##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926824_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 595532 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.72553448009511 31.0 30.0 33.0 25.0 34.0 2 30.083362438962137 31.0 30.0 33.0 26.0 34.0 3 30.150923879825097 31.0 30.0 34.0 26.0 34.0 4 33.7131338030534 35.0 35.0 37.0 28.0 37.0 5 33.68451065601848 37.0 35.0 37.0 28.0 37.0 6 33.653273039903816 37.0 35.0 37.0 29.0 37.0 7 33.64521805713211 37.0 35.0 37.0 28.0 37.0 8 33.665416803798955 37.0 35.0 37.0 29.0 37.0 9 35.184109334175155 39.0 35.0 39.0 29.0 39.0 10 35.05400885258894 39.0 35.0 39.0 28.0 39.0 11 35.081107312453405 39.0 35.0 39.0 29.0 39.0 12 34.984578494522545 39.0 35.0 39.0 28.0 39.0 13 34.988568204563315 39.0 35.0 39.0 28.0 39.0 14 36.0660820913066 40.0 36.0 41.0 28.0 41.0 15 36.056915497404 40.0 36.0 41.0 28.0 41.0 16 36.04653654211696 40.0 36.0 41.0 28.0 41.0 17 36.03307462907115 40.0 36.0 41.0 28.0 41.0 18 36.009519219790036 40.0 36.0 41.0 28.0 41.0 19 36.07807808816319 40.0 36.0 41.0 27.0 41.0 20 36.044907746351164 40.0 36.0 41.0 27.0 41.0 21 35.994593069725894 40.0 36.0 41.0 27.0 41.0 22 35.902732346876405 40.0 36.0 41.0 27.0 41.0 23 35.82463410866251 40.0 36.0 41.0 27.0 41.0 24 35.771090386410805 40.0 36.0 41.0 27.0 41.0 25 35.6518726113794 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18830329148913094 1101 2 0.15697973195835146 1101 3 0.4780961325457689 1101 4 0.35137177056276414 1101 5 0.4313461768950546 1101 6 0.38414753971671445 1101 7 0.23922806455890822 1101 8 0.21498924236663441 1101 9 0.4408603674992335 1101 10 0.0594900179429132 1101 11 0.4111750829612646 1101 12 0.19861981585952293 1101 13 0.4256515228566897 1101 14 0.41192147471878826 1101 15 0.40894732930681954 1101 16 0.31656562231997043 1101 17 0.4388638150237085 1101 18 0.43084115486038144 1101 19 0.30670225841296883 1101 20 0.5519964682663314 1101 21 0.2637689183642209 1101 22 0.2648197631901894 1101 23 0.34212485848635765 1101 24 0.327275581125285 1101 25 0.4413270341313833 1102 1 -0.7615984457498328 1102 2 -0.655657383956715 1102 3 -0.6292861125104778 1102 4 -0.7604681631900547 1102 5 -0.6120632201739724 1102 6 -0.34848794334534716 1102 7 -0.5901306324243976 1102 8 -0.5078981082710499 1102 9 -0.63229611230296 1102 10 -0.7002391617233954 1102 11 -0.5982523104804969 1102 12 -0.5292943893784354 1102 13 -0.6514937835656767 1102 14 -0.6183936075944985 1102 15 -0.7752139965698177 1102 16 -0.6418199525900761 1102 17 -0.7590752912225369 1102 18 -0.6920426968065883 1102 19 -0.6154654705800837 1102 20 -0.7923628993530301 1102 21 -0.8895898019455544 1102 22 -0.7541143336963145 1102 23 -0.7494746959780372 1102 24 -0.6070405742056906 1102 25 -0.7028282268626782 1103 1 -0.7080924420234211 1103 2 -0.617081831995506 1103 3 -0.3907875935044878 1103 4 -0.5865532704277285 1103 5 -0.5981275804991668 1103 6 -0.5128020723944502 1103 7 -0.3888123291815688 1103 8 -0.5974973596808582 1103 9 -0.48733355953479673 1103 10 -0.6502764376396115 1103 11 -0.6044586619185424 1103 12 -0.5308834894515684 1103 13 -0.5431041622204589 1103 14 -0.5138993490307797 1103 15 -0.40100278685704893 1103 16 -0.4850519812860341 1103 17 -0.5463367342199206 1103 18 -0.3341891752072286 1103 19 -0.35608416808513965 1103 20 -0.4482039940716618 1103 21 -0.41238127775283573 1103 22 -0.5046728816959956 1103 23 -0.6697904838349444 1103 24 -0.8831021107768464 1103 25 -0.7382976493872562 1104 1 -0.31604870259733175 1104 2 -0.1314588400147123 1104 3 -0.21492196611329106 1104 4 -0.31685566963773937 1104 5 -0.17924044141611972 1104 6 -0.21619087171304585 1104 7 -0.2081379857292589 1104 8 -0.14942096310569752 1104 9 -0.10940123724083861 1104 10 0.0018919306027385119 1104 11 0.015038517202036417 1104 12 0.16325408896793903 1104 13 0.15419242675015 1104 14 -0.053412721005443586 1104 15 -0.11690360796579569 1104 16 0.011036695650147976 1104 17 0.0449598407106393 1104 18 0.19721021966675067 1104 19 0.10711920901414373 1104 20 0.22922241853255088 1104 21 0.26612855847001526 1104 22 0.36775574221560703 1104 23 0.3178285348125769 1104 24 0.20807044373948003 1104 25 0.28247693999987433 1105 1 -0.17288117441778894 1105 2 -0.26280230870190024 1105 3 -0.16651890444706652 1105 4 -0.2777775391167836 1105 5 -0.27851761300187405 1105 6 -0.4586308914627111 1105 7 -0.5859954277731205 1105 8 -0.7285916175356775 1105 9 -0.49058383039069753 1105 10 -0.38700449831133454 1105 11 -0.34335356982753495 1105 12 -0.3380340853345629 1105 13 -0.35892214705252456 1105 14 -0.42505861230272046 1105 15 -0.6621078789480563 1105 16 -0.33553952191441994 1105 17 -0.4130756291278317 1105 18 -0.2215131188692041 1105 19 -0.304079834390663 1105 20 -0.45037084130893135 1105 21 -0.4253823611764531 1105 22 -0.16339444182600715 1105 23 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2214 11 -0.11744510448244938 2214 12 0.03983186295392471 2214 13 -0.0347295904656022 2214 14 -0.1268535817033225 2214 15 -0.2502897769891135 2214 16 -0.19719803850251338 2214 17 -0.3955798017728469 2214 18 -0.3487568306199549 2214 19 -0.4277658172315739 2214 20 -0.3766248309432072 2214 21 -0.29813864942570234 2214 22 -0.22577914505578178 2214 23 -0.07939781597749374 2214 24 -0.27755615311821913 2214 25 -0.0043220703412529815 2215 1 0.35976396945331857 2215 2 0.29685177370007665 2215 3 0.26160160154723044 2215 4 0.5516936426158026 2215 5 0.31118173980410546 2215 6 0.4970139515209482 2215 7 0.44797282002085126 2215 8 0.2888278207086046 2215 9 0.17129647589670327 2215 10 0.29086746092356464 2215 11 0.12018241825595055 2215 12 0.3151262053021213 2215 13 0.3202254376678866 2215 14 0.3385662691441951 2215 15 0.3219187730441462 2215 16 0.2784379012305749 2215 17 0.2544288698831352 2215 18 0.10362150824201422 2215 19 0.13854161602411352 2215 20 0.11396587348065879 2215 21 0.5531951390780989 2215 22 0.4160781699799969 2215 23 0.385417214307445 2215 24 0.21658773490106853 2215 25 0.26159480909199573 2216 1 -0.9858220356243059 2216 2 -0.9178168888176188 2216 3 -2.015175286273209 2216 4 -1.7330675854504314 2216 5 -1.4275171750221105 2216 6 -0.8688291925622664 2216 7 -0.903909664420496 2216 8 -0.9902744395084326 2216 9 -0.7506631277789566 2216 10 -0.9850514543519964 2216 11 -0.8056556145014682 2216 12 -0.8327048513381143 2216 13 -0.9921846928675109 2216 14 -1.2444913708836651 2216 15 -1.1261661648335846 2216 16 -0.9392441967720799 2216 17 -1.0633790453699632 2216 18 -0.9316929787156809 2216 19 -1.0140637559231322 2216 20 -1.1914498851299626 2216 21 -1.1093309792350539 2216 22 -1.0386191945979562 2216 23 -1.0999860187465416 2216 24 -1.4659076592888738 2216 25 -1.4623992145886078 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15533.0 3 6.0 4 230.0 5 1648.0 6 2203.0 7 1609.0 8 1206.0 9 1125.0 10 936.0 11 920.0 12 810.0 13 776.0 14 749.0 15 769.0 16 806.0 17 836.0 18 985.0 19 1127.0 20 1465.0 21 1887.0 22 2341.0 23 2888.0 24 3307.0 25 4012.0 26 4712.0 27 5267.0 28 6074.0 29 7584.0 30 9498.0 31 12258.0 32 16215.0 33 21403.0 34 28633.0 35 40268.0 36 60200.0 37 104290.0 38 230956.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53831929665305 19.99243532200313 10.351337266965892 28.117908114377933 2 13.615336773629624 21.67218570876267 40.99645324502866 23.716024272579045 3 18.185054815133274 26.297828890799657 29.41510371883061 26.102012575236454 4 11.491573920461033 18.31958652095941 38.532774057481376 31.65606550109818 5 11.948171023531605 39.82387820593555 35.74023042937244 12.48772034116041 6 30.226413759054783 39.25415058191554 18.32153550889597 12.197900150133705 7 27.026877232701445 32.7205913226326 22.42887685984975 17.823654584816204 8 24.262143666820606 39.36470884587934 20.052059280406397 16.321088206893656 9 26.426387090598567 15.62472185976526 20.00728832373592 37.94160272590025 10 13.944108035855221 30.85275379257304 34.900571950576484 20.302566220995256 11 33.48771210988993 23.158663655217 25.040092022737003 18.31353221215607 12 22.94636020472589 25.970735213121802 32.3989804005857 18.68392418156661 13 28.707562726060658 23.244157266013573 25.939757458112663 22.108522549813106 14 19.98465237804182 23.755398534419644 28.182700509796284 28.077248577742253 15 22.031743792798874 32.92293431936566 23.524393889017627 21.520927998817836 16 20.521922526023083 29.33324825491286 29.506035810178847 20.63879340888521 17 20.29899316913281 29.98411504335619 28.808359584371622 20.908532203139377 18 20.080264638221415 28.5641361476332 31.444763487985494 19.910835726159892 19 22.494752573338033 27.58265746477927 28.517958793007907 21.404631168874783 20 22.64612243253046 28.4695262019586 29.253536357652372 19.630815007858573 21 21.85938720159875 27.512784126690903 28.012561611513693 22.615267060196654 22 22.056581342396377 29.529395565645505 27.99278628184548 20.42123681011264 23 20.788170557032295 29.348430224455154 28.806560867528308 21.056838350984247 24 22.06413761141299 27.826884197658565 29.692107225136517 20.41687096579193 25 21.41006372742462 28.471801244322048 29.234851092770004 20.88328393548333 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 308.0 1 308.0 2 295.5 3 283.0 4 283.0 5 283.0 6 547.5 7 812.0 8 812.0 9 812.0 10 1171.0 11 1530.0 12 1530.0 13 1530.0 14 2354.0 15 3178.0 16 3178.0 17 3178.0 18 5592.0 19 8006.0 20 8006.0 21 8006.0 22 12628.0 23 17250.0 24 17250.0 25 17250.0 26 24799.0 27 32348.0 28 32348.0 29 32348.0 30 42517.5 31 52687.0 32 52687.0 33 52687.0 34 58869.5 35 65052.0 36 65052.0 37 65052.0 38 68620.0 39 72188.0 40 72188.0 41 72188.0 42 74701.5 43 77215.0 44 77215.0 45 77215.0 46 78639.5 47 80064.0 48 80064.0 49 80064.0 50 74979.0 51 69894.0 52 69894.0 53 69894.0 54 59296.5 55 48699.0 56 48699.0 57 48699.0 58 40182.0 59 31665.0 60 31665.0 61 31665.0 62 25058.0 63 18451.0 64 18451.0 65 18451.0 66 14045.5 67 9640.0 68 9640.0 69 9640.0 70 6804.5 71 3969.0 72 3969.0 73 3969.0 74 2751.0 75 1533.0 76 1533.0 77 1533.0 78 1037.5 79 542.0 80 542.0 81 542.0 82 346.0 83 150.0 84 150.0 85 150.0 86 100.5 87 51.0 88 51.0 89 51.0 90 34.0 91 17.0 92 17.0 93 17.0 94 8.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11116111308880126 2 0.10444442951848096 3 0.012761698783608607 4 0.0 5 0.005877098124030279 6 0.3460771209607544 7 0.020317967800218963 8 0.009571274087706454 9 0.009907108266222469 10 0.004365844320708207 11 0.004533761409966215 12 6.716683570320319E-4 13 0.0010075025355480477 14 0.0 15 0.0023508392496121113 16 5.037512677740239E-4 17 0.0 18 3.3583417851601596E-4 19 3.3583417851601596E-4 20 6.716683570320319E-4 21 0.011250444980286534 22 0.0 23 1.6791708925800798E-4 24 0.0 25 0.004533761409966215 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 595532.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.61252260626823 #Duplication Level Percentage of deduplicated Percentage of total 1 93.08133268999383 76.89683701073687 2 5.300001763858623 8.756930310600639 3 0.771030534528631 1.910903323916088 4 0.27549544540609955 0.9103749484614131 5 0.1381221868948153 0.5705311143637568 6 0.0840142723191279 0.4164378582727876 7 0.058004809632401844 0.33543465529203575 8 0.04611629691523622 0.3047826897141864 9 0.028579567187232452 0.21249271262993422 >10 0.1753574619360927 2.7665296496030756 >50 0.023212293574557882 1.3594952526738562 >100 0.016085668940409727 2.9913204991003663 >500 0.0012216963752029665 0.7953080649858739 >1k 0.001425312437736794 1.7726219096491413 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2139 0.35917465392287906 No Hit GTCCTACAGTGGACATTTCTAAATT 1601 0.26883525990207074 No Hit CTGTAGGACGTGGAATATGGCAAGA 1530 0.25691314656475217 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1387 0.23290100280085707 No Hit GGTATCAACGCAGAGTACTTTTTTT 1386 0.23273308571159904 No Hit CTTTAGGACGTGAAATATGGCGAGG 1290 0.21661304514283028 No Hit TATCAACGCAGAGTACTTTTTTTTT 1205 0.2023400925558996 No Hit GTACATGGGAAGCAGTGGTATCAAC 947 0.15901748352733355 No Hit GTCCTACAGTGTGCATTTCTCATTT 853 0.14323327713708078 No Hit CCCATGTACTCTGCGTTGATACCAC 754 0.126609485300538 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 753 0.12644156821128 No Hit CTGAAGGACCTGGAATATGGCGAGA 750 0.12593781694350598 No Hit CTGTAGGACCTGGAATATGGCGAGA 671 0.11267236689212336 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.6791708925800798E-4 0.0 10 0.0 0.0 0.0 3.3583417851601596E-4 0.0 11 0.0 0.0 0.0 3.3583417851601596E-4 0.0 12 0.0 0.0 0.0 3.3583417851601596E-4 0.0 13 0.0 0.0 0.0 3.3583417851601596E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCCGT 45 3.5721783E-5 16.86717 12 TAGGACC 230 0.0 14.904255 4 CCAACGA 45 6.819877E-4 14.758774 19 ATTCCCG 55 1.978434E-4 13.800412 11 CTTATGC 50 0.0014706396 13.33215 3 TAATACT 80 1.9419876E-6 13.092974 4 TCTAGGA 95 7.0187525E-8 13.04682 2 GAGTAAG 75 1.4189431E-5 12.714431 1 TAAGACT 60 4.0058815E-4 12.696216 4 ATAGGAC 100 1.392109E-7 12.379854 3 ACAATTC 70 1.10187844E-4 12.198579 8 TCCAACG 70 1.10187844E-4 12.198579 18 CTAATAC 95 1.0063868E-6 12.029009 3 GGCGAGG 350 0.0 11.65642 19 TCCTATA 115 6.697155E-8 11.606869 2 CTAGGAC 75 2.0238006E-4 11.427557 3 TAGCCTG 75 2.025386E-4 11.426595 5 TATACAG 175 1.8189894E-12 11.426595 5 TTAGGAC 480 0.0 11.30852 3 GTCCTAT 110 4.712765E-7 11.269609 1 >>END_MODULE