FastQCFastQC Report
Thu 26 May 2016
SRR1926824_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926824_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595532
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT14770.24801354083407776No Hit
GTCCTAAAGTGTGTATTTCTCATTT13060.2192997185709584No Hit
GTATCAACGCAGAGTACTTTTTTTT12170.20435509762699572No Hit
CTGTAGGACGTGGAATATGGCAAGA12060.2025080096451576No Hit
CTTTAGGACGTGAAATATGGCGAGG10810.18151837348790661No Hit
GTCCTACAGTGTGCATTTCTCATTT8630.14491244802966086No Hit
GTACATGGGAAGCAGTGGTATCAAC7980.13399783722789035No Hit
GGTATCAACGCAGAGTACTTTTTTT7050.1183815479268956No Hit
TATCAACGCAGAGTACTTTTTTTTT6820.11451945487396142No Hit
CCCATGTACTCTGCGTTGATACCAC6740.11317611815989737No Hit
CTGAAGGACCTGGAATATGGCGAGA5970.10024650228703076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTCGA307.800689E-418.9688517
TATGTCG307.800689E-418.9688516
TGTCGAG400.00532879614.22783218
GGCGAGG3100.013.462893519
TAGGACC1750.013.0554424
AAATGTC1750.013.0477467
AATGTCC1750.013.03895958
GTCCTAG905.12573E-712.7185161
CTATACC751.4440226E-512.6927914
TGTCCAC1950.012.64696110
GTCGTCA550.002972241712.1404011
GTAAGAC550.002994884312.1281123
GTCCTAT959.862706E-712.049121
ATGTCCA1900.011.9823389
AACAGTG2500.011.7973377
ACAGTTC657.957243E-411.7016318
GGACCTG3700.011.5720336
TTAGGAC4700.011.55672553
CTTACAC752.0114657E-411.4350773
TAAGACT752.0304669E-411.4235124
Produced by FastQC (version 0.11.5)