FastQCFastQC Report
Thu 26 May 2016
SRR1926824_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926824_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595532
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21390.35917465392287906No Hit
GTCCTACAGTGGACATTTCTAAATT16010.26883525990207074No Hit
CTGTAGGACGTGGAATATGGCAAGA15300.25691314656475217No Hit
GTCCTAAAGTGTGTATTTCTCATTT13870.23290100280085707No Hit
GGTATCAACGCAGAGTACTTTTTTT13860.23273308571159904No Hit
CTTTAGGACGTGAAATATGGCGAGG12900.21661304514283028No Hit
TATCAACGCAGAGTACTTTTTTTTT12050.2023400925558996No Hit
GTACATGGGAAGCAGTGGTATCAAC9470.15901748352733355No Hit
GTCCTACAGTGTGCATTTCTCATTT8530.14323327713708078No Hit
CCCATGTACTCTGCGTTGATACCAC7540.126609485300538No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7530.12644156821128No Hit
CTGAAGGACCTGGAATATGGCGAGA7500.12593781694350598No Hit
CTGTAGGACCTGGAATATGGCGAGA6710.11267236689212336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCCGT453.5721783E-516.8671712
TAGGACC2300.014.9042554
CCAACGA456.819877E-414.75877419
ATTCCCG551.978434E-413.80041211
CTTATGC500.001470639613.332153
TAATACT801.9419876E-613.0929744
TCTAGGA957.0187525E-813.046822
GAGTAAG751.4189431E-512.7144311
TAAGACT604.0058815E-412.6962164
ATAGGAC1001.392109E-712.3798543
ACAATTC701.10187844E-412.1985798
TCCAACG701.10187844E-412.19857918
CTAATAC951.0063868E-612.0290093
GGCGAGG3500.011.6564219
TCCTATA1156.697155E-811.6068692
CTAGGAC752.0238006E-411.4275573
TAGCCTG752.025386E-411.4265955
TATACAG1751.8189894E-1211.4265955
TTAGGAC4800.011.308523
GTCCTAT1104.712765E-711.2696091
Produced by FastQC (version 0.11.5)