##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926824_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595532
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.370561111745463	31.0	28.0	31.0	22.0	34.0
2	28.499644015770773	31.0	29.0	31.0	22.0	34.0
3	28.268539725824976	31.0	28.0	33.0	16.0	34.0
4	31.865768758018042	35.0	32.0	37.0	19.0	37.0
5	31.64109065507815	35.0	32.0	37.0	19.0	37.0
6	31.40069215424192	35.0	32.0	37.0	17.0	37.0
7	31.214507364843534	35.0	32.0	37.0	17.0	37.0
8	31.030376201446774	35.0	32.0	37.0	13.0	37.0
9	33.21541747546731	37.0	33.0	39.0	17.0	39.0
10	33.41490633584761	37.0	34.0	39.0	17.0	39.0
11	33.56833050113176	38.0	34.0	39.0	19.0	39.0
12	33.49119946535199	38.0	34.0	39.0	18.0	39.0
13	33.574482983282174	38.0	34.0	39.0	19.0	39.0
14	34.59129820060047	39.0	34.0	41.0	19.0	41.0
15	34.210655346815955	39.0	34.0	41.0	17.0	41.0
16	34.26497820436182	39.0	34.0	41.0	17.0	41.0
17	34.502280314072124	39.0	34.0	41.0	18.0	41.0
18	34.54547194777107	39.0	34.0	41.0	18.0	41.0
19	33.91113827636466	39.0	34.0	41.0	10.0	41.0
20	34.241122223490926	39.0	34.0	41.0	16.0	41.0
21	34.22969714473781	39.0	34.0	41.0	13.0	41.0
22	34.253551446437804	39.0	34.0	41.0	11.0	41.0
23	34.25202340092556	39.0	34.0	41.0	9.0	41.0
24	32.93843655756534	39.0	32.0	41.0	2.0	41.0
25	33.52749810253689	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.37928808588947405
1101	2	-0.4390761857093608
1101	3	-1.1815185085981277
1101	4	-0.6003723494562578
1101	5	-0.5659293395009115
1101	6	-2.1021246466626557
1101	7	-0.746863488494192
1101	8	-0.16301841661024596
1101	9	-0.25651203663070987
1101	10	-9.305906892562962
1101	11	-4.026187784508025
1101	12	-1.544530001615211
1101	13	-0.847004967580105
1101	14	-0.7740778147571348
1101	15	-1.785707701394017
1101	16	-0.5809339618627618
1101	17	-0.455178609912231
1101	18	-0.3063824094955834
1101	19	-0.9121300846112419
1101	20	-0.20757134466126814
1101	21	-0.2084891145099803
1101	22	-0.021618369810020965
1101	23	-0.22929925178948452
1101	24	-0.2631551649522237
1101	25	-0.22025346180252825
1102	1	0.09848120364452129
1102	2	0.11823987048411055
1102	3	-0.17650448355716009
1102	4	-0.12043719704692535
1102	5	-0.14484337562195293
1102	6	-0.7202084936643303
1102	7	-0.1417246668308998
1102	8	0.18332020211274624
1102	9	0.050927358280624446
1102	10	0.3492205574454914
1102	11	0.04715997236016278
1102	12	0.2532411540365018
1102	13	-0.21159888287002104
1102	14	-0.4968193749481742
1102	15	0.2361946889861457
1102	16	0.20628313177552826
1102	17	0.03911748465328202
1102	18	-0.2794088442193754
1102	19	0.30207107121541554
1102	20	0.1559262264698944
1102	21	0.3228832401223656
1102	22	0.10176000305196453
1102	23	-0.20432243247049797
1102	24	-3.7033105192521667
1102	25	-2.007807622953454
1103	1	-0.6453664979496594
1103	2	-0.6766646121958821
1103	3	-0.3363774028107933
1103	4	-0.6526652098684202
1103	5	-1.2812519621971887
1103	6	-0.17311264789092107
1103	7	-0.44537392586130053
1103	8	-0.06580461135998661
1103	9	-0.2919602669422332
1103	10	0.010634726547010587
1103	11	-0.3770293207679529
1103	12	-0.5119867370918314
1103	13	-0.31022823975118285
1103	14	-0.5037665187623261
1103	15	-0.22696122831448662
1103	16	-0.39900685831179317
1103	17	-0.7236729987048776
1103	18	-0.7923089105007932
1103	19	-2.4174226379568218
1103	20	-1.298342545291618
1103	21	-1.0389199926624855
1103	22	-0.9814320441224496
1103	23	-0.9606797188783176
1103	24	0.4547051023695161
1103	25	-0.4908834118028196
1104	1	-0.5763105273027165
1104	2	-0.4050271449267413
1104	3	-1.5996622121820572
1104	4	-0.7137089937546577
1104	5	-0.701385155442054
1104	6	-2.2440160547083003
1104	7	-0.6669972360831551
1104	8	-0.16593822580626139
1104	9	-0.26681337295161
1104	10	-0.22180760981039782
1104	11	-0.16805609279737155
1104	12	-0.17273942400580466
1104	13	-0.3693227971270048
1104	14	-0.5007268486098226
1104	15	-0.23976575108159182
1104	16	-0.5615613512438031
1104	17	-0.44315403177152746
1104	18	-0.45987336016605695
1104	19	-1.229936531753225
1104	20	-0.47629311964654164
1104	21	-0.34171349399277773
1104	22	-0.2553451689922852
1104	23	-0.25958880723467104
1104	24	0.950331627762111
1104	25	0.33094137309196725
1105	1	0.04802461797666879
1105	2	-0.12411856669208987
1105	3	0.22700287887934678
1105	4	0.09381366337101582
1105	5	0.019939803783312726
1105	6	-0.0073488342397851625
1105	7	0.11367266135430043
1105	8	0.32964500944174446
1105	9	-0.3547988368230577
1105	10	-0.0088668985884226
1105	11	-0.14734351361735776
1105	12	-0.08945152582422367
1105	13	0.07222029762693438
1105	14	-0.05631256426611486
1105	15	0.37000518555333883
1105	16	-0.3340014411936991
1105	17	-0.43873258700390494
1105	18	-0.12166969056580257
1105	19	-0.7511171124963667
1105	20	-0.012318709972014119
1105	21	0.07158921643827654
1105	22	-0.13744504520587242
1105	23	-0.34637852711233563
1105	24	-5.239769437175443
1105	25	-2.528803238455037
1106	1	0.08826623185004223
1106	2	-0.5565976873729923
1106	3	-0.2809609206653114
1106	4	-0.5218831445184371
1106	5	-0.08153894804792827
1106	6	0.3433612880818657
1106	7	-2.9463076127044694
1106	8	-0.45823868381871335
1106	9	-0.6021686149698127
1106	10	0.14105719238216352
1106	11	-0.26634667138673507
1106	12	0.0725035392679132
1106	13	-0.2636618369835375
1106	14	-0.38836051240978264
1106	15	0.2518726979918995
1106	16	0.09641952149235777
1106	17	-0.32180608752596385
1106	18	-0.12219348021280041
1106	19	0.3963695643994214
1106	20	0.4700846173063269
1106	21	-0.08780471384567079
1106	22	-0.4456831116473765
1106	23	-0.37976809363882325
1106	24	-3.381900278210491
1106	25	-1.6936096617580105
1107	1	0.17249509048157563
1107	2	0.0015832217971798457
1107	3	0.39258630866016375
1107	4	0.32176845760455564
1107	5	0.15793906656227463
1107	6	0.3712208897280789
1107	7	-2.0096001070675413
1107	8	-1.4463092604461352
1107	9	-0.14181030184258958
1107	10	0.05281654228432586
1107	11	-0.07216713516528017
1107	12	0.3195515339791939
1107	13	-0.14433670087509398
1107	14	-0.1828949845694936
1107	15	-1.2309968972641485
1107	16	-0.5748497607666962
1107	17	-0.4598106103103987
1107	18	-0.330828709591259
1107	19	0.40330604565547645
1107	20	0.3275043345716
1107	21	-0.057195903797101266
1107	22	-0.10287856590688449
1107	23	-0.25497544310834996
1107	24	-4.107236589944897
1107	25	-1.9023177324670755
1108	1	0.4626558890627308
1108	2	0.10624536259908268
1108	3	0.5773798317083205
1108	4	0.6671569514774944
1108	5	0.7979120614023643
1108	6	-2.9173528198914376
1108	7	-1.4299589970697397
1108	8	0.35374753612034837
1108	9	0.2893913741919363
1108	10	0.592621826508271
1108	11	0.5319125729218044
1108	12	0.7512279435788827
1108	13	0.5860392816787154
1108	14	0.3774835163226413
1108	15	-0.49431649583029724
1108	16	0.4773077050348036
1108	17	0.23243880163081343
1108	18	0.05607914601687014
1108	19	0.45570677222286804
1108	20	-0.6267894322731351
1108	21	0.1844842029375826
1108	22	0.3936873850873255
1108	23	0.4734576682182734
1108	24	-0.7688158559832274
1108	25	0.03671919065930496
1109	1	0.6076012970342788
1109	2	0.74622694473587
1109	3	-0.6053243128401533
1109	4	0.6896955469566493
1109	5	0.9225523044334736
1109	6	1.0789244430447305
1109	7	1.2129514487166482
1109	8	1.4981965085835292
1109	9	0.8881613996488085
1109	10	1.0482480073949745
1109	11	0.6422133450960104
1109	12	0.37020892766436475
1109	13	0.5381179069016611
1109	14	0.8481657711348305
1109	15	-0.6930801702998579
1109	16	0.5416040421028754
1109	17	0.3572230003743755
1109	18	0.4503801934005267
1109	19	-1.0812702636655018
1109	20	0.35443069823965345
1109	21	0.7749513709794158
1109	22	1.031833531890399
1109	23	0.9333517125799204
1109	24	0.10067347171183627
1109	25	0.5624179315760713
1110	1	0.7138888326647184
1110	2	0.6706866723134297
1110	3	0.9904444222191309
1110	4	0.9330767084153422
1110	5	1.1929585850168252
1110	6	0.9986116973751997
1110	7	1.369585261400811
1110	8	1.443040322925551
1110	9	1.1217056188274768
1110	10	1.2218977942808351
1110	11	0.8499496290485453
1110	12	0.8969404466307225
1110	13	0.7906129734563763
1110	14	0.700446921362925
1110	15	1.162070667449953
1110	16	0.9696441941973788
1110	17	0.6178062432389311
1110	18	0.9845602788601013
1110	19	-1.3941806487683692
1110	20	0.44440390521974393
1110	21	0.5211578323220607
1110	22	0.8742071474506687
1110	23	0.8702891794897383
1110	24	0.02517812171798539
1110	25	0.5500631941316243
1111	1	0.8099306737146819
1111	2	0.6118181532174312
1111	3	1.223964311489155
1111	4	1.2021231942157584
1111	5	1.3134977918532016
1111	6	1.130501820852249
1111	7	1.4029060117824095
1111	8	1.7138517044789374
1111	9	0.9851991933158146
1111	10	1.080570456997549
1111	11	1.0664792509581957
1111	12	0.8596128467467068
1111	13	0.8985725110602516
1111	14	1.1001000695627425
1111	15	0.04954953629729886
1111	16	0.5209284759023163
1111	17	0.8590017330713593
1111	18	0.987698357607492
1111	19	1.5212093953813834
1111	20	0.7303596898214906
1111	21	0.845736713752359
1111	22	1.195899609168407
1111	23	0.904574987561432
1111	24	1.70246042297574
1111	25	1.3807054170468192
1112	1	0.09998338180285415
1112	2	0.139324259642283
1112	3	-1.4974178996134953
1112	4	-0.11734913156571736
1112	5	0.18064308356065695
1112	6	0.7846919325643888
1112	7	0.8871710487505844
1112	8	1.228179140703471
1112	9	-0.049405528986063985
1112	10	0.4453473458688606
1112	11	0.34580904404845825
1112	12	0.3432548800460893
1112	13	0.1558763225901103
1112	14	0.35982008050650904
1112	15	0.672444426678318
1112	16	0.5078585841203633
1112	17	-0.41442106227029285
1112	18	-0.050146643035290595
1112	19	0.6667483265180465
1112	20	0.3336579833188509
1112	21	0.5051284116516186
1112	22	0.4160374610913138
1112	23	0.511064114664066
1112	24	1.5842545416892477
1112	25	0.8940703290846344
1113	1	-0.4666964148948871
1113	2	0.04105439373693187
1113	3	-4.697182905724013
1113	4	-1.1355437635152335
1113	5	0.12657204448142068
1113	6	0.6998180650295716
1113	7	1.1935002848408907
1113	8	1.634610835925148
1113	9	1.0915346934613481
1113	10	1.2307050045486534
1113	11	0.758199753373141
1113	12	0.4577756844119918
1113	13	0.0693157510634208
1113	14	-0.28910757751117444
1113	15	0.5272195496461123
1113	16	0.7100801404891541
1113	17	0.6344372422499163
1113	18	0.4775930726153703
1113	19	1.1925275153065726
1113	20	0.8105328563671605
1113	21	0.9164642295908294
1113	22	0.9417235052149522
1113	23	0.6565179255714213
1113	24	2.07167496191758
1113	25	1.4547361226603428
1114	1	0.6285962157510596
1114	2	0.38133757301446636
1114	3	0.8059826243666137
1114	4	0.395337113065267
1114	5	0.83813007317017
1114	6	1.223501882839745
1114	7	1.599581268144977
1114	8	1.5591253792748212
1114	9	0.624355587768818
1114	10	0.4548177516861074
1114	11	0.3597974416317342
1114	12	-0.004570335160032357
1114	13	0.4619635813186562
1114	14	0.4397749451449826
1114	15	-2.2492058433318363
1114	16	0.09391875272076078
1114	17	0.2660769416150046
1114	18	0.5920631628782047
1114	19	0.8784300531572669
1114	20	0.9151366676653936
1114	21	0.7304576814657295
1114	22	0.8146432997268249
1114	23	0.27392969772560605
1114	24	1.2562423243979097
1114	25	0.8449939776971576
1115	1	-3.790024363236455
1115	2	-1.3047054231342976
1115	3	-0.34163872672829143
1115	4	0.5132032594121796
1115	5	0.9318911198873181
1115	6	1.4499677240647024
1115	7	1.3646071290912296
1115	8	1.5048813468277125
1115	9	0.7342262365250747
1115	10	-0.47458326042574583
1115	11	0.24642091369633334
1115	12	0.059284341206044644
1115	13	0.5074295434760927
1115	14	0.7187812987515585
1115	15	1.0424437687539694
1115	16	0.9117525728848577
1115	17	0.573755921689056
1115	18	0.8297094446694899
1115	19	-1.543052206977805
1115	20	0.037693598957133645
1115	21	0.13072559342688095
1115	22	0.6382981584218683
1115	23	0.3336773209372055
1115	24	-1.1740552160214506
1115	25	-0.318588135458441
1116	1	0.44324310840642767
1116	2	-0.4912330484544576
1116	3	-0.07295522874073157
1116	4	-0.22551316942060495
1116	5	0.6616357228876808
1116	6	1.0480357811448293
1116	7	1.0468976523257716
1116	8	-1.799046937109356
1116	9	-0.355285655169034
1116	10	-0.5178997624381836
1116	11	0.30737371281817616
1116	12	0.17301723546143677
1116	13	-0.1494149497547923
1116	14	0.0771450016421511
1116	15	-0.681957255761219
1116	16	-0.055493676657768276
1116	17	-0.05415187194432036
1116	18	0.14879762615503722
1116	19	0.7706221435527496
1116	20	0.3329135038235833
1116	21	0.05615259671996142
1116	22	0.3053834127211772
1116	23	0.4507150084600724
1116	24	1.3994259402171139
1116	25	0.9710335257197542
1201	1	0.1844415296479056
1201	2	0.13826820737618206
1201	3	0.4110892635448806
1201	4	0.07386537381584901
1201	5	-0.3932935917875966
1201	6	0.4885524657519369
1201	7	0.48848358702257855
1201	8	0.3858127686674635
1201	9	0.10370393937707689
1201	10	-0.2234699095497774
1201	11	-0.1509599793239076
1201	12	0.017922868758894595
1201	13	0.033751178698743445
1201	14	-0.18231391475540448
1201	15	0.5343800244684189
1201	16	-0.01693034691252393
1201	17	-0.14076543491525229
1201	18	-0.21354611263218715
1201	19	-0.67337068027215
1201	20	-0.022308598795724777
1201	21	0.06250781738734901
1201	22	0.10114372847287711
1201	23	-0.014048536570427927
1201	24	-0.826349445502295
1201	25	-0.2658893303979326
1202	1	-0.35511742840895266
1202	2	-0.45219786528434724
1202	3	-0.6641949820958288
1202	4	-1.0795400101895574
1202	5	-0.9663541211456881
1202	6	0.06940683663993497
1202	7	-0.6010167473840582
1202	8	-0.05205963206764608
1202	9	-0.06713041396482566
1202	10	0.07010749182369125
1202	11	-0.3153814558434149
1202	12	0.10260446796053202
1202	13	-0.15466558450270895
1202	14	-0.17052710572092167
1202	15	0.28643302352787003
1202	16	0.18137103135818933
1202	17	-0.04468941274954119
1202	18	-0.1765458631449235
1202	19	-0.4774329888375277
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2211	1	0.2548737297589838
2211	2	0.15606526943088284
2211	3	-0.10036065270228178
2211	4	0.40148030809398705
2211	5	0.7798259048306946
2211	6	-1.9707489768366315
2211	7	0.14827080714897534
2211	8	0.48457681846908685
2211	9	0.6159453781299717
2211	10	0.45567524614403254
2211	11	0.23177330087172976
2211	12	0.31735228523830017
2211	13	0.22474090331763819
2211	14	0.22850511149454178
2211	15	0.7434694521000935
2211	16	-0.18212464129903339
2211	17	0.1003939570979071
2211	18	-0.05660848438392918
2211	19	0.25280820603129683
2211	20	-0.02904873441877953
2211	21	-0.22346017361989112
2211	22	-3.060007593114733
2211	23	-1.5555685480109176
2211	24	0.5651094280303681
2211	25	0.09424121538676644
2212	1	-0.7333994286497614
2212	2	-0.2962451132596975
2212	3	-2.868013484514602
2212	4	-1.890227405890787
2212	5	-0.7624332188565468
2212	6	0.1240647366774077
2212	7	0.3709583210606908
2212	8	0.19484896085686287
2212	9	0.1839394396258669
2212	10	0.23275449471451992
2212	11	-0.1052591723938221
2212	12	-0.0010275766723069069
2212	13	-0.13089306075099927
2212	14	-0.32353614279080034
2212	15	-1.204109617015952
2212	16	-0.23048574835718227
2212	17	-0.11441247173749503
2212	18	-0.10435507980842118
2212	19	0.5108551666482342
2212	20	-0.8069320666693542
2212	21	-0.9579197921114755
2212	22	-0.20215467759251027
2212	23	-0.14846044827898197
2212	24	1.0882132549361785
2212	25	0.435841522962086
2213	1	-0.1983726274558819
2213	2	-0.34510730205473905
2213	3	0.4262340933527007
2213	4	-6.565798381924996
2213	5	-1.2359856329254555
2213	6	-3.215989508633882
2213	7	-1.064201750554453
2213	8	-2.65126892516696
2213	9	-0.8278097155835056
2213	10	-0.09448704729944524
2213	11	0.09267192145885161
2213	12	0.21640044585834772
2213	13	0.23955730173801015
2213	14	0.13310847196179054
2213	15	-2.4269196723400697
2213	16	-0.41785546099215765
2213	17	-0.025473262882144354
2213	18	0.18265361491513232
2213	19	0.917637247170596
2213	20	-0.5903474726428755
2213	21	0.13036123739436078
2213	22	0.18655798244368782
2213	23	0.3640356969040468
2213	24	-0.24297518377304783
2213	25	0.05082839751462842
2214	1	0.5306166357799533
2214	2	0.292121074899665
2214	3	0.26921247742697574
2214	4	0.616726608819171
2214	5	0.831468016719974
2214	6	0.626746799855848
2214	7	0.9112774167055342
2214	8	-2.353131483193902
2214	9	-0.7433965352799135
2214	10	-0.43129962383804354
2214	11	-0.14769489425042792
2214	12	-1.1202412537970474
2214	13	-0.3054260377658835
2214	14	-0.12373372323656895
2214	15	-1.3955068053107667
2214	16	-0.2855255546896487
2214	17	-0.03771943951188206
2214	18	-0.0398565664991537
2214	19	0.7363795933101969
2214	20	-0.9346928717827936
2214	21	-0.3965376319702614
2214	22	0.20321847855623787
2214	23	0.03696310270816383
2214	24	-1.292330123538946
2214	25	-0.2527059060196777
2215	1	0.8504799968259995
2215	2	0.21382150979967562
2215	3	0.6103872562997488
2215	4	0.9901939759507385
2215	5	1.2020144627680054
2215	6	1.5890283893813653
2215	7	1.4355284161413273
2215	8	0.27443845535808364
2215	9	0.6967284243804244
2215	10	0.33488728437631465
2215	11	0.5375152122901952
2215	12	-7.469320851996031E-4
2215	13	0.7227469650639335
2215	14	0.7280369411000933
2215	15	0.6792099388885546
2215	16	0.7908519762813384
2215	17	0.48549754727427086
2215	18	-0.12104961073337961
2215	19	1.0840526257773178
2215	20	-2.643915781639283
2215	21	-0.8130427570287182
2215	22	0.01946718604042985
2215	23	0.30508741254314486
2215	24	0.34235167059374305
2215	25	0.7108885063603765
2216	1	-0.8792923525864786
2216	2	-1.5550805661849552
2216	3	-0.7224162216485333
2216	4	-0.8321691977894012
2216	5	-0.7380734969704754
2216	6	-2.3467514528977205
2216	7	-10.034839936326712
2216	8	-10.956426369733471
2216	9	-6.066299839566195
2216	10	-3.5894033085374772
2216	11	-2.4196156488839513
2216	12	-2.143837374467637
2216	13	-1.9350567937264245
2216	14	-2.1040854948117484
2216	15	-3.060939525264761
2216	16	-2.1450823291400383
2216	17	-2.229460382582616
2216	18	-2.1175924447669487
2216	19	-1.389353033752215
2216	20	-2.4371893330558514
2216	21	-4.941783573492803
2216	22	-3.4406307716759876
2216	23	-3.2784729348023376
2216	24	-4.187793917938912
2216	25	-3.280186332436273
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33020.0
3	1.0
4	265.0
5	1692.0
6	2241.0
7	1753.0
8	1292.0
9	1160.0
10	1059.0
11	1107.0
12	1063.0
13	1067.0
14	999.0
15	1068.0
16	1146.0
17	1226.0
18	1331.0
19	1810.0
20	2469.0
21	2887.0
22	3692.0
23	4361.0
24	5160.0
25	5903.0
26	6591.0
27	7361.0
28	8301.0
29	9951.0
30	11714.0
31	14421.0
32	18870.0
33	25255.0
34	34329.0
35	48377.0
36	70377.0
37	111903.0
38	150310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96569501746546	19.05535102538044	12.61349181243648	25.365462144717625
2	13.689454402391476	20.50324574337695	43.21941426983804	22.58788558439353
3	18.34564368305039	25.595831574912108	30.320954608352658	25.73757013368485
4	11.067383633002857	17.74743740547807	39.5116787094606	31.673500252058478
5	11.71072211976768	39.92703892936683	36.31072950993137	12.051509440934115
6	30.616844781920083	38.292344151252685	19.096205750410228	11.994605316417003
7	26.535802286283644	32.88855377075227	23.073893549125476	17.501750393838613
8	23.744864762975467	38.641385845006305	21.19546466637158	16.418284725646647
9	26.452696461261986	15.452209401924724	20.45212542617692	37.64296871063637
10	13.766817122766467	30.31105272794771	35.866393639520176	20.055736509765648
11	33.50844340692099	22.971105158413597	25.351045874024873	18.169405560640538
12	22.534714524678044	25.83620521708754	33.07536763470982	18.55371262352459
13	28.53126605960654	23.23869151784065	25.96542377208039	22.264618650472425
14	19.615805773326077	23.707316417770073	28.4685973786923	28.208280430211545
15	22.210858134195885	33.00601400973729	23.568334836415914	21.214793019650916
16	20.46838234429663	29.17547710740984	29.762970452343	20.593170095950526
17	20.490677200372172	29.93003281706084	28.889762219064796	20.689527763502188
18	20.16585998300422	28.61756476701341	31.30044571931439	19.916129530667977
19	22.82355114757678	27.690653946605977	28.07074414034616	21.415050765471083
20	22.48124897133855	28.586879486223495	29.29006405411851	19.641807488319444
21	21.965588761346556	27.793163096507655	27.73757441913191	22.503673723013883
22	21.87323132319278	29.639706363961565	28.162960947616096	20.32410136522956
23	21.038488603900085	29.16968272975683	28.77836428891729	21.013464377425798
24	22.322110838857252	28.145357810429584	29.288376178328328	20.244155172384836
25	21.614831310469683	28.31941932309222	29.10135814847097	20.964391217967126
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	293.0
1	293.0
2	364.0
3	435.0
4	435.0
5	435.0
6	661.5
7	888.0
8	888.0
9	888.0
10	1238.0
11	1588.0
12	1588.0
13	1588.0
14	2631.0
15	3674.0
16	3674.0
17	3674.0
18	6083.5
19	8493.0
20	8493.0
21	8493.0
22	13168.5
23	17844.0
24	17844.0
25	17844.0
26	25556.0
27	33268.0
28	33268.0
29	33268.0
30	43577.5
31	53887.0
32	53887.0
33	53887.0
34	59710.5
35	65534.0
36	65534.0
37	65534.0
38	69438.0
39	73342.0
40	73342.0
41	73342.0
42	75206.0
43	77070.0
44	77070.0
45	77070.0
46	77924.5
47	78779.0
48	78779.0
49	78779.0
50	73641.5
51	68504.0
52	68504.0
53	68504.0
54	58036.5
55	47569.0
56	47569.0
57	47569.0
58	39384.5
59	31200.0
60	31200.0
61	31200.0
62	24598.0
63	17996.0
64	17996.0
65	17996.0
66	13564.5
67	9133.0
68	9133.0
69	9133.0
70	6491.5
71	3850.0
72	3850.0
73	3850.0
74	2667.0
75	1484.0
76	1484.0
77	1484.0
78	996.0
79	508.0
80	508.0
81	508.0
82	328.0
83	148.0
84	148.0
85	148.0
86	93.0
87	38.0
88	38.0
89	38.0
90	22.0
91	6.0
92	6.0
93	6.0
94	3.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2041871805377377
2	0.12644156821128
3	0.13013574417495619
4	0.07254018255945945
5	0.024515895031669163
6	0.021997138692799043
7	0.23172558317605102
8	0.22719182176608477
9	0.020485884889476974
10	0.03878884761859984
11	0.016287957658026774
12	0.01746337728283283
13	0.01578420639025275
14	0.01746337728283283
15	0.014944620943962709
16	0.020317967800218963
17	0.018470879818380876
18	0.015112538033220716
19	0.02468381212092717
20	0.01645587474728478
21	0.014608786765446692
22	0.016959626015058803
23	0.01830296272912287
24	0.015280455122478727
25	0.014944620943962709
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	595532.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.16008274033915
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.30517652302558	80.31036635543147
2	4.300090419824013	7.323921118863053
3	0.627038719659456	1.6019600774278682
4	0.25047998683610523	0.8532358561504713
5	0.12671244940296714	0.5395421337693859
6	0.08323958973380778	0.4253214209401774
7	0.05370851808483398	0.3201675290775824
8	0.037032954123301945	0.2522983549807662
9	0.02512104054694036	0.19253789023507697
>10	0.15423926678226488	2.49162742043015
>50	0.020952252513245437	1.2649970759240265
>100	0.013638674186120322	2.4496930382528297
>500	0.0015812955577496502	0.9160468297885958
>1k	9.883097235935314E-4	1.0582848987285742
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	1477	0.24801354083407776	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1306	0.2192997185709584	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1217	0.20435509762699572	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1206	0.2025080096451576	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1081	0.18151837348790661	No Hit
GTCCTACAGTGTGCATTTCTCATTT	863	0.14491244802966086	No Hit
GTACATGGGAAGCAGTGGTATCAAC	798	0.13399783722789035	No Hit
GGTATCAACGCAGAGTACTTTTTTT	705	0.1183815479268956	No Hit
TATCAACGCAGAGTACTTTTTTTTT	682	0.11451945487396142	No Hit
CCCATGTACTCTGCGTTGATACCAC	674	0.11317611815989737	No Hit
CTGAAGGACCTGGAATATGGCGAGA	597	0.10024650228703076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGTCGA	30	7.800689E-4	18.96885	17
TATGTCG	30	7.800689E-4	18.96885	16
TGTCGAG	40	0.005328796	14.227832	18
GGCGAGG	310	0.0	13.4628935	19
TAGGACC	175	0.0	13.055442	4
AAATGTC	175	0.0	13.047746	7
AATGTCC	175	0.0	13.0389595	8
GTCCTAG	90	5.12573E-7	12.718516	1
CTATACC	75	1.4440226E-5	12.692791	4
TGTCCAC	195	0.0	12.646961	10
GTCGTCA	55	0.0029722417	12.140401	1
GTAAGAC	55	0.0029948843	12.128112	3
GTCCTAT	95	9.862706E-7	12.04912	1
ATGTCCA	190	0.0	11.982338	9
AACAGTG	250	0.0	11.797337	7
ACAGTTC	65	7.957243E-4	11.701631	8
GGACCTG	370	0.0	11.572033	6
TTAGGAC	470	0.0	11.5567255	3
CTTACAC	75	2.0114657E-4	11.435077	3
TAAGACT	75	2.0304669E-4	11.423512	4
>>END_MODULE