72 4229.0 73 4229.0 74 2880.0 75 1531.0 76 1531.0 77 1531.0 78 1022.5 79 514.0 80 514.0 81 514.0 82 336.0 83 158.0 84 158.0 85 158.0 86 101.5 87 45.0 88 45.0 89 45.0 90 29.5 91 14.0 92 14.0 93 14.0 94 7.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12279287942928928 2 0.11670280582437645 3 0.01855348005217635 4 0.0 5 0.006231703223631752 6 0.3494002693795348 7 0.026909627556591657 8 0.01317155454085802 9 0.012888295303420212 10 0.005240295892599427 11 0.006939851317226269 12 9.91407331032324E-4 13 0.001557925805907938 14 1.4162961871890344E-4 15 0.002266073899502455 16 5.665184748756138E-4 17 1.4162961871890344E-4 18 4.2488885615671036E-4 19 2.832592374378069E-4 20 8.497777123134207E-4 21 0.011755258353668986 22 0.0 23 2.832592374378069E-4 24 1.4162961871890344E-4 25 0.0059484439861939445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 706067.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.529364117212 #Duplication Level Percentage of deduplicated Percentage of total 1 94.23811839754029 79.65888223746617 2 4.405994140160252 7.448717659438167 3 0.6214877518824381 1.5760189341977433 4 0.21938198480116233 0.7417687869605647 5 0.12145188653731416 0.5133125369917473 6 0.07469740132716353 0.3788474301235992 7 0.05275300825553704 0.3121424770177407 8 0.03923477675777537 0.26531925844924165 9 0.026744332190338747 0.2034613254409936 >10 0.16491080147903855 2.71884193329072 >50 0.01794849726657485 1.0842770848223227 >100 0.014257736445802156 2.4974525758150774 >500 0.0015096426781649778 0.77444566534253 >1k 0.0015096426781649778 1.8265120946434406 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1955 0.27688590459545626 No Hit GTCCTACAGTGGACATTTCTAAATT 1870 0.26484738700434945 No Hit CTGTAGGACGTGGAATATGGCAAGA 1731 0.24516087000242187 No Hit GTCCTACAGTGTGCATTTCTCATTT 1384 0.19601539230696238 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1363 0.1930411703138654 No Hit CTTTAGGACGTGAAATATGGCGAGG 1280 0.18128591196019642 No Hit GGTATCAACGCAGAGTACTTTTTTT 1177 0.16669806123214936 No Hit CTGTAGGACCTGGAATATGGCGAGA 1106 0.15664235830310722 No Hit TATCAACGCAGAGTACTTTTTTTTT 1016 0.1438956926184059 No Hit CTGAAGGACCTGGAATATGGCGAGA 756 0.10707199175149101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.4162961871890344E-4 0.0 4 0.0 0.0 0.0 2.832592374378069E-4 0.0 5 0.0 0.0 0.0 2.832592374378069E-4 0.0 6 0.0 0.0 0.0 2.832592374378069E-4 0.0 7 0.0 0.0 0.0 2.832592374378069E-4 0.0 8 0.0 0.0 0.0 2.832592374378069E-4 0.0 9 0.0 0.0 0.0 2.832592374378069E-4 0.0 10 0.0 0.0 0.0 2.832592374378069E-4 0.0 11 0.0 0.0 0.0 2.832592374378069E-4 0.0 12 0.0 0.0 0.0 2.832592374378069E-4 0.0 13 0.0 0.0 0.0 2.832592374378069E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGCGTG 25 0.006059622 18.98328 7 TTCCCGT 60 2.5926387E-5 14.233426 12 TAGGACC 445 0.0 14.116493 4 GATATAC 375 0.0 13.473077 1 GTGCTAG 50 0.0014596675 13.345972 1 GACGTGG 420 0.0 13.333495 7 GTAATCT 65 5.3487136E-5 13.178684 4 TGTAGGA 1015 0.0 13.052035 2 GTCCTAT 95 7.039307E-8 13.044935 1 GTAGGAC 985 0.0 13.04489 3 CTGTAGG 1015 0.0 12.866983 1 GGACCTG 640 0.0 12.789729 6 TTATAGT 60 4.022559E-4 12.690584 4 AGGACCT 675 0.0 12.549578 5 TCCAACG 115 5.3951226E-9 12.376892 18 GTAGACT 55 0.003022571 12.11374 4 GTTCTAG 120 9.475116E-9 11.916047 1 TTATACT 80 2.806437E-5 11.897423 4 TCAGCAC 105 2.6459566E-7 11.784115 3 AGGACGT 780 0.0 11.714385 5 >>END_MODULE