##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926823_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6472 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.42846106304079 10.0 2.0 28.0 2.0 31.0 2 14.59301606922126 10.0 2.0 29.0 2.0 31.0 3 14.627008652657603 10.0 2.0 28.0 2.0 31.0 4 16.485630407911 10.0 2.0 32.0 2.0 37.0 5 16.413627935723113 10.0 2.0 33.0 2.0 37.0 6 15.904202719406674 8.0 2.0 32.0 2.0 37.0 7 15.676606922126082 8.0 2.0 32.0 2.0 37.0 8 15.624227441285537 2.0 2.0 32.0 2.0 37.0 9 16.31844870210136 2.0 2.0 34.0 2.0 39.0 10 16.365883807169343 2.0 2.0 34.0 2.0 39.0 11 16.376390605686034 2.0 2.0 34.0 2.0 39.0 12 16.26313349814586 2.0 2.0 34.0 2.0 39.0 13 16.288009888751546 2.0 2.0 34.0 2.0 39.0 14 16.500309023485784 2.0 2.0 35.0 2.0 40.0 15 16.256644004944377 2.0 2.0 34.0 2.0 40.0 16 16.137360939431396 2.0 2.0 34.0 2.0 40.0 17 16.132107540173052 2.0 2.0 35.0 2.0 40.0 18 16.061959208899875 2.0 2.0 35.0 2.0 40.0 19 15.75571693448702 2.0 2.0 34.0 2.0 40.0 20 15.703028430160693 2.0 2.0 34.0 2.0 40.0 21 15.644313967861557 2.0 2.0 34.0 2.0 40.0 22 15.537391841779975 2.0 2.0 34.0 2.0 40.0 23 15.38627935723115 2.0 2.0 34.0 2.0 40.0 24 14.610939431396787 2.0 2.0 33.0 2.0 40.0 25 14.781829419035846 2.0 2.0 34.0 2.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -3.6309866782523077 1101 2 -2.274996574605952 1101 3 -3.6455142947330472 1101 4 -2.137058167526911 1101 5 -0.49456589417527397 1101 6 -0.8220045579420603 1101 7 -2.228962400446777 1101 8 -2.31220246767122 1101 9 -3.6134817873099063 1101 10 -7.653792301448549 1101 11 -4.707789996461873 1101 12 -3.020917363886106 1101 13 -2.6564759025696603 1101 14 -4.26416101259851 1101 15 -4.149461259226886 1101 16 -3.6660205679736944 1101 17 -3.349788492757243 1101 18 -3.5912431752275484 1101 19 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-2.7134248563936065 2216 18 -4.0457886297730035 2216 19 -7.975574599012095 2216 20 -7.726709791944163 2216 21 -5.357657663517035 2216 22 -4.291142451298709 2216 23 -5.601156699203576 2216 24 -5.604492902930405 2216 25 -5.213515651015655 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 2994.0 3 1.0 4 16.0 5 75.0 6 85.0 7 58.0 8 57.0 9 43.0 10 36.0 11 40.0 12 49.0 13 40.0 14 47.0 15 46.0 16 38.0 17 32.0 18 43.0 19 66.0 20 86.0 21 93.0 22 83.0 23 93.0 24 117.0 25 99.0 26 97.0 27 99.0 28 84.0 29 94.0 30 101.0 31 93.0 32 97.0 33 138.0 34 137.0 35 160.0 36 206.0 37 293.0 38 536.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.44238205723124 21.206496519721576 11.337973704563032 31.013147718484145 2 14.43155452436195 19.907192575406032 38.12838360402166 27.532869296210364 3 18.298530549110595 24.841453982985307 29.404485692188707 27.45552977571539 4 12.368583797155226 20.77922077922078 33.286951144094004 33.56524427952999 5 12.764642250038635 39.22114047287899 33.58059032607016 14.433626951012208 6 30.82985628187297 36.30041724617524 17.756142790913305 15.113583681038477 7 25.817449248411588 34.681543468154345 20.61056872772354 18.890438555710524 8 23.4856700232378 37.552285050348566 19.845081332300545 19.11696359411309 9 25.56019162416937 17.02982537474888 19.286045433472417 38.12393756760933 10 14.204018547140649 31.298299845440496 31.23647604327666 23.261205564142195 11 31.46345232576109 24.772059959820737 22.160407974038016 21.604079740380158 12 20.67686601761706 27.012826456498225 30.582599289136148 21.727708236748573 13 26.734662339669296 24.33936022253129 25.05022407664967 23.875753361149744 14 20.445062586926284 23.458507185906353 27.028280018544276 29.068150208623088 15 23.319425127491886 31.123473960747955 21.279554937413074 24.27754597434709 16 22.917632514294546 29.732653376603306 24.895688456189152 22.454025652912996 17 21.851336733116984 29.79446762478751 25.54473806212332 22.809457579972182 18 21.604079740380158 28.171843609952095 26.78102302580745 23.4430536238603 19 22.22222222222222 28.01730798949158 26.920105084221913 22.840364704064285 20 22.77855045588008 28.21820429609025 26.03925204759697 22.9639932004327 21 23.14943594498532 27.87822593107711 26.889197960129813 22.08314016380776 22 22.97944676247875 28.712718281563898 25.776541492814093 22.531293463143253 23 20.87776232421573 29.593571318188843 26.070159171689074 23.458507185906353 24 23.690310616597127 27.151908514912687 26.410137536702212 22.747643331787977 25 21.77070457354759 28.352904820766376 27.1168108776267 22.759579728059332 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 29.0 1 29.0 2 25.5 3 22.0 4 22.0 5 22.0 6 18.0 7 14.0 8 14.0 9 14.0 10 18.5 11 23.0 12 23.0 13 23.0 14 36.5 15 50.0 16 50.0 17 50.0 18 60.5 19 71.0 20 71.0 21 71.0 22 111.5 23 152.0 24 152.0 25 152.0 26 191.0 27 230.0 28 230.0 29 230.0 30 312.0 31 394.0 32 394.0 33 394.0 34 481.5 35 569.0 36 569.0 37 569.0 38 644.0 39 719.0 40 719.0 41 719.0 42 758.5 43 798.0 44 798.0 45 798.0 46 809.0 47 820.0 48 820.0 49 820.0 50 808.0 51 796.0 52 796.0 53 796.0 54 746.5 55 697.0 56 697.0 57 697.0 58 584.0 59 471.0 60 471.0 61 471.0 62 376.0 63 281.0 64 281.0 65 281.0 66 236.5 67 192.0 68 192.0 69 192.0 70 138.5 71 85.0 72 85.0 73 85.0 74 59.0 75 33.0 76 33.0 77 33.0 78 26.0 79 19.0 80 19.0 81 19.0 82 11.0 83 3.0 84 3.0 85 3.0 86 2.5 87 2.0 88 2.0 89 2.0 90 2.0 91 2.0 92 2.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10815822002472188 2 0.10815822002472188 3 0.10815822002472188 4 0.061804697156983925 5 0.015451174289245981 6 0.015451174289245981 7 0.2935723114956737 8 0.2626699629171817 9 0.015451174289245981 10 0.030902348578491962 11 0.015451174289245981 12 0.015451174289245981 13 0.015451174289245981 14 0.015451174289245981 15 0.015451174289245981 16 0.015451174289245981 17 0.015451174289245981 18 0.015451174289245981 19 0.015451174289245981 20 0.015451174289245981 21 0.015451174289245981 22 0.015451174289245981 23 0.015451174289245981 24 0.015451174289245981 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 6472.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.17676143386898 #Duplication Level Percentage of deduplicated Percentage of total 1 99.07145105445389 97.26514215080346 2 0.7869058860560277 1.5451174289245984 3 0.03147623544224111 0.0927070457354759 4 0.015738117721120555 0.061804697156983925 5 0.015738117721120555 0.07725587144622992 6 0.03147623544224111 0.1854140914709518 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.047214353163361665 0.7725587144622992 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAA 26 0.40173053152039556 No Hit GGTATCAACGCAGAGTACTTTTTTT 14 0.21631644004944375 No Hit GTATCAACGCAGAGTACTTTTTTTT 10 0.15451174289245984 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE